FastQCFastQC Report
Fri 10 Feb 2017
SRR2031511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346551
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.13835346748842042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.10226125857839771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.048.5326081
GTATCAA25350.044.595661
TCAACGC28600.037.8671344
CAACGCA30300.035.899345
ATCAACG30200.035.8609283
AACGCAG31950.034.788736
TATCAAC33900.031.9469012
ACGCAGA35750.030.9580427
CGCAGAG36050.030.5686558
GTACATG37700.028.222811
TACATGG38100.027.6771662
ACATGGG38000.026.6253
TATACCG908.9483487E-426.3888875
CATGGGG26100.024.022994
GCAGAGT45350.023.8809269
AGAGTAC38150.021.85124410-11
CAGAGTA41500.020.08734910-11
GGACCGT1200.00476391719.7916686
GAGTACT24850.019.49698312-13
AGTACTT28050.018.96613312-13
Produced by FastQC (version 0.11.5)