##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031511_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1346551 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.980911231732033 34.0 31.0 34.0 30.0 34.0 2 32.1487444589919 34.0 31.0 34.0 30.0 34.0 3 32.255011507176484 34.0 31.0 34.0 30.0 34.0 4 35.4967483593269 37.0 35.0 37.0 33.0 37.0 5 35.47600128030799 37.0 35.0 37.0 33.0 37.0 6 35.59765875930432 37.0 36.0 37.0 33.0 37.0 7 35.62239380461639 37.0 36.0 37.0 33.0 37.0 8 35.66902479000053 37.0 36.0 37.0 35.0 37.0 9 37.46860386275752 39.0 38.0 39.0 35.0 39.0 10-11 37.45333596722293 39.0 38.0 39.0 35.0 39.0 12-13 37.42032867674526 39.0 38.0 39.0 35.0 39.0 14-15 38.89412023755506 41.0 39.0 41.0 35.5 41.0 16-17 38.8920939496536 41.0 39.0 41.0 35.5 41.0 18-19 38.8455286877363 41.0 39.0 41.0 35.0 41.0 20-21 38.798203335781565 41.0 39.0 41.0 35.0 41.0 22-23 38.72128497175376 41.0 39.0 41.0 34.0 41.0 24-25 38.66883913048967 41.0 39.0 41.0 34.0 41.0 26-27 38.56116181266064 40.5 39.0 41.0 34.0 41.0 28-29 38.45607927215531 40.0 38.0 41.0 34.0 41.0 30-31 38.3064299087075 40.0 38.0 41.0 34.0 41.0 32-33 38.20840428620973 40.0 38.0 41.0 33.5 41.0 34-35 38.08132592081547 40.0 38.0 41.0 33.0 41.0 36-37 37.95237907810399 40.0 38.0 41.0 33.0 41.0 38-39 37.81115123006852 40.0 38.0 41.0 32.5 41.0 40-41 37.69335843945012 40.0 38.0 41.0 32.0 41.0 42-43 37.54463031849518 40.0 38.0 41.0 32.0 41.0 44-45 37.74861182383735 40.0 38.0 41.0 32.5 41.0 46-47 37.654858598003344 40.0 38.0 41.0 32.0 41.0 48-49 37.54392629763002 40.0 38.0 41.0 31.5 41.0 50-51 36.49778285412138 39.0 36.0 40.0 30.0 40.5 52-53 36.569840280836004 39.0 36.5 40.0 30.5 40.5 54-55 37.37558436331041 40.0 37.0 41.0 31.0 41.0 56-57 37.43116636503184 40.0 37.0 41.0 31.0 41.0 58-59 37.24094371472005 40.0 37.0 41.0 31.0 41.0 60-61 37.067751240019874 40.0 36.0 41.0 31.0 41.0 62-63 36.815777865079006 39.0 36.0 41.0 30.5 41.0 64-65 36.54367045882406 39.0 35.0 41.0 30.0 41.0 66-67 36.2516763197235 39.0 35.0 41.0 30.0 41.0 68-69 35.94926296887381 37.5 35.0 40.5 29.0 41.0 70-71 35.58974706490879 37.0 35.0 40.0 29.0 41.0 72-73 35.2263163444979 37.0 35.0 39.0 29.0 41.0 74-75 34.84348940366908 36.0 35.0 39.0 29.0 41.0 76-77 34.467820008302695 36.0 34.0 38.5 28.5 40.0 78-79 34.06840216226493 35.0 34.0 37.0 28.0 39.0 80-81 33.7367983091617 35.0 34.0 37.0 27.5 39.0 82-83 33.44175935408313 35.0 34.0 36.5 27.0 38.5 84-85 33.154254090636 35.0 34.0 36.0 27.0 37.0 86-87 32.9027181295027 35.0 34.0 36.0 26.0 37.0 88-89 32.691364456303546 35.0 34.0 35.0 26.0 36.5 90-91 32.519188281765786 35.0 33.0 35.0 26.0 36.0 92-93 32.377442443695045 35.0 33.0 35.0 25.0 36.0 94-95 32.25866083052183 35.0 33.0 35.0 25.0 36.0 96-97 32.13764944662326 35.0 33.0 35.0 25.0 35.5 98-99 32.01804536181697 35.0 33.0 35.0 25.0 35.0 100-101 30.426700139838744 33.5 30.0 34.5 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4858221584146847 1101 2 0.43231240294325346 1101 3 0.5555052153251125 1101 4 0.4629741203859794 1101 5 0.4260093787360191 1101 6 0.4050872777701926 1101 7 0.49337322856089827 1101 8 0.5740811259332048 1101 9 0.640140306011709 1101 10-11 0.7171293446129354 1101 12-13 0.731695526952457 1101 14-15 0.9128822670079444 1101 16-17 0.8207440474839984 1101 18-19 0.9200128934575886 1101 20-21 0.8375645058889063 1101 22-23 0.8563205531130009 1101 24-25 0.8805075540855114 1101 26-27 0.7470260253578687 1101 28-29 0.8063858921886649 1101 30-31 0.8854133262062618 1101 32-33 0.7932829418390028 1101 34-35 0.8188765561195979 1101 36-37 0.839830159940405 1101 38-39 0.8087250185736394 1101 40-41 0.7823596719431762 1101 42-43 0.8912615177522554 1101 44-45 0.931740766024518 1101 46-47 1.0586178490480975 1101 48-49 1.0169508847512674 1101 50-51 0.992674961578544 1101 52-53 1.0761691331701186 1101 54-55 1.0273306884130804 1101 56-57 1.118567044791476 1101 58-59 1.1474538917199766 1101 60-61 0.9617492921418958 1101 62-63 1.0292836241669931 1101 64-65 1.0635209653260986 1101 66-67 0.925914138331315 1101 68-69 1.0008482061962596 1101 70-71 1.0264679575454139 1101 72-73 0.9192881140216329 1101 74-75 0.8517021897546613 1101 76-77 0.7978083266751526 1101 78-79 0.9537886586094757 1101 80-81 0.8553472383644234 1101 82-83 0.7875569667755897 1101 84-85 0.8117546020635444 1101 86-87 0.8729171003152203 1101 88-89 0.8733957065949909 1101 90-91 0.8145932361728683 1101 92-93 0.8069784407838725 1101 94-95 0.9333204411026585 1101 96-97 1.0814089041419095 1101 98-99 1.0154567814544535 1101 100-101 1.0727906145808603 1102 1 0.23981831955136812 1102 2 0.3379725361088006 1102 3 0.46650750112132044 1102 4 0.24737057461430823 1102 5 0.3238802830192995 1102 6 0.2148913125588976 1102 7 0.3257066919802014 1102 8 0.35948707378200595 1102 9 0.6416883953409425 1102 10-11 0.6471293247018224 1102 12-13 0.6441732265016711 1102 14-15 0.8372483966054247 1102 16-17 0.6983110882141759 1102 18-19 0.6713145827167324 1102 20-21 0.8169614778062595 1102 22-23 0.9204517713547702 1102 24-25 0.8032907498993964 1102 26-27 0.8860115937804309 1102 28-29 0.8464833969275105 1102 30-31 0.9561644929977007 1102 32-33 0.8585809515237699 1102 34-35 0.8498343604809264 1102 36-37 0.9082128993914935 1102 38-39 0.8687769467661894 1102 40-41 0.8729393243747197 1102 42-43 0.8808106703351228 1102 44-45 0.9903297249120442 1102 46-47 1.0110133555072593 1102 48-49 0.958655614836168 1102 50-51 0.8392269534866728 1102 52-53 0.8553857103695108 1102 54-55 0.8005410772373764 1102 56-57 0.6621644260813966 1102 58-59 0.8655916248791584 1102 60-61 0.8360568830560524 1102 62-63 0.8634056036505768 1102 64-65 0.9096135082146404 1102 66-67 0.6967232464725797 1102 68-69 0.7915017288710473 1102 70-71 0.7614938021749893 1102 72-73 0.618893575242744 1102 74-75 0.620961715232923 1102 76-77 0.6414926199898048 1102 78-79 0.6990448350379026 1102 80-81 0.5318971196941717 1102 82-83 0.5890346703070222 1102 84-85 0.6390296462662235 1102 86-87 0.7402423603544079 1102 88-89 0.6627201622250567 1102 90-91 0.6242285242469094 1102 92-93 0.7366384385538325 1102 94-95 0.8440389934848227 1102 96-97 0.8180585003444634 1102 98-99 0.8423161810944535 1102 100-101 0.7240757776130842 1103 1 0.27244626068926436 1103 2 0.2796104580981549 1103 3 0.44845245561073455 1103 4 0.31516101608008995 1103 5 0.2402972098089009 1103 6 0.291913385847991 1103 7 0.42536929723161876 1103 8 0.35076530477117274 1103 9 0.4347349158141469 1103 10-11 0.38608694215060524 1103 12-13 0.4312522208095757 1103 14-15 0.4723759770686584 1103 16-17 0.3939402031841439 1103 18-19 0.40318986141988233 1103 20-21 0.44316318019065193 1103 22-23 0.4642811197796064 1103 24-25 0.38095629956757904 1103 26-27 0.47708850273303227 1103 28-29 0.45090772738419105 1103 30-31 0.5195596219224967 1103 32-33 0.5119504725478308 1103 34-35 0.37794731066847476 1103 36-37 0.437584932022709 1103 38-39 0.4867306754863847 1103 40-41 0.373490172943832 1103 42-43 0.4935731431205852 1103 44-45 0.6040233940881308 1103 46-47 0.47875756503956524 1103 48-49 0.46483216836028873 1103 50-51 0.5160444258786612 1103 52-53 0.4898862087563103 1103 54-55 0.5477966709734332 1103 56-57 0.553771854667815 1103 58-59 0.6353180695451002 1103 60-61 0.5237150880766492 1103 62-63 0.7172249843394027 1103 64-65 0.6687787431122061 1103 66-67 0.5476695256216502 1103 68-69 0.5369719830483106 1103 70-71 0.45500723238772167 1103 72-73 0.4221676814253641 1103 74-75 0.5611397245711203 1103 76-77 0.3842422396419778 1103 78-79 0.5034230975041254 1103 80-81 0.4938459432390161 1103 82-83 0.5146578177508232 1103 84-85 0.5637601239306207 1103 86-87 0.4861814915875229 1103 88-89 0.551035614838959 1103 90-91 0.6008018854724142 1103 92-93 0.3944008129734726 1103 94-95 0.34110113444959467 1103 96-97 0.3104876113797843 1103 98-99 0.4216342764699519 1103 100-101 0.5034273173659862 1104 1 0.11006589922059362 1104 2 0.17969298790388422 1104 3 0.3062236164904206 1104 4 0.22853737574379096 1104 5 0.1289175964521334 1104 6 0.1262719095738234 1104 7 0.14783759153647225 1104 8 0.1919548027049629 1104 9 0.48498458331996375 1104 10-11 0.3544079652827037 1104 12-13 0.3617067581676636 1104 14-15 0.47886926187367607 1104 16-17 0.43766541455544683 1104 18-19 0.6097474185376299 1104 20-21 0.5587991643734256 1104 22-23 0.5261836692974882 1104 24-25 0.5638986058897117 1104 26-27 0.6513844759910015 1104 28-29 0.5652270939827773 1104 30-31 0.5299908846879049 1104 32-33 0.6234748099344216 1104 34-35 0.6744423173183662 1104 36-37 0.6555593596577296 1104 38-39 0.6260316361570659 1104 40-41 0.59024595003023 1104 42-43 0.3552585323762685 1104 44-45 0.41858960792939115 1104 46-47 0.5069542590974194 1104 48-49 0.5534708124965206 1104 50-51 0.47060553355927937 1104 52-53 0.44246719483904684 1104 54-55 0.5689456758658551 1104 56-57 0.5517620460894648 1104 58-59 0.593417865298683 1104 60-61 0.6319486236772676 1104 62-63 0.667963893283499 1104 64-65 0.6995930866736941 1104 66-67 0.6391143315875425 1104 68-69 0.4919605832478027 1104 70-71 0.6145784633286766 1104 72-73 0.4828256042867096 1104 74-75 0.4509208721510447 1104 76-77 0.5178477531079935 1104 78-79 0.5281530944166875 1104 80-81 0.568088628367029 1104 82-83 0.5233895008838658 1104 84-85 0.6766582263388301 1104 86-87 0.6478285846383898 1104 88-89 0.6604952227333172 1104 90-91 0.6997062874578788 1104 92-93 0.7085462138664553 1104 94-95 0.6915455485473458 1104 96-97 0.5637977097030671 1104 98-99 0.5588979681053488 1104 100-101 0.628611376644951 1105 1 0.2508879712926628 1105 2 0.2041716332158643 1105 3 0.2051760411372925 1105 4 0.19207452400316782 1105 5 0.1952756211434945 1105 6 0.20425544866847645 1105 7 0.30518660721882185 1105 8 0.18115650440666542 1105 9 0.4310341050639295 1105 10-11 0.28267651160680174 1105 12-13 0.2955513666650518 1105 14-15 0.33945380474988696 1105 16-17 0.29544924004760986 1105 18-19 0.3027772539285749 1105 20-21 0.3027677857726019 1105 22-23 0.2865575848797377 1105 24-25 0.19080572133849927 1105 26-27 0.20664146909521008 1105 28-29 0.3470120038926865 1105 30-31 0.2706867889254738 1105 32-33 0.20397857759096638 1105 34-35 0.3564740017806045 1105 36-37 0.37707844819070147 1105 38-39 0.3193266672993218 1105 40-41 0.41562097783581464 1105 42-43 0.3609489450111312 1105 44-45 0.3578858486145222 1105 46-47 0.42710496535923426 1105 48-49 0.5351132187917003 1105 50-51 0.4684227952654254 1105 52-53 0.3805983815813434 1105 54-55 0.4746013315215052 1105 56-57 0.4157913531743276 1105 58-59 0.5076126156045433 1105 60-61 0.37024282065203096 1105 62-63 0.5100316554901525 1105 64-65 0.5010525322303678 1105 66-67 0.569754694172353 1105 68-69 0.6257249031232348 1105 70-71 0.5487143353117787 1105 72-73 0.5022457188457139 1105 74-75 0.41163297314091807 1105 76-77 0.5437886245349759 1105 78-79 0.5033619838616872 1105 80-81 0.5132511520017644 1105 82-83 0.6345996693857003 1105 84-85 0.48916170203675335 1105 86-87 0.3609451734494229 1105 88-89 0.4502700670220605 1105 90-91 0.5346436553813589 1105 92-93 0.4632415341867784 1105 94-95 0.5716847154920757 1105 96-97 0.4828042162095727 1105 98-99 0.5669296108592121 1105 100-101 0.6086747733750144 1106 1 0.24837224802222124 1106 2 0.24104561411500214 1106 3 0.11417130474471548 1106 4 0.12071611108290625 1106 5 0.09196791091753909 1106 6 0.16479392215244104 1106 7 0.1309669473073356 1106 8 0.060803604336783224 1106 9 0.0348581597265607 1106 10-11 0.00876161138163667 1106 12-13 0.1680104085161318 1106 14-15 0.1554187476802369 1106 16-17 0.12799932157567895 1106 18-19 -0.010339059449790966 1106 20-21 0.21318202600864566 1106 22-23 0.052676848797744924 1106 24-25 0.20861378097045957 1106 26-27 0.25070933150689 1106 28-29 0.1430811862826218 1106 30-31 0.05425305959866478 1106 32-33 0.24525313306887853 1106 34-35 0.0982561665449424 1106 36-37 0.2399152367612487 1106 38-39 0.313534292114916 1106 40-41 0.3584950363807309 1106 42-43 0.45589808702526113 1106 44-45 0.4066590452912777 1106 46-47 0.40442366246305284 1106 48-49 0.56240279872803 1106 50-51 0.4955502896361139 1106 52-53 0.46677452103316597 1106 54-55 0.5467099967905469 1106 56-57 0.49836666958087505 1106 58-59 0.6097041988019924 1106 60-61 0.5524425255980603 1106 62-63 0.532791574738539 1106 64-65 0.49274443377552046 1106 66-67 0.5160053735729235 1106 68-69 0.3606606512403303 1106 70-71 0.49957814461374284 1106 72-73 0.6268050790444875 1106 74-75 0.5075458933986994 1106 76-77 0.5750119337981161 1106 78-79 0.5190192553889759 1106 80-81 0.3435698879284743 1106 82-83 0.4635799861804841 1106 84-85 0.48560455474270725 1106 86-87 0.4802217088947245 1106 88-89 0.4594206221893842 1106 90-91 0.44392426723010203 1106 92-93 0.23764754397014798 1106 94-95 0.2969923868951341 1106 96-97 0.3323150477958805 1106 98-99 0.2687717569885706 1106 100-101 0.22001882182597754 1107 1 0.2205147409194339 1107 2 0.18254047825137576 1107 3 0.23655662807343703 1107 4 0.2150721247785441 1107 5 0.25449674703128267 1107 6 0.3253066586085751 1107 7 0.21743218613106308 1107 8 0.2655551888053438 1107 9 0.34678477637015703 1107 10-11 0.22897360234833997 1107 12-13 0.21811361144952457 1107 14-15 0.3790743777037946 1107 16-17 0.38282709409212856 1107 18-19 0.3395012795414871 1107 20-21 0.3384685659178217 1107 22-23 0.30778357277239365 1107 24-25 0.3980081618742801 1107 26-27 0.37243901267053303 1107 28-29 0.3660596229403055 1107 30-31 0.31188036345238146 1107 32-33 0.45464035047496765 1107 34-35 0.48316577291681995 1107 36-37 0.6407548229781952 1107 38-39 0.49727922302965766 1107 40-41 0.6097802164394963 1107 42-43 0.6456542852720304 1107 44-45 0.6122672141069927 1107 46-47 0.7122631394062964 1107 48-49 0.6564027623034647 1107 50-51 0.7222464602002105 1107 52-53 0.5953146851865441 1107 54-55 0.5540808110009934 1107 56-57 0.5484319302593406 1107 58-59 0.6023946992755143 1107 60-61 0.5195572144108596 1107 62-63 0.6127756693452682 1107 64-65 0.8398802488358612 1107 66-67 0.776710856684069 1107 68-69 0.5795088457960631 1107 70-71 0.6163279807031969 1107 72-73 0.6252242529353538 1107 74-75 0.5370695207996903 1107 76-77 0.5846310542662962 1107 78-79 0.4358389052274916 1107 80-81 0.5882865048148957 1107 82-83 0.6852741533935145 1107 84-85 0.7436225120531148 1107 86-87 0.7219839900437961 1107 88-89 0.7589628868259837 1107 90-91 0.8905726001991923 1107 92-93 0.7749675459127943 1107 94-95 0.7027665910183956 1107 96-97 0.7095023429076974 1107 98-99 0.6699500916574763 1107 100-101 0.8206963187298939 1108 1 0.2991053734367668 1108 2 0.21965867348475143 1108 3 0.23243687630970555 1108 4 0.03705761063837798 1108 5 0.27504699061817917 1108 6 0.18180668893059249 1108 7 0.18560619409564794 1108 8 0.2556904250138814 1108 9 0.2346377752179194 1108 10-11 0.32407962834442117 1108 12-13 0.4199026106207171 1108 14-15 0.448164110667868 1108 16-17 0.3720930990649336 1108 18-19 0.38552808420703855 1108 20-21 0.43722334163595633 1108 22-23 0.40907529081803773 1108 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>>Per sequence quality scores pass #Quality Count 6 25.0 7 472.0 8 945.0 9 846.0 10 949.0 11 1330.0 12 1839.0 13 2263.0 14 2653.0 15 2777.0 16 3154.0 17 3708.0 18 4350.0 19 5084.0 20 6260.0 21 7400.0 22 8408.0 23 8495.0 24 8496.0 25 8709.0 26 9081.0 27 10199.0 28 11459.0 29 13691.0 30 16254.0 31 20262.0 32 25252.0 33 33856.0 34 48589.0 35 75997.0 36 140144.0 37 296560.0 38 430850.0 39 136061.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75411774229123 19.691196248786717 13.720312115916885 23.834373893005168 2 14.515974515632902 21.167634942902275 41.17601190003201 23.140378641432815 3 17.394439571913725 27.093738001754108 30.949886042192237 24.561936384139923 4 11.9612996462815 18.088137768268712 40.28298965282414 29.667572932625646 5 12.78443965360391 38.67176215382856 35.874541699497456 12.669256493070073 6 29.108069430715954 39.06981614509959 19.176102501873306 12.646011922311148 7 25.91480010783104 33.44581824230943 23.8247938622451 16.814587787614432 8 24.85624384074573 36.06903860306814 21.522467400046487 17.55225015613965 9 26.313076890515102 16.116656554411975 20.68610843555127 36.88415811952165 10-11 23.60578990324169 26.621123150924102 30.284927938117455 19.488159007716753 12-13 24.83801207677986 24.4477557849647 29.457517762045406 21.256714376210038 14-15 22.149996546733096 25.76367326599587 26.910751987856386 25.175578199414655 16-17 21.80051108350148 28.784353507590875 27.36220165444903 22.05293375445861 18-19 21.890481682461342 28.318719454368978 28.736750409007904 21.05404845416178 20-21 22.763526966301313 27.52023503008798 28.444596602728005 21.2716414008827 22-23 22.468922454478143 27.512957177262503 28.10866428378873 21.909456084470623 24-25 22.203466485859057 27.56787526057312 28.26955681589483 21.959101437672988 26-27 22.06667255826181 27.88487030940529 28.120732151994243 21.92772498033866 28-29 22.191844200479597 27.72215831409282 28.270745036764293 21.815252448663287 30-31 22.488379571215646 27.63371012312196 28.167072765903406 21.710837539758984 32-33 22.251515167268078 27.786433636750484 28.15196008171989 21.810091114261546 34-35 22.416900659536847 27.788810078489412 28.072720602487394 21.721568659486348 36-37 22.421059432579977 27.722752424527553 28.022703930263315 21.833484212629152 38-39 22.27141786683163 27.895972748154357 28.04966169123932 21.782947693774688 40-41 22.412593358885033 27.7548715199053 28.05827629254295 21.77425882866672 42-43 22.3115203211761 27.66679464795615 28.002169988362862 22.01951504250489 44-45 22.370597177529852 27.845918944028114 27.83893814642 21.944545732022032 46-47 22.3915440104831 27.69603174337946 27.764725889932667 22.14769835620477 48-49 22.26583995954096 27.840914576320024 27.794648088778185 22.098597375360832 50-51 22.297855781177244 27.863890784678784 27.722678160723213 22.115575273420763 52-53 22.38247938622451 27.890662886143936 27.632559034154667 22.09429869347689 54-55 22.250289814496444 27.801100738107955 27.737716581102383 22.210892866293218 56-57 22.302385873242084 27.920888254510967 27.611802300841187 22.16492357140576 58-59 22.296667560307778 27.84892662810395 27.715288912191223 22.139116899397052 60-61 22.30220021373123 27.8176986983783 27.739201857189215 22.14089923070125 62-63 22.159056730862776 27.82835555430132 27.82798423527961 22.18460347955629 64-65 22.192846761838208 27.66337851295643 27.86192279386373 22.281851931341627 66-67 22.10450996657386 27.742655124091105 27.706748574691936 22.446086334643102 68-69 22.094521484889913 27.660407960782766 27.775256934197074 22.469813620130243 70-71 22.11739473662713 27.33988538124438 27.86715839206981 22.675561490058676 72-73 22.000837695712974 27.575153113398603 27.804034158379444 22.61997503250898 74-75 22.054047711523737 27.48852438563411 27.78353734838116 22.67389055446099 76-77 21.966342158596294 27.434460336073418 27.878038039405862 22.721159465924426 78-79 22.002248707995463 27.4720006891681 27.70047328322507 22.825277319611363 80-81 21.913614857513753 27.591454018451582 27.663081457739068 22.831849666295597 82-83 22.030914536471325 27.463274692157963 27.777819035446853 22.72799173592385 84-85 22.01082617739692 27.352695887493304 27.6851749395307 22.951302995579077 86-87 21.92274930544777 27.33910561129879 27.78821596805468 22.949929115198756 88-89 22.041608524296517 27.216978785059016 27.762929142676363 22.978483547968107 90-91 22.00154320185422 27.16425148397647 27.89549003342614 22.93871528074317 92-93 22.072576530706968 27.098825072351513 27.8466987139737 22.981899682967818 94-95 21.989363937942194 27.046877541214553 27.837230078920143 23.126528441923107 96-97 21.965153937726832 26.924305132148728 27.968231429778744 23.142309500345696 98-99 22.01825255783108 26.86170074508875 27.857095646581527 23.262951050498646 100-101 22.120810871626844 26.717220513742145 27.85093175082117 23.311036863809836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 24.0 1 20.0 2 26.5 3 44.0 4 44.0 5 45.5 6 60.5 7 123.5 8 250.5 9 322.0 10 319.0 11 332.0 12 383.5 13 442.5 14 496.5 15 578.0 16 643.5 17 692.5 18 802.0 19 909.0 20 1050.5 21 1312.0 22 1633.5 23 2134.0 24 2769.5 25 3666.5 26 5181.0 27 7078.5 28 9410.5 29 12443.0 30 15594.5 31 19145.0 32 22947.0 33 26608.5 34 30295.5 35 33725.5 36 37907.5 37 41721.5 38 44018.0 39 46211.0 40 47886.5 41 49319.0 42 50569.0 43 52013.0 44 53946.5 45 55307.0 46 55841.5 47 55625.5 48 55822.0 49 55681.0 50 53788.0 51 51056.0 52 48260.0 53 45375.5 54 42039.5 55 37369.5 56 32164.0 57 27446.5 58 23361.5 59 19669.5 60 15924.0 61 12537.5 62 9547.0 63 6907.5 64 4960.5 65 3533.5 66 2473.5 67 1764.5 68 1298.5 69 949.0 70 642.0 71 501.5 72 395.0 73 281.0 74 227.0 75 159.5 76 106.5 77 78.5 78 63.0 79 49.0 80 32.5 81 27.0 82 23.0 83 20.0 84 19.0 85 16.0 86 12.5 87 14.0 88 12.5 89 9.5 90 8.5 91 6.5 92 3.5 93 1.5 94 1.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.856595108540263E-4 48-49 2.9705521736644213E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1346551.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.546330730183755 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97418221704662 46.26813666442276 2 14.802686875490426 18.517074980195492 3 5.3810812414092135 10.097006610335052 4 2.3353049533249246 5.842590238659883 5 1.1844737342781173 3.704224296268746 6 0.7081561036685373 2.657553952119038 7 0.4297283266052115 1.881455102798459 8 0.27917455008013153 1.3969074992609722 9 0.18143086720091633 1.0213051522151304 >10 0.6978504543542392 6.952968608064062 >50 0.02019011636989673 0.8623484339743516 >100 0.005381785384775203 0.49473983263211435 >500 1.1959159564785277E-4 0.06133612705031623 >1k 2.3918319129570553E-4 0.24235250200368852 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 0.13835346748842042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1377 0.10226125857839771 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7131902170805266E-5 0.0 12-13 0.0 0.0 0.0 1.4852760868322106E-4 0.0 14-15 0.0 0.0 0.0 2.2279141302483158E-4 0.0 16-17 0.0 0.0 0.0 2.2279141302483158E-4 0.0 18-19 0.0 0.0 0.0 2.2279141302483158E-4 0.0 20-21 0.0 0.0 0.0 2.5992331519563686E-4 0.0 22-23 0.0 0.0 0.0 4.8271472822046844E-4 0.0 24-25 0.0 0.0 0.0 8.911656520993263E-4 0.0 26-27 0.0 0.0 0.0 0.0012996165759781844 0.0 28-29 0.0 0.0 0.0 0.005235598206083542 0.0 30-31 0.0 0.0 0.0 0.015149816085688548 0.0 32-33 0.0 0.0 0.0 0.03858004635546667 0.0 34-35 0.0 0.0 0.0 0.06758006195086558 0.0 36-37 0.0 0.0 0.0 0.11002182613209599 0.0 38-39 0.0 0.0 0.0 0.16549688797527906 0.0 40-41 0.0 0.0 0.0 0.22319986394871044 0.0 42-43 0.0 0.0 0.0 0.30715509475690117 0.0 44-45 0.0 0.0 0.0 0.393189712086657 0.0 46-47 0.0 0.0 0.0 0.5005380412624549 0.0 48-49 0.0 0.0 0.0 0.6597967696730387 7.426380434161053E-5 50-51 0.0 0.0 0.0 0.809623994932238 7.426380434161053E-5 52-53 0.0 0.0 0.0 0.9585971864415088 1.1139570651241579E-4 54-55 0.0 0.0 0.0 1.126916098981769 1.4852760868322106E-4 56-57 0.0 0.0 0.0 1.319519275541736 1.4852760868322106E-4 58-59 0.0 0.0 0.0 1.5324336025891334 1.4852760868322106E-4 60-61 0.0 0.0 0.0 1.763394034091542 1.4852760868322106E-4 62-63 0.0 0.0 0.0 2.0154082541247975 2.2279141302483158E-4 64-65 0.0 0.0 0.0 2.28528291910221 2.2279141302483158E-4 66-67 0.0 0.0 0.0 2.604877201086331 2.2279141302483158E-4 68-69 0.0 0.0 0.0 2.9614548576325737 2.2279141302483158E-4 70-71 0.0 0.0 0.0 3.3323654284167477 2.2279141302483158E-4 72-73 0.0 0.0 0.0 3.7176089134388524 2.2279141302483158E-4 74-75 0.0 0.0 0.0 4.087888241886122 2.2279141302483158E-4 76-77 0.0 0.0 0.0 4.472908935495202 2.2279141302483158E-4 78-79 0.0 0.0 0.0 4.8997772828507795 2.2279141302483158E-4 80-81 0.0 0.0 0.0 5.357353713301613 2.5992331519563686E-4 82-83 0.0 0.0 0.0 5.829708640816427 2.9705521736644213E-4 84-85 0.0 0.0 0.0 6.3025834149616315 2.9705521736644213E-4 86-87 0.0 0.0 0.0 6.794729646333485 2.9705521736644213E-4 88-89 0.0 0.0 0.0 7.327943761506248 2.9705521736644213E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1380 0.0 48.532608 1 GTATCAA 2535 0.0 44.59566 1 TCAACGC 2860 0.0 37.867134 4 CAACGCA 3030 0.0 35.89934 5 ATCAACG 3020 0.0 35.860928 3 AACGCAG 3195 0.0 34.78873 6 TATCAAC 3390 0.0 31.946901 2 ACGCAGA 3575 0.0 30.958042 7 CGCAGAG 3605 0.0 30.568655 8 GTACATG 3770 0.0 28.22281 1 TACATGG 3810 0.0 27.677166 2 ACATGGG 3800 0.0 26.625 3 TATACCG 90 8.9483487E-4 26.388887 5 CATGGGG 2610 0.0 24.02299 4 GCAGAGT 4535 0.0 23.880926 9 AGAGTAC 3815 0.0 21.851244 10-11 CAGAGTA 4150 0.0 20.087349 10-11 GGACCGT 120 0.004763917 19.791668 6 GAGTACT 2485 0.0 19.496983 12-13 AGTACTT 2805 0.0 18.966133 12-13 >>END_MODULE