FastQCFastQC Report
Fri 10 Feb 2017
SRR2031511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346551
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT23180.1721434984638532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.16130098302997806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.13218957172806675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.048.444481
GTATCAA25050.043.8521541
TCAACGC28000.037.6600954
ATCAACG29000.036.525263
CAACGCA29550.035.6846925
AACGCAG30450.034.6299746
TATCAAC32600.032.7832072
ACGCAGA34150.030.877977
CGCAGAG34900.030.2144058
GTACATG31500.025.8150061
TACATGG31000.024.8221722
GCAGAGT42400.024.3097429
ACATGGG31700.022.9254913
GGACCGA1252.2564334E-422.7996256
AGAGTAC36800.021.49081610-11
GAGTACT26400.020.69094812-13
CATGGGG20400.020.0241814
CAGAGTA41550.019.49125710-11
GAGTACA22700.018.8539921
GTACTTT30150.017.56604614-15
Produced by FastQC (version 0.11.5)