SRR2031509_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2031509_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40550
Sequences flagged as poor quality	0
Sequence length	101
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1365	3.3662145499383476	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	2.9519112207151665	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694	1.7114673242909988	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	419	1.033292231812577	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.48828606658446366	No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	187	0.46115906288532676	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	148	0.3649815043156597	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	137	0.33785450061652283	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA	122	0.3008631319358816	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113	0.2786683107274969	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	108	0.26633785450061653	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.2638717632552405	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA	100	0.2466091245376079	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.22441430332922319	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	89	0.21948212083847102	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA	87	0.21454993834771888	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.20715166461159062	No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA	83	0.20468557336621457	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	82	0.20221948212083848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	75	0.18495684340320592	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	74	0.18249075215782984	No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73	0.18002466091245375	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71	0.17509247842170161	No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	66	0.16276202219482122	No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.16029593094944514	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	64	0.15782983970406905	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	61	0.1504315659679408	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	60	0.14796547472256474	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	58	0.14303329223181258	No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	55	0.13563501849568432	No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.13316892725030827	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	53	0.13070283600493218	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	53	0.13070283600493218	No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA	50	0.12330456226880394	No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	48	0.11837237977805179	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	46	0.11344019728729964	No Hit
GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.11097410604192356	No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	44	0.10850801479654747	No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.1060419235511714	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	41	0.10110974106041924	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATGG	40	7.415514E-6	59.374996	2
ATATGGG	40	7.415514E-6	59.374996	3
CTGCTTA	25	0.0049865046	57.0	7
GTACCCA	25	0.0049865046	57.0	1
TATGGGA	35	2.7559118E-4	54.28571	4
GTATATG	45	1.4899537E-5	52.777775	1
TGGGGTG	60	8.150017E-5	39.583336	6
ATGGGGC	110	1.538865E-9	38.863636	5
TGGGGCA	115	2.3774192E-9	37.173912	6
GGGTGGT	65	1.3057205E-4	36.538464	8
ATGGGGT	70	2.0187302E-4	33.92857	5
GGGGCAG	130	7.876224E-9	32.884617	7
GGTGGTA	75	3.0267605E-4	31.666668	9
GGGGTGG	75	3.0267605E-4	31.666668	7
GGGCAGT	135	1.1379598E-8	31.666666	8
GAGTGCA	40	4.4573148E-4	29.687498	12-13
AGAGTGC	50	4.8973387E-5	28.5	10-11
CATGGGG	205	2.910383E-11	27.804876	4
AACGCAG	1495	0.0	27.324415	6
CAGAGTG	55	9.370591E-5	25.909092	10-11

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content