FastQCFastQC Report
Fri 10 Feb 2017
SRR2031509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40550
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT556813.731196054254008No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT15183.743526510480888TruSeq Adapter, Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14763.6399506781750928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14143.487053020961776No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT10152.5030826140567197RNA PCR Primer, Index 10 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8051.9852034525277433No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG5911.4574599260172627TruSeq Adapter, Index 10 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4111.0135635018495686No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT3630.8951911220715166TruSeq Adapter, Index 10 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC2200.5425400739827373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.5129469790382244No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1850.45622688039457465No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1840.45376078914919854TruSeq Adapter, Index 10 (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1460.3600493218249075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.3526510480887793No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA1380.3403205918618989RNA PCR Primer, Index 10 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1270.313193588162762No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC1180.2909987669543773Illumina Paired End PCR Primer 2 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1100.27127003699136865No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1100.27127003699136865No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1080.26633785450061653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.2638717632552405No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA1010.24907521578298397No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA970.23921085080147964No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT970.23921085080147964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG960.23674475955610358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.23674475955610358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG950.23427866831072752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC890.21948212083847102No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.21948212083847102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC830.20468557336621457No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC820.20221948212083848No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA780.19235511713933415No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1898890258939581No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT730.18002466091245375No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA730.18002466091245375No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA690.17016029593094945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT660.16276202219482122No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG660.16276202219482122No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA650.16029593094944514No Hit
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC650.16029593094944514No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA600.14796547472256474No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG570.1405672009864365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC570.1405672009864365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC530.13070283600493218No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA510.12577065351418001No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT510.12577065351418001No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT500.12330456226880394No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA490.12083847102342786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG490.12083847102342786No Hit
GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11837237977805179No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.1159062885326757No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.11344019728729964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT450.11097410604192356No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.11097410604192356No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG440.10850801479654747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC440.10850801479654747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT410.10110974106041924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG156.571918E-494.998775
GCGGCCT156.571918E-494.998779
TGGGCGG156.571918E-494.998776
GGGCGGC250.00498676156.9992567
CTTATAC10800.051.5211941
TATACAC12050.048.0906643
GATATCG205.3587736E-447.49938636-37
CTGATAT205.3587736E-447.49938634-35
GAGTATA252.8038487E-547.49938212-13
TTATACA12200.046.7207032
ATACACA13800.041.992214
TACACAT13900.041.6901055
ATCGTAT308.223369E-539.5828238-39
CACATCT15000.038.632837
ATGGGAG250.001607076637.99950418-19
TATCGTA250.001607076637.99950438-39
TGATATC250.001607076637.99950434-35
ATATCGT250.001607076637.99950436-37
ACATCTC15400.037.629388
ACACATC15450.037.5076076
Produced by FastQC (version 0.11.5)