##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031509_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40550 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.434401972873 33.0 31.0 34.0 31.0 34.0 2 32.503057953144264 34.0 31.0 34.0 31.0 34.0 3 32.457139334155364 34.0 31.0 34.0 30.0 34.0 4 36.07982737361282 37.0 35.0 37.0 35.0 37.0 5 36.04271270036991 37.0 35.0 37.0 35.0 37.0 6 36.213662145499384 37.0 37.0 37.0 35.0 37.0 7 36.18350184956844 37.0 36.0 37.0 35.0 37.0 8 36.2226140567201 37.0 37.0 37.0 35.0 37.0 9 37.97383477188656 39.0 39.0 39.0 35.0 39.0 10-11 37.96217016029593 39.0 38.0 39.0 35.0 39.0 12-13 37.9050924784217 39.0 38.0 39.0 35.0 39.0 14-15 38.94863131935882 40.5 38.0 41.0 35.5 41.0 16-17 38.84155363748459 40.0 38.0 41.0 35.0 41.0 18-19 39.19145499383477 40.5 39.0 41.0 36.0 41.0 20-21 39.410591861898894 41.0 39.0 41.0 36.0 41.0 22-23 39.33928483353884 41.0 39.0 41.0 35.0 41.0 24-25 39.1944512946979 41.0 38.5 41.0 35.0 41.0 26-27 38.77824907521578 40.0 38.0 41.0 35.0 41.0 28-29 38.34702836004932 40.0 37.0 41.0 35.0 41.0 30-31 37.54880394574599 39.0 35.5 41.0 34.5 41.0 32-33 35.75088779284833 36.5 35.0 40.0 30.5 41.0 34-35 35.53414303329223 37.0 35.0 40.0 31.0 41.0 36-37 35.213341553637484 36.5 35.0 40.0 29.5 41.0 38-39 33.82911220715167 35.0 34.0 40.0 21.5 41.0 40-41 33.48615289765721 35.0 34.0 39.0 18.0 41.0 42-43 32.547928483353886 35.0 33.5 39.0 11.0 41.0 44-45 31.349506781750925 35.0 31.5 39.0 7.5 41.0 46-47 29.668606658446365 35.0 22.5 39.0 7.0 40.5 48-49 27.71765721331689 35.0 12.5 37.0 7.0 40.0 50-51 26.06016029593095 34.0 10.0 35.5 7.0 40.0 52-53 25.688002466091245 34.0 8.0 35.5 7.0 40.0 54-55 25.143316892725032 34.0 7.5 35.5 7.0 40.5 56-57 24.485795314426632 33.0 7.0 35.0 7.0 40.0 58-59 23.878014796547472 31.5 7.0 35.0 7.0 40.0 60-61 22.989716399506783 27.5 7.0 35.0 7.0 39.5 62-63 22.274254007398273 24.0 7.0 35.0 7.0 39.0 64-65 21.426078914919852 20.0 7.0 35.0 7.0 38.5 66-67 20.08689272503083 17.0 7.0 34.5 7.0 37.0 68-69 18.229371146732426 12.5 7.0 32.0 5.0 36.5 70-71 16.73951911220715 10.0 7.0 27.0 5.0 35.5 72-73 15.591356350184956 9.0 7.0 24.0 5.0 35.0 74-75 15.202219482120839 8.0 7.0 23.5 5.0 35.0 76-77 14.60921085080148 8.0 7.0 21.5 5.0 34.5 78-79 14.929950678175093 8.0 7.0 23.0 5.0 35.0 80-81 14.90288532675709 8.0 7.0 23.0 5.0 35.0 82-83 14.876535141800247 8.0 7.0 23.0 5.0 35.0 84-85 14.724204685573365 8.0 7.0 23.0 5.0 35.0 86-87 14.591911220715165 8.0 7.0 21.5 5.0 35.0 88-89 14.455795314426634 8.0 7.0 20.0 5.0 35.0 90-91 14.345462392108509 8.0 7.0 20.0 5.0 35.0 92-93 14.277558569667077 8.0 7.0 20.0 5.0 35.0 94-95 14.219593094944512 8.0 7.0 20.0 5.0 35.0 96-97 14.179432799013563 8.0 7.0 20.0 5.0 35.0 98-99 14.165191122071516 8.0 7.0 20.0 5.0 35.0 100-101 13.70596794081381 7.5 7.0 19.5 5.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9032786150088299 1101 2 0.46023012240055294 1101 3 0.3981192347724871 1101 4 0.5059467871891599 1101 5 0.4761054516977694 1101 6 0.25700363866057074 1101 7 0.5089368089019288 1101 8 0.5936894452548671 1101 9 -0.29242867724050825 1101 10-11 0.34009350187219667 1101 12-13 0.5009325849921638 1101 14-15 0.3552202921604177 1101 16-17 0.10711215134778485 1101 18-19 -0.06496390318719847 1101 20-21 0.4055584658267932 1101 22-23 0.29227866493693 1101 24-25 -0.12365543079317831 1101 26-27 0.04340079889882276 1101 28-29 -0.30516398665647415 1101 30-31 0.181773264627914 1101 32-33 0.21033974933789779 1101 34-35 0.7848004219813234 1101 36-37 0.6383556785015472 1101 38-39 -0.3319348971963052 1101 40-41 0.41350525562112495 1101 42-43 0.6143855060845169 1101 44-45 -1.015618307739782 1101 46-47 0.13206535296285438 1101 48-49 1.238482335761514 1101 50-51 1.3513206648784362 1101 52-53 2.2474812622351017 1101 54-55 3.719244959229581 1101 56-57 4.231652262192657 1101 58-59 2.081934090039791 1101 60-61 0.750245309861576 1101 62-63 0.8628058354971664 1101 64-65 -0.17904497600090252 1101 66-67 -0.6063110913513441 1101 68-69 -1.7605751741540416 1101 70-71 0.23204037858099014 1101 72-73 -0.7448920127242893 1101 74-75 0.056232079411971725 1101 76-77 -0.4092523262051131 1101 78-79 0.3092122766545504 1101 80-81 -0.3585102210375286 1101 82-83 -1.5035068430496654 1101 84-85 -1.6126922995372635 1101 86-87 -0.17422269082784503 1101 88-89 -1.5026376980584626 1101 90-91 -0.6524148095715248 1101 92-93 -1.3215100446025225 1101 94-95 -1.552730695847309 1101 96-97 -1.319629566188242 1101 98-99 -2.118464593360857 1101 100-101 -3.568115888573189 1102 1 1.0051304668606775 1102 2 1.0065264186968506 1102 3 0.7777488644021204 1102 4 0.6540949353373122 1102 5 0.5686980442903646 1102 6 0.7755221571790898 1102 7 0.3793071792723026 1102 8 0.32517092673634806 1102 9 0.8372009523891251 1102 10-11 0.7660194277981276 1102 12-13 0.7879696220292018 1102 14-15 0.9015165884567153 1102 16-17 0.7598899291255634 1102 18-19 0.763739800516511 1102 20-21 0.5027806880490218 1102 22-23 0.47283422049248713 1102 24-25 0.19578901365126455 1102 26-27 0.07580820630622753 1102 28-29 0.45409527260278537 1102 30-31 0.825291783146433 1102 32-33 1.2057101197082645 1102 34-35 0.4653559775368805 1102 36-37 1.0087260488719139 1102 38-39 0.5245465842851758 1102 40-41 1.3903571074729797 1102 42-43 1.401422543121555 1102 44-45 2.618640951519481 1102 46-47 2.956139427036927 1102 48-49 3.2245934468726283 1102 50-51 3.8605799241376957 1102 52-53 4.923407188161029 1102 54-55 5.3627634777481 1102 56-57 5.088133743674138 1102 58-59 5.605082238187936 1102 60-61 5.194689754306022 1102 62-63 3.3998428725342045 1102 64-65 1.043177246221319 1102 66-67 -1.2220518320920881 1102 68-69 -0.6587233223021904 1102 70-71 -1.1475892510486378 1102 72-73 -1.5550771979094744 1102 74-75 -0.8558049576250664 1102 76-77 -0.5388819558347429 1102 78-79 0.16569375813603138 1102 80-81 1.0211194085921012 1102 82-83 -1.5035068430496654 1102 84-85 -1.0154700773150402 1102 86-87 -0.4751486167537706 1102 88-89 -0.349859920280684 1102 90-91 -0.7172296243863396 1102 92-93 -1.2520656001580779 1102 94-95 -0.5203232884399007 1102 96-97 -0.24555549211416938 1102 98-99 0.8259798510835878 1102 100-101 0.6309581855008854 1103 1 0.5034964799325721 1103 2 0.3643695559517468 1103 3 0.5310168382583313 1103 4 0.08219951050070762 1103 5 0.3170640573622592 1103 6 0.5696398042379158 1103 7 0.3564313622788333 1103 8 0.09477876987359934 1103 9 0.003867619055789362 1103 10-11 0.10588870884388513 1103 12-13 0.17032256320567285 1103 14-15 0.014261686495935066 1103 16-17 0.36038012520399576 1103 18-19 0.535798624045924 1103 20-21 0.2846434331470604 1103 22-23 0.29064467800882454 1103 24-25 0.6328805169192364 1103 26-27 0.3086513435611309 1103 28-29 0.6272978869818715 1103 30-31 -0.4680088704483367 1103 32-33 0.10358593670172667 1103 34-35 0.47597689256955533 1103 36-37 0.652516898545116 1103 38-39 -0.4697344614664587 1103 40-41 -0.2313749186708094 1103 42-43 -2.1672049078588316 1103 44-45 -2.0847904210295454 1103 46-47 -1.7840566513944403 1103 48-49 -3.6013869452842364 1103 50-51 -4.007067134685833 1103 52-53 -3.905024184387994 1103 54-55 -4.307988156238828 1103 56-57 -5.607127694234361 1103 58-59 -5.81403540887089 1103 60-61 -4.816748154190709 1103 62-63 -4.423686539230502 1103 64-65 -3.7917900740401222 1103 66-67 -2.647705426863329 1103 68-69 -3.397285413805456 1103 70-71 -2.30608598307478 1103 72-73 -1.8189660867983637 1103 74-75 -1.8435500556642808 1103 76-77 -2.2709080996255917 1103 78-79 -1.4838160457855363 1103 80-81 -1.4216910489242363 1103 82-83 -2.2453369084091417 1103 84-85 -2.2907968747006624 1103 86-87 -2.1851159370152082 1103 88-89 -3.193814168646698 1103 90-91 -3.3087328923601955 1103 92-93 -2.9358891295698424 1103 94-95 -2.6796370139300976 1103 96-97 -1.8337907862318161 1103 98-99 -1.567810998589616 1103 100-101 -2.118224821035062 1104 1 0.10047581220602098 1104 2 0.34211200428243416 1104 3 0.44516628181953877 1104 4 0.3087495899919688 1104 5 0.30593528152760285 1104 6 0.5863329679899039 1104 7 0.33576363572875323 1104 8 0.579675431240851 1104 9 0.3011649163530876 1104 10-11 0.5693227311014297 1104 12-13 0.524456108515686 1104 14-15 1.0118769488170827 1104 16-17 1.0110160552516874 1104 18-19 0.827928989705704 1104 20-21 0.6773302375985679 1104 22-23 0.543029415687684 1104 24-25 0.481449674311925 1104 26-27 0.009366764864786603 1104 28-29 0.10424542275293902 1104 30-31 -0.16157007871542106 1104 32-33 1.2458752848734278 1104 34-35 0.7037193409002427 1104 36-37 -0.016799476653609702 1104 38-39 0.04894598368457537 1104 40-41 -0.6145227724069002 1104 42-43 -0.344072952373935 1104 44-45 0.2931905010690272 1104 46-47 0.6746078955053996 1104 48-49 0.5568006540798329 1104 50-51 -0.3083389947812236 1104 52-53 0.6418756566294981 1104 54-55 1.583108823093447 1104 56-57 0.9819025124429039 1104 58-59 0.8111633192690171 1104 60-61 0.4769720365883039 1104 62-63 0.5755185482098817 1104 64-65 0.17681087985495125 1104 66-67 0.7505457655055103 1104 68-69 1.0252106116317456 1104 70-71 0.9928011393417542 1104 72-73 2.018871751039475 1104 74-75 0.6858617090416015 1104 76-77 1.8109678940151053 1104 78-79 1.7276306950729694 1104 80-81 0.27862691609960955 1104 82-83 -0.09509843464125645 1104 84-85 0.31373412688916424 1104 86-87 1.3983498817447302 1104 88-89 0.7000650046442392 1104 90-91 -0.23599839315510707 1104 92-93 0.7715830484905712 1104 94-95 1.0393613962447823 1104 96-97 0.5517418051831289 1104 98-99 0.9731269982307342 1104 100-101 0.3197719993146979 1105 1 -1.151302281677335 1105 2 -1.2347016514785913 1105 3 -1.133069849048173 1105 4 -0.9394723161246787 1105 5 -0.8640504937213294 1105 6 -1.1981620533472253 1105 7 -0.8633828792072293 1105 8 -1.1528992487022478 1105 9 -1.0224481704178956 1105 10-11 -0.1170215078743908 1105 12-13 -0.6857145884971132 1105 14-15 -0.488834288736264 1105 16-17 -0.6458118252604024 1105 18-19 -0.4007338836940093 1105 20-21 -0.6178333470387045 1105 22-23 -0.9825751362326614 1105 24-25 0.0283913528325499 1105 26-27 -0.2246303901849913 1105 28-29 -0.5035070665785 1105 30-31 -1.2149128952161377 1105 32-33 -0.24238929549640886 1105 34-35 -0.8336206306502518 1105 36-37 -0.6067710271514741 1105 38-39 0.04574541469452953 1105 40-41 -0.013882658608892484 1105 42-43 0.278615525577699 1105 44-45 0.9453953374843849 1105 46-47 1.394735918264999 1105 48-49 2.6083653766971864 1105 50-51 2.900053608348223 1105 52-53 2.3225299951785736 1105 54-55 2.081330729210091 1105 56-57 0.5654729249606874 1105 58-59 0.5985032908195151 1105 60-61 1.1786078829609927 1105 62-63 2.531421819902622 1105 64-65 1.8823585327710255 1105 66-67 3.0798487527032385 1105 68-69 3.778411180621788 1105 70-71 4.152118351290547 1105 72-73 4.667876018464794 1105 74-75 3.1069143406205466 1105 76-77 3.3660888043991744 1105 78-79 3.549465687960593 1105 80-81 2.7594235021593523 1105 82-83 4.199709531219343 1105 84-85 3.033506530872094 1105 86-87 2.6337695119012015 1105 88-89 2.931572828257325 1105 90-91 2.8646417206429007 1105 92-93 2.7281975577366584 1105 94-95 3.398536360682904 1105 96-97 2.464970823675303 1105 98-99 2.50726640079119 1105 100-101 3.5644377176646262 1106 1 0.1043368160670255 1106 2 0.18509784726827405 1106 3 -0.5278066911534367 1106 4 0.11441239565477446 1106 5 -0.1297146541223313 1106 6 0.06123644289337449 1106 7 0.33962463958975775 1106 8 0.38469473626015827 1106 9 0.24196285715103016 1106 10-11 0.04776545954415923 1106 12-13 0.26416009821967634 1106 14-15 0.28246896940910204 1106 16-17 -0.7341576899220641 1106 18-19 -0.20649829472158387 1106 20-21 -0.0031716929033578367 1106 22-23 0.28434215700041676 1106 24-25 0.4795191723814227 1106 26-27 0.09366534916337343 1106 28-29 -0.6808253623178473 1106 30-31 -0.9862161533615037 1106 32-33 -0.6057978167996723 1106 34-35 0.022895660076564184 1106 36-37 -0.888099347953478 1106 38-39 -1.2631518284132355 1106 40-41 -1.6116270195111468 1106 42-43 -0.3604822187832042 1106 44-45 -0.9349304770519531 1106 46-47 -1.401003430105927 1106 48-49 -1.1980256007464227 1106 50-51 -0.8656105520527788 1106 52-53 -0.8861166213627811 1106 54-55 -1.3296968397122164 1106 56-57 0.026625807166201554 1106 58-59 -0.18658442847872792 1106 60-61 0.5081818177980857 1106 62-63 0.8998428725342045 1106 64-65 1.8090502620943312 1106 66-67 0.35929737425712105 1106 68-69 -0.7936439572228231 1106 70-71 -1.4293352827946695 1106 72-73 -1.128489896322174 1106 74-75 -0.42128114810125616 1106 76-77 0.1912767743239865 1106 78-79 0.6597413771836518 1106 80-81 0.4179448054175001 1106 82-83 -0.5471576367004598 1106 84-85 0.22460928776432532 1106 86-87 0.7252482086430536 1106 88-89 0.8664099209891578 1106 90-91 0.10023069307397847 1106 92-93 -0.4961132192056983 1106 94-95 0.17610528298866868 1106 96-97 1.2782540316953543 1106 98-99 1.5085195336232697 1106 100-101 0.9861169156596166 1107 1 -0.0920917553615439 1107 2 0.07319308536351343 1107 3 0.12219330884656188 1107 4 0.27631715755953223 1107 5 0.12147582206814178 1107 6 0.30052215717908837 1107 7 0.14319606816118835 1107 8 0.4168375934030095 1107 9 0.5788676190557922 1107 10-11 0.16324165002034619 1107 12-13 0.20046962202919616 1107 14-15 0.1598499217900482 1107 16-17 0.0015565957922305529 1107 18-19 -0.03626019948348613 1107 20-21 0.13611402138234752 1107 22-23 0.1450564427147114 1107 24-25 0.14023345809570742 1107 26-27 0.017474872972897515 1107 28-29 -0.2653491718416632 1107 30-31 -0.38859710574245554 1107 32-33 0.3918212308193816 1107 34-35 -0.4026995780186766 1107 36-37 -0.14821839557252758 1107 38-39 -1.73517563793704 1107 40-41 -1.5276984480825746 1107 42-43 -1.1194107902117736 1107 44-45 -0.5730257151471889 1107 46-47 -0.048027239629735874 1107 48-49 -0.9712398864607046 1107 50-51 -1.4019200758623072 1107 52-53 -1.505759478505638 1107 54-55 -0.5219587444741229 1107 56-57 -0.04242181188141814 1107 58-59 -1.6324177618120608 1107 60-61 -1.8483658012495354 1107 62-63 -1.200157127465797 1107 64-65 -0.7623783093342382 1107 66-67 -0.5484407209809739 1107 68-69 -1.1031677667466333 1107 70-71 -2.003144806604194 1107 72-73 -2.8814660867983637 1107 74-75 -2.589138290958399 1107 76-77 -2.9194375113902975 1107 78-79 -2.8426395751973015 1107 80-81 -3.3011028136301217 1107 82-83 -1.9923957319385561 1107 84-85 -2.6974145217594856 1107 86-87 -1.6057041723093253 1107 88-89 -1.1776376980584633 1107 90-91 -0.43667406883078463 1107 92-93 -1.3770656001580779 1107 94-95 0.20051004489343072 1107 96-97 -1.0580554921141694 1107 98-99 -0.4295757044719668 1107 100-101 0.8795692966119955 1108 1 0.08290824463845325 1108 2 0.19819308536351343 1108 3 -0.1528066911534367 1108 4 0.05131715755953081 1108 5 -0.07852417793185396 1108 6 0.02552215717908979 1108 7 -0.006803931838810229 1108 8 -0.13316240659698764 1108 9 0.003867619055789362 1108 10-11 0.2632416500203547 1108 12-13 0.212969622029199 1108 14-15 0.27234992179005246 1108 16-17 0.1390565957922263 1108 18-19 -0.2737601994834833 1108 20-21 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0.20330796517556493 2316 66-67 -0.19672503470646419 2316 68-69 0.607616546978857 2316 70-71 0.06646303653306163 2316 72-73 0.4163280308486943 2316 74-75 0.28390092472787565 2316 76-77 0.6114448415508793 2316 78-79 1.0063800326458345 2316 80-81 1.7547795393110537 2316 82-83 1.4213297582575226 2316 84-85 0.7925364586326715 2316 86-87 1.0403742590632223 2316 88-89 1.6150093607650664 2316 90-91 1.902051421365293 2316 92-93 1.240581458665451 2316 94-95 1.4429120056777442 2316 96-97 1.803464115728966 2316 98-99 0.20179684454764057 2316 100-101 -0.19665619358408293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 13.0 14 36.0 15 161.0 16 478.0 17 965.0 18 1428.0 19 1897.0 20 2738.0 21 3570.0 22 3309.0 23 1989.0 24 1741.0 25 2077.0 26 2291.0 27 2955.0 28 3920.0 29 2240.0 30 586.0 31 292.0 32 318.0 33 357.0 34 376.0 35 642.0 36 961.0 37 2388.0 38 2332.0 39 489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27435682188534 19.120043454644215 13.54501012295689 23.060589600513552 2 13.916152897657213 19.430332922318126 50.15536374845869 16.498150431565968 3 15.921085080147964 35.39580764488286 41.54870530209618 7.134401972872996 4 8.108508014796548 32.520345252774355 24.986436498150432 34.38471023427867 5 5.344019728729963 39.92355117139334 38.15536374845869 16.577065351418003 6 15.745992601726263 43.91368680641184 15.442663378545006 24.897657213316894 7 12.695437731196055 42.06905055487053 15.057953144266337 30.177558569667077 8 25.17632552404439 37.06288532675709 18.897657213316894 18.863131935881626 9 24.83600493218249 11.800246609124537 13.758323057953145 49.605425400739826 10-11 19.759556103575832 32.85819975339088 22.728729963008632 24.65351418002466 12-13 27.475955610357584 27.241676942046855 24.96177558569667 20.32059186189889 14-15 24.420468557336623 25.274969173859436 24.324290998766955 25.980271270036994 16-17 19.02712700369914 35.62515413070283 25.559802712700368 19.787916152897658 18-19 18.959309494451297 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11.918618988902589 44-45 8.336621454993836 34.416769420468555 43.1861898890259 14.060419235511715 46-47 16.235511713933416 26.92848335388409 35.31319358816276 21.52281134401973 48-49 15.504315659679408 25.526510480887794 34.473489519112206 24.495684340320594 50-51 10.048088779284834 25.870530209617755 39.81997533908755 24.261405672009865 52-53 9.908754623921084 27.21085080147965 45.45129469790382 17.429099876695435 54-55 9.146732429099877 27.551171393341555 36.51048088779285 26.79161528976572 56-57 16.556103575832307 29.468557336621455 26.865598027127003 27.109741060419235 58-59 8.039457459926016 32.17262638717632 38.80271270036991 20.98520345252774 60-61 14.400917397255206 38.83525074291915 24.841243418537836 21.922588441287807 62-63 7.256473489519112 45.02466091245376 25.278668310727497 22.44019728729963 64-65 7.763255240443896 44.32799013563502 25.569667077681874 22.339087546239213 66-67 8.193588162762023 43.28483353884094 25.704069050554875 22.81750924784217 68-69 8.293567121666111 41.711981652054895 26.717961997065316 23.276489229213677 70-71 8.876695437731195 39.69790382244143 27.31689272503083 24.10850801479655 72-73 9.933415536374845 36.4636251541307 27.766954377311958 25.836004932182487 74-75 10.31442663378545 33.41307028360049 28.04932182490752 28.223181257706536 76-77 10.748458692971639 31.727496917385945 29.491985203452526 28.03205918618989 78-79 11.623921085080148 30.795314426633784 28.864364981504316 28.716399506781755 80-81 11.781750924784216 29.992601726263874 28.353884093711468 29.871763255240445 82-83 12.681874229346485 29.78914919852034 27.16892725030826 30.360049321824906 84-85 13.467324290998766 29.958076448828606 25.488286066584465 31.08631319358816 86-87 14.260172626387178 29.38347718865598 24.006165228113442 32.3501849568434 88-89 15.907521578298397 28.05795314426634 22.577065351418003 33.457459926017265 90-91 16.694410535271707 26.95347661500142 22.249349560413815 34.10276328931306 92-93 17.274969173859432 25.57090012330456 22.034525277435264 35.11960542540074 94-95 18.26263871763255 25.110974106041922 21.31319358816276 35.31319358816276 96-97 19.356350184956845 25.059186189889026 20.643649815043155 34.940813810110974 98-99 19.526992034328835 25.180645638610077 20.3556191274754 34.936743199585685 100-101 20.350435887002305 24.516948421065614 20.616777025610673 34.51583866632141 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 82.0 1 52.5 2 22.0 3 20.5 4 14.0 5 11.5 6 17.5 7 29.0 8 49.5 9 58.0 10 43.0 11 32.0 12 33.5 13 31.0 14 35.5 15 47.5 16 47.0 17 53.5 18 68.5 19 92.5 20 115.5 21 139.0 22 174.5 23 221.5 24 296.5 25 360.5 26 432.0 27 563.5 28 731.5 29 931.0 30 1137.5 31 1293.0 32 1450.0 33 1624.5 34 1686.0 35 1725.5 36 1785.0 37 1830.0 38 1898.5 39 1911.0 40 1896.5 41 1802.5 42 1706.5 43 1635.0 44 1525.0 45 1472.5 46 1346.0 47 1103.0 48 992.0 49 981.0 50 989.0 51 983.0 52 941.0 53 884.0 54 600.0 55 265.5 56 121.0 57 65.0 58 44.0 59 33.0 60 22.5 61 14.0 62 8.5 63 4.5 64 0.5 65 0.5 66 0.5 67 0.5 68 0.5 69 1.0 70 2.0 71 2.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11837237977805179 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0012330456226880395 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0012330456226880395 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0012330456226880395 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.002466091245376079 100-101 0.0012330456226880395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 40550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.816276202219484 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81867506195995 30.490752157829842 2 8.39306048630183 6.180024660912454 3 3.0142675329894835 3.3292231812577064 4 1.4334516712438878 2.110974106041924 5 0.9176770044879095 1.6892725030826141 6 0.6095518788934289 1.3464858199753391 7 0.4889811775738495 1.2601726263871764 8 0.3952039654363989 1.1639950678175093 9 0.24783977493469087 0.8212083847102344 >10 1.366467948288566 9.805178791615289 >50 0.16076093509277245 4.401972872996301 >100 0.10717395672851499 6.853267570900123 >500 0.013396744591064373 3.4426633785450065 >1k 0.026793489182128746 13.373612823674478 >5k 0.006698372295532187 13.731196054254008 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 5568 13.731196054254008 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1518 3.743526510480888 TruSeq Adapter, Index 10 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 3.6399506781750928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1414 3.487053020961776 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 1015 2.5030826140567197 RNA PCR Primer, Index 10 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 805 1.9852034525277433 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 591 1.4574599260172627 TruSeq Adapter, Index 10 (96% over 28bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 411 1.0135635018495686 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 363 0.8951911220715166 TruSeq Adapter, Index 10 (96% over 26bp) CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC 220 0.5425400739827373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.5129469790382244 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.45622688039457465 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 184 0.45376078914919854 TruSeq Adapter, Index 10 (95% over 24bp) GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 146 0.3600493218249075 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 143 0.3526510480887793 No Hit CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 138 0.3403205918618989 RNA PCR Primer, Index 10 (96% over 28bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 127 0.313193588162762 No Hit ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC 118 0.2909987669543773 Illumina Paired End PCR Primer 2 (95% over 24bp) TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 110 0.27127003699136865 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 110 0.27127003699136865 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 108 0.26633785450061653 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.2638717632552405 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 101 0.24907521578298397 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 97 0.23921085080147964 No Hit TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT 97 0.23921085080147964 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 96 0.23674475955610358 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.23674475955610358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 95 0.23427866831072752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 89 0.21948212083847102 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.21948212083847102 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 83 0.20468557336621457 No Hit CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 82 0.20221948212083848 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.19235511713933415 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.1898890258939581 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 73 0.18002466091245375 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 73 0.18002466091245375 No Hit ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.17016029593094945 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 66 0.16276202219482122 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 66 0.16276202219482122 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 65 0.16029593094944514 No Hit CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 65 0.16029593094944514 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 60 0.14796547472256474 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 57 0.1405672009864365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 57 0.1405672009864365 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 53 0.13070283600493218 No Hit GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 51 0.12577065351418001 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 51 0.12577065351418001 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 50 0.12330456226880394 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 49 0.12083847102342786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 49 0.12083847102342786 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.11837237977805179 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.1159062885326757 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.11344019728729964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 45 0.11097410604192356 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.11097410604192356 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 44 0.10850801479654747 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 44 0.10850801479654747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 41 0.10110974106041924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.002466091245376079 0.0 12-13 0.0 0.0 0.0 0.002466091245376079 0.0 14-15 0.0 0.0 0.0 0.002466091245376079 0.0 16-17 0.0 0.0 0.0 0.0036991368680641184 0.0 18-19 0.0 0.0 0.0 0.004932182490752158 0.0 20-21 0.0 0.0 0.0 0.009864364981504316 0.0 22-23 0.0 0.0 0.0 0.04932182490752158 0.0 24-25 0.0 0.0 0.0 0.1405672009864365 0.0 26-27 0.0 0.0 0.0 0.2959309494451295 0.0 28-29 0.0 0.0 0.0 0.5548705302096177 0.0 30-31 0.0 0.0 0.0 0.9309494451294698 0.0 32-33 0.0 0.0 0.0 1.3514180024660911 0.0 34-35 0.0 0.0 0.0 1.9876695437731196 0.0 36-37 0.0 0.0 0.0 2.8655980271270036 0.0 38-39 0.0 0.0 0.0 3.5178791615289766 0.0 40-41 0.0 0.0 0.0 3.953144266337854 0.0 42-43 0.0 0.0 0.0 4.3859432799013565 0.0 44-45 0.0 0.0 0.0 4.696670776818742 0.0 46-47 0.0 0.0 0.0 5.066584463625155 0.0 48-49 0.0 0.0 0.0 5.567200986436498 0.0 50-51 0.0 0.0 0.0 5.924784217016029 0.0 52-53 0.0 0.0 0.0 6.168927250308261 0.0 54-55 0.0 0.0 0.0 6.466091245376079 0.0 56-57 0.0 0.0 0.0 6.785450061652281 0.0 58-59 0.0 0.0 0.0 6.997533908754624 0.0 60-61 0.0 0.0 0.0 7.20098643649815 0.0 62-63 0.0 0.0 0.0 7.348951911220715 0.0 64-65 0.0 0.0 0.0 7.528976572133169 0.0 66-67 0.0 0.0 0.0 7.744759556103576 0.0 68-69 0.0 0.0 0.0 8.076448828606658 0.0 70-71 0.0 0.0 0.0 8.384710234278668 0.0 72-73 0.0 0.0 0.0 8.630086313193587 0.0 74-75 0.0 0.0 0.0 8.902589395807645 0.0 76-77 0.0 0.0 0.0 9.13810110974106 0.0 78-79 0.0 0.0 0.0 9.344019728729963 0.0 80-81 0.0 0.0 0.0 9.570900123304561 0.0 82-83 0.0 0.0 0.0 9.769420468557335 0.0 84-85 0.0 0.0 0.0 9.975339087546239 0.0 86-87 0.0 0.0 0.0 10.182490752157829 0.0 88-89 0.0 0.0 0.0 10.3711467324291 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 15 6.571918E-4 94.99877 5 GCGGCCT 15 6.571918E-4 94.99877 9 TGGGCGG 15 6.571918E-4 94.99877 6 GGGCGGC 25 0.004986761 56.999256 7 CTTATAC 1080 0.0 51.521194 1 TATACAC 1205 0.0 48.090664 3 GATATCG 20 5.3587736E-4 47.499386 36-37 CTGATAT 20 5.3587736E-4 47.499386 34-35 GAGTATA 25 2.8038487E-5 47.499382 12-13 TTATACA 1220 0.0 46.720703 2 ATACACA 1380 0.0 41.99221 4 TACACAT 1390 0.0 41.690105 5 ATCGTAT 30 8.223369E-5 39.58282 38-39 CACATCT 1500 0.0 38.63283 7 ATGGGAG 25 0.0016070766 37.999504 18-19 TATCGTA 25 0.0016070766 37.999504 38-39 TGATATC 25 0.0016070766 37.999504 34-35 ATATCGT 25 0.0016070766 37.999504 36-37 ACATCTC 1540 0.0 37.62938 8 ACACATC 1545 0.0 37.507607 6 >>END_MODULE