FastQCFastQC Report
Fri 10 Feb 2017
SRR2031507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792678
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.12757450027277628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.052.1568641
GTATCAA34300.040.7142871
TCAACGC38050.035.8278584
CAACGCA38600.035.1942985
ATCAACG40100.033.8778043
AACGCAG40800.033.6458326
TATCAAC41550.033.152832
ACGCAGA45250.030.02217
CGCAGAG46950.028.5303528
GTACATG47250.028.0476191
TACATGG49300.026.5922932
ACATGGG49600.024.8034273
GCAGAGT57700.022.3916829
CATGGGG33050.020.9833584
GTATATG10900.020.4816511
AGAGTAC47000.020.36436310-11
CAGAGTA52300.020.07170110-11
GAGTACT29650.019.86509112-13
GTACTTT32150.017.87713814-15
TATACGG1350.00940239317.5925925
Produced by FastQC (version 0.11.5)