FastQCFastQC Report
Fri 10 Feb 2017
SRR2031507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT63100.35198736192445046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.15501947365896163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.11954182513535616No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT20480.11424249084330818RNA PCR Primer, Index 23 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.054.4115261
GTATCAA35300.039.49241
TCAACGC39250.034.6104664
CAACGCA40150.033.9529465
ATCAACG41300.033.0075233
AACGCAG42200.032.641246
TATCAAC43500.031.6657562
ACGCAGA46750.029.159597
GTACATG45650.028.349721
CGCAGAG48050.027.9752628
TACATGG47950.026.5477262
ACATGGG48200.025.0304013
GACCGTC2402.2919266E-1023.7493177
GCAGAGT59250.022.5267779
CATGGGG28900.022.5166534
CAGAGTA54200.020.81351510-11
GTATTAC5000.019.9834251
GAGTACT34850.019.6264112-13
AGAGTAC51550.019.30351810-11
TATCTCG1250.00603343318.9994535
Produced by FastQC (version 0.11.5)