FastQCFastQC Report
Fri 10 Feb 2017
SRR2031504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132669
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.21250691949722292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.14390788482778288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.052.425761
GTATCAA25450.044.233651
ATCAACG29350.037.223053
TCAACGC29750.036.4032444
AACGCAG30050.036.0398186
CAACGCA30000.035.9415475
TATCAAC31600.035.023622
ACGCAGA34250.031.481657
CGCAGAG34850.030.5307488
GTACATG31650.028.965151
ACATGGG31250.027.6639123
TACATGG31550.027.2503072
GCAGAGT41550.025.378989
CATGGGG19050.024.4356164
GTATAAT11100.022.6801071
AGAGTAC37400.021.9083510-11
ACACCGT1754.049274E-621.7142146
CTATACT5050.021.6335934
CAGAGTA39550.021.49803510-11
GAGTACT25400.021.41233312-13
Produced by FastQC (version 0.11.5)