##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031504_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1132669 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17215002794285 34.0 31.0 34.0 30.0 34.0 2 32.3297026757155 34.0 31.0 34.0 30.0 34.0 3 32.43219157582666 34.0 31.0 34.0 30.0 34.0 4 35.66586443170953 37.0 35.0 37.0 35.0 37.0 5 35.654558392610724 37.0 35.0 37.0 35.0 37.0 6 35.77599810712574 37.0 37.0 37.0 35.0 37.0 7 35.80057986931752 37.0 37.0 37.0 35.0 37.0 8 35.846328450765405 37.0 37.0 37.0 35.0 37.0 9 37.66572140669516 39.0 39.0 39.0 35.0 39.0 10-11 37.66006882858099 39.0 38.5 39.0 35.0 39.0 12-13 37.62974531835867 39.0 39.0 39.0 35.0 39.0 14-15 39.13450619730919 41.0 39.0 41.0 36.0 41.0 16-17 39.1482772107297 41.0 39.0 41.0 36.0 41.0 18-19 39.10418930861532 41.0 39.5 41.0 36.0 41.0 20-21 39.06825427375517 41.0 39.0 41.0 36.0 41.0 22-23 38.99829473570831 41.0 39.0 41.0 36.0 41.0 24-25 38.95190077595484 41.0 39.0 41.0 35.5 41.0 26-27 38.844758707089184 41.0 39.0 41.0 35.0 41.0 28-29 38.742428723660666 41.0 39.0 41.0 35.0 41.0 30-31 38.57673777599634 40.0 38.5 41.0 34.0 41.0 32-33 38.47319075563999 40.0 38.0 41.0 34.0 41.0 34-35 38.34767085529842 40.0 38.0 41.0 34.0 41.0 36-37 38.20915730897553 40.0 38.0 41.0 34.0 41.0 38-39 38.062648487775334 40.0 38.0 41.0 33.0 41.0 40-41 37.94112092764965 40.0 38.0 41.0 33.0 41.0 42-43 37.795421257225186 40.0 38.0 41.0 33.0 41.0 44-45 37.96163309846036 40.0 38.0 41.0 33.0 41.0 46-47 37.842841553887325 40.0 38.0 41.0 33.0 41.0 48-49 37.71982812277903 40.0 38.0 41.0 32.0 41.0 50-51 36.68077523089269 39.0 36.5 40.0 31.0 40.5 52-53 36.759468123520634 39.5 37.0 40.0 30.5 40.5 54-55 37.55179800983341 40.0 38.0 41.0 32.0 41.0 56-57 37.602075275301075 40.0 37.5 41.0 32.0 41.0 58-59 37.4320741540556 40.0 37.0 41.0 31.5 41.0 60-61 37.28664331768593 40.0 37.0 41.0 31.0 41.0 62-63 37.053776522532175 40.0 36.0 41.0 31.0 41.0 64-65 36.80020200076103 39.0 36.0 41.0 31.0 41.0 66-67 36.53471534932094 39.0 35.0 41.0 30.5 41.0 68-69 36.25414353178201 39.0 35.0 41.0 30.0 41.0 70-71 35.91104991837862 37.5 35.0 40.5 30.0 41.0 72-73 35.55489511940382 37.0 35.0 40.0 29.0 41.0 74-75 35.17798845028865 36.5 35.0 39.0 29.0 41.0 76-77 34.79794935678473 36.0 35.0 39.0 29.0 41.0 78-79 34.38499199677929 36.0 34.5 38.0 28.5 39.5 80-81 34.01643066067845 35.0 34.0 37.0 28.0 39.0 82-83 33.692067144064154 35.0 34.0 37.0 28.0 39.0 84-85 33.37577482918664 35.0 34.0 36.0 27.0 38.0 86-87 33.08890328948704 35.0 34.0 36.0 26.5 37.0 88-89 32.85501015742463 35.0 34.0 36.0 26.0 37.0 90-91 32.65705294309282 35.0 34.0 35.0 26.0 36.5 92-93 32.49243424160103 35.0 34.0 35.0 26.0 36.0 94-95 32.35226045737987 35.0 34.0 35.0 25.0 36.0 96-97 32.218265883501715 35.0 33.0 35.0 25.0 36.0 98-99 32.086861210115224 35.0 33.0 35.0 25.0 35.5 100-101 30.590048372472452 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4611850627898946 1101 2 0.34128757712157665 1101 3 0.4295139299989117 1101 4 0.32516841464403967 1101 5 0.26435138673424063 1101 6 0.36770263178712526 1101 7 0.3140908277614969 1101 8 0.30733399702346986 1101 9 0.6496562090857978 1101 10-11 0.6391390748640973 1101 12-13 0.8075633359528709 1101 14-15 0.8306434129080245 1101 16-17 0.7496583269788815 1101 18-19 0.8755473398635658 1101 20-21 0.8553242231072034 1101 22-23 0.8986626337575885 1101 24-25 0.7973619397376766 1101 26-27 0.7653102018184512 1101 28-29 0.8348530145074449 1101 30-31 0.8460761007245665 1101 32-33 0.8675217293200888 1101 34-35 0.9289417257527219 1101 36-37 0.820537293242019 1101 38-39 0.919230056550056 1101 40-41 0.8659235017950238 1101 42-43 0.892671809381234 1101 44-45 0.9894232183883673 1101 46-47 0.8824226300313285 1101 48-49 0.933002536851042 1101 50-51 0.9061972713869508 1101 52-53 1.0451817128795327 1101 54-55 1.0138086916178892 1101 56-57 0.9452339336678719 1101 58-59 0.9135120657075433 1101 60-61 0.7565545810794063 1101 62-63 0.8619611510667866 1101 64-65 0.7607882029382935 1101 66-67 0.8159471326088337 1101 68-69 0.7977629361470449 1101 70-71 0.8895854293945433 1101 72-73 0.7666151360624625 1101 74-75 0.6941013263074822 1101 76-77 0.6383781273170328 1101 78-79 0.7277423397496534 1101 80-81 0.6718271686718964 1101 82-83 0.5466102826642683 1101 84-85 0.5822244880809606 1101 86-87 0.49984099643813096 1101 88-89 0.5556002933606479 1101 90-91 0.6481065148097613 1101 92-93 0.5639909211470666 1101 94-95 0.48298756359456974 1101 96-97 0.715056349347023 1101 98-99 0.626393894759083 1101 100-101 0.5411345760422073 1102 1 2.729637652905126E-4 1102 2 0.09445089859312361 1102 3 0.12844476894312606 1102 4 0.018905454146363354 1102 5 0.11479364371690792 1102 6 0.005701530944854483 1102 7 0.002925279004429626 1102 8 0.13382013409113824 1102 9 0.07298126476793954 1102 10-11 0.23880050186289026 1102 12-13 0.23893270053647342 1102 14-15 0.3400309733903484 1102 16-17 0.31748653158194173 1102 18-19 0.29810036589977074 1102 20-21 0.2994876789839154 1102 22-23 0.34042519744003386 1102 24-25 0.19339074724388894 1102 26-27 0.3043092993837959 1102 28-29 0.3148474462936264 1102 30-31 0.3692169893346886 1102 32-33 0.29141480681425946 1102 34-35 0.371326713336245 1102 36-37 0.38667023274689427 1102 38-39 0.37276667235610006 1102 40-41 0.4205816006660186 1102 42-43 0.435133439907851 1102 44-45 0.3941811610933641 1102 46-47 0.5345004698203795 1102 48-49 0.44372774869917464 1102 50-51 0.4445499441295837 1102 52-53 0.5003071896978426 1102 54-55 0.5559479097638729 1102 56-57 0.29336970208137814 1102 58-59 0.4986561352398198 1102 60-61 0.4450554195116041 1102 62-63 0.4650955114262274 1102 64-65 0.6225100930588781 1102 66-67 0.6489247189481588 1102 68-69 0.5456308542750961 1102 70-71 0.5477579032175299 1102 72-73 0.4231571472756954 1102 74-75 0.4287183356006423 1102 76-77 0.4891715977863029 1102 78-79 0.4505756965505512 1102 80-81 0.16729659469785219 1102 82-83 0.10841905612117131 1102 84-85 0.4362252817114367 1102 86-87 0.29251303504441495 1102 88-89 0.2319758661826441 1102 90-91 0.19252102032677954 1102 92-93 0.07734903409860294 1102 94-95 0.10563707333574968 1102 96-97 0.13588370911763548 1102 98-99 0.21435247236846777 1102 100-101 -0.10367578467564798 1103 1 0.2661946469039549 1103 2 0.3224969702712741 1103 3 0.3575964610665139 1103 4 0.26794084398363793 1103 5 0.2342680372479009 1103 6 0.33430540436005174 1103 7 0.30603873612732 1103 8 0.3015275315155179 1103 9 0.2742690754897126 1103 10-11 0.31963558222627597 1103 12-13 0.27112360100567656 1103 14-15 0.2564293461566507 1103 16-17 0.27290357261218645 1103 18-19 0.32520740892662303 1103 20-21 0.26990858843783627 1103 22-23 0.2608567138621112 1103 24-25 0.21810311122511195 1103 26-27 0.22360961384920586 1103 28-29 0.31748171803167224 1103 30-31 0.41834534612814167 1103 32-33 0.3508999321012709 1103 34-35 0.3137945180681996 1103 36-37 0.40629249989274996 1103 38-39 0.31129929190286987 1103 40-41 0.23538643245197477 1103 42-43 0.23518148323419297 1103 44-45 0.32058128088971216 1103 46-47 0.3810650600170433 1103 48-49 0.45941233490263045 1103 50-51 0.3722709434307703 1103 52-53 0.44195326636751275 1103 54-55 0.3863162835284726 1103 56-57 0.4607645059148595 1103 58-59 0.5373840477884855 1103 60-61 0.49813455997281864 1103 62-63 0.5790711278782581 1103 64-65 0.7246420936578559 1103 66-67 0.6270431675854695 1103 68-69 0.6212429387315197 1103 70-71 0.5589413912878172 1103 72-73 0.6428199704762605 1103 74-75 0.5819004260468859 1103 76-77 0.5119360232629901 1103 78-79 0.5411198964107911 1103 80-81 0.5299794089136896 1103 82-83 0.3953550149910896 1103 84-85 0.488693585594838 1103 86-87 0.3975831658220912 1103 88-89 0.6171030250126321 1103 90-91 0.3876318319470258 1103 92-93 0.4498401741604994 1103 94-95 0.41234941235940425 1103 96-97 0.46200056047033655 1103 98-99 0.42494945749376 1103 100-101 0.3503511195871063 1104 1 0.3043553407347517 1104 2 0.2965492257668245 1104 3 0.37224218189780345 1104 4 0.27291002524147956 1104 5 0.2519410858487845 1104 6 0.1977701946289656 1104 7 0.24253576334599813 1104 8 0.37282519147296966 1104 9 0.4597542661295364 1104 10-11 0.5577608208669744 1104 12-13 0.5783182772725155 1104 14-15 0.611643986422834 1104 16-17 0.6419102818250835 1104 18-19 0.7462050634294286 1104 20-21 0.772144992736095 1104 22-23 0.6023611048510418 1104 24-25 0.6279596540713541 1104 26-27 0.6840907135055616 1104 28-29 0.7271091658034479 1104 30-31 0.7112406714976913 1104 32-33 0.7461925737856703 1104 34-35 0.7041316151063342 1104 36-37 0.6561861343224678 1104 38-39 0.6754813679406126 1104 40-41 0.7163606319828801 1104 42-43 0.5278695321078075 1104 44-45 0.5797287199340531 1104 46-47 0.7448462196744927 1104 48-49 0.8619951091348881 1104 50-51 0.7331720507236312 1104 52-53 0.6916014417888832 1104 54-55 0.7265470149963207 1104 56-57 0.6824221724178869 1104 58-59 0.6898713657397195 1104 60-61 0.7693831281286023 1104 62-63 0.6997033211908672 1104 64-65 0.7525589648736997 1104 66-67 0.795542594847781 1104 68-69 0.680717656453659 1104 70-71 0.6855764210156252 1104 72-73 0.6193568166007353 1104 74-75 0.7287912786078365 1104 76-77 0.6923920315540428 1104 78-79 0.622372282817814 1104 80-81 0.7099281352541666 1104 82-83 0.5966095832559617 1104 84-85 0.41697319067856853 1104 86-87 0.6537632781877747 1104 88-89 0.7561528259181074 1104 90-91 0.7830335665240185 1104 92-93 0.4825462233613962 1104 94-95 0.5571129535146966 1104 96-97 0.6233107661104356 1104 98-99 0.644395265599357 1104 100-101 0.7118440558223078 1105 1 0.13378869017098083 1105 2 0.06742936884275252 1105 3 0.17988831566146501 1105 4 0.10333730011787878 1105 5 0.07372110661124509 1105 6 0.01328961574705545 1105 7 0.12623106918106686 1105 8 0.09890365710699456 1105 9 0.41366675546565546 1105 10-11 0.3307393080857324 1105 12-13 0.3491603901092688 1105 14-15 0.38200763773693325 1105 16-17 0.2579610984473817 1105 18-19 0.3298864933664234 1105 20-21 0.3995524610527994 1105 22-23 0.39284223876887125 1105 24-25 0.39529270646057313 1105 26-27 0.32357638021677104 1105 28-29 0.3066797324540076 1105 30-31 0.4782771908057484 1105 32-33 0.34306998279575396 1105 34-35 0.46022981535095653 1105 36-37 0.42655235524732404 1105 38-39 0.4567781990966395 1105 40-41 0.30968786677335913 1105 42-43 0.29997229598114217 1105 44-45 0.28850291281931817 1105 46-47 0.40478701584268606 1105 48-49 0.48177751449612316 1105 50-51 0.4740175554465793 1105 52-53 0.45189170501980414 1105 54-55 0.4599904989773691 1105 56-57 0.40034417959223845 1105 58-59 0.3811701497999067 1105 60-61 0.39781330152593597 1105 62-63 0.48578168404046806 1105 64-65 0.4029911734027465 1105 66-67 0.3375228396802967 1105 68-69 0.33735650280122087 1105 70-71 0.3099877231915684 1105 72-73 0.37145259113280815 1105 74-75 0.30214287330827716 1105 76-77 0.27520441913357985 1105 78-79 0.19472943815592458 1105 80-81 0.2454894924197788 1105 82-83 0.34273615200333296 1105 84-85 0.2708276068116078 1105 86-87 0.21978428732531086 1105 88-89 0.19393021539914912 1105 90-91 0.26977745855924695 1105 92-93 0.3167611044911993 1105 94-95 0.3888358261339562 1105 96-97 0.4073370378108052 1105 98-99 0.485129763137671 1105 100-101 0.49143158190385705 1106 1 0.3626160308372519 1106 2 0.22962343488105574 1106 3 0.24367693075041785 1106 4 0.14154583941894572 1106 5 0.2322797490823021 1106 6 0.3073207729902876 1106 7 0.2507792549383794 1106 8 0.2785154413918889 1106 9 0.28158064962683227 1106 10-11 0.25124946493643563 1106 12-13 0.4227651240485315 1106 14-15 0.31972974310814095 1106 16-17 0.31085960027269977 1106 18-19 0.3489917134544953 1106 20-21 0.34905476443815786 1106 22-23 0.324935377845442 1106 24-25 0.3675722453463024 1106 26-27 0.4353668429562916 1106 28-29 0.3945538603087684 1106 30-31 0.348403703545884 1106 32-33 0.40106663155300737 1106 34-35 0.3915526248062875 1106 36-37 0.33322366638540757 1106 38-39 0.57033409955541 1106 40-41 0.5134103504186953 1106 42-43 0.5590278271949316 1106 44-45 0.5951587318255918 1106 46-47 0.5700543216469143 1106 48-49 0.4484323640561172 1106 50-51 0.40968576530698897 1106 52-53 0.5186551895899285 1106 54-55 0.4816633260633125 1106 56-57 0.5064966468805565 1106 58-59 0.6245116220466613 1106 60-61 0.5105484991741704 1106 62-63 0.5664448423253745 1106 64-65 0.5582266110364671 1106 66-67 0.6750783333518058 1106 68-69 0.5234854372561912 1106 70-71 0.6815893149843504 1106 72-73 0.6922780081135116 1106 74-75 0.6602168876807966 1106 76-77 0.6803402984356168 1106 78-79 0.5527782659337959 1106 80-81 0.6031244046516306 1106 82-83 0.6282076688160956 1106 84-85 0.6979727680866077 1106 86-87 0.6776765780413427 1106 88-89 0.6298902095824701 1106 90-91 0.6676748056029638 1106 92-93 0.5760894112772235 1106 94-95 0.6201376309197926 1106 96-97 0.639311277151883 1106 98-99 0.5658147814858623 1106 100-101 0.4368108872670824 1107 1 0.1563746000365498 1107 2 0.10740507774205099 1107 3 0.19715962152104538 1107 4 0.1373281779949238 1107 5 0.2197037714301544 1107 6 0.17724065955157187 1107 7 0.020990615981702376 1107 8 0.08652112040882542 1107 9 0.03525417519347229 1107 10-11 0.0699811924604603 1107 12-13 0.11621947284249501 1107 14-15 0.18640827056454157 1107 16-17 0.131589516813456 1107 18-19 0.0682915196987608 1107 20-21 0.21926231940717145 1107 22-23 0.23856227457603296 1107 24-25 0.3115490442253659 1107 26-27 0.3015111923078848 1107 28-29 0.2202746024401563 1107 30-31 0.3162215749235884 1107 32-33 0.1190993691029405 1107 34-35 0.12901315891357967 1107 36-37 0.23934286024116602 1107 38-39 0.21161026742276334 1107 40-41 0.19909809477088913 1107 42-43 0.2503264331980901 1107 44-45 0.11891881460329046 1107 46-47 0.29815102779939195 1107 48-49 0.10816622209662796 1107 50-51 0.10106491846430288 1107 52-53 0.066594023320242 1107 54-55 0.04322038221530278 1107 56-57 0.12934566168264183 1107 58-59 0.05977870667339147 1107 60-61 0.07619088928263551 1107 62-63 0.01609047910374528 1107 64-65 0.17934873025864562 1107 66-67 0.15644582930011097 1107 68-69 0.12986477186588985 1107 70-71 0.06205556793959488 1107 72-73 0.1384663131978101 1107 74-75 0.24362390838317793 1107 76-77 0.11645180048071069 1107 78-79 0.018392154741547984 1107 80-81 0.1478302880459026 1107 82-83 0.16975185512608704 1107 84-85 0.2869264782566958 1107 86-87 0.2533789536513069 1107 88-89 0.27422230107340795 1107 90-91 0.36489229973673787 1107 92-93 0.32420164267237794 1107 94-95 0.2763057284048571 1107 96-97 0.33879382807140246 1107 98-99 0.346484880700487 1107 100-101 0.25417862397171476 1108 1 0.21809641390005652 1108 2 0.1864881852058815 1108 3 0.19611098637349755 1108 4 0.027609407425785548 1108 5 0.1735312897187029 1108 6 0.1913484312144007 1108 7 0.28701108313740065 1108 8 0.20670872348915736 1108 9 0.2854700338604772 1108 10-11 0.29282116673261527 1108 12-13 0.31476940314204427 1108 14-15 0.2727016831836977 1108 16-17 0.24903415062956213 1108 18-19 0.22872143508215714 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-0.23823859131553604 2316 94-95 -0.1872137337790818 2316 96-97 -0.190981642511872 2316 98-99 -0.1015619053189809 2316 100-101 0.03381129208837663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 21.0 7 325.0 8 645.0 9 505.0 10 605.0 11 931.0 12 1320.0 13 1631.0 14 1826.0 15 2028.0 16 2358.0 17 2738.0 18 3416.0 19 4243.0 20 5303.0 21 6654.0 22 7789.0 23 7591.0 24 7147.0 25 6971.0 26 7217.0 27 7570.0 28 8969.0 29 10447.0 30 12439.0 31 15214.0 32 19374.0 33 25656.0 34 36589.0 35 57813.0 36 105278.0 37 226958.0 38 378007.0 39 156893.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04255612186791 20.379740241853533 14.353354775313882 23.224348860964678 2 13.978576265440301 21.4763536390596 42.111949739950504 22.43312035554959 3 16.873243639580497 28.093291155668602 31.562089189339517 23.471376015411387 4 11.276727799560154 18.793840036233004 42.107535387655176 27.821896776551668 5 12.10653774403643 39.03982540353802 36.59762913966922 12.25600771275633 6 27.225341207360664 40.55227078696424 20.032595577348722 12.189792428326369 7 24.656188171478163 34.11888203879509 24.665281737206545 16.5596480525202 8 23.933117265502986 36.62058377160494 22.12747060262089 17.318828360271183 9 25.772224718783686 16.58048379535416 21.52959072774129 36.11770075812086 10-11 22.790373886810713 27.385449765112313 30.966460634130538 18.85771571394644 12-13 24.284455564688358 25.116825833495927 30.042448411671902 20.55627019014381 14-15 21.507518966264637 26.426343441905797 27.9084622250631 24.15767536676646 16-17 20.935772056973395 29.55227873279837 28.133550048602018 21.378399161626213 18-19 20.823206073442464 28.78700661887983 30.025585585903734 20.36420172177397 20-21 21.95720903458998 28.025265986797553 29.789020446396965 20.228504532215503 22-23 21.437683912952505 27.944704057407773 29.469686201352737 21.147925828286994 24-25 21.155474370712007 28.103355878901958 29.465669140763985 21.275500609622053 26-27 21.29324630584928 28.312728608269495 29.316375746135897 21.077649339745328 28-29 21.27934109611899 28.243864712462337 29.41503651993654 21.061757671482138 30-31 21.54521753486676 28.089847960878245 29.44364152281028 20.92129298144471 32-33 21.299647116677512 28.220777649957757 29.33526917395991 21.144306059404823 34-35 21.538860867561485 28.2527817040989 29.21630237960075 20.992055048738862 36-37 21.669084260273745 28.0498980726055 29.126337879821907 21.15467978729885 38-39 21.483725607392802 28.283064160844873 29.158739225669635 21.07447100609269 40-41 21.64758636459548 28.132976182803628 29.20403048021973 21.01540697238116 42-43 21.404178979030945 28.171292760727095 29.141258390580127 21.283269869661833 44-45 21.66290416706028 28.356121691332596 28.854104773768857 21.126869367838268 46-47 21.547011810615206 28.18836949060514 28.73747149980025 21.527147198979403 48-49 21.53073571211952 28.13264651624554 28.889534367855617 21.447083403779317 50-51 21.52813399148383 28.38812574547374 28.663184036995805 21.42055622604662 52-53 21.54424637736179 28.396601301880782 28.576397870869602 21.482754449887832 54-55 21.563139805185806 28.31400877043514 28.637580793682886 21.48527063069617 56-57 21.54093561314029 28.387816740813072 28.56315481398361 21.50809283206303 58-59 21.578899042880135 28.2401125130113 28.643319451666816 21.53766899244175 60-61 21.61032923122289 28.328090554257244 28.55167749801575 21.509902716504115 62-63 21.471365420965878 28.202943666684614 28.722733649459816 21.60295726288969 64-65 21.58212152005573 28.114568333732098 28.63237185797439 21.67093828823778 66-67 21.507077531035105 28.279532679008607 28.42864949954488 21.78474029041141 68-69 21.620173236841477 28.094174026127668 28.540111895001978 21.74554084202887 70-71 21.61421386124278 27.8964551868198 28.564699837286973 21.924631114650442 72-73 21.5664505694073 28.07660490399225 28.421498248826442 21.935446277774002 74-75 21.48540306126503 28.103311735379005 28.37404396165164 22.03724124170433 76-77 21.49462905756227 28.028576751019052 28.453281585352823 22.02351260606585 78-79 21.55744529072483 28.048662053962808 28.302531454467278 22.091361200845082 80-81 21.703825212837994 28.035242422985 28.161890190338042 22.09904217383896 82-83 21.74602642078136 28.043717979392035 28.214288552083616 21.995967047742987 84-85 21.74470211509276 27.891952547478567 28.31656909476643 22.04677624266224 86-87 21.65822495362723 27.8744275688661 28.387905027858977 22.07944244964769 88-89 21.814713742496707 27.700546231953023 28.34693983855831 22.137800186991964 90-91 21.923968961806143 27.747691514467153 28.27569219251167 22.052647331215034 92-93 21.998748089689045 27.6809023642388 28.201575217473067 22.118774328599088 94-95 21.840846708085063 27.57654707597718 28.371262919705583 22.211343296232176 96-97 21.82760365119907 27.569572399350562 28.412272252529203 22.190551696921165 98-99 21.905825973872332 27.431712177167384 28.262051843919096 22.40041000504119 100-101 22.106369998649207 27.3812119869088 28.24876464351015 22.263653370931845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 11.0 2 26.5 3 40.5 4 38.0 5 45.0 6 72.5 7 117.5 8 247.5 9 348.0 10 364.5 11 396.5 12 424.0 13 472.5 14 550.0 15 644.5 16 719.0 17 820.5 18 929.5 19 1004.0 20 1179.5 21 1479.5 22 1871.5 23 2379.0 24 3208.5 25 4422.5 26 6103.0 27 8505.0 28 11455.0 29 15192.0 30 19464.5 31 23491.5 32 27450.5 33 31373.5 34 35226.0 35 37812.5 36 40022.5 37 41444.5 38 41387.5 39 41259.0 40 40561.5 41 40080.5 42 39990.0 43 40268.5 44 40971.0 45 41630.5 46 41505.0 47 41160.0 48 40525.5 49 39514.0 50 38176.5 51 35976.0 52 33605.0 53 31548.0 54 29316.5 55 26498.5 56 23559.5 57 20492.0 58 17785.5 59 15273.0 60 12406.0 61 9774.5 62 7572.0 63 5657.5 64 4122.5 65 3035.0 66 2343.5 67 1766.0 68 1300.5 69 1011.0 70 770.5 71 565.0 72 444.5 73 344.0 74 269.0 75 205.0 76 152.5 77 117.5 78 79.0 79 49.0 80 40.5 81 36.0 82 29.5 83 27.0 84 27.0 85 17.5 86 13.5 87 9.5 88 8.0 89 8.0 90 5.5 91 6.5 92 3.5 93 1.5 94 3.0 95 2.0 96 2.0 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.3243056885992288E-4 48-49 3.972917065797687E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1132669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.51518844996896 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39967591792879 49.39823976829691 2 14.751710843513663 19.329222317444962 3 4.918620996184408 9.667331444369866 4 2.083932673372329 5.46116967252143 5 1.025849766313957 3.360437038070775 6 0.5676761179143103 2.23148447062217 7 0.35335374044012297 1.6205025826105253 8 0.21845052674955645 1.1449461941593742 9 0.1538485742061717 0.9071476498688736 >10 0.506547470056151 5.241288461370793 >50 0.014917512754924369 0.6816357753151415 >100 0.005009673235892852 0.5100546713072309 >500 1.3539577658163408E-4 0.08843868378621424 >1k 2.7079155316326816E-4 0.35810127025571403 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2407 0.21250691949722292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.14390788482778288 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.7657409181323053E-4 0.0 24-25 0.0 0.0 0.0 6.180093213463069E-4 0.0 26-27 0.0 0.0 0.0 0.0013243056885992289 0.0 28-29 0.0 0.0 0.0 0.003708055928077841 0.0 30-31 0.0 0.0 0.0 0.01359620506961875 0.0 32-33 0.0 0.0 0.0 0.03447609142653326 0.0 34-35 0.0 0.0 0.0 0.06559727510861514 0.0 36-37 0.0 0.0 0.0 0.11927579901983723 0.0 38-39 0.0 0.0 0.0 0.2122420583595031 0.0 40-41 0.0 0.0 0.0 0.313110008307811 0.0 42-43 0.0 0.0 0.0 0.40815101322628233 0.0 44-45 0.0 0.0 0.0 0.5138747506994541 0.0 46-47 0.0 0.0 0.0 0.6524854127728401 0.0 48-49 0.0 0.0 0.0 0.8058841550355841 0.0 50-51 0.0 0.0 0.0 0.9643594024379585 0.0 52-53 0.0 0.0 0.0 1.118994163343395 0.0 54-55 0.0 0.0 0.0 1.275394665166964 0.0 56-57 0.0 0.0 0.0 1.4614596144151557 0.0 58-59 0.0 0.0 0.0 1.6732602375451258 0.0 60-61 0.0 0.0 0.0 1.8909319492278858 0.0 62-63 0.0 0.0 0.0 2.1048514614596145 0.0 64-65 0.0 0.0 0.0 2.3281294005574447 0.0 66-67 0.0 0.0 0.0 2.580188916620831 0.0 68-69 0.0 0.0 0.0 2.8732577655078404 0.0 70-71 0.0 0.0 0.0 3.17727420808727 0.0 72-73 0.0 0.0 0.0 3.476832154848416 0.0 74-75 0.0 0.0 0.0 3.760454289823417 0.0 76-77 0.0 0.0 0.0 4.063941010127407 0.0 78-79 0.0 0.0 0.0 4.398239909452806 0.0 80-81 0.0 0.0 0.0 4.766661752021111 0.0 82-83 0.0 0.0 0.0 5.143912299180078 0.0 84-85 0.0 0.0 0.0 5.514806179033769 0.0 86-87 0.0 0.0 8.828704590661526E-5 5.905829505354168 0.0 88-89 0.0 0.0 8.828704590661526E-5 6.351016934338276 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1350 0.0 52.42576 1 GTATCAA 2545 0.0 44.23365 1 ATCAACG 2935 0.0 37.22305 3 TCAACGC 2975 0.0 36.403244 4 AACGCAG 3005 0.0 36.039818 6 CAACGCA 3000 0.0 35.941547 5 TATCAAC 3160 0.0 35.02362 2 ACGCAGA 3425 0.0 31.48165 7 CGCAGAG 3485 0.0 30.530748 8 GTACATG 3165 0.0 28.96515 1 ACATGGG 3125 0.0 27.663912 3 TACATGG 3155 0.0 27.250307 2 GCAGAGT 4155 0.0 25.37898 9 CATGGGG 1905 0.0 24.435616 4 GTATAAT 1110 0.0 22.680107 1 AGAGTAC 3740 0.0 21.90835 10-11 ACACCGT 175 4.049274E-6 21.714214 6 CTATACT 505 0.0 21.633593 4 CAGAGTA 3955 0.0 21.498035 10-11 GAGTACT 2540 0.0 21.412333 12-13 >>END_MODULE