FastQCFastQC Report
Fri 10 Feb 2017
SRR2031504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132669
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.25700359063415706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.19431978804046018No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17610.15547348784154946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.11415515035725352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.054.2984771
GTATCAA26400.040.886941
TCAACGC31550.034.1750874
ATCAACG32250.033.43333
CAACGCA32400.033.4251175
AACGCAG33650.032.3246276
TATCAAC34250.031.4809912
ACGCAGA37500.028.752647
CGCAGAG38400.027.9550538
GTACATG31050.025.1156791
TACATGG30550.023.9437752
GCAGAGT44950.023.8815149
ACATGGG30400.022.8119493
CATGGGG17950.021.698644
GAGTACT29500.021.65625612-13
AGAGTAC41350.021.25099810-11
GTATAAG5650.021.0404111
CAGAGTA43750.020.68235810-11
TCGTGTA1707.955203E-519.5583529
ATAAGCG1250.00603193318.999545
Produced by FastQC (version 0.11.5)