FastQCFastQC Report
Fri 10 Feb 2017
SRR2031502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936864
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.35106482904669195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.28061703726474707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.15872100966629094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.057.4018141
GTATCAA29800.046.0654371
TCAACGC37650.036.20854
CAACGCA38050.035.7030225
TATCAAC38950.035.1219522
ATCAACG38850.034.9678233
AACGCAG39400.034.4796946
ACGCAGA43200.031.1168987
CGCAGAG45550.029.0944028
GTACATG29800.026.937921
TACATGG29850.026.2562832
GCAGAGT49050.025.8562729
GAGTACT28900.024.160912-13
ACATGGG32400.023.3101843
CAGAGTA47400.022.2969410-11
AGAGTAC44300.022.1952610-11
GTATAGA2901.05501385E-1021.2931061
GTACTTT33900.020.73746514-15
CGTATCA1950.020.7051394-95
GGCGACG1404.8508155E-420.3571438
Produced by FastQC (version 0.11.5)