##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031502_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 936864 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07538127198825 34.0 31.0 34.0 30.0 34.0 2 32.233800210062505 34.0 31.0 34.0 30.0 34.0 3 32.32722679065478 34.0 31.0 34.0 30.0 34.0 4 35.55413592581207 37.0 35.0 37.0 33.0 37.0 5 35.524684479284076 37.0 35.0 37.0 33.0 37.0 6 35.6555028264508 37.0 37.0 37.0 35.0 37.0 7 35.68349621716706 37.0 37.0 37.0 35.0 37.0 8 35.73389414045155 37.0 37.0 37.0 35.0 37.0 9 37.550401125456844 39.0 39.0 39.0 35.0 39.0 10-11 37.55209454093657 39.0 39.0 39.0 35.0 39.0 12-13 37.519346457970414 39.0 39.0 39.0 35.0 39.0 14-15 39.01569971735492 41.0 39.0 41.0 36.0 41.0 16-17 39.02332195494757 41.0 39.0 41.0 36.0 41.0 18-19 38.99290878932268 41.0 39.5 41.0 36.0 41.0 20-21 38.9521904993681 41.0 39.0 41.0 36.0 41.0 22-23 38.878388965741024 41.0 39.0 41.0 35.0 41.0 24-25 38.820020835468114 41.0 39.0 41.0 35.0 41.0 26-27 38.70163278768317 41.0 39.0 41.0 35.0 41.0 28-29 38.590822147077915 41.0 39.0 41.0 35.0 41.0 30-31 38.40600663490112 40.0 38.5 41.0 34.0 41.0 32-33 38.27090218003894 40.0 38.0 41.0 34.0 41.0 34-35 38.1195952667623 40.0 38.0 41.0 33.5 41.0 36-37 37.96529432233494 40.0 38.0 41.0 33.0 41.0 38-39 37.80663735611572 40.0 38.0 41.0 33.0 41.0 40-41 37.64899761331421 40.0 38.0 41.0 33.0 41.0 42-43 37.47642560713188 40.0 37.5 41.0 32.0 41.0 44-45 37.615787883833725 40.0 38.0 41.0 32.5 41.0 46-47 37.44580430030399 40.0 38.0 41.0 31.5 41.0 48-49 37.26173275950404 40.0 37.5 41.0 31.0 41.0 50-51 36.187190456672475 39.0 36.0 40.0 29.5 40.5 52-53 36.23871287614851 39.0 36.5 40.0 29.5 40.5 54-55 36.9704802404618 40.0 37.0 41.0 30.5 41.0 56-57 36.9744242494108 40.0 36.5 41.0 31.0 41.0 58-59 36.75873712726714 40.0 36.0 41.0 30.0 41.0 60-61 36.56992903986064 39.5 35.0 41.0 30.0 41.0 62-63 36.293871896027596 39.0 35.0 41.0 29.0 41.0 64-65 35.98216496738054 39.0 35.0 41.0 29.0 41.0 66-67 35.669560896779046 38.0 35.0 41.0 29.0 41.0 68-69 35.344523324623424 37.0 35.0 40.0 28.0 41.0 70-71 34.966690469481165 37.0 35.0 39.5 28.0 41.0 72-73 34.6004073163234 36.0 35.0 39.0 27.5 41.0 74-75 34.22138432045633 36.0 34.5 39.0 27.0 41.0 76-77 33.859790748710594 35.0 34.0 37.5 26.0 39.5 78-79 33.473566067220005 35.0 34.0 37.0 26.0 39.0 80-81 33.156229719575094 35.0 34.0 36.5 26.0 39.0 82-83 32.88078685913857 35.0 34.0 36.0 25.0 38.0 84-85 32.61308578406258 35.0 34.0 36.0 25.0 37.0 86-87 32.373718597363116 35.0 34.0 35.0 24.0 37.0 88-89 32.17970911466339 35.0 33.5 35.0 24.0 36.0 90-91 32.00588132322301 35.0 33.0 35.0 23.5 36.0 92-93 31.8466079388257 35.0 33.0 35.0 23.0 36.0 94-95 31.718506634901118 35.0 33.0 35.0 22.0 36.0 96-97 31.59770414916146 35.0 33.0 35.0 20.0 35.0 98-99 31.46167533387984 35.0 33.0 35.0 20.0 35.0 100-101 29.873225462820642 33.5 29.0 34.5 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09062915779406211 1101 2 -0.09990955647989352 1101 3 -0.03158800899927883 1101 4 -0.10317723943069979 1101 5 -0.10842341484084983 1101 6 -0.15799532012385953 1101 7 -0.1997269937672641 1101 8 -0.10003127575288318 1101 9 0.22319854181737497 1101 10-11 0.23320268691924895 1101 12-13 0.32221123983636346 1101 14-15 0.29562576550394226 1101 16-17 0.37996747150412347 1101 18-19 0.4353867477749276 1101 20-21 0.4125875308190743 1101 22-23 0.3678855516374355 1101 24-25 0.3061928294460188 1101 26-27 0.162267664705098 1101 28-29 0.21157461669168498 1101 30-31 0.35297518403222483 1101 32-33 0.2747637636935565 1101 34-35 0.30300636983534446 1101 36-37 0.35291443012135204 1101 38-39 0.2841652797918286 1101 40-41 0.28640636149166454 1101 42-43 0.25065603162016004 1101 44-45 0.4033108919106638 1101 46-47 0.368637840340611 1101 48-49 0.5938226787672534 1101 50-51 0.6274101750008114 1101 52-53 0.7584119500717179 1101 54-55 0.5436544667349992 1101 56-57 0.6383895982325285 1101 58-59 0.6369621978348121 1101 60-61 0.5495015739869515 1101 62-63 0.3840561701314371 1101 64-65 0.391096680015238 1101 66-67 0.49512990843004445 1101 68-69 0.4164720817845904 1101 70-71 0.3707173382614002 1101 72-73 0.5178806995033014 1101 74-75 0.5437040841165697 1101 76-77 0.5891469624838166 1101 78-79 0.7655041425264812 1101 80-81 0.6645498693834995 1101 82-83 0.5171025963914389 1101 84-85 0.6890513970522036 1101 86-87 0.635386428862283 1101 88-89 0.5172307612850275 1101 90-91 0.590701466671419 1101 92-93 0.6185998952109237 1101 94-95 0.7695532843502058 1101 96-97 0.6639467577555997 1101 98-99 0.6992432041893615 1101 100-101 0.5444251074443649 1102 1 0.07701638780566356 1102 2 0.030672225012942533 1102 3 0.2095302516030202 1102 4 0.24199618584375315 1102 5 0.10799903322511284 1102 6 0.06692633615266175 1102 7 0.021232374404803522 1102 8 0.1841910667411497 1102 9 0.4452537198530706 1102 10-11 0.2907059776897185 1102 12-13 0.2667692743276433 1102 14-15 0.4456214223550887 1102 16-17 0.24603478362953268 1102 18-19 0.17254272027278006 1102 20-21 0.2869970296196982 1102 22-23 0.19765082114652444 1102 24-25 0.21492656769446228 1102 26-27 0.3127343861622549 1102 28-29 0.17806889375784607 1102 30-31 0.346938207131096 1102 32-33 0.34308817686732596 1102 34-35 0.22650347328915643 1102 36-37 0.14008677294947347 1102 38-39 0.0995413630733708 1102 40-41 0.21914100846047546 1102 42-43 0.08951518664385105 1102 44-45 0.2650150098839532 1102 46-47 0.28604117186986144 1102 48-49 0.48134180532661475 1102 50-51 0.38636207295686376 1102 52-53 0.43888648919680406 1102 54-55 0.6349574782075891 1102 56-57 0.4866800680276029 1102 58-59 0.5430165473313409 1102 60-61 0.6930994384940661 1102 62-63 0.6282781117194176 1102 64-65 0.6765518086058506 1102 66-67 0.6654381383630295 1102 68-69 0.5545908969735862 1102 70-71 0.46241457401419694 1102 72-73 0.41753391577206855 1102 74-75 0.325277105812269 1102 76-77 0.33399364932942177 1102 78-79 0.1472936534879139 1102 80-81 0.19935519608851848 1102 82-83 0.16062362057275692 1102 84-85 0.14900422377392886 1102 86-87 0.34774636231188794 1102 88-89 0.2844914370789908 1102 90-91 0.03824959210155754 1102 92-93 0.22099464066898733 1102 94-95 0.1930169789667069 1102 96-97 0.16428084612865845 1102 98-99 0.20625237825608522 1102 100-101 0.07522137367270787 1103 1 0.25827115112177523 1103 2 0.3407658682793766 1103 3 0.3768196777469228 1103 4 0.31135536104910955 1103 5 0.21867203686640124 1103 6 0.16686952919751263 1103 7 0.18561264933634902 1103 8 0.3720615468834154 1103 9 0.4416106651208054 1103 10-11 0.48012277137815573 1103 12-13 0.4730305651510278 1103 14-15 0.49764830157150897 1103 16-17 0.5796556801209007 1103 18-19 0.6208933779632346 1103 20-21 0.47279017467855056 1103 22-23 0.41358517206531786 1103 24-25 0.47488983723464173 1103 26-27 0.5972710742814868 1103 28-29 0.6183483769204088 1103 30-31 0.5565197057056324 1103 32-33 0.5481789268602739 1103 34-35 0.5889671309513886 1103 36-37 0.4831099261941745 1103 38-39 0.6305320269737891 1103 40-41 0.5375298785313305 1103 42-43 0.6297978453504882 1103 44-45 0.7536068677889389 1103 46-47 0.7992796622867857 1103 48-49 0.7782119538009411 1103 50-51 0.8896928658549328 1103 52-53 0.8104295566312487 1103 54-55 0.9105320752663246 1103 56-57 0.7696819098431646 1103 58-59 0.9012629060408059 1103 60-61 0.824559906498699 1103 62-63 0.9063252226799889 1103 64-65 0.9294883047810885 1103 66-67 0.8777223777849414 1103 68-69 1.027230938467362 1103 70-71 1.0946309979281637 1103 72-73 1.0355406620390326 1103 74-75 0.8809214589892278 1103 76-77 1.0291096625396818 1103 78-79 0.9620641675026533 1103 80-81 0.9386941889470748 1103 82-83 0.8173640657134698 1103 84-85 0.8309329081107322 1103 86-87 0.8535787782658204 1103 88-89 0.876298180523257 1103 90-91 0.7495599980066601 1103 92-93 0.5939102488209649 1103 94-95 0.8547095086756862 1103 96-97 0.8127234181782264 1103 98-99 0.8714874038015772 1103 100-101 0.7579518828888432 1104 1 0.26783202675407836 1104 2 0.22707103137025797 1104 3 0.1877498190342024 1104 4 0.18013043518132577 1104 5 0.12907017047881908 1104 6 0.0634659882939701 1104 7 0.18186243130041646 1104 8 0.21737595469782178 1104 9 0.3440068800170195 1104 10-11 0.3456763646103198 1104 12-13 0.29676486656389756 1104 14-15 0.2980854636158128 1104 16-17 0.29938139091804317 1104 18-19 0.41375379135936186 1104 20-21 0.3782225399323451 1104 22-23 0.3288400560701987 1104 24-25 0.3368240339902684 1104 26-27 0.324121109167244 1104 28-29 0.47000217946706613 1104 30-31 0.3232376108163919 1104 32-33 0.36559563713412047 1104 34-35 0.34467967237821995 1104 36-37 0.47613822725818267 1104 38-39 0.5115988767906359 1104 40-41 0.45311726733300617 1104 42-43 0.5408119304717189 1104 44-45 0.3897648566255043 1104 46-47 0.4219902849973991 1104 48-49 0.32303469058795997 1104 50-51 0.28268083027146673 1104 52-53 0.2849466070411637 1104 54-55 0.2602671616085601 1104 56-57 0.2806981753237139 1104 58-59 0.29422361596579805 1104 60-61 0.16500121122572153 1104 62-63 0.05919021483069287 1104 64-65 0.17046706808127965 1104 66-67 0.18617236034206286 1104 68-69 0.26261708010350304 1104 70-71 0.4180709508324014 1104 72-73 0.23606830899525022 1104 74-75 0.22050152178357507 1104 76-77 0.3215176513762472 1104 78-79 0.31866193481470617 1104 80-81 0.3780038871853364 1104 82-83 0.19039629142426406 1104 84-85 0.1712760966682083 1104 86-87 0.2015859298443985 1104 88-89 0.2250575560683501 1104 90-91 0.24002637686589168 1104 92-93 0.27837409846338446 1104 94-95 0.3328241519688966 1104 96-97 0.1268477858740198 1104 98-99 0.1675856507926774 1104 100-101 0.09989218465057093 1105 1 0.3154068558709682 1105 2 0.2584775791319416 1105 3 0.3357291761257102 1105 4 0.33735431203137267 1105 5 0.2440511137307908 1105 6 0.23337486965144905 1105 7 0.37262390243571986 1105 8 0.17504886330531377 1105 9 0.3026754181248066 1105 10-11 0.3739633683646062 1105 12-13 0.40492189644989907 1105 14-15 0.3919299628341406 1105 16-17 0.33388751304098463 1105 18-19 0.4075553558385039 1105 20-21 0.5290497112034416 1105 22-23 0.49832315604395205 1105 24-25 0.4528783457221479 1105 26-27 0.562087257764226 1105 28-29 0.5172910147231917 1105 30-31 0.5312300337620854 1105 32-33 0.4344916295114274 1105 34-35 0.4830878633565021 1105 36-37 0.5165175649506111 1105 38-39 0.6156371671607062 1105 40-41 0.4987400635399126 1105 42-43 0.5900007349175311 1105 44-45 0.7164561430130476 1105 46-47 0.6210420120865123 1105 48-49 0.525011257634624 1105 50-51 0.4731612999575461 1105 52-53 0.5733575081857012 1105 54-55 0.6722202785850371 1105 56-57 0.6592549119412041 1105 58-59 0.6982958547342974 1105 60-61 0.7197415772978601 1105 62-63 0.7391737826647287 1105 64-65 0.8439754781551088 1105 66-67 0.777928605135692 1105 68-69 0.704508975850537 1105 70-71 0.7548148144454245 1105 72-73 0.6093295233779656 1105 74-75 0.5549988002060857 1105 76-77 0.5725246921029097 1105 78-79 0.6241254873576949 1105 80-81 0.6657707672419377 1105 82-83 0.7433308309242292 1105 84-85 0.5564955630792667 1105 86-87 0.6030542693968499 1105 88-89 0.5546073546601988 1105 90-91 0.5408591111098389 1105 92-93 0.4260018471425404 1105 94-95 0.5404392912429223 1105 96-97 0.5302396302705326 1105 98-99 0.6551930589608297 1105 100-101 0.667265491509859 1106 1 0.10806253826486767 1106 2 0.16651528569340002 1106 3 0.08351264905309108 1106 4 0.09839517697547961 1106 5 0.11421113485854306 1106 6 0.04645224463663311 1106 7 -0.052776824100078557 1106 8 -0.006388362492096178 1106 9 0.13916174437603246 1106 10-11 0.21634740829173893 1106 12-13 0.22536164464164443 1106 14-15 0.18553154310340858 1106 16-17 0.272316568005472 1106 18-19 0.273843528611728 1106 20-21 0.21960434152175878 1106 22-23 0.1372886240343334 1106 24-25 0.17254022091752574 1106 26-27 0.29167162862086826 1106 28-29 0.22292476629969116 1106 30-31 0.05845356748563546 1106 32-33 0.08922475695702303 1106 34-35 0.05744864120254789 1106 36-37 0.1200051254503407 1106 38-39 0.3094175517719435 1106 40-41 0.1896598481662437 1106 42-43 0.09432262585092843 1106 44-45 0.160926413254181 1106 46-47 0.17792523816418537 1106 48-49 -0.05410258966350767 1106 50-51 -0.045151660190775544 1106 52-53 -0.09959695397299129 1106 54-55 -0.141636586315947 1106 56-57 0.03247158453657306 1106 58-59 0.16828515611729955 1106 60-61 0.1705865524511978 1106 62-63 0.21809993256393767 1106 64-65 0.18580774432160752 1106 66-67 0.018058659356391615 1106 68-69 -0.046839130736792356 1106 70-71 0.03712490725659734 1106 72-73 0.2875679435398766 1106 74-75 0.30051723847748946 1106 76-77 0.19030649421352308 1106 78-79 0.2710259875247871 1106 80-81 0.26246928722166984 1106 82-83 0.2792230740226742 1106 84-85 0.0536881866927601 1106 86-87 0.10142791792168282 1106 88-89 0.07051851571615941 1106 90-91 0.2823473776644079 1106 92-93 0.0920911388240242 1106 94-95 0.11430084521029826 1106 96-97 0.1553575306467394 1106 98-99 0.12147489879957263 1106 100-101 0.3128892990317702 1107 1 0.0038672404824353634 1107 2 0.06606760486084795 1107 3 0.10184660364241438 1107 4 0.15749607522378994 1107 5 0.10249512540541161 1107 6 0.07600018363556416 1107 7 0.002971801920125472 1107 8 -0.06981044130250069 1107 9 0.1221154840233396 1107 10-11 0.13129432541870045 1107 12-13 0.017914228627297746 1107 14-15 0.18427459790070344 1107 16-17 -0.04924302299472316 1107 18-19 0.01775678872471076 1107 20-21 0.13007696044835626 1107 22-23 0.1404670700911126 1107 24-25 0.07763364150281404 1107 26-27 0.04711326632696711 1107 28-29 0.09854743423648671 1107 30-31 0.13415580639182423 1107 32-33 0.08595845651761636 1107 34-35 0.06931400310612901 1107 36-37 0.21555743457728482 1107 38-39 0.1606938876919557 1107 40-41 0.2057070786823374 1107 42-43 0.21276915955625242 1107 44-45 0.2254491819725004 1107 46-47 0.28614426667042636 1107 48-49 0.1391889751862081 1107 50-51 0.10578844445634417 1107 52-53 0.01989567441677309 1107 54-55 0.1514293182767048 1107 56-57 0.151634758991527 1107 58-59 0.07521598836186882 1107 60-61 0.29132169282367926 1107 62-63 0.023269660955840266 1107 64-65 0.0646916924473615 1107 66-67 0.08217667038336884 1107 68-69 -0.09229436077094277 1107 70-71 -0.056584969960624676 1107 72-73 0.021591937826833885 1107 74-75 0.1480804716693811 1107 76-77 -0.007962021411373144 1107 78-79 -0.12779337507912203 1107 80-81 -0.13507304917777674 1107 82-83 -0.0993370679500103 1107 84-85 -0.0714440655296471 1107 86-87 0.09545381990380264 1107 88-89 0.15526850096917855 1107 90-91 0.15608619662538104 1107 92-93 0.06997881825091312 1107 94-95 0.015450859078740109 1107 96-97 0.01041223255054291 1107 98-99 -0.023332969940959458 1107 100-101 0.1505099262367544 1108 1 0.06799126455008064 1108 2 0.039418907175459594 1108 3 0.12626425864273472 1108 4 0.10577493412998251 1108 5 0.1158301889675073 1108 6 0.087801851798325 1108 7 0.11405010747058952 1108 8 0.26657936495156065 1108 9 0.41701313464253076 1108 10-11 0.28077894741530685 1108 12-13 0.25852575337949446 1108 14-15 0.3464305661184639 1108 16-17 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-0.8627469117985598 2316 84-85 -0.8637489455224383 2316 86-87 -0.9038955490447229 2316 88-89 -0.9496637329045328 2316 90-91 -0.6784061725379971 2316 92-93 -0.6162848961159888 2316 94-95 -0.6906198491821094 2316 96-97 -0.7224465415254038 2316 98-99 -0.5735966011379823 2316 100-101 -0.5420326810004106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 34.0 7 775.0 8 1819.0 9 1460.0 10 1475.0 11 1634.0 12 1840.0 13 1922.0 14 1845.0 15 1859.0 16 2081.0 17 2499.0 18 3313.0 19 4185.0 20 5204.0 21 6841.0 22 7545.0 23 7803.0 24 6964.0 25 6291.0 26 6432.0 27 6662.0 28 7559.0 29 8688.0 30 10555.0 31 12898.0 32 16427.0 33 22014.0 34 32241.0 35 52502.0 36 101196.0 37 211834.0 38 289076.0 39 91298.0 40 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.556161833521195 19.264375619086653 13.673382689483212 23.506079857908937 2 15.443970522936093 21.366068073914676 39.75112716466851 23.43883423848072 3 17.592414694128497 26.80805324999146 30.89765430201182 24.701877753868224 4 12.48388239915292 18.08512228028828 38.74425743757899 30.686737882979813 5 13.204371178740992 37.89696263278341 35.09997182088328 13.798694367592306 6 29.586791679475354 37.89312002595894 18.629491580421494 13.890596714144209 7 25.98616234586877 33.54830583734672 23.257164326946068 17.208367489838437 8 25.39888393619565 34.60907879905728 21.46159954913413 18.530437715612937 9 26.766638487549955 15.378325989684734 20.696066878436998 37.15896864432831 10-11 23.979147453632542 26.23262287802712 29.46671021621068 20.321519452129657 12-13 25.279816494176316 24.463262543976498 28.891653431020934 21.365267530826245 14-15 22.70190764081019 25.581461129897185 26.803943795470847 24.91268743382177 16-17 21.80380503466885 28.52046828568501 27.395811985517643 22.279914694128497 18-19 21.85536000956382 27.476399989753048 29.450005550432078 21.21823445025105 20-21 22.824817689653994 26.801435427127096 29.175632749257097 21.198114133961813 22-23 22.73110077876832 26.716737968371074 28.648074853980944 21.904086398879667 24-25 22.329441626532773 26.82844041397684 28.845488779588074 21.996629179902314 26-27 21.9928933121563 27.13029852785463 28.900406035454452 21.976402124534616 28-29 22.08319457253134 27.003919458960958 29.10518495747515 21.807701011032552 30-31 22.421183864466986 26.897714075895756 28.87580267787 21.805299381767256 32-33 22.203382774874473 27.041118027803396 28.672624842026163 22.082874355295967 34-35 22.492912525190423 27.125388530245587 28.684152662499574 21.69754628206442 36-37 22.547242716125286 27.01614108344434 28.55846739761588 21.878148802814497 38-39 22.326879888649792 27.11236636267377 28.615145848276804 21.94560790039963 40-41 22.314017829695665 27.043466287529462 28.69322548416846 21.949290398606415 42-43 22.31764695836322 27.054673890767493 28.46202864022953 22.16565051063975 44-45 22.31417793831335 27.217771202650543 28.2789177511357 22.1891331079004 46-47 22.307411912194254 27.19713170595289 28.08520131267789 22.410255069174966 48-49 22.210872263868385 27.371990081773067 27.919563307683852 22.497574346674693 50-51 22.30446468217372 27.480669552891346 27.729158127540387 22.48570763739454 52-53 22.331843255798066 27.48792781022646 27.679898042832257 22.500330891143218 54-55 22.22056776650613 27.368806998667893 27.755629418997845 22.654995815828123 56-57 22.2023153840899 27.45249043617857 27.688116951873482 22.657077227858046 58-59 22.180700720702255 27.343456467534246 27.747623988113535 22.728218823649964 60-61 22.20962701096424 27.32349625986269 27.842568398401475 22.624308330771594 62-63 22.175790723093215 27.142947108651843 27.953203453222663 22.72805871503228 64-65 22.131867592307955 27.075221163370568 27.981275830856987 22.811635413464497 66-67 22.106677169791986 27.153087321105307 27.756429962086283 22.983805547016427 68-69 22.187318543566622 27.25288835946306 27.688704016804998 22.87108908016532 70-71 22.188759521125796 27.01864945178809 27.607528947638077 23.18506207944803 72-73 22.18144789425146 27.097369522150494 27.59968362537145 23.121498958226596 74-75 22.097444239505414 27.175822744816752 27.58484689346586 23.141886122211975 76-77 22.16063377395225 27.02505379649554 27.553892560713187 23.26041986883902 78-79 22.20701190354203 27.074740837517503 27.528061703726475 23.19018555521399 80-81 22.217312224613178 27.123573965911806 27.45932173719985 23.199792072275166 82-83 22.189293216518085 26.96298502237251 27.49753432728763 23.35018743382177 84-85 22.36189030638385 26.97408588653209 27.35461070123305 23.30941310585101 86-87 22.349455203743553 26.77330967995355 27.546420825221162 23.330814291081737 88-89 22.365893021826004 26.754310123988112 27.45841445503296 23.421382399152918 90-91 22.530644789425146 26.60636976124603 27.50111008641596 23.36187536291287 92-93 22.560424992314786 26.52807664719746 27.503458346142022 23.408040014345733 94-95 22.526375226286845 26.334985654267857 27.626635242681967 23.51200387676333 96-97 22.550178040782868 26.282630136284457 27.579563309082218 23.58762851385046 98-99 22.591219216449772 26.32719370154046 27.433971206066193 23.647615875943572 100-101 22.913677972469856 26.152194982409398 27.525126379068894 23.40900066605185 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 161.0 1 116.5 2 112.5 3 146.5 4 120.0 5 129.0 6 194.5 7 362.5 8 694.5 9 884.5 10 760.0 11 637.5 12 690.5 13 778.0 14 835.5 15 911.0 16 990.0 17 1049.5 18 1149.5 19 1298.5 20 1510.0 21 1803.5 22 2151.5 23 2686.0 24 3275.5 25 3946.0 26 4990.5 27 6337.5 28 7778.0 29 9397.5 30 11213.0 31 13195.0 32 15172.5 33 16951.5 34 18680.0 35 20570.0 36 22666.5 37 24422.0 38 25565.0 39 26668.0 40 28081.5 41 29346.5 42 30111.0 43 31525.5 44 33375.0 45 34788.0 46 35594.0 47 36204.0 48 36932.0 49 37225.5 50 36630.0 51 35527.0 52 34417.5 53 33480.5 54 31860.0 55 29105.5 56 25739.5 57 22128.0 58 19852.0 59 17753.5 60 14897.0 61 12159.0 62 9869.5 63 7696.0 64 5540.5 65 3944.0 66 2903.0 67 2236.0 68 1717.0 69 1274.5 70 918.5 71 686.5 72 544.5 73 449.0 74 354.0 75 264.5 76 204.5 77 138.0 78 94.0 79 78.5 80 64.0 81 51.5 82 38.5 83 31.5 84 25.5 85 18.5 86 15.5 87 12.5 88 11.5 89 7.5 90 5.5 91 4.5 92 2.0 93 2.5 94 3.0 95 1.5 96 0.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.336953922874612E-5 48-49 3.202172353724767E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 936864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.54742349615864 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55410822034804 42.60862785087032 2 15.304289661176696 18.226620355239422 3 6.028034074354884 10.76861693624655 4 2.7490168509470494 6.547874824856816 5 1.5424977115640814 4.592588223618097 6 0.8704006463328393 3.1098069539107076 7 0.5176802700563063 2.1578568393654054 8 0.3734192932561081 1.7788925437726186 9 0.24583463263130154 1.3174937081386828 >10 0.7962820068844301 7.140493156725046 >50 0.013557524956358903 0.5410906470661236 >100 0.0043369845411703935 0.4132099782154886 >500 0.0 0.0 >1k 5.421229506288122E-4 0.7968279819747992 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3289 0.35106482904669195 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2629 0.28061703726474707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.15872100966629094 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0673907845749223E-4 0.0 5 0.0 0.0 0.0 1.0673907845749223E-4 0.0 6 0.0 0.0 0.0 1.0673907845749223E-4 0.0 7 0.0 0.0 0.0 1.0673907845749223E-4 0.0 8 0.0 0.0 0.0 1.0673907845749223E-4 0.0 9 0.0 0.0 0.0 1.0673907845749223E-4 0.0 10-11 0.0 0.0 0.0 2.1347815691498447E-4 0.0 12-13 0.0 0.0 0.0 3.202172353724767E-4 0.0 14-15 0.0 0.0 0.0 3.735867746012228E-4 0.0 16-17 0.0 0.0 0.0 4.80325853058715E-4 0.0 18-19 0.0 0.0 0.0 8.539126276599379E-4 0.0 20-21 0.0 0.0 0.0 0.0010673907845749223 0.0 22-23 0.0 0.0 0.0 0.00192130341223486 0.0 24-25 0.0 0.0 0.0 0.0035757591283259896 0.0 26-27 0.0 0.0 0.0 0.006777931482050757 0.0 28-29 0.0 0.0 0.0 0.016918143935512518 0.0 30-31 0.0 0.0 0.0 0.0438697612460293 0.0 32-33 0.0 0.0 0.0 0.10054821190695767 0.0 34-35 0.0 0.0 0.0 0.18364458448611537 0.0 36-37 0.0 0.0 0.0 0.32859625303138984 0.0 38-39 0.0 0.0 0.0 0.5204597465587322 0.0 40-41 0.0 0.0 0.0 0.7101350889776958 0.0 42-43 0.0 0.0 0.0 0.8810243535881409 0.0 44-45 0.0 0.0 0.0 1.0856965365303823 0.0 46-47 0.0 0.0 0.0 1.3412298903576185 0.0 48-49 0.0 0.0 0.0 1.6085045428151792 0.0 50-51 0.0 0.0 0.0 1.864785070191618 0.0 52-53 0.0 0.0 0.0 2.113060166683745 0.0 54-55 0.0 0.0 0.0 2.3739838439730847 0.0 56-57 0.0 0.0 0.0 2.6762155446254736 0.0 58-59 0.0 0.0 0.0 2.9886408272705536 0.0 60-61 0.0 0.0 0.0 3.3124338217713563 0.0 62-63 0.0 0.0 0.0 3.644659203470301 0.0 64-65 0.0 0.0 0.0 3.9910275130648634 0.0 66-67 0.0 0.0 0.0 4.389057536632851 0.0 68-69 0.0 0.0 0.0 4.849049595245415 0.0 70-71 0.0 0.0 0.0 5.299435136796803 0.0 72-73 0.0 0.0 0.0 5.7418152474638795 0.0 74-75 0.0 0.0 0.0 6.182060576561806 0.0 76-77 0.0 0.0 0.0 6.648030023567989 0.0 78-79 0.0 0.0 0.0 7.158883253065547 0.0 80-81 0.0 0.0 0.0 7.699997011305803 0.0 82-83 0.0 0.0 0.0 8.24105740000683 0.0 84-85 0.0 0.0 0.0 8.800957236055606 0.0 86-87 0.0 0.0 0.0 9.407021723537248 0.0 88-89 0.0 0.0 0.0 10.071472486935136 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1655 0.0 57.401814 1 GTATCAA 2980 0.0 46.065437 1 TCAACGC 3765 0.0 36.2085 4 CAACGCA 3805 0.0 35.703022 5 TATCAAC 3895 0.0 35.121952 2 ATCAACG 3885 0.0 34.967823 3 AACGCAG 3940 0.0 34.479694 6 ACGCAGA 4320 0.0 31.116898 7 CGCAGAG 4555 0.0 29.094402 8 GTACATG 2980 0.0 26.93792 1 TACATGG 2985 0.0 26.256283 2 GCAGAGT 4905 0.0 25.856272 9 GAGTACT 2890 0.0 24.1609 12-13 ACATGGG 3240 0.0 23.310184 3 CAGAGTA 4740 0.0 22.29694 10-11 AGAGTAC 4430 0.0 22.19526 10-11 GTATAGA 290 1.05501385E-10 21.293106 1 GTACTTT 3390 0.0 20.737465 14-15 CGTATCA 195 0.0 20.70513 94-95 GGCGACG 140 4.8508155E-4 20.357143 8 >>END_MODULE