FastQCFastQC Report
Fri 10 Feb 2017
SRR2031502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936864
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT63430.6770459746558732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39220.4186306657102845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.36472743108925093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.20387163985381018No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT18560.1981077296171056RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT12610.1345979779348977RNA PCR Primer, Index 23 (96% over 25bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG10690.11410407487105918RNA PCR Primer, Index 23 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.062.5206341
GTATCAA31450.042.5029681
TCAACGC38250.034.64634
ATCAACG38950.034.0236433
CAACGCA39200.033.8066565
AACGCAG39550.033.747686
TATCAAC39800.033.4163552
ACGCAGA43800.030.3646327
CGCAGAG45500.029.0213438
GCAGAGT50600.025.6269079
GAGTACT32700.024.69364712-13
CCGTCCG1000.001653629223.749489
GTACATG29000.023.6209871
CAGAGTA49000.023.41020210-11
TACATGG29650.022.2676412
GTACTTT36550.022.09253914-15
GTATGGA3251.8189894E-1221.9554061
AGAGTAC46200.021.8988710-11
ACATGGG31100.021.2294373
AGTACTT35350.019.8864112-13
Produced by FastQC (version 0.11.5)