##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031502_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 936864 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79193031219046 34.0 31.0 34.0 31.0 34.0 2 32.94150378283294 34.0 33.0 34.0 31.0 34.0 3 33.04429778495064 34.0 33.0 34.0 31.0 34.0 4 36.40985884824265 37.0 37.0 37.0 35.0 37.0 5 36.32910860060799 37.0 37.0 37.0 35.0 37.0 6 36.34492412986303 37.0 37.0 37.0 35.0 37.0 7 36.367612588379956 37.0 37.0 37.0 35.0 37.0 8 36.39545227482324 37.0 37.0 37.0 35.0 37.0 9 38.270820524643916 39.0 39.0 39.0 37.0 39.0 10-11 38.235466407077226 39.0 39.0 39.0 37.0 39.0 12-13 38.241373347679065 39.0 39.0 39.0 37.0 39.0 14-15 39.80753663285173 41.0 40.0 41.0 37.5 41.0 16-17 39.794101918741674 41.0 40.0 41.0 37.5 41.0 18-19 39.802711492810054 41.0 40.0 41.0 38.0 41.0 20-21 39.790618488916216 41.0 40.0 41.0 37.5 41.0 22-23 39.76190354202958 41.0 40.0 41.0 37.0 41.0 24-25 39.71429684564676 41.0 40.0 41.0 37.0 41.0 26-27 39.60402950695085 41.0 40.0 41.0 37.0 41.0 28-29 39.510314197151345 41.0 40.0 41.0 37.0 41.0 30-31 39.37621575810363 41.0 39.0 41.0 36.0 41.0 32-33 38.44411942395054 40.0 38.0 41.0 35.0 41.0 34-35 38.625603075793286 40.0 38.0 41.0 35.0 41.0 36-37 38.83239402944291 40.0 38.0 41.0 35.0 41.0 38-39 38.768921636438165 40.0 38.0 41.0 35.0 41.0 40-41 38.67964667230249 40.0 38.0 41.0 35.0 41.0 42-43 38.493303190217574 40.0 38.0 41.0 35.0 41.0 44-45 38.32611456945725 40.0 38.0 41.0 34.0 41.0 46-47 38.07952595040476 40.0 38.0 41.0 33.5 41.0 48-49 37.91255507736449 40.0 38.0 41.0 33.0 41.0 50-51 37.88826233049834 40.0 38.0 41.0 33.0 41.0 52-53 37.98576847866926 40.0 38.0 41.0 34.0 41.0 54-55 37.87925835553506 40.0 38.0 41.0 34.0 41.0 56-57 37.68112554223452 40.0 37.0 41.0 33.0 41.0 58-59 37.49528800338149 40.0 37.0 41.0 33.0 41.0 60-61 37.24353321293165 40.0 36.0 41.0 33.0 41.0 62-63 36.91348584212864 39.0 35.5 41.0 32.0 41.0 64-65 36.620585271715 39.0 35.0 41.0 32.0 41.0 66-67 36.26790814888821 38.5 35.0 41.0 31.0 41.0 68-69 35.84994300133211 37.0 35.0 40.0 31.0 41.0 70-71 35.39640118523073 37.0 35.0 39.5 30.0 41.0 72-73 34.99305181968781 36.0 35.0 39.0 30.0 41.0 74-75 34.5809274345049 36.0 35.0 39.0 29.0 40.5 76-77 33.16118561498787 35.0 33.0 36.5 27.0 39.0 78-79 33.82841853246576 35.0 34.0 37.0 29.0 39.0 80-81 33.68335959114663 35.0 34.0 37.0 29.0 39.0 82-83 33.441957423916385 35.0 34.0 36.0 29.0 38.0 84-85 33.191719929466814 35.0 34.0 36.0 29.0 37.0 86-87 32.98458367489839 35.0 34.0 35.5 28.5 37.0 88-89 32.80600546077125 35.0 34.0 35.0 27.5 36.0 90-91 32.66011982528948 35.0 34.0 35.0 27.0 36.0 92-93 32.53795001195478 35.0 34.0 35.0 27.0 36.0 94-95 32.43617323325478 35.0 34.0 35.0 27.0 36.0 96-97 32.35722954452301 35.0 34.0 35.0 26.0 35.0 98-99 32.29973667469345 35.0 34.0 35.0 25.5 35.0 100-101 31.125105671687674 34.5 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36297799983964296 1101 2 0.3929616745383484 1101 3 0.3532070185918599 1101 4 0.15564022680582212 1101 5 0.22913619108198446 1101 6 0.27199133114133645 1101 7 0.26856340114601807 1101 8 0.24761153586567985 1101 9 0.25558157586375785 1101 10-11 0.3748763198298093 1101 12-13 0.3338404874600087 1101 14-15 0.3930778005146962 1101 16-17 0.27740656082413295 1101 18-19 0.28339177779160707 1101 20-21 0.22571197162259438 1101 22-23 0.48394194427490334 1101 24-25 0.3628699885731308 1101 26-27 0.38769439636957514 1101 28-29 0.4479420797482305 1101 30-31 0.29170498673323664 1101 32-33 0.42347792688939023 1101 34-35 0.2176803284815989 1101 36-37 0.2935056059443255 1101 38-39 0.4545761206693868 1101 40-41 0.6428230040156251 1101 42-43 0.632785322845713 1101 44-45 0.6531780168953603 1101 46-47 0.5871696119417322 1101 48-49 0.5533260875762593 1101 50-51 0.7454249005421403 1101 52-53 0.6324452077381721 1101 54-55 0.8860989838575222 1101 56-57 0.810163167146051 1101 58-59 0.8719046238093355 1101 60-61 0.6224018167402292 1101 62-63 0.7583153622083003 1101 64-65 0.8174837354207227 1101 66-67 0.6342111672954189 1101 68-69 0.45100482211200443 1101 70-71 0.4070295113651028 1101 72-73 0.49639008082403535 1101 74-75 0.4333631835676073 1101 76-77 0.4997401757025699 1101 78-79 0.6073355865216072 1101 80-81 0.602863270297135 1101 82-83 0.6045087212514986 1101 84-85 0.6137152326580377 1101 86-87 0.7487951744215806 1101 88-89 0.6514296431362254 1101 90-91 0.5985342921849934 1101 92-93 0.5653702630164261 1101 94-95 0.39709590967049024 1101 96-97 0.6093281333836629 1101 98-99 0.4465111176759038 1101 100-101 0.5569053932379191 1102 1 0.14338171222964036 1102 2 0.2500653956146479 1102 3 0.08642408917136635 1102 4 0.02957532011670594 1102 5 -0.003676632566126159 1102 6 0.11415462017455269 1102 7 0.05296281047777285 1102 8 0.010128156762235108 1102 9 0.11308620298772354 1102 10-11 0.1439277093033553 1102 12-13 0.2283353792487901 1102 14-15 0.32127552737740217 1102 16-17 0.27552230240009123 1102 18-19 0.2659824326716347 1102 20-21 0.31834799570368943 1102 22-23 0.27471241888084563 1102 24-25 0.2919630722756281 1102 26-27 0.24572688312296265 1102 28-29 0.2791606336801209 1102 30-31 0.3290994983294411 1102 32-33 0.19688248786385998 1102 34-35 0.1785919888339933 1102 36-37 0.19269443937454867 1102 38-39 0.32504337666794925 1102 40-41 0.4241655047339137 1102 42-43 0.5784324854331615 1102 44-45 0.5139166194705069 1102 46-47 0.5033869297466396 1102 48-49 0.4040320163085269 1102 50-51 0.41032758059907337 1102 52-53 0.5158416837740134 1102 54-55 0.4454932148194928 1102 56-57 0.3820021900043784 1102 58-59 0.3898819191634928 1102 60-61 0.4144752360010884 1102 62-63 0.38784142444228564 1102 64-65 0.4707133677230573 1102 66-67 0.3005003141005318 1102 68-69 0.18692132674581075 1102 70-71 0.1820343556465147 1102 72-73 0.311328508336878 1102 74-75 0.33945094190143266 1102 76-77 0.23563329415026146 1102 78-79 0.22150694712991736 1102 80-81 0.2947469273621053 1102 82-83 0.1702740174876638 1102 84-85 0.3149500232848581 1102 86-87 0.4688925945911606 1102 88-89 0.4956542574311982 1102 90-91 0.5131145769619252 1102 92-93 0.4324899535169564 1102 94-95 0.5213901479824017 1102 96-97 0.5300990757133306 1102 98-99 0.548815659273238 1102 100-101 0.4263536796986571 1103 1 0.43160291357325065 1103 2 0.3299091588460783 1103 3 0.28441573396206365 1103 4 0.19018375436176882 1103 5 0.2423945137347161 1103 6 0.2859356769956207 1103 7 0.23198234360222614 1103 8 0.13537690771489963 1103 9 0.14725152886911275 1103 10-11 0.22342745525983787 1103 12-13 0.26581738449902304 1103 14-15 0.241781956089973 1103 16-17 0.2653385089657121 1103 18-19 0.26270632969932706 1103 20-21 0.20347886340346832 1103 22-23 0.17044166493611357 1103 24-25 0.16783811108783198 1103 26-27 0.19365766331886647 1103 28-29 0.1841024859396967 1103 30-31 0.15764393477622463 1103 32-33 0.3955974510275482 1103 34-35 0.2640423515517014 1103 36-37 0.23496975198921888 1103 38-39 0.3311104300453138 1103 40-41 0.2950205376184343 1103 42-43 0.4382083978728275 1103 44-45 0.5703971992542876 1103 46-47 0.38234136421752396 1103 48-49 0.39706787172953995 1103 50-51 0.24107460970116534 1103 52-53 0.3125971598233548 1103 54-55 0.3752140139275184 1103 56-57 0.5257326074825741 1103 58-59 0.5796515510826552 1103 60-61 0.6260352749544253 1103 62-63 0.7052255876511353 1103 64-65 0.5958633239711162 1103 66-67 0.6049370784328261 1103 68-69 0.534388477506532 1103 70-71 0.44043719811130444 1103 72-73 0.4145345729203882 1103 74-75 0.445449721081161 1103 76-77 0.43254790024165857 1103 78-79 0.6271932880563256 1103 80-81 0.4767602928742818 1103 82-83 0.3821807200026939 1103 84-85 0.45485567434196383 1103 86-87 0.32042708805659714 1103 88-89 0.4264212218933281 1103 90-91 0.4985864628117014 1103 92-93 0.5452270608080099 1103 94-95 0.4873439050038826 1103 96-97 0.4869346107572241 1103 98-99 0.53630313351605 1103 100-101 0.5394497998894714 1104 1 0.2877537072240486 1104 2 0.2994931462872117 1104 3 0.22829328498247037 1104 4 0.11916945120461264 1104 5 0.2263579709124599 1104 6 0.2967829646286262 1104 7 0.15508107027238083 1104 8 0.1736967228204307 1104 9 0.2863366500970912 1104 10-11 0.2244631259383567 1104 12-13 0.22210118006853463 1104 14-15 0.2111751620903206 1104 16-17 0.20149213708361913 1104 18-19 0.26321871414028664 1104 20-21 0.1990799884688741 1104 22-23 0.21230456394187058 1104 24-25 0.2616153655793738 1104 26-27 0.15852662372353876 1104 28-29 0.25542203940210584 1104 30-31 0.25289838270924037 1104 32-33 0.66140505612087 1104 34-35 0.49659947696597584 1104 36-37 0.5053670134579136 1104 38-39 0.41133675134305747 1104 40-41 0.32816745022963545 1104 42-43 0.3809140057570062 1104 44-45 0.4829102814102342 1104 46-47 0.36297432252905537 1104 48-49 0.38364230919682996 1104 50-51 0.36856021932963756 1104 52-53 0.33656475968381727 1104 54-55 0.4027683048746695 1104 56-57 0.3675148331933613 1104 58-59 0.47730003355255946 1104 60-61 0.6000344900250738 1104 62-63 0.520282058957612 1104 64-65 0.3307643356578396 1104 66-67 0.3534162778048824 1104 68-69 0.26733261670067066 1104 70-71 0.20512191815316072 1104 72-73 0.3517456753100632 1104 74-75 0.4084653760611019 1104 76-77 0.3383291229871688 1104 78-79 0.35757005414738074 1104 80-81 0.29259624263880113 1104 82-83 0.2267592565455132 1104 84-85 0.2021847341710199 1104 86-87 0.23082522166901498 1104 88-89 0.3523816701752054 1104 90-91 0.23786999673808396 1104 92-93 0.2461580742390126 1104 94-95 0.2021692582220922 1104 96-97 0.31421744696862675 1104 98-99 0.24988568192002703 1104 100-101 0.12874096507349364 1105 1 0.2819867587000857 1105 2 0.1682412206427628 1105 3 0.13533600861398298 1105 4 0.10778817930931694 1105 5 0.07017679487147177 1105 6 0.13939505139958897 1105 7 0.14183834207638313 1105 8 0.14320877878108007 1105 9 0.16472721073063923 1105 10-11 0.25642383138037417 1105 12-13 0.13771801390966232 1105 14-15 0.1380662875795693 1105 16-17 0.16517519597695696 1105 18-19 0.2500465929074096 1105 20-21 0.29023143826518805 1105 22-23 0.16772375534237227 1105 24-25 0.13956231478800163 1105 26-27 0.26411470127423087 1105 28-29 0.2604900503112404 1105 30-31 0.20894447835814134 1105 32-33 0.6908959442566172 1105 34-35 0.5609858953243503 1105 36-37 0.27850398811357735 1105 38-39 0.4259630907357561 1105 40-41 0.30066496828790434 1105 42-43 0.3181971924560827 1105 44-45 0.08662121222396735 1105 46-47 0.09010051928142104 1105 48-49 0.11009595679534101 1105 50-51 0.17568981092184544 1105 52-53 0.14687023821358736 1105 54-55 0.07388962903337415 1105 56-57 0.13773909231949233 1105 58-59 0.16336435863558307 1105 60-61 -0.03795719119090535 1105 62-63 0.11498026054832877 1105 64-65 0.14875527514411147 1105 66-67 0.015228180855103801 1105 68-69 0.08900296348784309 1105 70-71 0.05939342546205495 1105 72-73 0.1187056104942954 1105 74-75 0.07484248285877015 1105 76-77 0.24668699045718512 1105 78-79 0.11477196438666937 1105 80-81 0.22144368024792271 1105 82-83 0.27083583158003677 1105 84-85 0.22796982654489995 1105 86-87 0.20370002339895876 1105 88-89 0.21816139927268807 1105 90-91 0.15115894040086175 1105 92-93 0.2787884760282964 1105 94-95 0.25895009420853654 1105 96-97 0.257819600267041 1105 98-99 0.13461874480729108 1105 100-101 0.184804320202268 1106 1 0.2095420303964488 1106 2 0.18861440565068222 1106 3 0.09367473167879581 1106 4 0.10231625442062153 1106 5 0.0823643251887134 1106 6 0.24432638622048586 1106 7 0.16767132507717264 1106 8 0.17096320105576268 1106 9 0.15442204413404426 1106 10-11 0.1738443867383097 1106 12-13 0.09349257222116591 1106 14-15 0.19323827391121284 1106 16-17 0.0829662953743906 1106 18-19 0.1129890898622179 1106 20-21 0.26123064058492673 1106 22-23 0.2577942530301769 1106 24-25 0.1999421291656489 1106 26-27 0.17839641175941523 1106 28-29 0.1483499496310543 1106 30-31 0.12106827510058338 1106 32-33 0.6105687336582477 1106 34-35 0.4025506960659797 1106 36-37 0.24761745813049885 1106 38-39 0.27863208576641796 1106 40-41 0.45884415530080247 1106 42-43 0.4225484574260605 1106 44-45 0.392704063176339 1106 46-47 0.34335591709749735 1106 48-49 0.374275666612192 1106 50-51 0.43995710387530806 1106 52-53 0.4916298022921026 1106 54-55 0.45242798775234405 1106 56-57 0.30582258464990986 1106 58-59 0.2060793280758091 1106 60-61 0.1736557937501786 1106 62-63 0.2485142266361393 1106 64-65 0.0867140760366425 1106 66-67 0.2033345256470085 1106 68-69 0.14947642445001463 1106 70-71 0.2216688084024767 1106 72-73 0.22240404423832416 1106 74-75 0.1927199527032144 1106 76-77 0.028207487606017878 1106 78-79 0.11730109588533821 1106 80-81 0.11494056498312233 1106 82-83 0.0738404721388406 1106 84-85 0.14911131777580522 1106 86-87 0.0655295408024088 1106 88-89 -0.0014742009817041435 1106 90-91 0.07956444223876247 1106 92-93 0.13328728489764075 1106 94-95 0.11606966001176744 1106 96-97 0.1339507701621585 1106 98-99 0.15494544338117322 1106 100-101 0.192942814742473 1107 1 0.24429722415928978 1107 2 0.22846515360524222 1107 3 0.1667356913247673 1107 4 0.16448201555961361 1107 5 -0.01185950016681403 1107 6 0.1853396986695799 1107 7 0.0905991853334811 1107 8 0.0518025367260222 1107 9 0.06977434648364067 1107 10-11 0.048532660562841556 1107 12-13 0.12697136731085834 1107 14-15 0.07213979291348949 1107 16-17 0.15134273383301888 1107 18-19 0.12953834542182818 1107 20-21 0.10954565627948654 1107 22-23 0.10231082173920925 1107 24-25 0.08757951145646103 1107 26-27 0.11859895376321816 1107 28-29 0.14844094167019506 1107 30-31 0.12155837704932537 1107 32-33 0.5803951459488204 1107 34-35 0.24251829034941608 1107 36-37 0.0387618618690837 1107 38-39 -0.06294449878149777 1107 40-41 -0.04121797029833374 1107 42-43 -0.13088081289308917 1107 44-45 -0.280076640989094 1107 46-47 -0.19177077519754704 1107 48-49 -0.21429221909678375 1107 50-51 -0.1350190813116896 1107 52-53 -0.03661851937541627 1107 54-55 0.0010974331276258908 1107 56-57 -0.10067336840071306 1107 58-59 -0.019182446607821646 1107 60-61 0.018627322654900524 1107 62-63 0.1028384821016246 1107 64-65 -0.05999458502490995 1107 66-67 -0.2824217688057473 1107 68-69 -0.19775923740098023 1107 70-71 -0.11187537544913795 1107 72-73 -0.23398880541898137 1107 74-75 -0.22893778419298627 1107 76-77 -0.23338709453971518 1107 78-79 -0.04235102586076067 1107 80-81 -0.15636882928600215 1107 82-83 -0.03276329078987317 1107 84-85 -0.09454779215889886 1107 86-87 -0.19089983117681442 1107 88-89 -0.057060296394070065 1107 90-91 0.013552541735109003 1107 92-93 0.09010309065411093 1107 94-95 0.06187103631342694 1107 96-97 0.18057760114162136 1107 98-99 0.16461821090718587 1107 100-101 -0.0738728278558618 1108 1 0.12086127556137427 1108 2 0.10145048872595197 1108 3 0.16686783953076656 1108 4 0.08230964191591994 1108 5 0.09711752985582933 1108 6 0.06445742486325656 1108 7 -0.005363032184845906 1108 8 -0.035460519159570936 1108 9 0.017004353412495732 1108 10-11 0.07643676620521944 1108 12-13 0.09515579382336625 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-0.6826511490932887 2316 90-91 -0.6242443574144829 2316 92-93 -0.6322069701292108 2316 94-95 -0.6637796813733416 2316 96-97 -0.6460027998758235 2316 98-99 -0.6761586824205139 2316 100-101 -0.6187256518198758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 1.0 12 2.0 13 10.0 14 43.0 15 159.0 16 483.0 17 1010.0 18 1738.0 19 2736.0 20 4000.0 21 5441.0 22 7015.0 23 7402.0 24 6457.0 25 6313.0 26 6675.0 27 7382.0 28 8858.0 29 10123.0 30 9473.0 31 11193.0 32 13876.0 33 18508.0 34 26455.0 35 43680.0 36 87300.0 37 214715.0 38 334865.0 39 100872.0 40 77.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.76894397721977 19.33390910995481 13.957915574615093 23.93923133821033 2 15.18747651740274 21.1676401270622 40.289519076408105 23.35536427912696 3 17.044309526249275 26.63609659459644 31.99002203094579 24.32957184820849 4 12.234966868190046 18.824183659527957 38.3427050244219 30.598144447860093 5 12.765139870888412 37.70995576732589 35.811601256959385 13.713303104826313 6 29.09237626806025 38.37547392150835 18.470770570755203 14.061379239676198 7 25.35213221983127 33.56303583017385 23.14220633944735 17.94262561054753 8 24.99327543805718 34.90293148205076 21.703256822761897 18.40053625713017 9 26.112434675683982 15.102191993715202 20.58484475868429 38.20052857191652 10-11 23.47843443658845 26.180267360043718 29.83554710182054 20.50575110154729 12-13 24.88520212111897 24.16498018922704 29.290537367216587 21.659280322437407 14-15 22.23631178057861 25.344873962496155 26.918474826655736 25.5003394302695 16-17 21.55190080950917 28.62640682105407 27.468394558868738 22.353297810568023 18-19 21.545816682037096 27.075488011066707 29.720002049390303 21.65869325750589 20-21 22.26032807323155 26.321750008539123 29.579586791679475 21.83833512654985 22-23 22.059178279878402 26.702861888171604 29.236313915360178 22.001645916589815 24-25 22.010505260101787 26.91281765549749 29.339317126071663 21.737359958329066 26-27 21.74915462649862 27.173154267855317 29.403627420842298 21.67406368480377 28-29 21.569886344229257 26.598898025754004 29.6145438398743 22.216671790142435 30-31 22.254030467602554 26.746624910339172 29.47300782183967 21.5263368002186 32-33 22.024167862144346 26.913831676742834 29.351058424701986 21.710942036410835 34-35 22.13549672097551 27.101692454828026 29.364507548587625 21.39830327560884 36-37 22.231561891587255 26.867453547153055 29.205092734911364 21.69589182634833 38-39 21.653089455886875 26.709266232879052 29.534489531031184 22.10315478020289 40-41 21.549605919322335 26.535495013150257 29.870717628172283 22.044181439355125 42-43 22.076600314026702 26.426435882300826 29.50906375852179 21.98790004515068 44-45 21.630514140793114 26.969816323393793 29.307242032995184 22.092427502817912 46-47 22.08043396812486 26.64080320822165 28.729680426709702 22.549082396943778 48-49 21.92569821370436 26.83526266071057 28.56076980962903 22.678269315956044 50-51 21.671144385346388 27.043819734197495 28.654997913244333 22.630037967211784 52-53 21.765699183659528 27.058783345288113 28.85712333913994 22.318394131912424 54-55 21.64262902619804 27.078103118488915 28.50061481709191 22.77865303822113 56-57 22.00815700037572 27.363256566588106 27.870747515114253 22.75783891792192 58-59 21.45700976876046 27.421642842504358 28.62640682105407 22.494940567681116 60-61 21.904952002079277 27.846052279761143 27.927280761818558 22.321714956341022 62-63 21.385067629880112 28.267336561123063 27.788131468388155 22.559464340608667 64-65 21.369376985346857 27.961529101342354 27.93084161628582 22.738252297024967 66-67 21.35027069030297 28.046119821019914 27.754135071899444 22.849474416777678 68-69 21.457353749593043 28.047628451114658 27.6620749651753 22.832942834117002 70-71 21.536530382211293 27.827197971103594 27.46065597568057 23.175615671004543 72-73 21.6128488233084 27.722220087440654 27.428100556751033 23.236830532499912 74-75 21.529295105047378 27.822958105934383 27.253237666553165 23.394509122465077 76-77 21.63243544420535 27.565900707039653 27.336785275130648 23.464878573624347 78-79 21.621654797281142 27.658069901287703 27.140652218465007 23.57962308296615 80-81 21.618239146770502 27.639497301636094 27.142893739112612 23.599369812480788 82-83 21.677425965775182 27.433224032516996 27.14001178399426 23.749338217713564 84-85 21.70791155808717 27.3359993211391 27.05394115578203 23.9021479649917 86-87 21.766344331579425 27.28124701463343 27.064993064609872 23.88741558917727 88-89 21.760111692129357 27.128143876199353 27.0497971417318 24.06194728993949 90-91 21.864609584559503 27.002031251167463 27.001924511747205 24.13143465252583 92-93 21.84730120914028 26.831749325409028 27.022118471837963 24.298830993612732 94-95 21.752250059773885 26.76722555248147 27.131952983570724 24.348571404173924 96-97 21.801937100795847 26.67692429210643 27.107669843221643 24.413468763876082 98-99 21.793680520604784 26.722037587255365 26.94678403366233 24.53749785847752 100-101 21.838676811919836 26.752866094506267 26.82753051821593 24.580926575357964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 132.0 2 111.0 3 142.5 4 120.0 5 124.5 6 186.5 7 386.5 8 704.0 9 933.5 10 845.5 11 694.0 12 738.5 13 850.0 14 928.5 15 999.0 16 1078.0 17 1145.5 18 1300.5 19 1422.0 20 1565.5 21 1855.5 22 2165.0 23 2655.0 24 3294.0 25 3941.0 26 4943.0 27 6261.5 28 7597.0 29 9275.0 30 11169.0 31 13029.0 32 14881.5 33 16686.5 34 18441.5 35 20371.0 36 22612.0 37 24379.0 38 25837.5 39 27552.5 40 29248.0 41 30504.0 42 31348.5 43 32775.5 44 34480.0 45 35839.0 46 36742.0 47 37538.0 48 38433.5 49 38640.0 50 38011.0 51 36863.5 52 35660.5 53 34467.0 54 32424.5 55 29288.0 56 25204.5 57 21072.0 58 18443.5 59 16020.5 60 12745.5 61 9929.0 62 8002.0 63 6139.5 64 4336.5 65 3035.0 66 2149.0 67 1616.5 68 1216.0 69 869.0 70 627.5 71 465.5 72 367.0 73 284.5 74 220.5 75 164.5 76 104.5 77 53.5 78 33.5 79 21.0 80 13.0 81 8.5 82 3.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14046862725005976 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 1.0673907845749223E-4 44-45 0.0 46-47 0.002241520647607337 48-49 9.6065170611743E-4 50-51 3.202172353724767E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 5.336953922874612E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.003095433275267275 70-71 0.0 72-73 0.0 74-75 1.0673907845749223E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 5.336953922874612E-5 86-87 2.6684769614373057E-4 88-89 3.202172353724767E-4 90-91 3.202172353724767E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0032555418929535126 100-101 5.870649315162073E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 936864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.75989770894925 #Duplication Level Percentage of deduplicated Percentage of total 1 69.99065646877128 39.726625017496644 2 15.887536349478223 18.03549876087194 3 6.276434292211869 10.687493052086609 4 3.0079749025660196 6.829293911229353 5 1.6859047605751045 4.784589087863676 6 0.9825146943257698 3.346046012848214 7 0.5970753128345813 2.3722953576720793 8 0.3907747709611846 1.7744268821595928 9 0.2710427503643362 1.384592290688879 >10 0.887224354524125 7.641955445143384 >50 0.014738761223005421 0.5684049610647434 >100 0.0068003014329492744 0.7282239035296674 >500 0.0 0.0 >1k 0.0011333834842378989 1.4404722766980464 >5k 1.8889724737298315E-4 0.6800830406472429 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 6343 0.6770459746558732 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3922 0.4186306657102845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.36472743108925093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1910 0.20387163985381018 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1856 0.1981077296171056 RNA PCR Primer, Index 23 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 1261 0.1345979779348977 RNA PCR Primer, Index 23 (96% over 25bp) TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 1069 0.11410407487105918 RNA PCR Primer, Index 23 (96% over 30bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0673907845749223E-4 0.0 12-13 0.0 0.0 0.0 2.1347815691498447E-4 0.0 14-15 0.0 0.0 0.0 2.6684769614373057E-4 0.0 16-17 0.0 0.0 0.0 3.735867746012228E-4 0.0 18-19 0.0 0.0 0.0 7.471735492024456E-4 0.0 20-21 0.0 0.0 0.0 9.6065170611743E-4 0.0 22-23 0.0 0.0 0.0 0.001814564333777368 0.0 24-25 0.0 0.0 0.0 0.0036291286675547356 0.0 26-27 0.0 0.0 0.0 0.007044779178194487 0.0 28-29 0.0 0.0 0.0 0.017398469788571232 0.0 30-31 0.0 0.0 0.0 0.04456356525600301 0.0 32-33 0.0 0.0 0.0 0.1017223417699901 0.0 34-35 0.0 0.0 0.0 0.18604621375140895 0.0 36-37 0.0 1.0673907845749223E-4 0.0 0.3320652730812583 0.0 38-39 0.0 1.0673907845749223E-4 0.0 0.5237686579909144 0.0 40-41 0.0 1.0673907845749223E-4 0.0 0.7138175871844792 0.0 42-43 0.0 1.0673907845749223E-4 0.0 0.8846001127164669 0.0 44-45 0.0 1.0673907845749223E-4 0.0 1.0888453393448783 0.0 46-47 0.0 1.0673907845749223E-4 0.0 1.343418041465997 0.0 48-49 0.0 1.0673907845749223E-4 0.0 1.611920193325819 0.0 50-51 0.0 1.0673907845749223E-4 0.0 1.8683074597807152 0.0 52-53 0.0 1.0673907845749223E-4 0.0 2.117009512586672 0.0 54-55 0.0 1.0673907845749223E-4 0.0 2.378947211121358 0.0 56-57 0.0 1.0673907845749223E-4 0.0 2.682139563479865 0.0 58-59 0.0 1.0673907845749223E-4 0.0 2.995365389213376 0.0 60-61 0.0 1.0673907845749223E-4 0.0 3.3190516446357208 0.0 62-63 0.0 1.0673907845749223E-4 0.0 3.651543874030809 0.0 64-65 0.0 1.0673907845749223E-4 0.0 3.9968447928407964 0.0 66-67 0.0 1.0673907845749223E-4 0.0 4.3953551422618435 0.0 68-69 0.0 1.0673907845749223E-4 0.0 4.857108395668956 0.0 70-71 0.0 1.0673907845749223E-4 0.0 5.309148392936435 0.0 72-73 0.0 1.0673907845749223E-4 0.0 5.755691327663353 0.0 74-75 0.0 1.0673907845749223E-4 0.0 6.1996191549680635 0.0 76-77 0.0 1.0673907845749223E-4 0.0 6.669324469720259 0.0 78-79 0.0 1.0673907845749223E-4 0.0 7.183379871571541 0.0 80-81 0.0 1.0673907845749223E-4 0.0 7.726521672302489 0.0 82-83 0.0 1.0673907845749223E-4 0.0 8.269770212111897 0.0 84-85 0.0 1.0673907845749223E-4 0.0 8.834313198073573 0.0 86-87 0.0 1.0673907845749223E-4 0.0 9.445607900399631 0.0 88-89 0.0 1.0673907845749223E-4 0.0 10.116089421730369 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1750 0.0 62.520634 1 GTATCAA 3145 0.0 42.502968 1 TCAACGC 3825 0.0 34.6463 4 ATCAACG 3895 0.0 34.023643 3 CAACGCA 3920 0.0 33.806656 5 AACGCAG 3955 0.0 33.74768 6 TATCAAC 3980 0.0 33.416355 2 ACGCAGA 4380 0.0 30.364632 7 CGCAGAG 4550 0.0 29.021343 8 GCAGAGT 5060 0.0 25.626907 9 GAGTACT 3270 0.0 24.693647 12-13 CCGTCCG 100 0.0016536292 23.74948 9 GTACATG 2900 0.0 23.620987 1 CAGAGTA 4900 0.0 23.410202 10-11 TACATGG 2965 0.0 22.267641 2 GTACTTT 3655 0.0 22.092539 14-15 GTATGGA 325 1.8189894E-12 21.955406 1 AGAGTAC 4620 0.0 21.89887 10-11 ACATGGG 3110 0.0 21.229437 3 AGTACTT 3535 0.0 19.88641 12-13 >>END_MODULE