FastQCFastQC Report
Fri 10 Feb 2017
SRR2031501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049575
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.17397518043017413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.11976276111759522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.050.506331
GTATCAA20200.041.150991
TCAACGC22150.036.4559824
CAACGCA22550.035.8093155
ATCAACG22450.035.757243
AACGCAG23300.034.656656
TATCAAC23800.033.9285742
GTATCGC753.0782673E-431.6666683
ACGCAGA26100.030.9386987
CGCAGAG26750.030.0093468
GTACATG26700.026.6853921
TACATGG26600.026.0714282
ACATGGG26150.025.0669213
GCAGAGT32500.024.5538469
GCACCGT1302.9412733E-421.9230776
GTATTAT6100.021.024591
CAGAGTA31550.021.00237810-11
GTATATA9750.020.9487171
GACCGTT1456.1455515E-419.6551728
AGAGTAC29250.019.56837710-11
Produced by FastQC (version 0.11.5)