##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031501_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1049575 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21109401424386 34.0 31.0 34.0 30.0 34.0 2 32.3629316628159 34.0 31.0 34.0 30.0 34.0 3 32.47373555963128 34.0 31.0 34.0 30.0 34.0 4 35.69464306981397 37.0 35.0 37.0 35.0 37.0 5 35.685061096157966 37.0 35.0 37.0 35.0 37.0 6 35.80652740394922 37.0 37.0 37.0 35.0 37.0 7 35.83202153252507 37.0 37.0 37.0 35.0 37.0 8 35.88062977871996 37.0 37.0 37.0 35.0 37.0 9 37.70654407736465 39.0 39.0 39.0 35.0 39.0 10-11 37.7063177952981 39.0 39.0 39.0 35.0 39.0 12-13 37.675355262844484 39.0 39.0 39.0 35.0 39.0 14-15 39.19729795393374 41.0 39.5 41.0 36.0 41.0 16-17 39.208857394659745 41.0 40.0 41.0 36.0 41.0 18-19 39.16002858299788 41.0 39.5 41.0 36.0 41.0 20-21 39.12279351165948 41.0 39.5 41.0 36.0 41.0 22-23 39.05610794845533 41.0 39.0 41.0 36.0 41.0 24-25 39.00438129719172 41.0 39.0 41.0 36.0 41.0 26-27 38.906395922158964 41.0 39.0 41.0 35.0 41.0 28-29 38.81289045566062 41.0 39.0 41.0 35.0 41.0 30-31 38.65774623061715 40.5 39.0 41.0 35.0 41.0 32-33 38.556395207584025 40.0 38.0 41.0 34.5 41.0 34-35 38.435990758164024 40.0 38.0 41.0 34.0 41.0 36-37 38.30731534192411 40.0 38.0 41.0 34.0 41.0 38-39 38.16440273444013 40.0 38.0 41.0 33.5 41.0 40-41 38.04380058595146 40.0 38.0 41.0 33.0 41.0 42-43 37.90514493961842 40.0 38.0 41.0 33.0 41.0 44-45 38.0853869423338 40.0 38.0 41.0 33.0 41.0 46-47 37.98326036729152 40.0 38.0 41.0 33.0 41.0 48-49 37.8808827382512 40.0 38.0 41.0 33.0 41.0 50-51 36.85012505061573 39.0 36.5 40.0 31.5 40.5 52-53 36.92437605697545 39.5 37.5 40.0 31.5 40.5 54-55 37.72539170616678 40.0 38.0 41.0 33.0 41.0 56-57 37.78163018364576 40.0 38.0 41.0 33.0 41.0 58-59 37.607817449920205 40.0 37.0 41.0 32.0 41.0 60-61 37.46020627396803 40.0 37.0 41.0 32.0 41.0 62-63 37.22557606650311 40.0 36.5 41.0 31.5 41.0 64-65 36.97161279565539 39.5 36.0 41.0 31.0 41.0 66-67 36.70731296000762 39.0 35.0 41.0 31.0 41.0 68-69 36.42069313769859 39.0 35.0 41.0 31.0 41.0 70-71 36.06883405187814 37.5 35.0 40.5 30.5 41.0 72-73 35.7070318938618 37.0 35.0 40.0 30.0 41.0 74-75 35.31865850463283 37.0 35.0 39.0 30.0 41.0 76-77 34.9354953195341 36.0 35.0 39.0 29.0 41.0 78-79 34.51795488650168 36.0 35.0 38.0 29.0 39.5 80-81 34.153496415215685 35.0 34.0 37.0 29.0 39.0 82-83 33.826480718386016 35.0 34.0 37.0 29.0 39.0 84-85 33.50704904366053 35.0 34.0 36.0 28.5 38.0 86-87 33.22144677607603 35.0 34.0 36.0 27.5 37.0 88-89 32.991969606745585 35.0 34.0 36.0 27.0 37.0 90-91 32.797395136126525 35.0 34.0 35.0 27.0 36.5 92-93 32.633769382845436 35.0 34.0 35.0 26.0 36.0 94-95 32.49642712526499 35.0 34.0 35.0 26.0 36.0 96-97 32.374385346449756 35.0 34.0 35.0 25.5 36.0 98-99 32.24560560226759 35.0 33.0 35.0 25.0 35.5 100-101 30.73284043541433 34.0 30.0 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3077834864454374 1101 2 0.19461392992437965 1101 3 0.3514967428509621 1101 4 0.13961977114396973 1101 5 0.20147558314192082 1101 6 0.20340756243339086 1101 7 0.22349061859652863 1101 8 0.20376810197567607 1101 9 0.33648039681843755 1101 10-11 0.544653022151671 1101 12-13 0.48095661850531 1101 14-15 0.6034666295687003 1101 16-17 0.45675947345094414 1101 18-19 0.6311303816127918 1101 20-21 0.5443385366379161 1101 22-23 0.660270663104825 1101 24-25 0.663464150300257 1101 26-27 0.6332104006351642 1101 28-29 0.696475982704591 1101 30-31 0.7582619269040407 1101 32-33 0.6959226824313802 1101 34-35 0.6876811254278152 1101 36-37 0.6642033002140977 1101 38-39 0.7376852259942339 1101 40-41 0.6626533481618893 1101 42-43 0.728638166267551 1101 44-45 0.6562033678810479 1101 46-47 0.6825868130717581 1101 48-49 0.7698153167638111 1101 50-51 0.5906750867995214 1101 52-53 0.7518745876790973 1101 54-55 0.8002281760994379 1101 56-57 0.6866180504490842 1101 58-59 0.6111250009928568 1101 60-61 0.5599720043623293 1101 62-63 0.8118598718229464 1101 64-65 0.7046590987275749 1101 66-67 0.5996297936809825 1101 68-69 0.5255604877846949 1101 70-71 0.7053928230773963 1101 72-73 0.6292269879313395 1101 74-75 0.5844102893462662 1101 76-77 0.790997027280163 1101 78-79 0.7643913067990837 1101 80-81 0.596660414199647 1101 82-83 0.6732814449967037 1101 84-85 0.6960031518378287 1101 86-87 0.745170669404267 1101 88-89 0.7440701206109495 1101 90-91 0.6050992132926254 1101 92-93 0.5531738165560824 1101 94-95 0.6617731480970406 1101 96-97 0.7674975951246807 1101 98-99 0.6957437914682032 1101 100-101 0.47216746538326504 1102 1 0.13456771123951228 1102 2 0.2604145502976465 1102 3 0.3539534731189278 1102 4 0.028405481705952695 1102 5 0.18826984338590336 1102 6 0.19070359517241542 1102 7 0.28873081774155906 1102 8 0.2533458310967234 1102 9 0.37694150619181244 1102 10-11 0.5079291403875175 1102 12-13 0.6381026408178982 1102 14-15 0.7153928494623472 1102 16-17 0.7355722927607147 1102 18-19 0.6272286117243553 1102 20-21 0.7434949723589099 1102 22-23 0.7375322234217876 1102 24-25 0.6755294980220796 1102 26-27 0.6759115657899883 1102 28-29 0.7245833880846391 1102 30-31 0.7260442228064505 1102 32-33 0.7412548949222568 1102 34-35 0.5717211759960534 1102 36-37 0.5653247943231534 1102 38-39 0.5489878143583269 1102 40-41 0.6417108630195614 1102 42-43 0.7119200190461967 1102 44-45 0.8100878689758204 1102 46-47 0.8037257714702548 1102 48-49 0.8430317436223262 1102 50-51 0.8417144726821206 1102 52-53 0.8935194239839674 1102 54-55 0.6738222356344181 1102 56-57 0.5807864114124897 1102 58-59 0.8175245552624943 1102 60-61 0.5261707844686825 1102 62-63 0.6549207492079887 1102 64-65 0.6103323861845666 1102 66-67 0.8769861694499284 1102 68-69 0.7871924865335203 1102 70-71 0.6115222673480574 1102 72-73 0.423254266108799 1102 74-75 0.5391579042977881 1102 76-77 0.5347265002235488 1102 78-79 0.39014396554165387 1102 80-81 0.41117727896870093 1102 82-83 0.4520274699158975 1102 84-85 0.33968766075243906 1102 86-87 0.44165532950227515 1102 88-89 0.595115371366866 1102 90-91 0.4160026644447541 1102 92-93 0.42303553587628073 1102 94-95 0.40556778629952817 1102 96-97 0.40777396098851426 1102 98-99 0.618723342609897 1102 100-101 0.27912190183410246 1103 1 0.3335135050994751 1103 2 0.22999532132438816 1103 3 0.36363681555388894 1103 4 0.25309701560754405 1103 5 0.2637737966589313 1103 6 0.1986259701437305 1103 7 0.20765112703867317 1103 8 0.3117941219689797 1103 9 0.34135017727474803 1103 10-11 0.31854005759434756 1103 12-13 0.38929772435502485 1103 14-15 0.41557725639225396 1103 16-17 0.3519366270358688 1103 18-19 0.3807604397273039 1103 20-21 0.3659376123063467 1103 22-23 0.35345730513859763 1103 24-25 0.37430710959618807 1103 26-27 0.4219108060017831 1103 28-29 0.3827301380114321 1103 30-31 0.5704301002431151 1103 32-33 0.5893031889602938 1103 34-35 0.5688699915970403 1103 36-37 0.5485975820335867 1103 38-39 0.6667826873708123 1103 40-41 0.5822653501229595 1103 42-43 0.5825522261517975 1103 44-45 0.7057337285258072 1103 46-47 0.4969058808481037 1103 48-49 0.3524954597781118 1103 50-51 0.3863460941176413 1103 52-53 0.42376453482598464 1103 54-55 0.5719710433209428 1103 56-57 0.484202292568952 1103 58-59 0.6283926924359235 1103 60-61 0.6142152004210644 1103 62-63 0.61156137729958 1103 64-65 0.6514143720809926 1103 66-67 0.5583816471271987 1103 68-69 0.6560221752577817 1103 70-71 0.6253448726301727 1103 72-73 0.6626943971722667 1103 74-75 0.588573817112092 1103 76-77 0.6798104330772716 1103 78-79 0.5882765485841333 1103 80-81 0.6550574223787251 1103 82-83 0.6708009659705425 1103 84-85 0.665571508129986 1103 86-87 0.5752059147766317 1103 88-89 0.5198330229881449 1103 90-91 0.41528838438365057 1103 92-93 0.328020458829009 1103 94-95 0.5274663070868542 1103 96-97 0.4034605800101261 1103 98-99 0.48257879290313355 1103 100-101 0.46859210784751326 1104 1 0.14959415162566359 1104 2 0.1811779395862132 1104 3 0.3019566090717021 1104 4 0.1621369580633285 1104 5 0.12816408184129102 1104 6 0.013945002046916954 1104 7 -0.014211443833794135 1104 8 -0.030995926266612628 1104 9 0.2554463945887875 1104 10-11 0.3134308928317111 1104 12-13 0.30502295072381713 1104 14-15 0.3420338443587383 1104 16-17 0.4441131523807087 1104 18-19 0.3577480423337178 1104 20-21 0.38546985399205624 1104 22-23 0.36944592323117575 1104 24-25 0.4244150050011086 1104 26-27 0.4669095366440814 1104 28-29 0.520873279248633 1104 30-31 0.3248728522106177 1104 32-33 0.3923993639624115 1104 34-35 0.4562673851825565 1104 36-37 0.5226550839376145 1104 38-39 0.3995035556114743 1104 40-41 0.44155175953312664 1104 42-43 0.23440971959345092 1104 44-45 0.21014474655068938 1104 46-47 0.46427809883913085 1104 48-49 0.44956959026808363 1104 50-51 0.351602715933673 1104 52-53 0.49098760328667623 1104 54-55 0.5783601437694514 1104 56-57 0.47281509615700656 1104 58-59 0.44676347182155496 1104 60-61 0.5074357724782672 1104 62-63 0.44737524714810206 1104 64-65 0.4703828073860592 1104 66-67 0.33165320505221985 1104 68-69 0.43354079250412525 1104 70-71 0.39723600405100257 1104 72-73 0.37535412468347573 1104 74-75 0.2929932975216758 1104 76-77 0.15043009119692385 1104 78-79 0.17830574381135023 1104 80-81 0.3374747025682865 1104 82-83 0.2290750626109741 1104 84-85 0.24534006190843627 1104 86-87 0.2752884230274546 1104 88-89 0.3620095483329919 1104 90-91 0.16790114565977632 1104 92-93 0.33521348582401345 1104 94-95 0.27919580695991897 1104 96-97 0.22291031344500567 1104 98-99 0.21666422067667668 1104 100-101 0.1746247303405326 1105 1 0.22540465905458262 1105 2 0.15568385708592558 1105 3 0.10926111945273931 1105 4 0.2312256454302002 1105 5 0.11826917380781055 1105 6 0.19361006007288495 1105 7 0.18327350830472255 1105 8 0.09610657596515182 1105 9 0.12607787444984098 1105 10-11 -0.013236967057444815 1105 12-13 0.14039470582152802 1105 14-15 0.1960160034727494 1105 16-17 0.15656641783294134 1105 18-19 0.014208789732926164 1105 20-21 0.06643806566088983 1105 22-23 0.13843528587358378 1105 24-25 0.16601714926613198 1105 26-27 0.06224138507830901 1105 28-29 0.05709586827628499 1105 30-31 -0.13209821877286743 1105 32-33 -0.03312642732087312 1105 34-35 0.10971911270356571 1105 36-37 0.10634141225941818 1105 38-39 0.09506222680354881 1105 40-41 -0.2213678570447115 1105 42-43 -0.09516275320549283 1105 44-45 0.11304405624937175 1105 46-47 -0.03177377098471368 1105 48-49 -0.09060996762088536 1105 50-51 -0.05080770399226253 1105 52-53 -0.07809666473760757 1105 54-55 -0.13635361541173552 1105 56-57 -0.16843427408667822 1105 58-59 -0.1293072244016784 1105 60-61 -0.2548660200070785 1105 62-63 -0.12622393173804625 1105 64-65 -0.06381185581584248 1105 66-67 -0.06181191307680223 1105 68-69 -0.13357410598357689 1105 70-71 -0.05361924922151928 1105 72-73 0.14346238089928676 1105 74-75 0.038285592839820026 1105 76-77 -0.024084775471514774 1105 78-79 0.05767426395028963 1105 80-81 0.03875918448875382 1105 82-83 0.10913962920604092 1105 84-85 0.2456770367800516 1105 86-87 0.10797542750866995 1105 88-89 0.021176590643776194 1105 90-91 0.007683404650933312 1105 92-93 -0.07329728411385616 1105 94-95 -0.07953162124785962 1105 96-97 0.11571639711200277 1105 98-99 -0.10675707967068604 1105 100-101 -0.18269292758467515 1106 1 -0.003404669476601896 1106 2 0.08536432678477013 1106 3 0.07694100601349874 1106 4 0.05477708219432742 1106 5 0.047799663748676835 1106 6 0.13528261755288895 1106 7 0.14604496642261466 1106 8 0.1366545999031814 1106 9 0.13966520147493355 1106 10-11 0.06896347721825435 1106 12-13 0.10713066383558356 1106 14-15 0.16216200614512388 1106 16-17 0.1828789504771393 1106 18-19 0.1604332853080095 1106 20-21 0.28432194566869384 1106 22-23 0.09154353769694268 1106 24-25 0.17341597585806312 1106 26-27 0.14695582571673782 1106 28-29 0.10321516825887755 1106 30-31 0.20140065165930565 1106 32-33 0.20682807532317327 1106 34-35 0.3356232389298839 1106 36-37 0.15787833769535808 1106 38-39 0.300136996432542 1106 40-41 0.1893380756510865 1106 42-43 0.21241374171512462 1106 44-45 0.1547950646797105 1106 46-47 -0.008636509090692357 1106 48-49 0.26049736544535307 1106 50-51 0.07714600575181407 1106 52-53 -0.01649536337479418 1106 54-55 0.07638375639753292 1106 56-57 0.17112671523433676 1106 58-59 0.33394144203704457 1106 60-61 0.24015213488530662 1106 62-63 0.31952010420675236 1106 64-65 0.3379215116387613 1106 66-67 0.30700037309716066 1106 68-69 0.16142454105217752 1106 70-71 0.3038527842732677 1106 72-73 0.20107663296176526 1106 74-75 0.2242856641588702 1106 76-77 0.317403401299039 1106 78-79 0.22157172394345537 1106 80-81 0.33119663873443983 1106 82-83 0.36486717786556255 1106 84-85 0.23236135141451086 1106 86-87 0.21029518435267391 1106 88-89 0.04933293038253339 1106 90-91 0.2711522162029425 1106 92-93 0.19134462085981596 1106 94-95 0.15024628285319608 1106 96-97 0.05231796067720751 1106 98-99 0.14120718109691666 1106 100-101 0.2885974856672391 1107 1 0.3172119711924779 1107 2 0.36630519870288936 1107 3 0.3980271650796965 1107 4 0.369697029795681 1107 5 0.2658416646584314 1107 6 0.3014677268891788 1107 7 0.25480338798180924 1107 8 0.3260637876265946 1107 9 0.39179853298615797 1107 10-11 0.34108013643379564 1107 12-13 0.382683299678682 1107 14-15 0.41039911586405253 1107 16-17 0.3653947100915289 1107 18-19 0.4897809182910393 1107 20-21 0.49022674959351775 1107 22-23 0.5104813328541624 1107 24-25 0.5573224274898649 1107 26-27 0.5138419914206125 1107 28-29 0.5884570629657233 1107 30-31 0.48789326138117417 1107 32-33 0.4641558811592503 1107 34-35 0.553257217615112 1107 36-37 0.5789977827851303 1107 38-39 0.5565975110090875 1107 40-41 0.6133839576526228 1107 42-43 0.4062794496922564 1107 44-45 0.4712572972445699 1107 46-47 0.565235524207047 1107 48-49 0.37108456604196505 1107 50-51 0.34388809470846127 1107 52-53 0.3084186884030302 1107 54-55 0.5076437251223354 1107 56-57 0.4599591079039982 1107 58-59 0.42094095592444347 1107 60-61 0.49911446994378394 1107 62-63 0.5211728870549805 1107 64-65 0.6540015894167794 1107 66-67 0.5598496957607395 1107 68-69 0.6006614418793461 1107 70-71 0.7535918647981035 1107 72-73 0.6616048753230643 1107 74-75 0.7477996530739333 1107 76-77 0.6570321205973144 1107 78-79 0.39060615594333115 1107 80-81 0.4067607929082726 1107 82-83 0.4999562106576647 1107 84-85 0.8011804491973322 1107 86-87 0.4795312847534774 1107 88-89 0.48923164716239853 1107 90-91 0.4908746244321023 1107 92-93 0.5091685543892837 1107 94-95 0.5186596035098603 1107 96-97 0.44927185026131156 1107 98-99 0.36312685733421546 1107 100-101 0.5975283893486178 1108 1 0.15464130753488803 1108 2 0.0676721924597814 1108 3 0.15610485546132935 1108 4 0.13749062738251894 1108 5 0.1098911670166558 1108 6 0.1446921284836975 1108 7 0.21501048366399544 1108 8 0.27131766052973205 1108 9 0.32599605565568623 1108 10-11 0.2266713879687643 1108 12-13 0.28546061650630605 1108 14-15 0.21616809133782056 1108 16-17 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-0.8725017906708317 2316 52-53 -0.7778492265501527 2316 54-55 -0.7526142152049076 2316 56-57 -0.7062979574560515 2316 58-59 -0.8215415009834288 2316 60-61 -0.7890339244914273 2316 62-63 -0.7821099898371386 2316 64-65 -1.0161175811143295 2316 66-67 -0.7812279138289 2316 68-69 -0.5556825952192597 2316 70-71 -0.9262810899660892 2316 72-73 -0.7279434666141142 2316 74-75 -0.5488303233019565 2316 76-77 -0.6864925972257652 2316 78-79 -0.5764891279835211 2316 80-81 -0.5528741707805906 2316 82-83 -0.6554680609321508 2316 84-85 -0.6626001090317288 2316 86-87 -0.5158327890937571 2316 88-89 -0.6192460849226435 2316 90-91 -0.46926047050183683 2316 92-93 -0.4738805312700052 2316 94-95 -0.4455069902428761 2316 96-97 -0.5249890649543687 2316 98-99 -0.5222576907452314 2316 100-101 -0.3909234437076421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 21.0 7 296.0 8 552.0 9 495.0 10 595.0 11 776.0 12 1125.0 13 1470.0 14 1572.0 15 1728.0 16 1977.0 17 2322.0 18 2830.0 19 3556.0 20 4650.0 21 5523.0 22 6286.0 23 6463.0 24 6219.0 25 6082.0 26 6129.0 27 6951.0 28 7823.0 29 9313.0 30 11006.0 31 13761.0 32 17160.0 33 23373.0 34 33274.0 35 52218.0 36 96420.0 37 211417.0 38 361871.0 39 144065.0 40 256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43393754614963 20.14596384250768 13.922111330776744 23.497987280565944 2 14.0904651882905 21.447061906009576 41.9369744896744 22.52549841602553 3 17.20305838077317 27.67396327084772 31.17557106447848 23.947407283900628 4 11.36631493699831 18.567705976228474 41.9409761093776 28.125002977395614 5 12.169116070790558 38.98968630159826 36.667031893861804 12.174165733749374 6 27.669771097825308 40.33575494843151 19.895481504418456 12.098992449324726 7 24.968391968177595 33.83188433413524 24.576137960603102 16.623585737084056 8 24.06955196150823 36.66960436367101 21.971845747088107 17.288997927732655 9 25.529857323202247 16.777362265678967 21.401519662720624 36.291260748398166 10-11 22.902555796393777 27.229307100493056 30.886358764261722 18.98177833885144 12-13 24.33880380153872 24.929376176071266 30.06450229854941 20.667317723840604 14-15 21.55839268275254 26.395731605649907 27.631993902293782 24.413881809303767 16-17 21.158992925708027 29.39680346807041 28.095610127908916 21.34859347831265 18-19 21.086820856060786 28.86454040921325 29.595455303337065 20.453183431388897 20-21 21.98399352118715 28.00585951456542 29.49336636257533 20.516780601672107 22-23 21.585165424100232 28.011861944120238 29.13079103446633 21.272181597313196 24-25 21.355453397803874 28.098944810994926 29.31624705237834 21.229354738822856 26-27 21.367505895243312 28.28173308243813 29.105256889693447 21.245504132625108 28-29 21.406950432317842 28.307791248838814 29.10025486506443 21.18500345377891 30-31 21.774289593406856 28.096134149536717 29.06948050401353 21.060095753042898 32-33 21.487697401329108 28.227758854774553 29.043136507634042 21.241407236262297 34-35 21.604506585999093 28.321892194459664 29.002072267346307 21.07152895219494 36-37 21.727699306862302 28.11566586475478 28.94595431484172 21.210680513541195 38-39 21.49384274587333 28.40025724698092 28.981016125574637 21.12488388157111 40-41 21.701164757163614 28.219850892027726 28.93709358549889 21.141890765309768 42-43 21.567062858776172 28.16649596265155 28.872067265321675 21.394373913250604 44-45 21.70297501369602 28.380153871805252 28.716242288545363 21.200628825953363 46-47 21.64223614320082 28.251625658004432 28.56851582783508 21.537622370959674 48-49 21.488265914485975 28.425960904960647 28.591361895175215 21.494411285378163 50-51 21.564633303956363 28.47533525474597 28.518352666555515 21.441678774742158 52-53 21.6966867541624 28.39673201057571 28.424552795179004 21.48202844008289 54-55 21.537003072672274 28.333563585260702 28.56918276445228 21.56025057761475 56-57 21.59092966200605 28.37034037586642 28.474144296500963 21.564585665626563 58-59 21.576638163066004 28.36714860776981 28.488149965462213 21.568063263701976 60-61 21.629707262463377 28.34180501631613 28.48648262391921 21.54200509730129 62-63 21.558154491103544 28.32384536598147 28.53259652716576 21.585403615749232 64-65 21.51718552747541 28.259247790772456 28.50358478431746 21.719981897434675 66-67 21.43886811328395 28.258437939165855 28.437796250863446 21.864897696686754 68-69 21.484505633232498 28.325607984184074 28.374056165590833 21.815830216992595 70-71 21.53971845747088 27.98165924302694 28.53712216849677 21.941500131005405 72-73 21.513803206059595 28.022199461686874 28.47252459328776 21.991472738965772 74-75 21.435581068527735 28.156634828382916 28.337041183336115 22.070742919753233 76-77 21.422766357811497 28.052592716099372 28.37801014696425 22.146630779124884 78-79 21.459590786747015 28.03934926041493 28.358621346735585 22.14243860610247 80-81 21.414048543458065 28.088988400066693 28.389109877807684 22.107853178667554 82-83 21.41828835481028 28.05087773622657 28.3640521163328 22.16678179263035 84-85 21.528618726627442 27.974465855227116 28.28421027558774 22.212705142557702 86-87 21.44220279636996 27.89619607936546 28.413786532644163 22.24781459162042 88-89 21.528571088297642 27.740799847557344 28.43041230974442 22.30021675440059 90-91 21.55986947097635 27.791534668794508 28.440940380630252 22.207655479598884 92-93 21.576209418097804 27.727889860181502 28.487054283876805 22.208846437843892 94-95 21.49046042445752 27.649334254341042 28.427125264988213 22.433080056213228 96-97 21.43377081199533 27.5750660981826 28.593144844341754 22.398018245480316 98-99 21.44911035419098 27.474406307314865 28.449277088345283 22.62720625014887 100-101 21.722840197222684 27.39804206464521 28.455803539527903 22.423314198604196 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 12.0 2 14.5 3 31.5 4 33.0 5 33.5 6 55.0 7 112.5 8 208.5 9 273.0 10 279.0 11 304.5 12 365.0 13 425.0 14 473.5 15 512.0 16 567.0 17 655.0 18 776.5 19 866.5 20 962.5 21 1173.0 22 1590.5 23 2162.0 24 2956.5 25 4042.0 26 5562.0 27 7663.5 28 10228.0 29 13408.5 30 16848.5 31 20136.0 32 23663.5 33 27325.0 34 30464.0 35 32753.5 36 34723.5 37 36388.0 38 37179.5 39 37946.5 40 38764.5 41 39225.0 42 39449.5 43 40106.0 44 40895.0 45 41366.0 46 41460.5 47 40777.0 48 40368.0 49 39401.5 50 37354.0 51 34832.5 52 32302.5 53 30080.5 54 27249.0 55 24005.5 56 20694.5 57 17713.5 58 15251.5 59 12805.0 60 10382.5 61 8127.0 62 6175.5 63 4540.5 64 3294.5 65 2431.5 66 1814.5 67 1393.5 68 1037.5 69 765.5 70 608.0 71 448.5 72 320.0 73 228.0 74 161.0 75 125.5 76 96.5 77 77.5 78 57.0 79 43.5 80 35.5 81 24.0 82 21.0 83 13.5 84 10.0 85 10.0 86 8.5 87 7.0 88 6.5 89 7.0 90 4.0 91 2.5 92 2.5 93 2.0 94 1.5 95 2.5 96 2.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 4.287449682014148E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1049575.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.27596632022129 #Duplication Level Percentage of deduplicated Percentage of total 1 77.10088506800173 52.64137432162139 2 14.035046586946711 19.16512736146221 3 4.62929391095607 9.482085454525265 4 1.8773162627978748 5.127023277247656 5 0.8753924708287443 2.988413342763932 6 0.4797011425872354 1.9651235433034657 7 0.2721301865515814 1.3005966016197916 8 0.18565198096036423 1.0140454719465755 9 0.12083901853932413 0.7425360683963542 >10 0.4075908197633897 4.3681167681099335 >50 0.013206234936677483 0.5937366941665502 >100 0.002525415477129686 0.24503522309381193 >500 1.403005510725642E-4 0.07146050152005207 >1k 2.806011021451284E-4 0.2953253702229497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1826 0.17397518043017413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.11976276111759522 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.527665960031442E-5 0.0 18-19 0.0 0.0 0.0 9.527665960031442E-5 0.0 20-21 0.0 0.0 0.0 9.527665960031442E-5 0.0 22-23 0.0 0.0 0.0 5.716599576018865E-4 0.0 24-25 0.0 0.0 0.0 8.574899364028297E-4 0.0 26-27 0.0 0.0 0.0 0.0011909582450039301 0.0 28-29 0.0 0.0 0.0 0.004716194650215564 0.0 30-31 0.0 0.0 0.0 0.01500607388704952 0.0 32-33 0.0 0.0 0.0 0.03982564371293142 0.0 34-35 0.0 0.0 0.0 0.07912726579806112 0.0 36-37 0.0 0.0 0.0 0.13953266798466046 0.0 38-39 0.0 0.0 0.0 0.24276492866160115 0.0 40-41 0.0 0.0 0.0 0.35142795893575973 0.0 42-43 0.0 0.0 0.0 0.4523735797822928 0.0 44-45 0.0 0.0 0.0 0.5747564490388968 0.0 46-47 0.0 0.0 0.0 0.7232927613557869 0.0 48-49 0.0 0.0 0.0 0.8914560655503418 0.0 50-51 0.0 0.0 0.0 1.0674796941619227 0.0 52-53 0.0 0.0 0.0 1.2295452921420575 0.0 54-55 0.0 0.0 0.0 1.399423576209418 0.0 56-57 0.0 0.0 0.0 1.6008384346044826 0.0 58-59 0.0 0.0 0.0 1.8162589619607936 0.0 60-61 0.0 0.0 0.0 2.0492103946835623 0.0 62-63 0.0 0.0 0.0 2.2890217468975536 0.0 64-65 0.0 0.0 0.0 2.527832694185742 0.0 66-67 0.0 0.0 0.0 2.799323535716838 0.0 68-69 0.0 0.0 0.0 3.1114022342376675 0.0 70-71 0.0 0.0 0.0 3.4371054950813424 0.0 72-73 0.0 0.0 0.0 3.764476097468023 0.0 74-75 0.0 0.0 0.0 4.076650072648453 0.0 76-77 0.0 0.0 0.0 4.404639973322535 0.0 78-79 0.0 0.0 0.0 4.7650239382607245 0.0 80-81 0.0 0.0 0.0 5.151847176238001 0.0 82-83 0.0 0.0 0.0 5.541671628992687 0.0 84-85 0.0 0.0 0.0 5.932591763332777 0.0 86-87 0.0 0.0 0.0 6.337327013314913 0.0 88-89 0.0 0.0 0.0 6.777648095657766 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1185 0.0 50.50633 1 GTATCAA 2020 0.0 41.15099 1 TCAACGC 2215 0.0 36.455982 4 CAACGCA 2255 0.0 35.809315 5 ATCAACG 2245 0.0 35.75724 3 AACGCAG 2330 0.0 34.65665 6 TATCAAC 2380 0.0 33.928574 2 GTATCGC 75 3.0782673E-4 31.666668 3 ACGCAGA 2610 0.0 30.938698 7 CGCAGAG 2675 0.0 30.009346 8 GTACATG 2670 0.0 26.685392 1 TACATGG 2660 0.0 26.071428 2 ACATGGG 2615 0.0 25.066921 3 GCAGAGT 3250 0.0 24.553846 9 GCACCGT 130 2.9412733E-4 21.923077 6 GTATTAT 610 0.0 21.02459 1 CAGAGTA 3155 0.0 21.002378 10-11 GTATATA 975 0.0 20.948717 1 GACCGTT 145 6.1455515E-4 19.655172 8 AGAGTAC 2925 0.0 19.568377 10-11 >>END_MODULE