FastQCFastQC Report
Fri 10 Feb 2017
SRR2031501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049575
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.23895386227758855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.17216492389776814No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT13350.12719434056641973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.050.534931
GTATCAA20250.043.2292561
TCAACGC24150.035.599384
CAACGCA24600.034.948175
ATCAACG24800.034.8578573
AACGCAG25650.033.517546
TATCAAC27150.032.1905942
ACGCAGA28900.029.9126267
CGCAGAG28900.029.748278
GCAGAGT35650.023.9824739
GTACATG21250.022.8363251
GTGTACG1252.231535E-422.8363251
AGAGTAC32450.021.95621910-11
ACATGGG20100.021.0317273
GAGTACT25950.020.4088512-13
TACATGG21900.020.1706492
AGTACTT28900.019.64043212-13
TAATACC4850.019.587064
TATACTG6900.019.2748015
CAGAGTA35000.019.27086810-11
Produced by FastQC (version 0.11.5)