FastQCFastQC Report
Fri 10 Feb 2017
SRR2031500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482599
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.22723609013630794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.1762445543265576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.052.8589741
GTATCAA36100.044.6052631
TCAACGC42850.036.8028034
TATCAAC44550.035.8249172
ATCAACG44050.035.8002243
CAACGCA44100.035.6519285
AACGCAG45450.034.6974686
ACGCAGA50850.030.919377
CGCAGAG52300.029.8804978
GTACATG43900.025.3189091
GCAGAGT61800.024.6723299
GTATTAC3300.024.4696981
TACATGG44150.023.669312
TCGCCGG2300.021.68478294-95
CAGAGTA56100.021.54857410-11
GAGTACT35650.021.45161412-13
ACATGGG45500.021.4010983
GTATAGA5000.020.91
AGAGTAC53800.020.83643210-11
GTACTTT38500.019.6785714-15
Produced by FastQC (version 0.11.5)