##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031500_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1482599 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19302387226755 34.0 31.0 34.0 30.0 34.0 2 32.35861213989757 34.0 31.0 34.0 30.0 34.0 3 32.45948230101329 34.0 31.0 34.0 30.0 34.0 4 35.67460520342992 37.0 35.0 37.0 35.0 37.0 5 35.652842744396835 37.0 35.0 37.0 35.0 37.0 6 35.77995735866543 37.0 37.0 37.0 35.0 37.0 7 35.80833859998557 37.0 37.0 37.0 35.0 37.0 8 35.86331300641643 37.0 37.0 37.0 35.0 37.0 9 37.6913649611257 39.0 39.0 39.0 35.0 39.0 10-11 37.69791798051935 39.0 39.0 39.0 35.0 39.0 12-13 37.67224718214433 39.0 39.0 39.0 35.0 39.0 14-15 39.19623917188667 41.0 40.0 41.0 36.0 41.0 16-17 39.20700236544069 41.0 40.0 41.0 36.0 41.0 18-19 39.16076262023649 41.0 40.0 41.0 36.0 41.0 20-21 39.12738070105268 41.0 40.0 41.0 36.0 41.0 22-23 39.05561213787409 41.0 39.0 41.0 36.0 41.0 24-25 39.00533927245331 41.0 39.0 41.0 36.0 41.0 26-27 38.90274443730233 41.0 39.0 41.0 35.0 41.0 28-29 38.799454876200514 41.0 39.0 41.0 35.0 41.0 30-31 38.63494882972402 40.5 39.0 41.0 35.0 41.0 32-33 38.5228156770644 40.0 38.0 41.0 34.5 41.0 34-35 38.39847187270462 40.0 38.0 41.0 34.0 41.0 36-37 38.259414379747994 40.0 38.0 41.0 34.0 41.0 38-39 38.114149881390716 40.0 38.0 41.0 33.5 41.0 40-41 37.97886751576117 40.0 38.0 41.0 33.0 41.0 42-43 37.82943230097956 40.0 38.0 41.0 33.0 41.0 44-45 37.99681404074871 40.0 38.0 41.0 33.0 41.0 46-47 37.867864135885696 40.0 38.0 41.0 33.0 41.0 48-49 37.74010504526173 40.0 38.0 41.0 33.0 41.0 50-51 36.68218344946948 39.0 36.5 40.0 31.0 40.5 52-53 36.73618557681477 39.5 37.0 40.0 31.0 40.5 54-55 37.49779272750082 40.0 37.0 41.0 32.0 41.0 56-57 37.52493695193373 40.0 37.0 41.0 32.0 41.0 58-59 37.32875747251954 40.0 37.0 41.0 32.0 41.0 60-61 37.15602836640251 40.0 36.0 41.0 31.5 41.0 62-63 36.89680115796652 39.5 36.0 41.0 31.0 41.0 64-65 36.6164930638696 39.0 35.0 41.0 31.0 41.0 66-67 36.31484069529252 39.0 35.0 41.0 31.0 41.0 68-69 36.00845811982876 37.5 35.0 40.5 30.0 41.0 70-71 35.634759972183986 37.0 35.0 40.0 30.0 41.0 72-73 35.26590871840599 37.0 35.0 39.0 29.0 41.0 74-75 34.87931699670646 36.0 35.0 39.0 29.0 41.0 76-77 34.504198707809735 36.0 35.0 38.5 29.0 40.5 78-79 34.101629975468754 35.0 34.0 37.0 28.5 39.0 80-81 33.75632082579308 35.0 34.0 37.0 28.0 39.0 82-83 33.44728379015499 35.0 34.0 36.5 27.0 39.0 84-85 33.14747682954056 35.0 34.0 36.0 27.0 37.0 86-87 32.87866510094773 35.0 34.0 36.0 26.0 37.0 88-89 32.65299315593765 35.0 34.0 35.0 26.0 36.5 90-91 32.462623069353214 35.0 34.0 35.0 25.0 36.0 92-93 32.29748569910002 35.0 34.0 35.0 25.0 36.0 94-95 32.14746502594431 35.0 33.5 35.0 25.0 36.0 96-97 32.01204776207187 35.0 33.0 35.0 24.0 35.5 98-99 31.86971999846216 35.0 33.0 35.0 24.0 35.0 100-101 30.297512341502994 34.0 30.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15896999251389587 1101 2 0.15928927469920495 1101 3 0.34095036002601375 1101 4 0.11482214133111057 1101 5 0.26168965092332286 1101 6 0.1792323324714289 1101 7 0.1998598670773788 1101 8 0.18482493130674982 1101 9 0.4110191332729016 1101 10-11 0.4580962750567963 1101 12-13 0.5383459663316117 1101 14-15 0.6360607913745469 1101 16-17 0.5530509463903499 1101 18-19 0.6647602773076642 1101 20-21 0.6182525996829966 1101 22-23 0.653825829375215 1101 24-25 0.735631109151889 1101 26-27 0.7303134612917646 1101 28-29 0.7177345394098822 1101 30-31 0.7079640267869678 1101 32-33 0.7597292489773793 1101 34-35 0.7404568482487903 1101 36-37 0.6942403982544292 1101 38-39 0.7982773400797214 1101 40-41 0.8120089891583788 1101 42-43 0.8618103487246245 1101 44-45 0.8737729950277497 1101 46-47 0.974383114454767 1101 48-49 0.7931838491121894 1101 50-51 0.6977008114163681 1101 52-53 0.671969682874753 1101 54-55 0.801095792014209 1101 56-57 0.733798208131546 1101 58-59 0.758959411536992 1101 60-61 0.6634838915860968 1101 62-63 0.7931749711062253 1101 64-65 0.8290707179744246 1101 66-67 0.8807394491469722 1101 68-69 0.7676383922320511 1101 70-71 0.7164336364411241 1101 72-73 0.6404792601344127 1101 74-75 0.4824025991000056 1101 76-77 0.5390056370627931 1101 78-79 0.57291045274075 1101 80-81 0.547186199982221 1101 82-83 0.527449001226806 1101 84-85 0.44873137923336515 1101 86-87 0.5103705829128344 1101 88-89 0.5149838330145755 1101 90-91 0.42225930437569303 1101 92-93 0.5891098446163525 1101 94-95 0.5266500879353089 1101 96-97 0.580467495583342 1101 98-99 0.6563435073988195 1101 100-101 0.5910900953390197 1102 1 0.06140692649098156 1102 2 0.010178841307784126 1102 3 0.12341900557068186 1102 4 0.03057579101827912 1102 5 0.05857134677796694 1102 6 0.09855174795010413 1102 7 0.0858839221583736 1102 8 0.20398400338628875 1102 9 0.20241737056684883 1102 10-11 0.2644039486847731 1102 12-13 0.3349919681028908 1102 14-15 0.3609209819296453 1102 16-17 0.33159582111537844 1102 18-19 0.39454772409794003 1102 20-21 0.5346570864412783 1102 22-23 0.3635730296151962 1102 24-25 0.3708230926961562 1102 26-27 0.3823700707252442 1102 28-29 0.43160727888935213 1102 30-31 0.4243561931727058 1102 32-33 0.37637907184970487 1102 34-35 0.2893098037097488 1102 36-37 0.37522488529840103 1102 38-39 0.3752912745996184 1102 40-41 0.49805822065281546 1102 42-43 0.462487433545931 1102 44-45 0.3911554336758698 1102 46-47 0.5673349685815552 1102 48-49 0.5203670548374149 1102 50-51 0.4360535954634486 1102 52-53 0.5522977976077712 1102 54-55 0.43277825494595845 1102 56-57 0.4242756314952558 1102 58-59 0.4726625226988972 1102 60-61 0.6235694556806024 1102 62-63 0.6128600695263557 1102 64-65 0.44656868243462355 1102 66-67 0.45411798098709966 1102 68-69 0.5403944774247478 1102 70-71 0.4550051517398117 1102 72-73 0.5214662469641098 1102 74-75 0.558858545733159 1102 76-77 0.42659027266545735 1102 78-79 0.5438397302312481 1102 80-81 0.5249140018849161 1102 82-83 0.4959400948473487 1102 84-85 0.4950845489616782 1102 86-87 0.527925328220384 1102 88-89 0.49903162891777697 1102 90-91 0.46959006888159394 1102 92-93 0.5003701294490739 1102 94-95 0.47319931228750534 1102 96-97 0.5095521454704866 1102 98-99 0.6188761831694656 1102 100-101 0.5452547383696142 1103 1 0.15069080196151674 1103 2 0.19914621582294956 1103 3 0.2564752635082357 1103 4 0.08819097848753188 1103 5 0.11740648596401826 1103 6 0.1398062816510901 1103 7 0.1397906280312995 1103 8 0.07140685896002452 1103 9 0.12627479210664916 1103 10-11 0.1260981798720593 1103 12-13 0.18347196261095888 1103 14-15 0.22316118799135864 1103 16-17 0.22565917565365368 1103 18-19 0.2933789731683163 1103 20-21 0.2802953657515772 1103 22-23 0.2384418097206904 1103 24-25 0.29935508889757756 1103 26-27 0.21865841410804165 1103 28-29 0.16054559195213614 1103 30-31 0.33781113075923486 1103 32-33 0.2946519606038436 1103 34-35 0.39390528888726095 1103 36-37 0.30260271850396236 1103 38-39 0.3119492504809003 1103 40-41 0.30278192232670875 1103 42-43 0.21557664305154134 1103 44-45 0.3327514966499763 1103 46-47 0.419524517328135 1103 48-49 0.40022219093425804 1103 50-51 0.3408952162686987 1103 52-53 0.39054764600767555 1103 54-55 0.32172868171453217 1103 56-57 0.33448153588993534 1103 58-59 0.3823975371724089 1103 60-61 0.3555419159499351 1103 62-63 0.3281858381102438 1103 64-65 0.30163791791247263 1103 66-67 0.3462970607459184 1103 68-69 0.32073058627933904 1103 70-71 0.20615323642991257 1103 72-73 0.2344207612145155 1103 74-75 0.3859050040116685 1103 76-77 0.42484401118103676 1103 78-79 0.3754370517721881 1103 80-81 0.38236736340251554 1103 82-83 0.40974221095586216 1103 84-85 0.33473741334537266 1103 86-87 0.2886041963111339 1103 88-89 0.37916028877292973 1103 90-91 0.3962538799363884 1103 92-93 0.3635721380073349 1103 94-95 0.22884927624672002 1103 96-97 0.2499927582781183 1103 98-99 0.29064946093517463 1103 100-101 0.22156994071283975 1104 1 0.21921245531610367 1104 2 0.20753143513159245 1104 3 0.29909261242922014 1104 4 0.16681715222902227 1104 5 0.13487700682520654 1104 6 0.14486768706304076 1104 7 0.14549156803361996 1104 8 0.21926044464252215 1104 9 0.35337201145566155 1104 10-11 0.30942953699725706 1104 12-13 0.46144087895932984 1104 14-15 0.42883116039171654 1104 16-17 0.41540574588136536 1104 18-19 0.45614557205524875 1104 20-21 0.48660518809467845 1104 22-23 0.46865187223614413 1104 24-25 0.4705409651482597 1104 26-27 0.3790731150783202 1104 28-29 0.5005645004797614 1104 30-31 0.45746243934539876 1104 32-33 0.4580019613606865 1104 34-35 0.4490235207948672 1104 36-37 0.5224014227841778 1104 38-39 0.4000129743634133 1104 40-41 0.406741297374019 1104 42-43 0.4862331688271837 1104 44-45 0.4197017113418582 1104 46-47 0.5282608499567658 1104 48-49 0.48765174121655264 1104 50-51 0.33487040939048995 1104 52-53 0.45277675832861775 1104 54-55 0.47499623674983127 1104 56-57 0.3767923623149585 1104 58-59 0.5565243895521874 1104 60-61 0.49041181417941715 1104 62-63 0.38584581005650165 1104 64-65 0.41640126488584883 1104 66-67 0.46905269798474336 1104 68-69 0.4097655954371717 1104 70-71 0.38626389988083076 1104 72-73 0.3914470010538622 1104 74-75 0.3569569185789234 1104 76-77 0.4097118280849301 1104 78-79 0.27754631758143233 1104 80-81 0.3804575894975457 1104 82-83 0.3282573680518013 1104 84-85 0.3397120968577454 1104 86-87 0.3702646904809015 1104 88-89 0.48303893361552497 1104 90-91 0.37662193398525545 1104 92-93 0.4548442568220139 1104 94-95 0.3404572074847749 1104 96-97 0.3775426974781837 1104 98-99 0.27262202780248757 1104 100-101 0.31044916580378157 1105 1 0.28373332055886635 1105 2 0.2339395218813891 1105 3 0.33679076410981423 1105 4 0.3836446580784312 1105 5 0.2898417224358454 1105 6 0.25903335925180215 1105 7 0.28437745521754465 1105 8 0.27132488681088773 1105 9 0.46059826379307367 1105 10-11 0.377145260397711 1105 12-13 0.498918796851477 1105 14-15 0.5256888059691889 1105 16-17 0.4765126937581883 1105 18-19 0.5075487255681779 1105 20-21 0.48260926623714795 1105 22-23 0.4137737231866083 1105 24-25 0.4432672127873545 1105 26-27 0.3258575843090128 1105 28-29 0.5059411413683108 1105 30-31 0.4465723189744111 1105 32-33 0.366969646310082 1105 34-35 0.3516619803094372 1105 36-37 0.45312115855062274 1105 38-39 0.3819027094720937 1105 40-41 0.4344055976530896 1105 42-43 0.29151302430683756 1105 44-45 0.4081391438035027 1105 46-47 0.4319693520381236 1105 48-49 0.42164033369360254 1105 50-51 0.44013804923600475 1105 52-53 0.3209395269551152 1105 54-55 0.42106891037627747 1105 56-57 0.48241883641311034 1105 58-59 0.5552358750672681 1105 60-61 0.593629224277052 1105 62-63 0.6845996979169726 1105 64-65 0.5067787514721473 1105 66-67 0.5190011339137399 1105 68-69 0.5563267769708844 1105 70-71 0.5286564962668763 1105 72-73 0.6213376006535682 1105 74-75 0.5944546868765386 1105 76-77 0.4594922990446122 1105 78-79 0.5903881204211032 1105 80-81 0.6291115205825761 1105 82-83 0.6242275008263434 1105 84-85 0.6394667270529126 1105 86-87 0.5328747486461154 1105 88-89 0.5221906282998461 1105 90-91 0.5098839311474705 1105 92-93 0.5540639831918028 1105 94-95 0.6951727946339261 1105 96-97 0.5584750322617325 1105 98-99 0.5991870384676723 1105 100-101 0.5872818632977044 1106 1 0.30279402458977955 1106 2 0.2974509433075383 1106 3 0.2509084319247208 1106 4 0.22911909163832433 1106 5 0.1377607690257392 1106 6 0.21903741706684343 1106 7 0.16334871089076586 1106 8 0.14615231940484819 1106 9 0.2002949421186102 1106 10-11 0.2173075950527803 1106 12-13 0.2909265552174105 1106 14-15 0.3038707725098604 1106 16-17 0.26354080735936947 1106 18-19 0.28964791709264404 1106 20-21 0.28797418289756394 1106 22-23 0.16287008747254816 1106 24-25 0.19859585169914595 1106 26-27 0.2671609589115036 1106 28-29 0.2623134547198518 1106 30-31 0.22160396552108352 1106 32-33 0.0876761913011137 1106 34-35 0.1398826284130692 1106 36-37 0.18630052279685572 1106 38-39 0.24328366773393384 1106 40-41 0.12694506210971923 1106 42-43 0.15082151969548363 1106 44-45 0.13070754224565917 1106 46-47 0.19823644889198988 1106 48-49 0.16758582665197252 1106 50-51 0.14407468116131383 1106 52-53 0.18566432329249238 1106 54-55 0.19696670195454402 1106 56-57 0.2304968876033371 1106 58-59 0.2388668917704706 1106 60-61 0.2031130437395703 1106 62-63 0.3225852573882264 1106 64-65 0.26098708057858033 1106 66-67 0.35782979267354875 1106 68-69 0.2972275678037519 1106 70-71 0.4303046528279708 1106 72-73 0.27699174690251027 1106 74-75 0.3443444201634165 1106 76-77 0.3949206631717601 1106 78-79 0.41700474070223237 1106 80-81 0.21934718260187225 1106 82-83 0.26737354247187994 1106 84-85 0.4329669453810041 1106 86-87 0.33123883169017887 1106 88-89 0.26242320285117415 1106 90-91 0.3901375886943512 1106 92-93 0.23942563595751665 1106 94-95 0.23319551398749638 1106 96-97 0.13129792627778158 1106 98-99 0.24982129260157038 1106 100-101 0.4105331434942414 1107 1 -0.028314233687112278 1107 2 0.010970664823894083 1107 3 0.06682297439408558 1107 4 -0.008469892714259686 1107 5 0.021318169655216934 1107 6 0.040344426561901514 1107 7 0.010522472230611868 1107 8 0.07146751576551935 1107 9 0.14831475417063444 1107 10-11 0.07778730183929383 1107 12-13 0.08180010232342738 1107 14-15 0.1252545847327795 1107 16-17 0.03821660829483875 1107 18-19 -0.0388162419734428 1107 20-21 0.06595377269979252 1107 22-23 0.14659833602448202 1107 24-25 0.1279009752040423 1107 26-27 0.09372183448294891 1107 28-29 0.04542407709660523 1107 30-31 0.10408968835173482 1107 32-33 0.1065696623571597 1107 34-35 0.21884549658296493 1107 36-37 0.19272830076678105 1107 38-39 0.11291358031106569 1107 40-41 0.232517532349938 1107 42-43 0.16350617685014157 1107 44-45 0.036365054976599254 1107 46-47 0.04937826640056642 1107 48-49 0.014760096619156116 1107 50-51 0.04668958516298005 1107 52-53 -0.007690358825591659 1107 54-55 0.005437737662219888 1107 56-57 0.08378384090524094 1107 58-59 -0.08597787516631428 1107 60-61 0.05885615845131298 1107 62-63 0.05464291945215649 1107 64-65 0.19117365525830365 1107 66-67 0.20333322183905267 1107 68-69 0.06318320736193783 1107 70-71 0.137746531982188 1107 72-73 0.23167788314546556 1107 74-75 0.18040314831024062 1107 76-77 0.21211974398043765 1107 78-79 0.1207104977533362 1107 80-81 0.17388708848546486 1107 82-83 0.13147798197471872 1107 84-85 0.16423623507275664 1107 86-87 0.17128316364886587 1107 88-89 0.17251162262741815 1107 90-91 0.2795711609468938 1107 92-93 0.25435842594121993 1107 94-95 0.10264740699672359 1107 96-97 0.07187830058681044 1107 98-99 0.10014854343012658 1107 100-101 0.34722022985305756 1108 1 0.09528407299653452 1108 2 0.024676687655151852 1108 3 0.052953587895480325 1108 4 -0.18503485526809982 1108 5 0.07963616600455481 1108 6 0.047282188781835544 1108 7 0.09298335229807009 1108 8 0.17575066560588226 1108 9 0.223369027735842 1108 10-11 0.11486684105931744 1108 12-13 0.139338344058217 1108 14-15 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-0.39989945240963465 2316 86-87 -0.3666578146530881 2316 88-89 -0.30829202905439246 2316 90-91 -0.1903167605330296 2316 92-93 -0.22706662597187943 2316 94-95 -0.19926269096936267 2316 96-97 -0.2534574537067229 2316 98-99 -0.1763136934125349 2316 100-101 -0.05394033062447079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 27.0 7 743.0 8 1879.0 9 1444.0 10 1465.0 11 1783.0 12 2144.0 13 2165.0 14 2239.0 15 2298.0 16 2709.0 17 3205.0 18 4080.0 19 5231.0 20 6546.0 21 8477.0 22 9616.0 23 9790.0 24 9362.0 25 8849.0 26 9160.0 27 10142.0 28 11308.0 29 13238.0 30 16132.0 31 19556.0 32 25091.0 33 33760.0 34 48845.0 35 78645.0 36 148509.0 37 324664.0 38 484478.0 39 174801.0 40 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.82452638913152 19.641656307605764 13.800899636381786 23.73291766688093 2 15.01019493470588 21.321544126226986 40.44485393555507 23.223407003512076 3 17.47127847786219 26.736224697305204 31.168373916345555 24.624122908487056 4 12.29927984573037 18.2305532379288 39.45881522920223 30.0113516871386 5 13.002032242029033 38.08784438678294 35.66163203941187 13.248491331776158 6 29.353250609234188 38.46596416158381 18.961094672261346 13.219690556920652 7 25.703039055064785 33.50528362692812 23.723946933729216 17.06773038427788 8 24.69905888240853 35.5289596175365 21.62998895857882 18.141992541476153 9 26.131745670946767 16.02294349314953 21.043451398523807 36.801859437379896 10-11 23.504298869755072 26.540993215292875 30.244219778915273 19.710488136036783 12-13 24.70192547006979 24.72681419588169 29.296896868269844 21.274363465778677 14-15 22.238717279588073 26.096031361143506 26.926161423284384 24.739089935984037 16-17 21.488817947401824 28.89253938522824 27.677848157188826 21.940794510181107 18-19 21.482781251032815 28.13070155854685 29.344752019932564 21.04176517048777 20-21 22.544632769885855 27.43044477974152 29.063624081764523 20.9612983686081 22-23 22.315305756984863 27.274536135529566 28.577551988096577 21.83260611938899 24-25 21.944032068010298 27.573470641758156 28.743611725085476 21.73888556514607 26-27 21.799825846368435 27.72502881763714 28.737001711184213 21.738143624810217 28-29 21.871591711582163 27.57188558740428 28.931491252860685 21.625031448152875 30-31 22.23935804624177 27.442113477750894 28.766578150936294 21.55195032507104 32-33 21.984332918071576 27.64176287721764 28.65734429876184 21.716559905948944 34-35 22.16924468450336 27.663582668003954 28.562713181379458 21.604459466113223 36-37 22.167457282785165 27.55107753343959 28.560824605979096 21.720640577796154 38-39 22.001802240524913 27.757235773125437 28.56288180418306 21.678080182166585 40-41 22.058560676217912 27.574549827701215 28.709718541561134 21.657170954519735 42-43 22.009525164929965 27.505954071195244 28.6088146558847 21.87570610799009 44-45 22.077446430221524 27.682097451839642 28.33645510350405 21.904001014434787 46-47 22.086750479058033 27.647297276333354 28.20513598772964 22.060816256878976 48-49 21.95112080583086 27.741782308575374 28.318069545674675 21.989027339919087 50-51 21.89860508471947 27.92123831191037 28.069896175567365 22.110260427802796 52-53 21.98038714446725 27.846605859035385 27.998433831400128 22.17457316509724 54-55 21.9414352768348 27.77170360967463 28.07650618946863 22.21035492402194 56-57 21.973271262155176 27.894292387894502 27.944575707929115 22.187860642021207 58-59 21.938298892687776 27.69400222177406 28.08503850333097 22.282660382207194 60-61 21.945246152196244 27.745938045283992 28.127801246324868 22.181014556194896 62-63 21.819048845979257 27.67963555890703 28.200949818528137 22.300365776585576 64-65 21.824107530087367 27.595998648319608 28.111512283496754 22.468381538096274 66-67 21.87887621669784 27.772512999131933 27.859185120184215 22.48942566398601 68-69 21.952260860826158 27.770995393899494 27.81031823169987 22.46642551357447 70-71 22.010705524555192 27.46292153171559 27.849742243182412 22.67663070054681 72-73 21.980454593588693 27.55812596663022 27.852035513311424 22.609383926469665 74-75 22.013774459580777 27.642808338599984 27.738383743682547 22.605033458136692 76-77 21.945313601317686 27.678623822085406 27.778347348136617 22.59771522846029 78-79 22.007333068483117 27.69474416210992 27.60169809908141 22.696224670325556 80-81 22.05394041139917 27.771872232478234 27.530235754914173 22.643951601208418 82-83 22.12044524514046 27.62618213016466 27.678050504553152 22.575322120141724 84-85 22.195651015547696 27.513811893843176 27.68482914125802 22.605707949351107 86-87 22.077513879342963 27.553539426372204 27.707761842548116 22.661184851736714 88-89 22.192244834914902 27.430040085012873 27.61903252329187 22.758682556780357 90-91 22.31564300259207 27.322661083678057 27.681861379914597 22.67983453381528 92-93 22.50932315481125 27.186616205730612 27.574414929458335 22.729645709999804 94-95 22.376448385571553 27.06031772583146 27.67150793977333 22.891725948823655 96-97 22.37176067163137 27.071986423840837 27.633534084401784 22.922718820126008 98-99 22.51583199503035 27.008112105835764 27.483324890951632 22.99273100818225 100-101 22.872570398334275 26.86434430348327 27.433682337570716 22.829402960611738 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 77.5 2 67.0 3 94.0 4 82.0 5 95.0 6 156.5 7 283.0 8 515.0 9 691.5 10 678.5 11 621.5 12 686.0 13 798.0 14 868.0 15 966.0 16 1088.0 17 1225.0 18 1335.0 19 1472.0 20 1722.5 21 2052.5 22 2512.5 23 3229.5 24 4102.0 25 5341.5 26 7451.0 27 10117.0 28 12914.0 29 16758.0 30 21302.0 31 25171.5 32 28798.5 33 32452.5 34 35464.0 35 37677.5 36 41421.0 37 45151.5 38 46427.0 39 47787.5 40 49431.5 41 50314.0 42 50798.5 43 52198.0 44 54153.0 45 55447.5 46 56313.0 47 56741.0 48 56999.0 49 57030.0 50 56164.5 51 54165.5 52 51163.5 53 48206.0 54 45202.0 55 41432.0 56 36638.5 57 31923.5 58 28266.5 59 24623.0 60 20550.0 61 16694.5 62 13379.0 63 9965.5 64 6932.5 65 4832.0 66 3572.5 67 2699.5 68 1915.0 69 1379.0 70 1019.5 71 741.0 72 520.5 73 367.5 74 264.0 75 191.0 76 139.5 77 99.0 78 78.5 79 65.0 80 51.0 81 37.0 82 30.5 83 27.0 84 23.5 85 21.0 86 17.0 87 13.5 88 13.0 89 10.5 90 7.5 91 5.5 92 6.0 93 5.0 94 2.5 95 2.0 96 0.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.3489824288293734E-4 48-49 3.3724560720734334E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1482599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.223785365410464 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64638208323268 42.431699547055615 2 15.591616563176519 18.4678910567469 3 5.818197288946186 10.337270023624859 4 2.743135989611216 6.498355883074701 5 1.3898606627055838 4.115640478795132 6 0.8377167249564532 2.9767653309501343 7 0.5190679238872083 2.151881712005567 8 0.332724594295242 1.5764167966667753 9 0.24679449440833814 1.3154493749583973 >10 0.8419940537823449 7.954950657467756 >50 0.02460257051878392 0.9827919507934457 >100 0.007563291713011036 0.6880952738162565 >500 0.0 0.0 >1k 3.43758766409091E-4 0.5027919140444722 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3369 0.22723609013630794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.1762445543265576 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.3724560720734334E-5 0.0 18-19 0.0 0.0 0.0 1.01173682162203E-4 0.0 20-21 0.0 0.0 0.0 2.3607192504514034E-4 0.0 22-23 0.0 0.0 0.0 6.744912144146867E-4 0.0 24-25 0.0 0.0 0.0 0.0013827069895501077 0.0 26-27 0.0 0.0 0.0 0.0024956174933343407 0.0 28-29 0.0 0.0 0.0 0.00886955946955313 0.0 30-31 0.0 0.0 0.0 0.0303521046486609 0.0 32-33 0.0 0.0 0.0 0.07746531597552675 0.0 34-35 0.0 0.0 0.0 0.15014174432870925 0.0 36-37 0.0 0.0 0.0 0.2686498507013697 0.0 38-39 0.0 0.0 0.0 0.4309324368895433 0.0 40-41 3.3724560720734334E-5 0.0 0.0 0.5963851317854659 0.0 42-43 6.744912144146867E-5 0.0 0.0 0.7647044143426509 0.0 44-45 6.744912144146867E-5 0.0 0.0 0.9690078031888596 0.0 46-47 6.744912144146867E-5 0.0 0.0 1.2044726861410266 0.0 48-49 6.744912144146867E-5 0.0 0.0 1.453359944260046 0.0 50-51 6.744912144146867E-5 0.0 0.0 1.6851488500936531 0.0 52-53 6.744912144146867E-5 0.0 0.0 1.9103614665867168 0.0 54-55 6.744912144146867E-5 0.0 0.0 2.1605302580131243 0.0 56-57 6.744912144146867E-5 0.0 0.0 2.4553840923945045 0.0 58-59 6.744912144146867E-5 0.0 0.0 2.7616705528602137 0.0 60-61 6.744912144146867E-5 0.0 0.0 3.0304215772437457 0.0 62-63 6.744912144146867E-5 0.0 0.0 3.283119710724208 0.0 64-65 6.744912144146867E-5 0.0 0.0 3.593250771112081 0.0 66-67 6.744912144146867E-5 0.0 0.0 3.9773397931605246 0.0 68-69 6.744912144146867E-5 0.0 0.0 4.401527317905921 0.0 70-71 6.744912144146867E-5 0.0 0.0 4.825208974240506 0.0 72-73 6.744912144146867E-5 0.0 0.0 5.2403245921520245 0.0 74-75 6.744912144146867E-5 0.0 0.0 5.675270251767335 0.0 76-77 6.744912144146867E-5 0.0 0.0 6.1319345284867985 0.0 78-79 6.744912144146867E-5 0.0 0.0 6.634396758665019 0.0 80-81 6.744912144146867E-5 0.0 0.0 7.1428956852122525 0.0 82-83 6.744912144146867E-5 0.0 0.0 7.6556439064102975 0.0 84-85 6.744912144146867E-5 0.0 0.0 8.176317399377714 0.0 86-87 6.744912144146867E-5 0.0 0.0 8.758808012146238 0.0 88-89 6.744912144146867E-5 0.0 0.0 9.397416293954063 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 52.858974 1 GTATCAA 3610 0.0 44.605263 1 TCAACGC 4285 0.0 36.802803 4 TATCAAC 4455 0.0 35.824917 2 ATCAACG 4405 0.0 35.800224 3 CAACGCA 4410 0.0 35.651928 5 AACGCAG 4545 0.0 34.697468 6 ACGCAGA 5085 0.0 30.91937 7 CGCAGAG 5230 0.0 29.880497 8 GTACATG 4390 0.0 25.318909 1 GCAGAGT 6180 0.0 24.672329 9 GTATTAC 330 0.0 24.469698 1 TACATGG 4415 0.0 23.66931 2 TCGCCGG 230 0.0 21.684782 94-95 CAGAGTA 5610 0.0 21.548574 10-11 GAGTACT 3565 0.0 21.451614 12-13 ACATGGG 4550 0.0 21.401098 3 GTATAGA 500 0.0 20.9 1 AGAGTAC 5380 0.0 20.836432 10-11 GTACTTT 3850 0.0 19.67857 14-15 >>END_MODULE