FastQCFastQC Report
Fri 10 Feb 2017
SRR2031500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482599
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT62720.42304088968089143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.28139773465380724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.22696629365054205No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT20130.13577508146167644RNA PCR Primer, Index 17 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18520.12491577290959996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.056.64171
GTATCAA40550.042.940281
TCAACGC46900.036.5611724
CAACGCA47750.035.810875
ATCAACG48250.035.4397743
AACGCAG49350.034.9385766
TATCAAC50450.033.988482
ACGCAGA55650.030.9832677
CGCAGAG56650.030.3524958
GCAGAGT66950.025.2571969
GTACATG40600.023.7872751
TACATGG40350.023.7790012
GAGTACT41600.022.43649512-13
ACATGGG41350.021.8254813
CAGAGTA62400.021.80849810-11
AGAGTAC58400.021.51288210-11
GTACTTT45650.020.39393614-15
GTATAGA6000.019.029821
AGTACTT45450.019.02055512-13
CATGGGG24700.018.26894
Produced by FastQC (version 0.11.5)