FastQCFastQC Report
Fri 10 Feb 2017
SRR2031499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515374
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.18688455787152214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.14095530212343618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.049.961141
GTATCAA34650.041.673881
CAACGCA41500.034.451815
TCAACGC41300.034.388624
ATCAACG41750.034.1317373
AACGCAG42100.033.9608086
TATCAAC44150.032.5990942
ACGCAGA48250.029.2383447
CGCAGAG48700.028.5780288
GTACATG41000.028.2682931
CCTACGC908.948867E-426.3888913
TACATGG42950.025.547152
ACATGGG42750.024.6666663
GCAGAGT57500.023.7086969
CATGGGG23650.022.0930214
GAGTACT33650.021.10326812-13
AGAGTAC50750.020.49753610-11
CAGAGTA54650.020.0343110-11
AGTACTT39650.017.7900412-13
GTACTTT39550.017.7149214-15
Produced by FastQC (version 0.11.5)