##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031499_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1515374 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21612420432184 34.0 31.0 34.0 30.0 34.0 2 32.382619736117945 34.0 31.0 34.0 31.0 34.0 3 32.48684813122041 34.0 31.0 34.0 31.0 34.0 4 35.706519314703826 37.0 35.0 37.0 35.0 37.0 5 35.69055031959107 37.0 35.0 37.0 35.0 37.0 6 35.813228945461645 37.0 37.0 37.0 35.0 37.0 7 35.837642060639816 37.0 37.0 37.0 35.0 37.0 8 35.8858816371404 37.0 37.0 37.0 35.0 37.0 9 37.713200173686495 39.0 39.0 39.0 35.0 39.0 10-11 37.71594339087248 39.0 39.0 39.0 35.0 39.0 12-13 37.695466927636346 39.0 39.0 39.0 35.0 39.0 14-15 39.230703773457904 41.0 40.0 41.0 36.0 41.0 16-17 39.23794324041458 41.0 40.0 41.0 36.0 41.0 18-19 39.19709226897122 41.0 40.0 41.0 36.0 41.0 20-21 39.15909834799858 41.0 40.0 41.0 36.0 41.0 22-23 39.09367027545675 41.0 39.0 41.0 36.0 41.0 24-25 39.0435516248794 41.0 39.0 41.0 36.0 41.0 26-27 38.94788217298172 41.0 39.0 41.0 35.5 41.0 28-29 38.84648674188682 41.0 39.0 41.0 35.0 41.0 30-31 38.68842246204568 41.0 39.0 41.0 35.0 41.0 32-33 38.586864364836664 40.0 38.5 41.0 35.0 41.0 34-35 38.4572125429102 40.0 38.0 41.0 34.5 41.0 36-37 38.32034039121696 40.0 38.0 41.0 34.0 41.0 38-39 38.179849660875796 40.0 38.0 41.0 34.0 41.0 40-41 38.059357293974955 40.0 38.0 41.0 33.0 41.0 42-43 37.914755697273414 40.0 38.0 41.0 33.0 41.0 44-45 38.073631327975804 40.0 38.0 41.0 33.0 41.0 46-47 37.957040968104245 40.0 38.0 41.0 33.0 41.0 48-49 37.8382897555323 40.0 38.0 41.0 33.0 41.0 50-51 36.79582532100986 39.0 36.5 40.0 31.5 40.5 52-53 36.86603505141305 39.5 37.0 40.0 31.5 40.5 54-55 37.64640940124352 40.0 38.0 41.0 33.0 41.0 56-57 37.68324618213062 40.0 37.5 41.0 33.0 41.0 58-59 37.50610212396411 40.0 37.0 41.0 32.0 41.0 60-61 37.34811505278565 40.0 37.0 41.0 32.0 41.0 62-63 37.1050397459637 40.0 36.0 41.0 31.5 41.0 64-65 36.841262289045474 39.0 36.0 41.0 31.0 41.0 66-67 36.55637651167302 39.0 35.0 41.0 31.0 41.0 68-69 36.26049410904503 38.5 35.0 41.0 31.0 41.0 70-71 35.89492362941426 37.5 35.0 40.5 30.0 41.0 72-73 35.51498408973626 37.0 35.0 39.5 30.0 41.0 74-75 35.1150748924028 36.5 35.0 39.0 29.0 41.0 76-77 34.7324449277868 36.0 35.0 39.0 29.0 41.0 78-79 34.31367833947263 35.5 35.0 37.5 29.0 39.5 80-81 33.94973650069224 35.0 34.0 37.0 28.0 39.0 82-83 33.622571721568406 35.0 34.0 37.0 28.0 39.0 84-85 33.30268633353879 35.0 34.0 36.0 27.0 37.5 86-87 33.02155144538576 35.0 34.0 36.0 26.5 37.0 88-89 32.78304695738478 35.0 34.0 35.5 26.0 37.0 90-91 32.58508790569193 35.0 34.0 35.0 26.0 36.0 92-93 32.406496350075955 35.0 34.0 35.0 25.0 36.0 94-95 32.25379048340542 35.0 34.0 35.0 25.0 36.0 96-97 32.12487412348371 35.0 33.0 35.0 25.0 36.0 98-99 31.98044872090982 35.0 33.0 35.0 24.0 35.0 100-101 30.38899868943244 34.0 30.0 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.005387862363157581 1101 2 -0.019119456877845664 1101 3 0.2104696545849194 1101 4 -0.037898924985434235 1101 5 -0.1398329581405804 1101 6 -0.12354317936897274 1101 7 -0.1857792211915097 1101 8 0.08964633451897441 1101 9 0.23239734300177872 1101 10-11 0.2244477851309199 1101 12-13 0.2872149179282246 1101 14-15 0.39790904604164723 1101 16-17 0.27033120989817405 1101 18-19 0.3717167565774062 1101 20-21 0.3662756791376651 1101 22-23 0.4584118809751061 1101 24-25 0.3749538287963077 1101 26-27 0.392473868926011 1101 28-29 0.5391998873095858 1101 30-31 0.48214066710702497 1101 32-33 0.5727817763052201 1101 34-35 0.566623695347289 1101 36-37 0.4804184733266794 1101 38-39 0.6224443387994967 1101 40-41 0.6510164732454058 1101 42-43 0.5813190467555458 1101 44-45 0.6286435010998161 1101 46-47 0.7020313450470255 1101 48-49 0.7489286610371195 1101 50-51 0.6385828064179861 1101 52-53 0.7179445518343854 1101 54-55 0.7242042722126953 1101 56-57 0.6649259040561404 1101 58-59 0.6771999114840952 1101 60-61 0.6832781322956762 1101 62-63 0.6692367368453773 1101 64-65 0.7507740069357851 1101 66-67 0.7561278364254989 1101 68-69 0.8376847262577982 1101 70-71 0.8187255897291976 1101 72-73 0.6649361033154548 1101 74-75 0.7189550286088888 1101 76-77 0.6959886474734773 1101 78-79 0.6236161369113944 1101 80-81 0.6249553573936453 1101 82-83 0.6528238044037167 1101 84-85 0.618333995436501 1101 86-87 0.6794650155144168 1101 88-89 0.5931646116107672 1101 90-91 0.44417228860768176 1101 92-93 0.5679022549139248 1101 94-95 0.48476649076449263 1101 96-97 0.5857480059089468 1101 98-99 0.5824169013874112 1101 100-101 0.5108034308734233 1102 1 0.0773045736910376 1102 2 0.1564831523941379 1102 3 0.26346771697531324 1102 4 0.033454896481757146 1102 5 0.0934785466939303 1102 6 0.11348736255919079 1102 7 0.1338272632996791 1102 8 0.2573042687290439 1102 9 0.3141138875239875 1102 10-11 0.34707175567671555 1102 12-13 0.43109947635301893 1102 14-15 0.47132970394097384 1102 16-17 0.48459840657982056 1102 18-19 0.5103371056008399 1102 20-21 0.5987987417658971 1102 22-23 0.5775035194444271 1102 24-25 0.55343946479843 1102 26-27 0.660049113822744 1102 28-29 0.5775576601050432 1102 30-31 0.5764814041597646 1102 32-33 0.5385909740841015 1102 34-35 0.6009738508951656 1102 36-37 0.7342921069313633 1102 38-39 0.6367005155247156 1102 40-41 0.5183139993841124 1102 42-43 0.7285888723706861 1102 44-45 0.6599167896767995 1102 46-47 0.5975181575682385 1102 48-49 0.5288767800336203 1102 50-51 0.6126058357485107 1102 52-53 0.6151846473512492 1102 54-55 0.5854055425996165 1102 56-57 0.5248123112966496 1102 58-59 0.5946901679398806 1102 60-61 0.5139014020039596 1102 62-63 0.5791081601163555 1102 64-65 0.6552163178602868 1102 66-67 0.4850662420042937 1102 68-69 0.4432444131958988 1102 70-71 0.6058798881464398 1102 72-73 0.49036453385167533 1102 74-75 0.4467371315654063 1102 76-77 0.4124352585593911 1102 78-79 0.350806809112882 1102 80-81 0.33241753278061026 1102 82-83 0.31983084766220315 1102 84-85 0.5369012742525001 1102 86-87 0.5476492913843529 1102 88-89 0.5535843371742146 1102 90-91 0.6185044316800159 1102 92-93 0.5476524903763007 1102 94-95 0.5597917811681015 1102 96-97 0.5150859047283021 1102 98-99 0.5718789689532215 1102 100-101 0.436297822587683 1103 1 0.19224780441534506 1103 2 0.2177502011488528 1103 3 0.3269257601323048 1103 4 0.2315598517298696 1103 5 0.06958182116178335 1103 6 0.11394859105560329 1103 7 0.21081743245284912 1103 8 0.30038364647136717 1103 9 0.4381360035894346 1103 10-11 0.39078635638961146 1103 12-13 0.40276286988780186 1103 14-15 0.5111690310181416 1103 16-17 0.5085911948746684 1103 18-19 0.49825101046307196 1103 20-21 0.4827778268030656 1103 22-23 0.44539972585594256 1103 24-25 0.46632930625669644 1103 26-27 0.451005727171399 1103 28-29 0.3672724554881839 1103 30-31 0.411810425483786 1103 32-33 0.5162706957438346 1103 34-35 0.5077764304666204 1103 36-37 0.42252359147002494 1103 38-39 0.615068899042889 1103 40-41 0.6555902319934077 1103 42-43 0.6476563128833419 1103 44-45 0.6712273847633483 1103 46-47 0.588921812660935 1103 48-49 0.6345426381190222 1103 50-51 0.6045696448978859 1103 52-53 0.6244594752812134 1103 54-55 0.6459823390528001 1103 56-57 0.6542339816563256 1103 58-59 0.6407726203267856 1103 60-61 0.5944124940721665 1103 62-63 0.6784672751683658 1103 64-65 0.6061470133483269 1103 66-67 0.6807180978287235 1103 68-69 0.7603698590766257 1103 70-71 0.6193331281652945 1103 72-73 0.5419104763450733 1103 74-75 0.48845477193314224 1103 76-77 0.4265186321309429 1103 78-79 0.4979526953682125 1103 80-81 0.4456173197884823 1103 82-83 0.598028608445567 1103 84-85 0.3791999352902735 1103 86-87 0.4329159991480722 1103 88-89 0.45948259080407894 1103 90-91 0.46845026118833744 1103 92-93 0.3375495505376733 1103 94-95 0.5090426506633392 1103 96-97 0.38652593643538324 1103 98-99 0.3080899863978388 1103 100-101 0.23624775543917664 1104 1 0.1365792344773169 1104 2 0.1564356170991843 1104 3 0.21449216431577867 1104 4 0.026243113161214637 1104 5 0.053812503747955986 1104 6 0.008828841919303443 1104 7 0.046682720814551715 1104 8 0.14294793057653976 1104 9 0.20870344494569792 1104 10-11 0.1871577760403369 1104 12-13 0.2682657801257804 1104 14-15 0.2573801320981701 1104 16-17 0.2351871960287113 1104 18-19 0.2967888057284398 1104 20-21 0.2816281289495137 1104 22-23 0.36739072498024683 1104 24-25 0.19701951454381117 1104 26-27 0.24305314691471125 1104 28-29 0.3070176282872623 1104 30-31 0.3670973813280014 1104 32-33 0.2881957211932402 1104 34-35 0.22472057049680672 1104 36-37 0.41017547630714546 1104 38-39 0.31602155100394924 1104 40-41 0.3116635646830943 1104 42-43 0.22494347085486055 1104 44-45 0.31428580802356976 1104 46-47 0.5542366541688466 1104 48-49 0.6287515214447055 1104 50-51 0.4478509727952371 1104 52-53 0.28902554239764555 1104 54-55 0.4567657743249214 1104 56-57 0.3548992514990985 1104 58-59 0.41299236771282466 1104 60-61 0.40350438351642737 1104 62-63 0.4822188821651352 1104 64-65 0.4389338125156499 1104 66-67 0.39362562006977697 1104 68-69 0.3162103501443454 1104 70-71 0.22537374220321027 1104 72-73 0.3646617677296504 1104 74-75 0.4379946500131169 1104 76-77 0.4689173751023574 1104 78-79 0.4178078073540803 1104 80-81 0.40253456220743544 1104 82-83 0.4252357341670532 1104 84-85 0.4340577376018615 1104 86-87 0.3954397335798916 1104 88-89 0.6154178784487456 1104 90-91 0.4529022675276835 1104 92-93 0.3028626982413627 1104 94-95 0.30235760057472305 1104 96-97 0.3846904172476755 1104 98-99 0.5160092551712623 1104 100-101 0.2755942631818371 1105 1 0.1650699729993974 1105 2 0.14046944714949916 1105 3 0.24372042696615637 1105 4 0.09174187319210603 1105 5 0.12331054439930256 1105 6 0.13468984263601413 1105 7 0.07006949351676184 1105 8 0.12913509626918085 1105 9 0.22967858846138256 1105 10-11 0.2432779188275731 1105 12-13 0.30302200492014464 1105 14-15 0.26816201974367715 1105 16-17 0.30379615187705866 1105 18-19 0.2889263288961388 1105 20-21 0.381709939693998 1105 22-23 0.437186817169696 1105 24-25 0.3077919563303695 1105 26-27 0.24297149626953995 1105 28-29 0.35446198977654575 1105 30-31 0.23324235551732642 1105 32-33 0.308126750412967 1105 34-35 0.3658686532809483 1105 36-37 0.44469635512425043 1105 38-39 0.44439504346887304 1105 40-41 0.41386322239078055 1105 42-43 0.4167178888004983 1105 44-45 0.4298190694545241 1105 46-47 0.494233035924573 1105 48-49 0.3706167423790774 1105 50-51 0.3365947642776277 1105 52-53 0.29477284058847175 1105 54-55 0.3691258141789717 1105 56-57 0.36954593634948907 1105 58-59 0.3410328002584464 1105 60-61 0.2912819756976077 1105 62-63 0.33708299254400487 1105 64-65 0.3416411984835932 1105 66-67 0.38573779418097587 1105 68-69 0.27665461504038547 1105 70-71 0.25610000067712946 1105 72-73 0.23183137746624993 1105 74-75 0.2582268793905982 1105 76-77 0.2165931334527329 1105 78-79 0.2927083456613033 1105 80-81 0.3243201817677246 1105 82-83 0.2552441607271092 1105 84-85 0.2670979113202776 1105 86-87 0.21312665085178395 1105 88-89 0.2889132251624602 1105 90-91 0.31364842634512513 1105 92-93 0.35042793470535827 1105 94-95 0.25288558139695994 1105 96-97 0.11989897594302334 1105 98-99 0.22485853074858397 1105 100-101 0.3558573810517487 1106 1 0.11923340213419209 1106 2 0.12718413986779353 1106 3 0.15228269742084422 1106 4 0.12202108973642112 1106 5 0.10292404575501024 1106 6 0.10081604420880552 1106 7 0.11285602676025519 1106 8 0.1320720967247695 1106 9 0.11158030608569192 1106 10-11 0.048047006013135274 1106 12-13 0.1439000776266326 1106 14-15 0.06652804293398873 1106 16-17 0.15188476711771415 1106 18-19 0.21659851521677354 1106 20-21 0.20896211922256924 1106 22-23 0.17057244247901338 1106 24-25 0.1438573488224364 1106 26-27 0.19679212791140088 1106 28-29 0.07649082015149844 1106 30-31 0.09314065155577111 1106 32-33 0.010507755450987588 1106 34-35 -0.052989091411340894 1106 36-37 -0.03665226673153654 1106 38-39 0.07263497124304052 1106 40-41 -0.003622595130863715 1106 42-43 -0.0012746301049659792 1106 44-45 0.04492235839374814 1106 46-47 -0.06994160354830825 1106 48-49 -0.16519343185051838 1106 50-51 -0.20602000161994027 1106 52-53 -0.1636235846848777 1106 54-55 -0.13129316053461793 1106 56-57 -0.09332209177612327 1106 58-59 -0.05261618198930762 1106 60-61 0.06627633612594508 1106 62-63 0.082514202302157 1106 64-65 0.14317521112114662 1106 66-67 0.18660660766915527 1106 68-69 0.10390630310302384 1106 70-71 0.051314989995120186 1106 72-73 0.09643963733770988 1106 74-75 0.07627390186876681 1106 76-77 -0.02583669381173337 1106 78-79 -0.005609268133952128 1106 80-81 -0.09742885704775972 1106 82-83 -0.030007093862572276 1106 84-85 0.052847020906867215 1106 86-87 0.03645472058489929 1106 88-89 -0.07442508672084358 1106 90-91 -0.01568117971051919 1106 92-93 -0.043746670364896545 1106 94-95 -0.03373366863583982 1106 96-97 0.053007758488512025 1106 98-99 -0.028749935047102326 1106 100-101 0.010080621591399108 1107 1 0.15723544621386765 1107 2 0.06814518495131239 1107 3 0.10765195595506327 1107 4 0.12579208000474296 1107 5 0.11674615621722495 1107 6 0.045371520585845815 1107 7 0.08075232657559894 1107 8 0.09634347696999868 1107 9 0.16294986677237944 1107 10-11 0.2265488121022443 1107 12-13 0.13462299561862778 1107 14-15 0.18207246799335053 1107 16-17 0.06767887751075818 1107 18-19 0.13471951818096528 1107 20-21 0.17030525976988287 1107 22-23 0.12084265051827003 1107 24-25 0.11401592426454243 1107 26-27 0.16881253594464596 1107 28-29 0.14688529294549113 1107 30-31 0.1618409772797733 1107 32-33 0.16459049972350925 1107 34-35 0.08683378535322817 1107 36-37 0.23575638902669738 1107 38-39 0.2570344386537968 1107 40-41 0.21838893944875792 1107 42-43 0.18953875160917022 1107 44-45 0.2727018653573552 1107 46-47 0.32821162597927156 1107 48-49 0.19872737871899915 1107 50-51 0.2752149191970048 1107 52-53 0.22650349227208721 1107 54-55 0.20569353292532355 1107 56-57 0.241132709581386 1107 58-59 0.2613964574490524 1107 60-61 0.34979546592682453 1107 62-63 0.39872586556960954 1107 64-65 0.4197527974143469 1107 66-67 0.4692497683296395 1107 68-69 0.36642583607723367 1107 70-71 0.3428775642249704 1107 72-73 0.3114768648885615 1107 74-75 0.3531561434032682 1107 76-77 0.271253131931843 1107 78-79 0.18092892912611092 1107 80-81 0.31704925191460376 1107 82-83 0.27116156273294933 1107 84-85 0.2844921683108552 1107 86-87 0.32648423505681734 1107 88-89 0.29851001511455166 1107 90-91 0.41033947222894085 1107 92-93 0.43515999273419226 1107 94-95 0.4297070198349431 1107 96-97 0.4817075108508959 1107 98-99 0.5087508726646988 1107 100-101 0.29444841759843854 1108 1 0.08745439601271698 1108 2 0.13744418427714322 1108 3 0.10126323964684758 1108 4 -0.10623081520867572 1108 5 -0.006756247427681217 1108 6 0.053298040874729224 1108 7 0.10388371848648603 1108 8 0.11665570846017204 1108 9 0.10637806807048378 1108 10-11 0.15242247423481103 1108 12-13 0.2122688776968218 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-0.4623208380169288 2316 76-77 -0.40890721526796625 2316 78-79 -0.3680312079989747 2316 80-81 -0.4153940760684307 2316 82-83 -0.4840222842921449 2316 84-85 -0.30560285745985993 2316 86-87 -0.3360241753144564 2316 88-89 -0.32183492787068957 2316 90-91 -0.2870413079019798 2316 92-93 -0.3070899863497374 2316 94-95 -0.2228968381438463 2316 96-97 -0.33424966146451496 2316 98-99 -0.14990669498209996 2316 100-101 -0.1478439560842908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 42.0 7 795.0 8 1780.0 9 1441.0 10 1388.0 11 1659.0 12 2046.0 13 2258.0 14 2222.0 15 2466.0 16 2720.0 17 3396.0 18 4290.0 19 5367.0 20 6963.0 21 8569.0 22 9401.0 23 9207.0 24 8558.0 25 8428.0 26 8783.0 27 9801.0 28 11042.0 29 13117.0 30 15783.0 31 19355.0 32 24898.0 33 32688.0 34 47999.0 35 76656.0 36 142949.0 37 313808.0 38 512400.0 39 202928.0 40 171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24521471267159 20.492960813634127 13.843909160378889 23.417915313315394 2 14.421852295208973 21.58543039540074 41.56577848108784 22.426938828302454 3 16.93238764819774 27.67085881109218 31.692044340209087 23.704709200500997 4 11.637523146101227 18.464418684760332 41.033104698906016 28.86495347023243 5 12.107308162869364 38.999415325853555 36.5817283390107 12.311548172266384 6 28.092074959712914 39.86573611530883 19.53339571617304 12.50879320880522 7 24.85802184807183 34.134213732055585 24.584623993812748 16.423140426059838 8 24.058813203869146 36.39530571330906 22.149515565134415 17.396365517687382 9 25.813561536623965 16.162478701627453 21.651948627863487 36.3720111338851 10-11 22.861649995314686 27.04388487594482 31.025839165776897 19.068625962963598 12-13 24.172349532194694 25.14085631665846 29.972534832985126 20.71425931816172 14-15 21.521155833477412 26.46584275564976 27.802806435902955 24.210194974969877 16-17 20.893554990385212 29.445371241686868 28.33587615994467 21.32519760798324 18-19 20.967629113340998 28.644677815509574 29.92644060146208 20.461252469687352 20-21 22.01113388510031 28.032518704953365 29.48654919511619 20.469798214830135 22-23 21.643897810045573 28.0142723842431 29.079092026126887 21.262737779584445 24-25 21.324933646743315 28.099795825980912 29.39657140745453 21.178699119821246 26-27 21.38554574646259 28.253817209480957 29.230869739087513 21.129767304968937 28-29 21.3997666582639 28.348282338221452 29.217308730386033 21.034642273128615 30-31 21.69028899796354 28.11207002363773 29.149437696568636 21.048203281830098 32-33 21.378220822054487 28.27328435092591 29.11017346212882 21.23832136489078 34-35 21.617369705432456 28.35788392832396 29.03144702231924 20.99329934392434 36-37 21.709459183013564 28.09299882405268 28.95093884414013 21.246603148793632 38-39 21.556229683233312 28.268401067987174 28.995944235548453 21.179425013231057 40-41 21.62852206781956 28.100488724235735 29.056358364337783 21.214630843606923 42-43 21.514556802479124 28.027965373564545 28.94536266294657 21.512115161009756 44-45 21.647098340079744 28.139785953830536 28.77844017384487 21.434675532244846 46-47 21.59228889012071 28.079903812397223 28.73228487790457 21.595522419577502 48-49 21.5075125192156 28.134538715119504 28.732874237686428 21.625074527978473 50-51 21.45701325217405 28.33864775296395 28.49078841262949 21.713550582232504 52-53 21.439294853943647 28.30215511154342 28.46191765201198 21.796632382500952 54-55 21.471993052540164 28.16476328615906 28.578522529751734 21.78472113154904 56-57 21.441835480877987 28.312185638660818 28.388602417620994 21.857376462840197 58-59 21.496508452698805 28.161661741589867 28.473070014399088 21.868759791312247 60-61 21.577049626032913 28.10761567771388 28.52817192323479 21.78716277301841 62-63 21.406266703797215 28.070958060518393 28.553347226493266 21.96942800919113 64-65 21.382279226118435 27.916243778763526 28.587530207064393 22.113946788053642 66-67 21.423589160167722 28.148925611763172 28.21039558551222 22.217089642556886 68-69 21.5543489593988 28.004736784450575 28.204126506063847 22.23678775008678 70-71 21.537290464268228 27.6087289342433 28.409620331350542 22.444360270137935 72-73 21.502876517612155 27.699135658919843 28.256192860640343 22.54179496282766 74-75 21.40620071348723 27.7821844640333 28.295127143530248 22.516487678949222 76-77 21.441835480877987 27.76581886715755 28.222603792859058 22.569741859105406 78-79 21.54679306890576 27.77667427314973 28.170108501267677 22.506424156676832 80-81 21.54491234507125 27.699564595934735 28.08870945390379 22.666813605090226 82-83 21.636605880792466 27.610213716217913 28.14513116893915 22.608049234050473 84-85 21.64769225286959 27.51558361170246 28.048026427799343 22.78869770762861 86-87 21.533628002064177 27.5312233151684 28.181887771599616 22.753260911167807 88-89 21.70404797759497 27.287950037416508 28.154963725126603 22.853038259861922 90-91 21.821807685759424 27.183124430008696 28.199210227970124 22.795857656261752 92-93 21.90944281741669 27.122578320599406 28.15469976388667 22.813279098097237 94-95 21.87756949769496 26.885343156210944 28.188618783217873 23.048468562876227 96-97 21.847774872737688 26.865183116511172 28.21102249345706 23.076019517294082 98-99 21.94966391135126 26.768903254246148 28.056539177787133 23.22489365661546 100-101 22.30479076452414 26.597394438600634 28.03948068265656 23.058334114218667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 90.0 1 67.0 2 51.5 3 64.0 4 73.0 5 74.5 6 114.5 7 217.5 8 368.5 9 505.0 10 495.0 11 444.5 12 527.5 13 644.5 14 694.5 15 765.5 16 835.0 17 927.5 18 1051.5 19 1170.5 20 1333.5 21 1600.0 22 1984.5 23 2424.5 24 3137.0 25 4284.5 26 6031.0 27 8760.5 28 12898.5 29 18694.0 30 24827.5 31 30734.5 32 36968.5 33 42283.0 34 46577.5 35 50147.5 36 53141.5 37 54568.5 38 54153.5 39 53659.5 40 53436.0 41 53318.5 42 53362.0 43 53528.5 44 54400.0 45 55287.5 46 55073.0 47 55151.5 48 54913.0 49 54044.5 50 52586.5 51 50433.5 52 47732.5 53 45056.5 54 42029.0 55 37949.5 56 33477.0 57 28585.0 58 24597.5 59 21030.5 60 17333.0 61 13893.0 62 10817.5 63 8241.5 64 5928.5 65 4169.0 66 2992.0 67 2276.5 68 1710.0 69 1226.0 70 916.0 71 645.0 72 445.0 73 339.5 74 268.0 75 196.0 76 134.0 77 92.5 78 75.5 79 56.5 80 46.0 81 45.5 82 37.5 83 29.0 84 23.5 85 22.5 86 20.0 87 12.5 88 11.5 89 8.0 90 5.5 91 7.5 92 5.0 93 2.0 94 2.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.3198061996576424E-4 48-49 2.309660849400874E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1515374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.543838594321805 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36865841587033 38.530015665557436 2 15.774396938141008 17.523411149097363 3 6.341433009495955 10.56682594608443 4 3.0962314101523987 6.879063107846969 5 1.7213631817353043 4.780555936425698 6 1.0584685882075693 3.5274845052336543 7 0.69156750956882 2.6888619889997343 8 0.460361330349807 2.0456188342413553 9 0.3179980632126036 1.5896549786749017 >10 1.1340269468579083 9.677166455308488 >50 0.025957524067484024 0.9824188145946756 >100 0.009179467814341567 0.7975540158338484 >500 0.0 0.0 >1k 3.576145265094444E-4 0.41136860210149995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.18688455787152214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2136 0.14095530212343618 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.599030998288212E-5 0.0 14-15 0.0 0.0 0.0 6.599030998288212E-5 0.0 16-17 0.0 0.0 0.0 6.599030998288212E-5 0.0 18-19 0.0 0.0 0.0 6.599030998288212E-5 0.0 20-21 0.0 0.0 0.0 1.3198061996576424E-4 0.0 22-23 0.0 0.0 0.0 4.949273248716158E-4 0.0 24-25 0.0 0.0 0.0 0.0013857965096405243 0.0 26-27 0.0 0.0 0.0 0.002672607554306726 0.0 28-29 0.0 0.0 0.0 0.008314779057843147 0.0 30-31 0.0 0.0 0.0 0.02778192050279337 0.0 32-33 0.0 0.0 0.0 0.07648276927016037 0.0 34-35 0.0 0.0 0.0 0.14742235250175864 0.0 36-37 0.0 0.0 0.0 0.2717151013545171 0.0 38-39 0.0 0.0 0.0 0.44896507396853846 0.0 40-41 0.0 0.0 0.0 0.6382252829994444 0.0 42-43 0.0 0.0 0.0 0.8197976209173445 0.0 44-45 0.0 0.0 0.0 1.0373346777759154 0.0 46-47 0.0 0.0 0.0 1.3014938886373923 0.0 48-49 0.0 0.0 0.0 1.5809628514148981 0.0 50-51 0.0 0.0 0.0 1.8609927318272583 0.0 52-53 0.0 0.0 0.0 2.1303981723323746 0.0 54-55 0.0 0.0 0.0 2.419105778507484 0.0 56-57 0.0 0.0 0.0 2.7524228342310213 0.0 58-59 0.0 0.0 0.0 3.1130598782874723 0.0 60-61 0.0 0.0 0.0 3.457034369073245 0.0 62-63 0.0 0.0 0.0 3.772732012031353 0.0 64-65 0.0 0.0 0.0 4.146534122929389 0.0 66-67 0.0 0.0 0.0 4.588207267644819 0.0 68-69 0.0 0.0 0.0 5.088182851230125 0.0 70-71 0.0 0.0 0.0 5.603303210956503 0.0 72-73 0.0 0.0 0.0 6.116179900143463 0.0 74-75 0.0 0.0 0.0 6.609688433350447 0.0 76-77 0.0 0.0 0.0 7.12309304501727 0.0 78-79 0.0 0.0 0.0 7.6977366643482075 0.0 80-81 0.0 0.0 0.0 8.295344911553187 0.0 82-83 0.0 0.0 0.0 8.909615712028845 0.0 84-85 0.0 0.0 0.0 9.511546324537704 0.0 86-87 0.0 0.0 0.0 10.139147167629904 0.0 88-89 0.0 0.0 0.0 10.847553145296144 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1930 0.0 49.96114 1 GTATCAA 3465 0.0 41.67388 1 CAACGCA 4150 0.0 34.45181 5 TCAACGC 4130 0.0 34.38862 4 ATCAACG 4175 0.0 34.131737 3 AACGCAG 4210 0.0 33.960808 6 TATCAAC 4415 0.0 32.599094 2 ACGCAGA 4825 0.0 29.238344 7 CGCAGAG 4870 0.0 28.578028 8 GTACATG 4100 0.0 28.268293 1 CCTACGC 90 8.948867E-4 26.388891 3 TACATGG 4295 0.0 25.54715 2 ACATGGG 4275 0.0 24.666666 3 GCAGAGT 5750 0.0 23.708696 9 CATGGGG 2365 0.0 22.093021 4 GAGTACT 3365 0.0 21.103268 12-13 AGAGTAC 5075 0.0 20.497536 10-11 CAGAGTA 5465 0.0 20.03431 10-11 AGTACTT 3965 0.0 17.79004 12-13 GTACTTT 3955 0.0 17.71492 14-15 >>END_MODULE