FastQCFastQC Report
Fri 10 Feb 2017
SRR2031499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515374
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT60530.3994393463263854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.23756511593837562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.17276263153518537No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT20050.13231057151567865RNA PCR Primer, Index 17 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.10571647659257716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.051.3578071
GTATCAA35650.045.3572161
TCAACGC43200.037.053424
AACGCAG43600.037.0403066
ATCAACG43400.036.992113
CAACGCA43400.036.8826685
TATCAAC46550.034.5909232
ACGCAGA49750.032.2705047
CGCAGAG51450.031.1119088
CGTAGAC856.409118E-427.9404373
GTACATG38650.027.6859911
ACATGGG37350.027.3419843
TACATGG38900.026.9851462
GCGTAAC908.8849216E-426.421321
GCAGAGT61950.025.0719769
GTATAGG3900.024.388911
CCTACGC1201.7114727E-423.7493723
AGAGTAC52500.021.98513410-11
CATGGGG19300.021.1652434
GAGTACT37700.020.85157212-13
Produced by FastQC (version 0.11.5)