FastQCFastQC Report
Fri 10 Feb 2017
SRR2031498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311518
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.262138987036396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1942024432756546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.11871739465260867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.059.511491
GTATCAA33100.046.2084581
TCAACGC40950.036.6544574
ATCAACG40850.036.3953483
CAACGCA41400.036.1413085
AACGCAG42300.035.5969246
TATCAAC43150.034.8957142
ACGCAGA48300.031.0766057
CGCAGAG48900.030.5010228
GCAGAGT57100.025.205789
GTACATG36950.024.6820031
TACATGG37800.023.4986782
AGAGTAC49700.022.31639910-11
GAGTACT35600.022.2155912-13
ACATGGG38500.021.590913
CAGAGTA54900.021.0245910-11
GTACTTT38300.019.90535214-15
AGTACTT38550.019.8994812-13
CCCGCTA1200.0047638519.7916689
GTATAAT10400.019.1826931
Produced by FastQC (version 0.11.5)