FastQCFastQC Report
Fri 10 Feb 2017
SRR2031498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311518
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41100.31337732307143323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.2437633337857353No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT20280.15462997839145173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.14365033495537233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.056.545421
GTATCAA37500.044.389751
TCAACGC45500.036.224364
ATCAACG45800.035.987083
CAACGCA46250.035.6369365
AACGCAG47150.034.95676
TATCAAC48550.033.9486772
GCGCGTA300.003952356531.66586724-25
ACGCAGA53750.030.8410827
CGCAGAG54450.030.4445938
GCAGAGT61700.026.4823119
GTACATG35600.025.9177741
TACATGG37000.024.519652
AGAGTAC55350.023.6421310-11
ACATGGG37200.022.8556043
GAGTACT41100.022.36257412-13
CAGAGTA60000.021.84944710-11
GTATAGA6400.021.5508291
AGTACTT46000.020.34187512-13
GTACTTT45700.019.95573214-15
Produced by FastQC (version 0.11.5)