FastQCFastQC Report
Fri 10 Feb 2017
SRR2031497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154948
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.351877313956992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32380.28035894256711125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.15429266079511805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.061.2681121
GTATCAA37550.042.1238331
TCAACGC45750.034.4699444
ATCAACG45700.034.1958433
CAACGCA47250.033.375665
AACGCAG47500.033.36
TATCAAC47700.032.662472
ACGCAGA53400.029.5318367
CGCAGAG54450.028.5261698
TGTACGC1109.4766656E-525.9090923
GTACATG37350.025.8165991
GCAGAGT60000.025.5708339
TACATGG37650.024.6015932
GAGTACT38700.024.36369512-13
ACATGGG38500.024.3051973
CAGAGTA58250.023.15879810-11
AGAGTAC54750.021.2557110-11
GTACTTT43100.021.16009314-15
CATGGGG23550.020.9766454
TATTCCG1404.851757E-420.3571435
Produced by FastQC (version 0.11.5)