##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031497_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1154948 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.160901616349825 34.0 31.0 34.0 30.0 34.0 2 32.318173632059626 34.0 31.0 34.0 30.0 34.0 3 32.41381776495565 34.0 31.0 34.0 30.0 34.0 4 35.63580611421467 37.0 35.0 37.0 35.0 37.0 5 35.62340901928052 37.0 35.0 37.0 35.0 37.0 6 35.75082601121436 37.0 37.0 37.0 35.0 37.0 7 35.779070572874275 37.0 37.0 37.0 35.0 37.0 8 35.83274744836997 37.0 37.0 37.0 35.0 37.0 9 37.659891181247986 39.0 39.0 39.0 35.0 39.0 10-11 37.66504249541971 39.0 39.0 39.0 35.0 39.0 12-13 37.6372810723946 39.0 39.0 39.0 35.0 39.0 14-15 39.14480998278711 41.0 39.5 41.0 36.0 41.0 16-17 39.157648223123466 41.0 39.5 41.0 36.0 41.0 18-19 39.1257957068197 41.0 40.0 41.0 36.0 41.0 20-21 39.09023566428965 41.0 40.0 41.0 36.0 41.0 22-23 39.02089358135604 41.0 39.0 41.0 36.0 41.0 24-25 38.96113937597191 41.0 39.0 41.0 35.5 41.0 26-27 38.852140529270585 41.0 39.0 41.0 35.0 41.0 28-29 38.74177755188978 41.0 39.0 41.0 35.0 41.0 30-31 38.55404225991127 40.5 38.5 41.0 35.0 41.0 32-33 38.41815865303027 40.0 38.0 41.0 34.5 41.0 34-35 38.26957966938771 40.0 38.0 41.0 34.0 41.0 36-37 38.11467182938107 40.0 38.0 41.0 34.0 41.0 38-39 37.95322170348795 40.0 38.0 41.0 33.0 41.0 40-41 37.798910427136114 40.0 38.0 41.0 33.0 41.0 42-43 37.63023053851775 40.0 38.0 41.0 32.5 41.0 44-45 37.754079404440716 40.0 38.0 41.0 33.0 41.0 46-47 37.573750073596386 40.0 38.0 41.0 32.0 41.0 48-49 37.40299390102412 40.0 38.0 41.0 31.5 41.0 50-51 36.328520418235286 39.0 36.0 40.0 29.5 40.5 52-53 36.37542382860527 39.0 36.5 40.0 30.0 40.5 54-55 37.11425839085396 40.0 37.0 41.0 31.0 41.0 56-57 37.125331616661526 40.0 37.0 41.0 31.0 41.0 58-59 36.922484821827474 40.0 36.0 41.0 31.0 41.0 60-61 36.74725182432456 40.0 36.0 41.0 30.0 41.0 62-63 36.49039307397389 39.0 35.0 41.0 30.0 41.0 64-65 36.214542126571935 39.0 35.0 41.0 29.0 41.0 66-67 35.908874685267214 38.5 35.0 41.0 29.0 41.0 68-69 35.597753318764134 37.5 35.0 40.5 29.0 41.0 70-71 35.23509067074882 37.0 35.0 40.0 28.5 41.0 72-73 34.86889193279697 36.5 35.0 39.0 28.0 41.0 74-75 34.501280144214284 36.0 35.0 39.0 27.5 41.0 76-77 34.133563589010066 35.5 34.5 38.5 27.0 40.5 78-79 33.725074202474914 35.0 34.0 37.0 26.0 39.5 80-81 33.37903567952843 35.0 34.0 37.0 26.0 39.0 82-83 33.06758442804352 35.0 34.0 36.5 25.0 39.0 84-85 32.764372075625914 35.0 34.0 36.0 25.0 37.0 86-87 32.48650372137966 35.0 34.0 36.0 24.0 37.0 88-89 32.248600802806706 35.0 33.5 35.0 24.0 37.0 90-91 32.03531544277318 35.0 33.0 35.0 23.0 36.0 92-93 31.857583631470852 35.0 33.0 35.0 22.5 36.0 94-95 31.70553176420064 35.0 33.0 35.0 20.0 36.0 96-97 31.552479418986827 35.0 33.0 35.0 20.0 36.0 98-99 31.390820192770583 35.0 33.0 35.0 19.0 35.0 100-101 29.823820639543946 33.5 29.0 35.0 14.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1549380603174484 1101 2 0.1441629667687394 1101 3 0.1484063130045783 1101 4 0.08676151885781991 1101 5 0.05313485227325998 1101 6 0.1516934240480836 1101 7 0.09957215440800837 1101 8 0.28093983389052113 1101 9 0.44780378820150446 1101 10-11 0.5163962743023731 1101 12-13 0.5471035123410601 1101 14-15 0.6599619265821133 1101 16-17 0.5955560383694873 1101 18-19 0.7029419945458386 1101 20-21 0.7305786579697937 1101 22-23 0.7172890454923149 1101 24-25 0.7357151427531505 1101 26-27 0.6792959304775152 1101 28-29 0.6981295842749731 1101 30-31 0.7846450368990219 1101 32-33 0.6962255574965326 1101 34-35 0.7092452539447649 1101 36-37 0.6617229099801847 1101 38-39 0.7303142455210008 1101 40-41 0.5164530959909897 1101 42-43 0.5251454769854647 1101 44-45 0.6633408422183393 1101 46-47 0.7781912193894627 1101 48-49 0.6419011753016477 1101 50-51 0.5489413758119213 1101 52-53 0.8374901848111449 1101 54-55 0.5616529255001765 1101 56-57 0.5006283042081137 1101 58-59 0.5627181465309903 1101 60-61 0.4703164698642084 1101 62-63 0.5856535578308097 1101 64-65 0.5776365014296232 1101 66-67 0.5666377713975308 1101 68-69 0.5160843496668477 1101 70-71 0.5156759604783971 1101 72-73 0.3504336176975542 1101 74-75 0.537328143339451 1101 76-77 0.5600449262866718 1101 78-79 0.6022275855484622 1101 80-81 0.5812773864591421 1101 82-83 0.5728615314165211 1101 84-85 0.5206042703765021 1101 86-87 0.43739756929454643 1101 88-89 0.4220141378320932 1101 90-91 0.42496442281691316 1101 92-93 0.6486448553383006 1101 94-95 0.6606802791924729 1101 96-97 0.475120084926143 1101 98-99 0.5401685927828979 1101 100-101 0.5051138118293075 1102 1 -0.07421096902133684 1102 2 0.051712229650071606 1102 3 0.2079085754480161 1102 4 0.10530771649320769 1102 5 -0.04135208394043133 1102 6 -0.007471661340908042 1102 7 -0.0011401503650176892 1102 8 -0.047647233690852886 1102 9 0.10500565654189131 1102 10-11 0.15540006936878825 1102 12-13 0.2131945939349862 1102 14-15 0.35821108728857354 1102 16-17 0.3239956822171024 1102 18-19 0.29441404242206204 1102 20-21 0.29061514899300533 1102 22-23 0.33684896375242346 1102 24-25 0.3510070709388131 1102 26-27 0.41498634063662365 1102 28-29 0.33996581256281644 1102 30-31 0.3816228503569121 1102 32-33 0.5496943941627137 1102 34-35 0.4536949693147818 1102 36-37 0.4328745666726377 1102 38-39 0.48074337995393535 1102 40-41 0.558355008120607 1102 42-43 0.5399980532517006 1102 44-45 0.569184514674923 1102 46-47 0.6708594430064494 1102 48-49 0.6268931472765402 1102 50-51 0.587430647451086 1102 52-53 0.6887031464225899 1102 54-55 0.6364420073860302 1102 56-57 0.5774068766792837 1102 58-59 0.7355556884956655 1102 60-61 0.7029460129965983 1102 62-63 0.7159097248137343 1102 64-65 0.7519533894751689 1102 66-67 0.7296482808122207 1102 68-69 0.6766090905805768 1102 70-71 0.7648753474292036 1102 72-73 0.7575274726092402 1102 74-75 0.7285914625629246 1102 76-77 0.5691435980134258 1102 78-79 0.7144418608367431 1102 80-81 0.6447849036099242 1102 82-83 0.680262640743635 1102 84-85 0.6096117145452382 1102 86-87 0.7370129539099324 1102 88-89 0.5786934547201383 1102 90-91 0.6099366896392766 1102 92-93 0.5234229899171972 1102 94-95 0.6221939268351484 1102 96-97 0.654620887728651 1102 98-99 0.5362895970158519 1102 100-101 0.41978028387718425 1103 1 0.15487085601637318 1103 2 0.10125974096229129 1103 3 0.2796563130045797 1103 4 0.1043556048793306 1103 5 0.048773293133471896 1103 6 0.06844745630615279 1103 7 0.1689673156983318 1103 8 0.10567101668622314 1103 9 0.17466534734128913 1103 10-11 0.23677261838839314 1103 12-13 0.2479066037389046 1103 14-15 0.2565613889477021 1103 16-17 0.2562213609501285 1103 18-19 0.3156268063737997 1103 20-21 0.33374062033538365 1103 22-23 0.3183911960299426 1103 24-25 0.341844175011218 1103 26-27 0.30827442510116754 1103 28-29 0.39228953051154036 1103 30-31 0.3238150637807422 1103 32-33 0.2116287833029844 1103 34-35 0.2187378614716451 1103 36-37 0.1638936089049139 1103 38-39 0.28089892294035934 1103 40-41 0.3402837411522768 1103 42-43 0.22137531569514124 1103 44-45 0.2780787454441551 1103 46-47 0.40113140756150045 1103 48-49 0.3758326269145513 1103 50-51 0.28203613387643145 1103 52-53 0.322943813843402 1103 54-55 0.34489553302705644 1103 56-57 0.379831933240375 1103 58-59 0.34917984007937264 1103 60-61 0.29278622792872255 1103 62-63 0.3054317836372604 1103 64-65 0.33301956594575444 1103 66-67 0.42682258322548705 1103 68-69 0.4409096184840493 1103 70-71 0.48321292284398965 1103 72-73 0.2522649349018522 1103 74-75 0.2810848637695571 1103 76-77 0.29264237252322545 1103 78-79 0.28498296189255257 1103 80-81 0.2695468757064461 1103 82-83 0.43916865507243585 1103 84-85 0.3078488940324178 1103 86-87 0.32531423596121556 1103 88-89 0.39177556256327506 1103 90-91 0.3763488314190653 1103 92-93 0.3474284574888351 1103 94-95 0.47503847811720235 1103 96-97 0.41618527309818987 1103 98-99 0.35970824332053297 1103 100-101 0.08481139247446734 1104 1 0.09840685803047222 1104 2 0.12054473592704085 1104 3 0.23319986788876435 1104 4 0.17617710538285536 1104 5 0.05106747742350137 1104 6 0.06568751169386644 1104 7 0.18712567420789838 1104 8 0.21453305092590114 1104 9 0.40342604724058617 1104 10-11 0.3887615408456 1104 12-13 0.49387997987183496 1104 14-15 0.5849412983132609 1104 16-17 0.5138654948876962 1104 18-19 0.5052809478642288 1104 20-21 0.6073606480292426 1104 22-23 0.5706444689403227 1104 24-25 0.5406860682639874 1104 26-27 0.6442512629662289 1104 28-29 0.6112881458186834 1104 30-31 0.6864141952006833 1104 32-33 0.6835698709162799 1104 34-35 0.6469966102128311 1104 36-37 0.607237642137541 1104 38-39 0.7340994516412636 1104 40-41 0.7577245770032377 1104 42-43 0.7811971938421678 1104 44-45 0.8375922147291419 1104 46-47 0.8806458209552588 1104 48-49 0.8029946359779956 1104 50-51 0.6557379843296047 1104 52-53 0.7624091335815777 1104 54-55 0.7431505053331975 1104 56-57 0.8316902539855917 1104 58-59 0.7853533672696997 1104 60-61 0.705753561765583 1104 62-63 0.7823231481891213 1104 64-65 0.68357029605653 1104 66-67 0.8291781345850069 1104 68-69 0.9136752277489038 1104 70-71 0.8969292496314978 1104 72-73 0.8088847108333752 1104 74-75 0.6976036200636173 1104 76-77 0.7172316852120488 1104 78-79 0.7461297393346484 1104 80-81 0.6732021501274019 1104 82-83 0.6769754904198706 1104 84-85 0.65893776613715 1104 86-87 0.6475889086701798 1104 88-89 0.6375317936810987 1104 90-91 0.70193417885109 1104 92-93 0.5863162973679898 1104 94-95 0.6561043769087398 1104 96-97 0.6401325918293033 1104 98-99 0.6314494945793427 1104 100-101 0.7348523038541295 1105 1 0.3435389677697458 1105 2 0.29673180261931975 1105 3 0.38462981974831223 1105 4 0.303452425688576 1105 5 0.42477883263251215 1105 6 0.33957582817512844 1105 7 0.23104583207597784 1105 8 0.3114513635070324 1105 9 0.3026337962815546 1105 10-11 0.32978803264658296 1105 12-13 0.3493727646252225 1105 14-15 0.43357246794577975 1105 16-17 0.3830698676938695 1105 18-19 0.3775457610944102 1105 20-21 0.40952638623134163 1105 22-23 0.38190275672358354 1105 24-25 0.45330852953915013 1105 26-27 0.407262864407528 1105 28-29 0.41196077328610414 1105 30-31 0.35999341156494324 1105 32-33 0.4171441975611714 1105 34-35 0.40202603584543795 1105 36-37 0.39083845476233137 1105 38-39 0.4131665048286095 1105 40-41 0.4499778644663408 1105 42-43 0.56727488216913 1105 44-45 0.35992484371003997 1105 46-47 0.5837030597772994 1105 48-49 0.7741045440629151 1105 50-51 0.7485131329130468 1105 52-53 0.7257828070996695 1105 54-55 0.5965724357245534 1105 56-57 0.6329479014484676 1105 58-59 0.6505135346843858 1105 60-61 0.5406396720520377 1105 62-63 0.7018130456796499 1105 64-65 0.7867346430170414 1105 66-67 0.6662704396802113 1105 68-69 0.6851172292740344 1105 70-71 0.5573789873911963 1105 72-73 0.6352400071561775 1105 74-75 0.5737089003784206 1105 76-77 0.4965350748353714 1105 78-79 0.5294555052451528 1105 80-81 0.631218961448262 1105 82-83 0.6687357311803339 1105 84-85 0.6985401271730751 1105 86-87 0.6878985326856863 1105 88-89 0.5626841608291713 1105 90-91 0.5667028776618395 1105 92-93 0.54195519159288 1105 94-95 0.5135530494081415 1105 96-97 0.5267012894758345 1105 98-99 0.4019499340719719 1105 100-101 0.4851666429561625 1106 1 0.3519706832234135 1106 2 0.35625502842694345 1106 3 0.3382272366615027 1106 4 0.33947381097420504 1106 5 0.20566046718310815 1106 6 0.2749478097463012 1106 7 0.26430779637694 1106 8 0.29074798809683955 1106 9 0.31107459176478613 1106 10-11 0.3134848395633938 1106 12-13 0.41604151969869463 1106 14-15 0.3963724948226641 1106 16-17 0.38072949007359824 1106 18-19 0.4320259384818712 1106 20-21 0.46442560698320534 1106 22-23 0.39445544359514173 1106 24-25 0.4084322994221523 1106 26-27 0.45196748196575953 1106 28-29 0.5167583885071352 1106 30-31 0.5837943482607884 1106 32-33 0.5095434864132571 1106 34-35 0.546954657733032 1106 36-37 0.630971640635984 1106 38-39 0.6560550256736306 1106 40-41 0.6576199805490788 1106 42-43 0.5860460272879777 1106 44-45 0.6108632647655483 1106 46-47 0.5631694809199672 1106 48-49 0.5109498512312314 1106 50-51 0.4445523332166772 1106 52-53 0.5455124401393547 1106 54-55 0.749107852379872 1106 56-57 0.7285374586880664 1106 58-59 0.798003178910669 1106 60-61 0.8004043358457835 1106 62-63 0.6758046629963559 1106 64-65 0.6719303812143664 1106 66-67 0.6868244486040638 1106 68-69 0.5518564453769201 1106 70-71 0.6381939745247891 1106 72-73 0.6630571317287419 1106 74-75 0.5701036746398032 1106 76-77 0.5852147295763288 1106 78-79 0.6041727985246581 1106 80-81 0.5740851454205611 1106 82-83 0.5172499659426819 1106 84-85 0.6619389819997465 1106 86-87 0.5650507266932223 1106 88-89 0.5249012498080177 1106 90-91 0.7802111468447706 1106 92-93 0.5681115787580815 1106 94-95 0.6700986611206581 1106 96-97 0.6519362352408216 1106 98-99 0.3954876770308644 1106 100-101 0.41341942734723247 1107 1 0.09920552149890938 1107 2 0.042023506504030195 1107 3 0.14343295598859385 1107 4 0.07579950659631862 1107 5 0.0911374879232909 1107 6 0.035104871936702864 1107 7 0.03858321267879461 1107 8 0.14788534469095538 1107 9 0.1381848115212776 1107 10-11 0.1769887225920641 1107 12-13 0.14340042404678144 1107 14-15 0.21478000943319842 1107 16-17 0.1574147446091061 1107 18-19 0.19008747096822987 1107 20-21 0.2178834193288779 1107 22-23 0.25849628780022016 1107 24-25 0.34131131533093395 1107 26-27 0.24736560331313484 1107 28-29 0.21525615277322885 1107 30-31 0.202040900074401 1107 32-33 0.22852487566532886 1107 34-35 0.37795836916850334 1107 36-37 0.419557093215154 1107 38-39 0.40023173821566616 1107 40-41 0.47297690278637816 1107 42-43 0.5101131043274663 1107 44-45 0.3290390029929924 1107 46-47 0.5012766488285223 1107 48-49 0.4455761881934137 1107 50-51 0.3297853567894009 1107 52-53 0.20232602965157298 1107 54-55 0.131591617988569 1107 56-57 0.24743572986559315 1107 58-59 0.28370193155361534 1107 60-61 0.41384251123245264 1107 62-63 0.29337028274916577 1107 64-65 0.3093794682548179 1107 66-67 0.28145642721602115 1107 68-69 0.23682806134964807 1107 70-71 0.14248338613587208 1107 72-73 0.20915418149746756 1107 74-75 0.2670399407381794 1107 76-77 0.21415195659664477 1107 78-79 0.09363823128272486 1107 80-81 0.22244337511438772 1107 82-83 0.11249884305348701 1107 84-85 0.12761280759073657 1107 86-87 0.296669327731486 1107 88-89 0.2567835184543341 1107 90-91 0.2667314542728221 1107 92-93 0.33839389561790156 1107 94-95 0.355832402036679 1107 96-97 0.3081877523758685 1107 98-99 0.2406298315384099 1107 100-101 0.397461259259547 1108 1 0.09001121852742244 1108 2 0.1340183616519468 1108 3 -0.0307271971634151 1108 4 -0.23507299812686 1108 5 0.024245773239570667 1108 6 0.15001520608510788 1108 7 0.10737911941185985 1108 8 0.12220505735819387 1108 9 0.26091921559946485 1108 10-11 0.10368243271199873 1108 12-13 0.17997308251874955 1108 14-15 0.22578663209536387 1108 16-17 0.10940162178125235 1108 18-19 0.23091609682472836 1108 20-21 0.19417524677216846 1108 22-23 0.15628907401403325 1108 24-25 0.20006698668230882 1108 26-27 0.10745656480055032 1108 28-29 0.14367934041427333 1108 30-31 0.29752886793635724 1108 32-33 0.07521189559299302 1108 34-35 0.09775670541505832 1108 36-37 0.34050446213214514 1108 38-39 0.4572997850093259 1108 40-41 0.411465217721684 1108 42-43 0.283386257339707 1108 44-45 0.25231890901621057 1108 46-47 0.18551331516538738 1108 48-49 0.2908552838553149 1108 50-51 0.2775669141593653 1108 52-53 0.16191789651360722 1108 54-55 0.3314022195876234 1108 56-57 0.3454804190935974 1108 58-59 0.4111811110784913 1108 60-61 0.33827003650520737 1108 62-63 0.2949847235134797 1108 64-65 0.4429803307556597 1108 66-67 0.4232237215986103 1108 68-69 0.4018457369632742 1108 70-71 0.32010367218085634 1108 72-73 0.2718562810556975 1108 74-75 0.34787034122314253 1108 76-77 0.40474587605992696 1108 78-79 0.3891441024761093 1108 80-81 0.45789927402652353 1108 82-83 0.43029514711487593 1108 84-85 0.42017758545593864 1108 86-87 0.40135866566943434 1108 88-89 0.4617095258700843 1108 90-91 0.3823335794296767 1108 92-93 0.35235496058344395 1108 94-95 0.3492054210880262 1108 96-97 0.31130159714770045 1108 98-99 0.42251079637092914 1108 100-101 0.21434001758499122 1109 1 0.13502454738467407 1109 2 0.08839546119542518 1109 3 0.230338036568277 1109 4 0.18824576585629416 1109 5 0.14124744661668132 1109 6 0.09959597837109868 1109 7 0.2893097386791794 1109 8 0.16897937083554382 1109 9 0.1724805360729036 1109 10-11 0.1739569092571145 1109 12-13 0.11505333909833126 1109 14-15 0.07092268508980482 1109 16-17 0.18568899209085288 1109 18-19 0.18688816843319245 1109 20-21 0.2202071933301113 1109 22-23 0.09742602811432022 1109 24-25 0.19667348392045625 1109 26-27 0.18861719501513363 1109 28-29 0.16338219634556594 1109 30-31 0.07960407640910461 1109 32-33 0.04413294650031929 1109 34-35 0.19592570840475076 1109 36-37 0.11220664665123792 1109 38-39 0.2925376614915649 1109 40-41 0.24437928653112806 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-0.49669239531643683 2316 86-87 -0.44659984338333203 2316 88-89 -0.6055771487346959 2316 90-91 -0.4847793488310401 2316 92-93 -0.5543247227612724 2316 94-95 -0.5966835734136318 2316 96-97 -0.5321565498858583 2316 98-99 -0.47837457793863436 2316 100-101 -0.4092984070080625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 27.0 7 764.0 8 1866.0 9 1444.0 10 1327.0 11 1635.0 12 1854.0 13 1915.0 14 1883.0 15 1977.0 16 2329.0 17 2943.0 18 3652.0 19 4856.0 20 6483.0 21 8228.0 22 9585.0 23 9733.0 24 8978.0 25 8301.0 26 7955.0 27 8400.0 28 9021.0 29 10715.0 30 12789.0 31 16023.0 32 20252.0 33 26881.0 34 38737.0 35 62970.0 36 119358.0 37 247630.0 38 354100.0 39 140159.0 40 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.283160800313084 19.834832390722354 14.03119447801979 22.85081233094477 2 15.226140051326986 21.77457340070722 40.496541835649744 22.50274471231605 3 17.284327952427294 27.376643796950166 31.82186557316866 23.517162677453875 4 12.312588965044313 18.592525377765927 39.38835341504552 29.706532242144235 5 12.684467179474748 38.325448418456936 35.65736292889377 13.33272147317455 6 28.604664452425567 38.72667860371203 19.088305274350013 13.580351669512394 7 24.975410148335683 34.28968230604322 23.9817723395339 16.7531352060872 8 24.412873999522056 35.48765831881609 21.913540696204503 18.185926985457353 9 26.17936045605516 15.897252516996435 21.401656178459984 36.52173084848842 10-11 23.381615449353564 26.716181161402936 30.145123416811835 19.75707997243166 12-13 24.720333729310756 25.201437640482517 29.42301298413435 20.655215646072378 14-15 22.1200867917863 26.384174871942285 27.49348888434804 24.002249451923376 16-17 21.10783342626681 29.257247945362042 28.065116351558682 21.569802276812464 18-19 21.02873895621275 28.073774750032037 30.209758361415407 20.68772793233981 20-21 22.1851979483059 27.33014819714827 29.963426924848562 20.521226929697267 22-23 22.030645535556577 27.30187852613278 29.517476111478615 21.14999982683203 24-25 21.457935768536764 27.602021909211498 29.65999335034997 21.280048971901767 26-27 21.50966969941504 27.690900369540444 29.622718944922195 21.17671098612232 28-29 21.466507583025383 27.73653012949501 29.66743957303705 21.129522714442555 30-31 21.735610607577136 27.562582904165385 29.578171484776806 21.123635003480675 32-33 21.549714792354287 27.82207510641172 29.388639142195146 21.239570959038847 34-35 21.788643298226415 27.816880067327705 29.316947602835796 21.07752903161008 36-37 21.62720745869078 27.774150870861718 29.359546923324686 21.239094747122813 38-39 21.636515237049633 27.780298333777797 29.425913547622923 21.157272881549645 40-41 21.671798210828538 27.68951502578471 29.380024035714165 21.25866272767259 42-43 21.543047825529808 27.603407252967234 29.35188424067577 21.50166068082719 44-45 21.66149471664525 27.84259551079356 29.004855629863858 21.491054142697333 46-47 21.65856961401692 27.87759957816246 28.786559036908187 21.677271770912434 48-49 21.577131378550494 27.940378113243487 28.74236435501258 21.740126153193444 50-51 21.55304827576653 28.111785119330047 28.573277757959666 21.76188884694376 52-53 21.57889359520948 28.026283434405702 28.52141395110429 21.873409019280523 54-55 21.493738246224073 28.147457721040254 28.42253504053862 21.93626899219705 56-57 21.591664732957675 28.15473077575787 28.334912047988308 21.91869244329615 58-59 21.51525436643035 28.030915677588947 28.375563228820695 22.078266727160013 60-61 21.53690036261373 27.80887104873986 28.69800198796829 21.956226600678125 62-63 21.43667940028469 27.885324707259546 28.594360958242277 22.08363493421349 64-65 21.427891125834236 27.774973418716687 28.580940440608583 22.216195014840494 66-67 21.501574096842454 27.99723450752761 28.26001690119382 22.241174494436112 68-69 21.54750690074358 27.894719069603134 28.297118138652127 22.260655891001154 70-71 21.503478944506593 27.72150780814374 28.296901678690293 22.47811156865937 72-73 21.492136442506503 27.833460900404173 28.240059292712743 22.434343364376577 74-75 21.467979510765854 28.007970921634566 28.10256392495593 22.421485642643653 76-77 21.56465052972082 27.92597588809193 28.008360549565868 22.50101303262138 78-79 21.61725030044643 27.98874927702373 27.866839026518942 22.527161396010904 80-81 21.600842635339426 28.003858182359725 27.94805480419898 22.44724437810187 82-83 21.611232713507448 27.86567014272504 27.963856381412842 22.55924076235467 84-85 21.841979032822255 27.78454094902974 27.88740272289315 22.48607729525485 86-87 21.78470372692104 27.80345954969401 27.830906672854532 22.580930050530412 88-89 21.86410124092167 27.652716832272965 27.829694497068264 22.653487429737098 90-91 21.986054783418822 27.641937126173644 27.788177476388547 22.583830614018986 92-93 22.146797951076586 27.465998469195153 27.644491353723282 22.74271222600498 94-95 22.146018695213986 27.345083934514804 27.679774327502187 22.829123042769027 96-97 22.129957366045915 27.068881023214896 27.804411973526083 22.996749637213103 98-99 22.27671722016922 27.193258917284584 27.573665654211272 22.95635820833492 100-101 22.603615054530593 27.11446749117709 27.579466781188415 22.702450673103897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 201.0 1 144.0 2 102.0 3 136.0 4 139.0 5 140.0 6 197.5 7 384.5 8 698.5 9 943.0 10 916.0 11 834.5 12 898.5 13 961.0 14 1048.5 15 1179.5 16 1317.0 17 1503.0 18 1693.0 19 1818.5 20 2071.5 21 2469.0 22 2959.0 23 3646.0 24 4514.5 25 5636.0 26 7356.0 27 9850.0 28 13048.5 29 16722.5 30 20704.5 31 24597.5 32 27715.0 33 29415.5 34 30424.5 35 31469.0 36 33002.0 37 34387.0 38 35054.5 39 35326.5 40 35482.5 41 35814.0 42 35740.5 43 36411.5 44 37471.0 45 38396.0 46 39563.0 47 40746.5 48 41640.0 49 41613.5 50 40603.5 51 39266.5 52 37953.5 53 36591.0 54 34669.5 55 31789.0 56 28536.5 57 24998.5 58 22204.5 59 19595.0 60 16443.5 61 13342.5 62 10473.5 63 8017.5 64 5795.0 65 4097.5 66 3006.0 67 2301.5 68 1743.5 69 1287.5 70 934.5 71 723.0 72 545.5 73 367.5 74 297.5 75 245.0 76 170.0 77 118.5 78 96.0 79 80.0 80 54.5 81 35.0 82 28.0 83 24.5 84 24.0 85 21.5 86 15.0 87 9.5 88 6.0 89 6.0 90 11.0 91 9.0 92 3.5 93 2.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 8.658398473351182E-5 48-49 2.5975195420053544E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1154948.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.40729707254915 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02016033165734 40.196681452269736 2 15.927258421510817 18.286817115098632 3 6.234274776906634 10.736785924493375 4 2.9856005305973756 6.855810263998556 5 1.5878127353420837 4.557601869667992 6 0.972110251803835 3.348373320754399 7 0.5974627450781798 2.400910490753863 8 0.41742468153730855 1.9170578158741192 9 0.2744354825551179 1.4179139346861027 >10 0.9606324821854993 8.318880819415218 >50 0.017053619983619355 0.6247438598883237 >100 0.005318104823394416 0.5460465928847761 >500 0.0 0.0 >1k 4.5583601876376717E-4 0.7923765402149412 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4064 0.351877313956992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3238 0.28035894256711125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1782 0.15429266079511805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7316796946702362E-4 0.0 12-13 0.0 0.0 0.0 2.5975195420053544E-4 0.0 14-15 0.0 0.0 0.0 3.4633593893404724E-4 0.0 16-17 0.0 0.0 0.0 3.8962793130080316E-4 0.0 18-19 0.0 0.0 0.0 5.195039084010709E-4 0.0 20-21 0.0 0.0 0.0 6.060878931345827E-4 0.0 22-23 0.0 0.0 0.0 0.0012987597710026773 0.0 24-25 0.0 0.0 0.0 0.0033334834122402046 0.0 26-27 0.0 0.0 0.0 0.005411499045844488 0.0 28-29 0.0 0.0 0.0 0.01861555671770504 0.0 30-31 0.0 0.0 0.0 0.04753460761869799 0.0 32-33 0.0 0.0 0.0 0.1118232162833305 0.0 34-35 0.0 0.0 0.0 0.21754226164294843 0.0 36-37 0.0 0.0 0.0 0.3857749439801619 0.0 38-39 0.0 0.0 0.0 0.6025812417528755 0.0 40-41 0.0 0.0 0.0 0.813543120556077 0.0 42-43 0.0 0.0 0.0 1.020868472000471 0.0 44-45 0.0 0.0 0.0 1.270230348032985 0.0 46-47 0.0 0.0 0.0 1.582235737020195 0.0 48-49 0.0 0.0 0.0 1.8961026816791753 0.0 50-51 0.0 0.0 0.0 2.179015851795925 0.0 52-53 0.0 0.0 0.0 2.460500386164572 0.0 54-55 0.0 0.0 0.0 2.7775276462663254 0.0 56-57 0.0 0.0 0.0 3.1373706868188007 0.0 58-59 0.0 0.0 0.0 3.517474379798917 0.0 60-61 0.0 0.0 0.0 3.846233769832062 0.0 62-63 0.0 0.0 0.0 4.159018414681873 0.0 64-65 0.0 0.0 0.0 4.5451396946009694 0.0 66-67 0.0 0.0 0.0 5.019013843047479 0.0 68-69 0.0 0.0 0.0 5.532500164509571 0.0 70-71 0.0 0.0 0.0 6.051484569002241 0.0 72-73 0.0 0.0 0.0 6.5552734841741795 0.0 74-75 0.0 0.0 0.0 7.07023173337674 0.0 76-77 0.0 0.0 0.0 7.617962020800936 0.0 78-79 0.0 0.0 0.0 8.223184074088184 0.0 80-81 0.0 0.0 0.0 8.845766216314502 0.0 82-83 0.0 0.0 0.0 9.466746554823247 0.0 84-85 0.0 0.0 0.0 10.088332981225129 0.0 86-87 0.0 0.0 0.0 10.759748490841147 0.0 88-89 0.0 0.0 0.0 11.530302662977036 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2070 0.0 61.268112 1 GTATCAA 3755 0.0 42.123833 1 TCAACGC 4575 0.0 34.469944 4 ATCAACG 4570 0.0 34.195843 3 CAACGCA 4725 0.0 33.37566 5 AACGCAG 4750 0.0 33.3 6 TATCAAC 4770 0.0 32.66247 2 ACGCAGA 5340 0.0 29.531836 7 CGCAGAG 5445 0.0 28.526169 8 TGTACGC 110 9.4766656E-5 25.909092 3 GTACATG 3735 0.0 25.816599 1 GCAGAGT 6000 0.0 25.570833 9 TACATGG 3765 0.0 24.601593 2 GAGTACT 3870 0.0 24.363695 12-13 ACATGGG 3850 0.0 24.305197 3 CAGAGTA 5825 0.0 23.158798 10-11 AGAGTAC 5475 0.0 21.25571 10-11 GTACTTT 4310 0.0 21.160093 14-15 CATGGGG 2355 0.0 20.976645 4 TATTCCG 140 4.851757E-4 20.357143 5 >>END_MODULE