Basic Statistics
Measure | Value |
---|---|
Filename | SRR2031497_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1154948 |
Sequences flagged as poor quality | 0 |
Sequence length | 101 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT | 6500 | 0.5627959007678268 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4903 | 0.4245212771484084 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4211 | 0.36460515971281826 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2344 | 0.20295286021535167 | No Hit |
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT | 1952 | 0.16901193819981505 | RNA PCR Primer, Index 17 (95% over 21bp) |
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT | 1394 | 0.12069807471851547 | RNA PCR Primer, Index 17 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 2075 | 0.0 | 64.40488 | 1 |
GTATCAA | 4120 | 0.0 | 45.480946 | 1 |
ATCAACG | 5165 | 0.0 | 35.773727 | 3 |
TCAACGC | 5190 | 0.0 | 35.601406 | 4 |
TATCAAC | 5235 | 0.0 | 35.476845 | 2 |
AACGCAG | 5330 | 0.0 | 34.7554 | 6 |
CAACGCA | 5385 | 0.0 | 34.312214 | 5 |
ACGCAGA | 5815 | 0.0 | 31.774944 | 7 |
CGCAGAG | 6000 | 0.0 | 30.716051 | 8 |
GCAGAGT | 6510 | 0.0 | 27.726019 | 9 |
TAGGTCG | 70 | 0.008392409 | 27.142311 | 5 |
GTACATG | 3285 | 0.0 | 26.493921 | 1 |
TACATGG | 3325 | 0.0 | 26.285189 | 2 |
CAGAGTA | 6210 | 0.0 | 24.476158 | 10-11 |
GAGTACT | 4610 | 0.0 | 24.367733 | 12-13 |
ACATGGG | 3425 | 0.0 | 24.269587 | 3 |
AGAGTAC | 5960 | 0.0 | 22.753319 | 10-11 |
GTACTTT | 4985 | 0.0 | 22.153517 | 14-15 |
AGTACTT | 4815 | 0.0 | 21.554605 | 12-13 |
CATGGGG | 2055 | 0.0 | 20.109085 | 4 |