FastQCFastQC Report
Fri 10 Feb 2017
SRR2031497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154948
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT65000.5627959007678268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49030.4245212771484084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.36460515971281826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23440.20295286021535167No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT19520.16901193819981505RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT13940.12069807471851547RNA PCR Primer, Index 17 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.064.404881
GTATCAA41200.045.4809461
ATCAACG51650.035.7737273
TCAACGC51900.035.6014064
TATCAAC52350.035.4768452
AACGCAG53300.034.75546
CAACGCA53850.034.3122145
ACGCAGA58150.031.7749447
CGCAGAG60000.030.7160518
GCAGAGT65100.027.7260199
TAGGTCG700.00839240927.1423115
GTACATG32850.026.4939211
TACATGG33250.026.2851892
CAGAGTA62100.024.47615810-11
GAGTACT46100.024.36773312-13
ACATGGG34250.024.2695873
AGAGTAC59600.022.75331910-11
GTACTTT49850.022.15351714-15
AGTACTT48150.021.55460512-13
CATGGGG20550.020.1090854
Produced by FastQC (version 0.11.5)