FastQCFastQC Report
Fri 10 Feb 2017
SRR2031496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423016
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.12290796449231772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.052.8158871
GTATCAA27950.045.885511
TCAACGC32700.038.0581054
ATCAACG32750.038.03
CAACGCA33350.037.316345
AACGCAG34650.035.9163066
TATCAAC37450.033.991992
ACGCAGA39500.031.506337
CGCAGAG39500.031.2658238
GTACATG45600.026.6666681
TACATGG47100.025.716562
GCAGAGT46350.025.620289
ACATGGG43600.025.6020643
TATACCG1201.7110954E-423.755
TAGACCG1050.002196476822.6190475
CGCCGTA2150.022.09302394-95
CATGGGG24100.021.8775944
AGAGTAC43500.021.6206910-11
TGCACCG1553.9072613E-521.4516125
CGTATAG1404.8525195E-420.3571411
Produced by FastQC (version 0.11.5)