FastQCFastQC Report
Fri 10 Feb 2017
SRR2031496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423016
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT26620.18706746796943954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.1680234094346093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.1311299381032961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.052.816921
GTATCAA30100.043.7734681
ATCAACG34500.037.3110123
TCAACGC34400.037.2813954
CAACGCA34750.037.042595
AACGCAG35850.036.0384946
TATCAAC39900.032.4994962
TAGCCCG600.003951565431.6661785
ACGCAGA40600.031.588187
CGCAGAG40850.031.511148
GTACATG43700.026.4498081
GCAGAGT48400.026.3993829
ACATGGG41700.025.856913
TACATGG44000.025.1530192
CCGTTTA1151.2820982E-424.7822239
TACGGTA1302.9422948E-421.9227357
CATGGGG21450.021.4798534
AGAGTAC44900.021.21069310-11
CAGAGTA48450.021.02908510-11
GAGTACT29050.020.1933212-13
Produced by FastQC (version 0.11.5)