FastQCFastQC Report
Fri 10 Feb 2017
SRR2031495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66786
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.33839427424909413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.21411673105141799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.17219177671967179No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC1130.16919713712454706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTA352.768914E-454.285716
GGTATCA1052.0008883E-1145.23811
GTATATA554.9130256E-543.181821
GTATCAA2100.042.9761921
TTATAGA459.5599587E-442.222224
AAGTGGG500.001604933438.07
TCAACGC2700.035.1851844
CAACGCA2750.034.5454565
CTATAAG550.00256232834.5454561
ATCAACG2750.034.5454563
AACGCAG2850.033.3333326
CGCAGAG2900.032.758628
TATCAAC2800.032.232142
ACGCAGA3000.031.6666687
TGATGTA600.00392457831.6666685
GCACTGA650.00580523629.230771
GCAGAGT3250.027.7692329
AGTACTT2100.027.14285912-13
CTTGTCA350.00836549427.14285532-33
GTGGTAT700.00833605527.1428551
Produced by FastQC (version 0.11.5)