##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031495_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66786 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.893480669601413 33.0 31.0 34.0 26.0 34.0 2 31.064609349264817 34.0 31.0 34.0 26.0 34.0 3 31.157847453059023 34.0 31.0 34.0 27.0 34.0 4 34.279130356661575 37.0 35.0 37.0 30.0 37.0 5 34.252717635432575 37.0 35.0 37.0 28.0 37.0 6 34.398227173359686 37.0 35.0 37.0 30.0 37.0 7 34.43061420058096 37.0 35.0 37.0 30.0 37.0 8 34.477839666996076 37.0 35.0 37.0 30.0 37.0 9 36.17918426017429 39.0 37.0 39.0 30.0 39.0 10-11 36.147351241278116 39.0 37.0 39.0 30.0 39.0 12-13 36.12269787081125 39.0 37.0 39.0 30.0 39.0 14-15 37.461324229628964 41.0 38.0 41.0 31.0 41.0 16-17 37.43765909022849 41.0 38.0 41.0 30.0 41.0 18-19 37.43468691043033 41.0 38.0 41.0 30.0 41.0 20-21 37.40158865630521 40.5 38.0 41.0 30.0 41.0 22-23 37.304075704488966 40.0 38.0 41.0 30.0 41.0 24-25 37.218795855418804 40.0 38.0 41.0 30.0 41.0 26-27 37.07830233881352 40.0 38.0 41.0 29.0 41.0 28-29 36.95063336627437 40.0 38.0 41.0 28.5 41.0 30-31 36.773710058994396 40.0 37.0 41.0 27.0 41.0 32-33 36.595207079328006 40.0 37.0 41.0 27.0 41.0 34-35 36.45543976282454 40.0 36.5 41.0 26.5 41.0 36-37 36.31332165423891 40.0 36.0 41.0 26.0 41.0 38-39 36.15436618452969 40.0 36.0 41.0 25.0 41.0 40-41 36.00156469918845 40.0 35.5 41.0 25.0 41.0 42-43 35.847894768364625 40.0 35.0 41.0 24.0 41.0 44-45 35.973969095319376 40.0 36.0 41.0 24.0 41.0 46-47 35.7740769023448 40.0 36.0 41.0 22.5 41.0 48-49 35.617846554667146 40.0 35.0 41.0 21.5 41.0 50-51 34.59510226694218 38.5 34.0 40.0 19.0 40.5 52-53 34.670701943521095 38.5 34.5 39.5 20.0 40.5 54-55 35.39921540442608 40.0 35.0 41.0 20.0 41.0 56-57 35.43374359895786 40.0 35.0 41.0 20.0 41.0 58-59 35.251751864163154 40.0 35.0 41.0 20.0 41.0 60-61 35.100215614050846 39.0 35.0 41.0 20.0 41.0 62-63 34.84138891384421 39.0 35.0 41.0 19.0 41.0 64-65 34.56213128499985 39.0 34.5 41.0 18.0 41.0 66-67 34.25872937441979 37.5 34.0 41.0 18.0 41.0 68-69 33.93766657682748 37.0 34.0 40.0 17.5 41.0 70-71 33.532244781840504 36.5 33.5 39.5 16.0 41.0 72-73 33.1315694906118 36.0 33.0 39.0 15.0 41.0 74-75 32.70066331267032 35.5 33.0 39.0 13.0 40.5 76-77 32.32135477495284 35.0 32.5 37.5 13.0 39.5 78-79 31.912017488695234 35.0 32.0 37.0 12.5 39.0 80-81 31.56323181505106 35.0 32.0 36.5 11.5 39.0 82-83 31.271202048333485 35.0 32.0 36.0 11.0 38.0 84-85 31.008362531069388 35.0 31.0 36.0 11.0 37.0 86-87 30.73001826730153 35.0 31.0 35.0 10.0 37.0 88-89 30.472913484862097 35.0 31.0 35.0 10.0 36.0 90-91 30.25766627736352 35.0 30.5 35.0 10.0 36.0 92-93 30.08050339891594 35.0 30.0 35.0 10.0 36.0 94-95 29.927447369209116 35.0 30.0 35.0 10.0 35.5 96-97 29.788847962147756 35.0 29.5 35.0 10.0 35.0 98-99 29.610464768065164 35.0 29.0 35.0 10.0 35.0 100-101 27.97995088791064 32.5 24.5 34.5 8.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.8491380070372117 1101 2 -1.3816652323951928 1101 3 -1.1020511603623468 1101 4 -1.946137908692485 1101 5 -1.8402957787475387 1101 6 -1.6873771876511512 1101 7 -2.618959400372656 1101 8 -2.137980401661622 1101 9 -2.3702435352047857 1101 10-11 -1.4822896337577518 1101 12-13 -1.1651205176836186 1101 14-15 -1.3900824227423385 1101 16-17 -1.359460732799029 1101 18-19 -1.1997333028254786 1101 20-21 -1.4854551575538508 1101 22-23 -1.2794904849063542 1101 24-25 -0.8572724914500398 1101 26-27 -1.1598378428505995 1101 28-29 -1.595876630783117 1101 30-31 -0.44445366725997815 1101 32-33 -0.7320033050364358 1101 34-35 -0.8400753636930034 1101 36-37 -1.9068318365439083 1101 38-39 -1.5224727547857668 1101 40-41 -2.0274121303203074 1101 42-43 -1.077697396346565 1101 44-45 -1.858355308306173 1101 46-47 -1.355751880627686 1101 48-49 -0.9751724962810258 1101 50-51 -1.6117070883719364 1101 52-53 -1.2148036963299091 1101 54-55 -1.7534985914162817 1101 56-57 -1.398570413642851 1101 58-59 -1.5395020998538698 1101 60-61 -1.0853579446459847 1101 62-63 -0.8977809375361332 1101 64-65 -0.6727928325596011 1101 66-67 -1.6396444285455942 1101 68-69 -1.5919035367532004 1101 70-71 -1.8872239894958156 1101 72-73 -1.886511382108953 1101 74-75 -2.0219296932068325 1101 76-77 -2.2476679833888085 1101 78-79 -2.581310250619641 1101 80-81 -1.8744279673755173 1101 82-83 -1.854639080300732 1101 84-85 -1.6793649035641245 1101 86-87 -1.749287474944751 1101 88-89 -1.8638575385899863 1101 90-91 -2.4698079187799777 1101 92-93 -2.036869778055273 1101 94-95 -2.1298780658585486 1101 96-97 -2.5026497953366267 1101 98-99 -3.1712002512859065 1101 100-101 -1.9136045788771554 1102 1 0.5508619929627869 1102 2 0.3889343807576111 1102 3 0.019032011784656078 1102 4 0.2650806599728881 1102 5 0.10709300268379707 1102 6 0.28631720306452024 1102 7 0.1423558800915572 1102 8 0.5034122482416663 1102 9 0.10906013984357088 1102 10-11 0.38985736817648586 1102 12-13 0.46079824440535333 1102 14-15 -0.15101085601119735 1102 16-17 0.13260889969613743 1102 18-19 -0.08696734537866746 1102 20-21 -0.04290196606448404 1102 22-23 0.5081304048421984 1102 24-25 -0.25843303303611265 1102 26-27 0.22971728287475202 1102 28-29 0.24396863033873473 1102 30-31 0.6665714778077145 1102 32-33 0.11983421914151648 1102 34-35 0.3216267639665702 1102 36-37 0.38291671277910666 1102 38-39 -0.17121550140085162 1102 40-41 -0.01367905488510246 1102 42-43 -0.42411905978951125 1102 44-45 0.24725397602651356 1102 46-47 0.6730682354264701 1102 48-49 0.6060654147441227 1102 50-51 1.2714650586299996 1102 52-53 1.0532814100530672 1102 54-55 0.7004665923361415 1102 56-57 0.7207719461250335 1102 58-59 1.1688151148463248 1102 60-61 0.7691875098994743 1102 62-63 0.5875188690402666 1102 64-65 1.0275940146357598 1102 66-67 0.8251524766768412 1102 68-69 0.8373034264963266 1102 70-71 0.662292451509984 1102 72-73 -0.3161051925538274 1102 74-75 -0.20587553459948538 1102 76-77 0.17941132028624196 1102 78-79 0.5341636371946699 1102 80-81 0.4913360558353155 1102 82-83 0.35019650964123983 1102 84-85 0.5558381912134358 1102 86-87 0.5476177474923887 1102 88-89 0.912931629688547 1102 90-91 0.9141379226126709 1102 92-93 0.1312153283277091 1102 94-95 0.3645126498087592 1102 96-97 0.8041200305821334 1102 98-99 0.9833452032595495 1102 100-101 0.8844611851460549 1103 1 0.5576416539797364 1103 2 0.22129185379305127 1103 3 0.567260054927953 1103 4 0.11192195427181417 1103 5 0.3064766698640682 1103 6 0.15719547733262118 1103 7 0.22525264434425907 1103 8 1.0809161003217866 1103 9 0.5626810951594408 1103 10-11 0.16520405538758354 1103 12-13 0.6423082598136745 1103 14-15 0.9103142595512139 1103 16-17 0.6675857872154012 1103 18-19 0.707947908858614 1103 20-21 0.836759050884659 1103 22-23 0.9659116066911935 1103 24-25 0.9546640393829904 1103 26-27 0.537421443121282 1103 28-29 0.9937375055313353 1103 30-31 0.42265776440248004 1103 32-33 0.19641357199205345 1103 34-35 0.25891489955979097 1103 36-37 0.9898504570009834 1103 38-39 1.4178445910490751 1103 40-41 0.8773841192289211 1103 42-43 0.9251875657882991 1103 44-45 1.6960983519895407 1103 46-47 0.9599711630073671 1103 48-49 1.0452025487965102 1103 50-51 0.7673048120968673 1103 52-53 0.2871797151378175 1103 54-55 0.8261984875595587 1103 56-57 1.0183066148461464 1103 58-59 1.0356872257862335 1103 60-61 1.5417606994218076 1103 62-63 0.8189518428461255 1103 64-65 1.4463921656372989 1103 66-67 1.056893616892559 1103 68-69 0.944699420332995 1103 70-71 0.5633710339445059 1103 72-73 1.1102430354893187 1103 74-75 1.089501969252595 1103 76-77 0.502061551411046 1103 78-79 0.4911744230190145 1103 80-81 0.38702172609725594 1103 82-83 -0.07245372148356921 1103 84-85 0.615622474726532 1103 86-87 0.5599444038868384 1103 88-89 -0.074279464302208 1103 90-91 0.4441841475741519 1103 92-93 0.11765600629381012 1103 94-95 -0.08263681090001995 1103 96-97 0.37546055446503246 1103 98-99 0.4135455114259585 1103 100-101 -0.16207194274300463 1104 1 -0.27286682059653344 1104 2 -0.16853865468152662 1104 3 -0.19545180947882557 1104 4 -0.4304509270841237 1104 5 -0.43928604200033305 1104 6 -0.2495841836843269 1104 7 -0.09678125396082748 1104 8 -0.291965255610414 1104 9 -0.013590091281237449 1104 10-11 -0.32632136834123315 1104 12-13 -0.1797256384914121 1104 14-15 -0.20832980824540215 1104 16-17 -0.5358040432930764 1104 18-19 -0.13950971826002956 1104 20-21 0.05709803393551027 1104 22-23 0.3387929626233941 1104 24-25 0.3868674292134955 1104 26-27 0.24081127362975252 1104 28-29 0.29034767502286485 1104 30-31 0.4819797983007845 1104 32-33 0.5777695041954374 1104 34-35 0.4114572724411474 1104 36-37 0.21866401632301802 1104 38-39 0.4093700147778847 1104 40-41 0.20789259380519098 1104 42-43 0.32349265053407095 1104 44-45 0.5350814028369939 1104 46-47 0.29047963758363693 1104 48-49 0.4858805148982057 1104 50-51 0.43679633752059743 1104 52-53 0.015993274459852103 1104 54-55 0.7923001824748113 1104 56-57 0.7555947504393643 1104 58-59 0.8492465478201368 1104 60-61 0.6604047672184237 1104 62-63 0.6918331987783262 1104 64-65 0.3277480978406899 1104 66-67 0.6331648033332371 1104 68-69 0.9108011152482476 1104 70-71 1.326082898351288 1104 72-73 1.3221074422689725 1104 74-75 0.5810273929814116 1104 76-77 0.13765477175003582 1104 78-79 0.33015747386647476 1104 80-81 0.3615979972836989 1104 82-83 0.46144458360117824 1104 84-85 0.4885038306587397 1104 86-87 0.5090969462597243 1104 88-89 0.2647035865452487 1104 90-91 -0.2168328015783878 1104 92-93 -0.11963212929941491 1104 94-95 -0.24365376005256323 1104 96-97 1.112748690058254 1104 98-99 0.4898166978666332 1104 100-101 -0.5095295698616518 1105 1 0.9956895791696851 1105 2 1.0713795218234416 1105 3 0.8726370274586337 1105 4 0.652541475019909 1105 5 0.6428296797997817 1105 6 0.7869441623121674 1105 7 0.6150831528188334 1105 8 0.5560768250441726 1105 9 0.9551416445457619 1105 10-11 1.145186521781504 1105 12-13 0.9570364889194636 1105 14-15 0.684569081292878 1105 16-17 0.7993800595707441 1105 18-19 1.0475154132420101 1105 20-21 1.1605463097975743 1105 22-23 0.971610028666646 1105 24-25 1.1045763713526 1105 26-27 1.0485260603042121 1105 28-29 1.2168526428779245 1105 30-31 1.1679821361149223 1105 32-33 1.6330003633421413 1105 34-35 1.7371440053458826 1105 36-37 0.744672198672518 1105 38-39 1.4786277587872334 1105 40-41 1.495882073641539 1105 42-43 1.7324640123108068 1105 44-45 1.6737430042396824 1105 46-47 1.301438141382583 1105 48-49 1.5919588316720237 1105 50-51 1.6368255601973942 1105 52-53 2.4084538238461732 1105 54-55 2.7532879089505613 1105 56-57 2.536132447692431 1105 58-59 2.360351165003067 1105 60-61 2.0698144691471185 1105 62-63 2.3977069568145666 1105 64-65 2.049694328115379 1105 66-67 2.2605756741690115 1105 68-69 2.115359852828611 1105 70-71 2.3669946458673508 1105 72-73 2.2182208262549494 1105 74-75 2.2500805782531828 1105 76-77 2.791950505239221 1105 78-79 3.0681761763796196 1105 80-81 3.1035617611644675 1105 82-83 2.904898703998608 1105 84-85 2.7652425799281737 1105 86-87 2.444953170689878 1105 88-89 2.657602476083529 1105 90-91 2.4392162925186334 1105 92-93 1.974318776603564 1105 94-95 1.6483684491818025 1105 96-97 1.6969099992341725 1105 98-99 1.8632825073347838 1105 100-101 2.4308561694720723 1106 1 -0.563423721322927 1106 2 -0.036173844350614104 1106 3 -0.31920752645488193 1106 4 -0.13289913800690556 1106 5 -0.5353312397404508 1106 6 -0.6698155530682399 1106 7 -0.607139069403388 1106 8 -0.8434852986558852 1106 9 -0.6762212454378158 1106 10-11 -0.48330280498368694 1106 12-13 -0.5643099807028662 1106 14-15 -0.385354290354627 1106 16-17 0.05526402235125971 1106 18-19 -0.725062583473914 1106 20-21 -0.2921083152708377 1106 22-23 0.1354031321149236 1106 24-25 -0.3354893100923988 1106 26-27 -0.43204317888571353 1106 28-29 -0.6215436351735306 1106 30-31 -0.3617113504751117 1106 32-33 -0.4827631834558872 1106 34-35 -0.9545637122239015 1106 36-37 -0.30568357582117756 1106 38-39 -0.3218648520502043 1106 40-41 -0.043116284322337606 1106 42-43 -0.15875109442153956 1106 44-45 -0.5231644943919491 1106 46-47 -0.6228913605331243 1106 48-49 -0.9206301119514038 1106 50-51 -1.2680731809082388 1106 52-53 -0.8022741455024871 1106 54-55 -1.1275276356580903 1106 56-57 -0.6584488330957399 1106 58-59 -0.5249799789487639 1106 60-61 -0.3948817541697949 1106 62-63 -0.9075749260535275 1106 64-65 -1.1403713533296127 1106 66-67 -0.7725387210143495 1106 68-69 -0.7155104263175289 1106 70-71 -0.6261635369459952 1106 72-73 -1.3111989876476215 1106 74-75 -0.27961290833685837 1106 76-77 -0.33530729443237206 1106 78-79 -1.1971494941184595 1106 80-81 -1.0135701490708904 1106 82-83 -0.5922277327830052 1106 84-85 -0.41154997617472944 1106 86-87 -1.7308815310068937 1106 88-89 -1.5631145463576281 1106 90-91 -0.9422834338086865 1106 92-93 -1.1560862589738825 1106 94-95 -1.290465705169595 1106 96-97 -1.2447977183356116 1106 98-99 -1.0045335846192387 1106 100-101 -0.9169818162969499 1107 1 0.13523699296278835 1107 2 0.9787071080303384 1107 3 0.19914564814829205 1107 4 0.5841147508819802 1107 5 1.2980020935928849 1107 6 0.8505217485190641 1107 7 0.8807081528188334 1107 8 1.3653440664234822 1107 9 1.2999692307526587 1107 10-11 0.9668460045401233 1107 12-13 0.8880709716780828 1107 14-15 1.3082220985342587 1107 16-17 1.289142990605228 1107 18-19 1.1145951546213269 1107 20-21 1.2367855339355103 1107 22-23 1.2994656321149236 1107 24-25 1.3769362851457032 1107 26-27 1.604291146511109 1107 28-29 1.451497039429647 1107 30-31 1.4750942050804454 1107 32-33 1.741567173686967 1107 34-35 1.638814263966573 1107 36-37 1.8976894400518347 1107 38-39 1.884608362235511 1107 40-41 1.5123152632967134 1107 42-43 1.4937786674832196 1107 44-45 1.1723960214810631 1107 46-47 1.6861364172446542 1107 48-49 1.8020881420168493 1107 50-51 2.147601422266362 1107 52-53 1.50328141005307 1107 54-55 1.9925120468815933 1107 56-57 1.578158309761399 1107 58-59 1.8067412512099637 1107 60-61 1.7115170553540153 1107 62-63 2.0402177326766306 1107 64-65 1.8923667419084822 1107 66-67 1.7745842948586628 1107 68-69 2.0073318355872303 1107 70-71 2.5235140424190803 1107 72-73 2.0506561710825366 1107 74-75 2.5493801472186988 1107 76-77 2.15725222937715 1107 78-79 2.368822728103762 1107 80-81 1.5698871921989515 1107 82-83 1.7801681005503305 1107 84-85 1.3673438730316185 1107 86-87 0.9872484293105686 1107 88-89 1.7812554933249096 1107 90-91 1.779194740794491 1107 92-93 2.0249653283277063 1107 94-95 1.4253081043542153 1107 96-97 2.059517757854863 1107 98-99 1.6022372487140935 1107 100-101 1.4402850487824175 1108 1 0.878134720235515 1108 2 0.7798434716667018 1108 3 0.5675168602695031 1108 4 0.7862927811850113 1108 5 1.1616384572292517 1108 6 0.9348020515493687 1108 7 1.0090225467582243 1108 8 0.7640183088477244 1108 9 0.48178741257083857 1108 10-11 0.7125846409037564 1108 12-13 1.100192183799301 1108 14-15 0.9429285379281964 1108 16-17 1.0841240512112904 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74-75 0.4611278324038892 2316 76-77 0.2934405009820864 2316 78-79 0.6361838392148762 2316 80-81 0.6301688280014197 2316 82-83 0.5806117733898368 2316 84-85 0.2648168668587765 2316 86-87 0.9640038305451348 2316 88-89 1.1679260180162707 2316 90-91 1.3557765309179466 2316 92-93 1.542326439438817 2316 94-95 0.8770827957122407 2316 96-97 0.7067013998301732 2316 98-99 1.0818861684671788 2316 100-101 1.534517301868835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 17.0 7 261.0 8 576.0 9 433.0 10 378.0 11 418.0 12 435.0 13 450.0 14 368.0 15 342.0 16 374.0 17 341.0 18 392.0 19 397.0 20 534.0 21 618.0 22 640.0 23 653.0 24 615.0 25 572.0 26 604.0 27 650.0 28 685.0 29 785.0 30 861.0 31 1093.0 32 1387.0 33 1685.0 34 2294.0 35 3643.0 36 6512.0 37 13315.0 38 18869.0 39 5582.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.519390291378436 19.91285598778187 13.663043152756565 23.904710568083132 2 14.5210073967598 22.19177671967179 40.631270026652295 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46-47 21.682687988500586 27.613571706645107 28.719342377144912 21.984397927709402 48-49 21.789746354026292 27.816458539214807 28.459557392267843 21.93423771449106 50-51 21.52921270925044 27.8763513311173 28.41089449884706 22.183541460785193 52-53 21.535950648339472 28.079238163687 28.094960021561405 22.289851166412124 54-55 21.683436648399365 27.973677117958857 28.312071392207947 22.03081484143383 56-57 21.672206749917645 28.005120833707664 28.005120833707664 22.317551582667026 58-59 21.550923846315094 27.925762884436857 28.164585392148055 22.35872787709999 60-61 21.472314556943072 27.909292366663674 28.288862935345733 22.329530141047524 62-63 21.303117419818523 28.100200640852872 28.03881052915282 22.557871410175785 64-65 21.443865480789388 27.906297727068548 27.861378133141677 22.788458659000387 66-67 21.569640343784627 27.753571107717185 27.818704518911147 22.85808402958704 68-69 21.59210014074806 27.915281645853923 27.621806965531697 22.870811247866317 70-71 21.492528374210163 27.264696193813077 28.03431856976013 23.208456862216632 72-73 21.507501572185788 27.376246518731474 27.983409696643008 23.132842212439733 74-75 21.383224028988113 27.486299523852303 27.708651513790315 23.42182493336927 76-77 21.4154164046357 27.572395412212142 27.791004102656245 23.221184080495913 78-79 21.314347318300243 27.296888569460663 27.912287006258797 23.476477105980294 80-81 21.42140568382595 27.25047165573623 27.95645794028689 23.37166472015093 82-83 21.54867786661875 27.138921330817833 27.94148474231126 23.37091606025215 84-85 21.689425927589614 26.954002335818885 27.94073608241248 23.41583565417902 86-87 21.467822597550384 27.106728955170244 27.967687838768608 23.457760608510767 88-89 21.81295481088851 26.79977839666996 27.791752762555028 23.595514029886502 90-91 21.94621627287156 26.712933848411343 27.95945257988201 23.381397298835086 92-93 22.197017338963256 26.542988051388015 27.828437097595305 23.431557512053423 94-95 22.277123948132843 26.481597939687955 27.59036324978289 23.65091486239631 96-97 22.22471775521816 26.289192345701196 27.750576468122063 23.735513430958584 98-99 22.339262719731682 26.191117898960858 27.67945377773785 23.79016560356961 100-101 22.59904770460875 26.142455005540082 27.594855209175577 23.66364208067559 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 2.0 2 3.5 3 6.5 4 5.0 5 4.0 6 6.0 7 14.5 8 33.5 9 43.0 10 44.0 11 41.0 12 40.5 13 46.5 14 46.5 15 58.5 16 65.5 17 65.5 18 80.0 19 85.0 20 95.5 21 119.5 22 147.5 23 182.0 24 199.5 25 227.5 26 307.0 27 403.0 28 499.5 29 624.5 30 778.0 31 903.0 32 1052.0 33 1268.0 34 1465.5 35 1604.0 36 1801.0 37 2009.0 38 2090.0 39 2173.5 40 2292.5 41 2398.0 42 2465.0 43 2525.0 44 2657.5 45 2746.5 46 2847.0 47 2907.0 48 2877.5 49 2833.0 50 2698.5 51 2571.0 52 2375.0 53 2201.0 54 1986.5 55 1712.5 56 1489.0 57 1250.0 58 1081.5 59 910.5 60 732.5 61 585.0 62 448.0 63 339.0 64 254.5 65 184.0 66 143.5 67 119.5 68 98.0 69 75.5 70 62.0 71 55.5 72 46.5 73 36.5 74 26.0 75 20.0 76 14.5 77 15.0 78 11.0 79 10.5 80 12.0 81 6.0 82 2.0 83 0.5 84 2.5 85 4.5 86 3.5 87 2.5 88 2.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 66786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.46120444404515 #Duplication Level Percentage of deduplicated Percentage of total 1 95.92271583972828 89.65052555924893 2 3.249010717890386 6.073129098912946 3 0.47261250580752656 1.3251280208426914 4 0.13617648472420255 0.5090887311712036 5 0.07689966196190262 0.3593567514149672 6 0.03844983098095131 0.21561405084898033 7 0.02563322065396754 0.1676998173269847 8 0.020826991781348628 0.15572125894648578 9 0.014418686617856743 0.12128290360255142 >10 0.036847754690078345 0.5285538885395142 >50 0.0 0.0 >100 0.006408305163491885 0.8938999191447309 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 0.33839427424909413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 143 0.21411673105141799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.17219177671967179 No Hit ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC 113 0.16919713712454706 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0014973197975623634 0.0 24-25 0.0 0.0 0.0 0.0029946395951247267 0.0 26-27 0.0 0.0 0.0 0.006737939089030635 0.0 28-29 0.0 0.0 0.0 0.02994639595124727 0.0 30-31 0.0 0.0 0.0 0.11379630461473961 0.0 32-33 0.0 0.0 0.0 0.22834126912826042 0.0 34-35 0.0 0.0 0.0 0.4132602641272123 0.0 36-37 0.0 0.0 0.0 0.6820291677896565 0.0 38-39 0.0 0.0 0.0 1.0773215943461203 0.0 40-41 0.0 0.0 0.0 1.5272661935136107 0.0 42-43 0.0 0.0 0.0 1.9839487317701314 0.0 44-45 0.0 0.0 0.0 2.457850447698619 0.0 46-47 0.0 0.0 0.0 2.9572066001856676 0.0 48-49 0.0 0.0 0.0 3.477525229838589 0.0 50-51 0.0 0.0 0.0 3.9446890066780465 0.0 52-53 0.0 0.0 0.0 4.44105051956997 0.0 54-55 0.0 0.0 0.0 4.924684814182613 0.0 56-57 0.0 0.0 0.0 5.422543646872099 0.0 58-59 0.0 0.0 0.0 5.919653819662804 0.0 60-61 0.0 0.0 0.0 6.383822956907137 0.0 62-63 0.0 0.0 0.0 6.794837241338005 0.0 64-65 0.0 0.0 0.0 7.284460815140898 0.0 66-67 0.0 0.0 0.0 7.869164196089001 0.0 68-69 0.0 0.0 0.0 8.46360015572126 0.0 70-71 0.0 0.0 0.0 9.037822298086425 0.0 72-73 0.0 0.0 0.0 9.570119486119845 0.0 74-75 0.0 0.0 0.0 10.094930075165454 0.0 76-77 0.0 0.0 0.0 10.619740664211061 0.0 78-79 0.0 0.0 0.0 11.166262390321325 0.0 80-81 0.0 0.0 0.0 11.749468451471866 0.0 82-83 0.0 0.0 0.0 12.343155751205343 0.0 84-85 0.0 0.0 0.0 12.920372533165633 0.0 86-87 0.0 0.0 0.0 13.496840655227142 0.0 88-89 0.0 0.0 0.0 14.095019914353308 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCTTA 35 2.768914E-4 54.28571 6 GGTATCA 105 2.0008883E-11 45.2381 1 GTATATA 55 4.9130256E-5 43.18182 1 GTATCAA 210 0.0 42.976192 1 TTATAGA 45 9.5599587E-4 42.22222 4 AAGTGGG 50 0.0016049334 38.0 7 TCAACGC 270 0.0 35.185184 4 CAACGCA 275 0.0 34.545456 5 CTATAAG 55 0.002562328 34.545456 1 ATCAACG 275 0.0 34.545456 3 AACGCAG 285 0.0 33.333332 6 CGCAGAG 290 0.0 32.75862 8 TATCAAC 280 0.0 32.23214 2 ACGCAGA 300 0.0 31.666668 7 TGATGTA 60 0.003924578 31.666668 5 GCACTGA 65 0.005805236 29.23077 1 GCAGAGT 325 0.0 27.769232 9 AGTACTT 210 0.0 27.142859 12-13 CTTGTCA 35 0.008365494 27.142855 32-33 GTGGTAT 70 0.008336055 27.142855 1 >>END_MODULE