FastQCFastQC Report
Fri 10 Feb 2017
SRR2031495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66786
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17782.662234600065882No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT6761.0121881831521577RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT5020.7516545383763065RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG2800.4192495433174618RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.41026562453208754No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT2040.3054532387027221RNA PCR Primer, Index 17 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.23657652801485343No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG1160.17368909651723413TruSeq Adapter, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.17069445692210944No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC740.1108016650196149TruSeq Adapter, Index 25 (95% over 24bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA720.10780702542449017RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.058.8531461
GTATCAA2100.047.5352331
AACGCAG2400.041.5621726
ATCAACG2450.040.7139633
TATCAAC2500.039.8996852
GTATAAA608.1712205E-539.6126941
CAACGCA2550.039.117345
TCAACGC2550.039.117344
GACAGCC500.001604995237.9997027
CGCAGAG2750.036.2724388
ACGCAGA2800.035.624727
GCAGAGT2850.034.9997259
CAGTACT550.002562427234.5451854
CAGAGTA2600.028.31708310-11
GAGTACT1750.027.14264512-13
GTACTTT2000.023.74981314-15
AGAGTAC2650.023.301710-11
ACTTTTT2250.021.11094516-17
GTACATG1401.3202325E-520.3722421
ACATGGG1656.2097286E-520.1513563
Produced by FastQC (version 0.11.5)