##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031495_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66786 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.744003234210766 34.0 31.0 34.0 31.0 34.0 2 32.91400892402599 34.0 33.0 34.0 31.0 34.0 3 33.01840206031204 34.0 33.0 34.0 31.0 34.0 4 36.37900158715899 37.0 37.0 37.0 35.0 37.0 5 36.27968436498667 37.0 37.0 37.0 35.0 37.0 6 36.28595813493846 37.0 37.0 37.0 35.0 37.0 7 36.33736112358878 37.0 37.0 37.0 35.0 37.0 8 36.335654179019556 37.0 37.0 37.0 35.0 37.0 9 38.20095529003085 39.0 39.0 39.0 37.0 39.0 10-11 38.17005809600815 39.0 39.0 39.0 37.0 39.0 12-13 38.17467732758362 39.0 39.0 39.0 37.0 39.0 14-15 39.72614769562483 41.0 40.0 41.0 37.0 41.0 16-17 39.70266223460007 41.0 40.0 41.0 37.0 41.0 18-19 39.719469649327706 41.0 40.0 41.0 37.0 41.0 20-21 39.71086006049172 41.0 40.0 41.0 37.0 41.0 22-23 39.699173479471746 41.0 40.0 41.0 37.0 41.0 24-25 39.6367951367053 41.0 40.0 41.0 37.0 41.0 26-27 39.50113796304615 41.0 39.5 41.0 36.5 41.0 28-29 39.40525708980924 41.0 39.0 41.0 36.0 41.0 30-31 39.261768933608835 41.0 39.0 41.0 36.0 41.0 32-33 38.300040427634535 40.0 38.0 41.0 34.0 41.0 34-35 38.505270565687425 40.0 38.0 41.0 35.0 41.0 36-37 38.76170904081694 40.0 38.0 41.0 35.0 41.0 38-39 38.605201688976734 40.0 38.0 41.0 35.0 41.0 40-41 38.544867187733956 40.0 38.0 41.0 35.0 41.0 42-43 38.36816099182463 40.0 38.0 41.0 34.5 41.0 44-45 38.21551672506214 40.0 38.0 41.0 34.0 41.0 46-47 38.008549696044085 40.0 38.0 41.0 33.0 41.0 48-49 37.9058335579313 40.0 38.0 41.0 33.0 41.0 50-51 37.855456233342316 40.0 38.0 41.0 33.0 41.0 52-53 37.979217201209835 40.0 38.0 41.0 33.5 41.0 54-55 37.90948701823736 40.0 38.0 41.0 33.0 41.0 56-57 37.70066331267032 40.0 37.5 41.0 33.0 41.0 58-59 37.42600994220346 40.0 37.0 41.0 32.0 41.0 60-61 37.07169915850628 40.0 36.5 41.0 31.0 41.0 62-63 36.67169766118648 39.5 36.0 41.0 30.5 41.0 64-65 36.26967478213997 39.0 35.0 41.0 29.0 41.0 66-67 35.71896056059653 39.0 35.0 41.0 27.0 41.0 68-69 35.11115353517204 38.0 35.0 40.5 24.0 41.0 70-71 34.520685473003326 37.0 35.0 40.0 21.0 41.0 72-73 34.01454646183332 37.0 35.0 39.0 19.5 41.0 74-75 33.57701464378762 36.0 34.0 39.0 19.0 41.0 76-77 32.2221124187704 35.0 32.0 37.0 18.5 39.0 78-79 32.839180367142816 35.0 34.0 37.0 19.0 39.0 80-81 32.65191058006169 35.0 34.0 37.0 19.0 39.0 82-83 32.375078609289375 35.0 34.0 36.0 18.5 38.5 84-85 32.11512891923457 35.0 34.0 36.0 18.0 37.0 86-87 31.882318150510585 35.0 34.0 36.0 18.0 37.0 88-89 31.671967178750037 35.0 33.5 35.0 18.0 36.5 90-91 31.51355074416794 35.0 33.0 35.0 17.5 36.0 92-93 31.372593058425416 35.0 33.0 35.0 16.5 36.0 94-95 31.268986015033093 35.0 33.0 35.0 16.0 36.0 96-97 31.175665558650017 35.0 33.0 35.0 15.0 35.5 98-99 31.125670050609408 35.0 33.0 35.0 15.0 35.0 100-101 30.0242640673195 34.0 30.0 35.0 12.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.28001170399015507 1101 2 -0.37420190692890287 1101 3 -0.5193813041265187 1101 4 -0.5056943365100395 1101 5 -0.47544793887265513 1101 6 -0.6596431250082233 1101 7 -0.5529029832910552 1101 8 -0.6315653100066783 1101 9 -0.884993004678023 1101 10-11 0.12580905498678874 1101 12-13 0.25895519144911816 1101 14-15 0.3273795215833246 1101 16-17 0.26813632744482163 1101 18-19 0.31834945743023724 1101 20-21 0.24166322200154156 1101 22-23 0.4652845235300731 1101 24-25 0.2725434527694688 1101 26-27 0.3052349790587172 1101 28-29 0.10726329988747807 1101 30-31 0.09079681830656483 1101 32-33 0.7349332507006281 1101 34-35 0.3124281882974529 1101 36-37 0.041176404816162915 1101 38-39 0.2670070670339584 1101 40-41 0.3298032601894718 1101 42-43 -0.6970039138822912 1101 44-45 0.0906250645469413 1101 46-47 0.6881376777620929 1101 48-49 1.129210755988332 1101 50-51 -0.24484614924630677 1101 52-53 -0.13356983003709644 1101 54-55 -0.7088301466007465 1101 56-57 -0.83485206698154 1101 58-59 -1.240123800971297 1101 60-61 -0.5274046511849804 1101 62-63 -1.280943745468882 1101 64-65 -0.8835450445039044 1101 66-67 -1.0916552835977456 1101 68-69 -1.9843091664549277 1101 70-71 -1.983415219684609 1101 72-73 -1.6215782625208988 1101 74-75 -2.7142868912468074 1101 76-77 -2.465155422676588 1101 78-79 -2.831219636701512 1101 80-81 -1.8714900733745665 1101 82-83 -1.853998271689946 1101 84-85 -1.1118393942405476 1101 86-87 -1.8546356882844321 1101 88-89 -1.7523435240863456 1101 90-91 -1.5051197237965113 1101 92-93 -1.5318463001605664 1101 94-95 -1.6038536089818685 1101 96-97 -1.8916816388087199 1101 98-99 -2.358343191014015 1101 100-101 -1.754154788805188 1102 1 0.5064486441723233 1102 2 0.5998793309821195 1102 3 0.42143107498373666 1102 4 0.2734159149406352 1102 5 0.35240505919310294 1102 6 0.5415174165778538 1102 7 0.44168115597393154 1102 8 0.4601174752931314 1102 9 0.5200360088616236 1102 10-11 0.47029648245294453 1102 12-13 0.43632463052068005 1102 14-15 0.8482692701326471 1102 16-17 0.6224883583926015 1102 18-19 0.6104190899254007 1102 20-21 0.6673885604928387 1102 22-23 0.5556133436461295 1102 24-25 0.7787330078951982 1102 26-27 0.9359893310896652 1102 28-29 0.775154982672781 1102 30-31 0.6668122921943791 1102 32-33 1.0591112004104914 1102 34-35 0.8673604900382585 1102 36-37 0.6082943932107483 1102 38-39 1.0776453649062958 1102 40-41 0.6222597398799934 1102 42-43 0.9612165890190596 1102 44-45 1.1801801902722815 1102 46-47 1.0446173682843423 1102 48-49 1.2299844503790425 1102 50-51 1.2574749339258418 1102 52-53 1.372619725088633 1102 54-55 1.2023884220646366 1102 56-57 0.9423239484923513 1102 58-59 1.245369429202782 1102 60-61 0.8176630470742055 1102 62-63 0.9600620572390497 1102 64-65 0.7060100812214287 1102 66-67 0.9122131883558282 1102 68-69 1.174104760044095 1102 70-71 0.998016114938217 1102 72-73 1.1163327626241681 1102 74-75 0.8919026638789163 1102 76-77 0.7155022175555175 1102 78-79 0.9351246573023602 1102 80-81 1.1501733695654721 1102 82-83 1.369986254422244 1102 84-85 1.1742341067265727 1102 86-87 1.9391747565898427 1102 88-89 1.535664212857565 1102 90-91 1.3312439125671247 1102 92-93 1.2685405470347888 1102 94-95 1.3425680544610685 1102 96-97 1.4589953437831547 1102 98-99 0.8832428824869503 1102 100-101 1.0479728707692786 1103 1 0.3027506472539869 1103 2 0.3946405020144823 1103 3 0.2537885480191733 1103 4 0.02441745577268506 1103 5 -0.09751789920442633 1103 6 -0.15802033380735736 1103 7 0.1344392453421932 1103 8 0.0305334999464435 1103 9 0.3169543447630119 1103 10-11 0.1541177459352241 1103 12-13 0.07900567828647809 1103 14-15 0.246420271673486 1103 16-17 9.167097023095039E-4 1103 18-19 0.3114976723599199 1103 20-21 -0.20117846570130382 1103 22-23 -0.15840822800255694 1103 24-25 0.02696105103849078 1103 26-27 -0.33396444394885805 1103 28-29 0.4267728563245541 1103 30-31 0.16742862501410372 1103 32-33 0.29840241766780906 1103 34-35 -0.3480478304548029 1103 36-37 0.24943460892723124 1103 38-39 0.6505267208385064 1103 40-41 0.5099330834855422 1103 42-43 0.5411857723780784 1103 44-45 0.6353419776374594 1103 46-47 0.3518592789160877 1103 48-49 0.6133434642465332 1103 50-51 0.7753485856977989 1103 52-53 0.9937291241641262 1103 54-55 0.2051619197533867 1103 56-57 0.48916524279126605 1103 58-59 0.2692523259670381 1103 60-61 0.243240859092694 1103 62-63 0.8018186057752601 1103 64-65 0.64422271604424 1103 66-67 0.3459574102356413 1103 68-69 0.11432047653099886 1103 70-71 0.8380777482201935 1103 72-73 0.7449922387412684 1103 74-75 0.4977578256662838 1103 76-77 0.6942387352750856 1103 78-79 0.9946007744055905 1103 80-81 0.7435477917534499 1103 82-83 0.9761495826194704 1103 84-85 0.6652972808405977 1103 86-87 0.6621331541245112 1103 88-89 0.6291927182504757 1103 90-91 0.5965751914577311 1103 92-93 0.7229319183753091 1103 94-95 0.7237699034595266 1103 96-97 1.1961293961714468 1103 98-99 1.5430271660000479 1103 100-101 0.75305761653199 1104 1 0.3027506472539869 1104 2 -0.04603746408721321 1104 3 0.270737700561547 1104 4 0.05831576085743251 1104 5 0.08892277876167043 1104 6 0.14706441195535547 1104 7 0.18528670296930727 1104 8 0.1830758728277999 1104 9 0.3847509549325068 1104 10-11 0.19649062729115485 1104 12-13 0.20612432235427747 1104 14-15 -0.16035938934346916 1104 16-17 -0.29569345978922 1104 18-19 -0.26477351408075833 1104 20-21 0.0276350936207308 1104 22-23 0.1636256703025225 1104 24-25 0.3066220679876466 1104 26-27 0.08128979333928044 1104 28-29 0.24880675462963353 1104 30-31 0.39624218433613834 1104 32-33 0.4509447905491655 1104 34-35 0.4570369153079099 1104 36-37 0.09689223604587482 1104 38-39 -0.23930378763607507 1104 40-41 0.12010257501096078 1104 42-43 0.5920332300051925 1104 44-45 0.2878843505188158 1104 46-47 -0.6905136024398502 1104 48-49 -0.7256395866009271 1104 50-51 -0.8941429397259313 1104 52-53 -0.44694884193756224 1104 54-55 -0.5744990971957691 1104 56-57 -0.6040550961917788 1104 58-59 -0.5612561486092389 1104 60-61 -1.0364201578564547 1104 62-63 -0.6558085128688091 1104 64-65 -1.3557772839557671 1104 66-67 -1.6201442846796112 1104 68-69 -0.9619507099096793 1104 70-71 -1.3483629297459103 1104 72-73 -1.9922958968519495 1104 74-75 -1.5276659031472732 1104 76-77 -1.1362697393011842 1104 78-79 -1.615568717119828 1104 80-81 -0.8750962760431591 1104 82-83 -1.5407995699229033 1104 84-85 -0.9957196683119491 1104 86-87 -1.227697354350063 1104 88-89 -1.7013157563257977 1104 90-91 -1.7000349780337984 1104 92-93 -1.6584240138280748 1104 94-95 -0.9880945033201343 1104 96-97 -1.2614977224726225 1104 98-99 -1.52476944416944 1104 100-101 -0.3232135699086882 1105 1 0.3036273275578978 1105 2 0.15881350026111818 1105 3 -0.2926755080883652 1105 4 0.031723124971982486 1105 5 0.16431728489843778 1105 6 0.13712870184431125 1105 7 -0.08151633618907539 1105 8 0.1927193561708762 1105 9 0.3090642220277715 1105 10-11 0.29803942916140613 1105 12-13 0.5236287057557973 1105 14-15 0.4537551635495731 1105 16-17 0.17844447124525686 1105 18-19 0.3392592153172558 1105 20-21 0.3430938896464397 1105 22-23 0.2306917135520905 1105 24-25 0.11682078218986192 1105 26-27 0.1265849423749259 1105 28-29 0.4166910328295188 1105 30-31 0.5896963047335575 1105 32-33 1.0326221721973283 1105 34-35 1.112501555868981 1105 36-37 0.4314918853737524 1105 38-39 0.8379901924925051 1105 40-41 0.6163036270273352 1105 42-43 1.0761068711507207 1105 44-45 0.5576096573569131 1105 46-47 0.860918308723214 1105 48-49 0.8414264566486409 1105 50-51 1.5124906079070328 1105 52-53 1.4520868097281294 1105 54-55 1.1484699267668361 1105 56-57 1.1763364876772968 1105 58-59 1.257124915096199 1105 60-61 1.7673495674503812 1105 62-63 1.6647642515964094 1105 64-65 1.7903361000302098 1105 66-67 0.9349404610831016 1105 68-69 1.1284934747776418 1105 70-71 1.647545895502482 1105 72-73 2.1390600353514415 1105 74-75 1.7937835416218633 1105 76-77 1.1087624056432972 1105 78-79 1.3210180742302597 1105 80-81 1.8636529933899268 1105 82-83 1.5695160349865063 1105 84-85 1.5510366145635643 1105 86-87 1.5510869822951747 1105 88-89 1.5143475984374994 1105 90-91 1.3828113106862503 1105 92-93 1.725123619135104 1105 94-95 1.534260844429724 1105 96-97 2.0127570992690487 1105 98-99 1.9943714091327216 1105 100-101 1.7152142500796224 1106 1 0.20428414200781475 1106 2 0.26308279418558556 1106 3 0.12330697685963798 1106 4 0.12449816602288877 1106 5 0.31921602600407084 1106 6 0.4217482968087296 1106 7 -0.30839099409821813 1106 8 0.22692844210409646 1106 9 0.18727987610549235 1106 10-11 -0.018736706580241957 1106 12-13 -0.26468547048941815 1106 14-15 0.11782193968531374 1106 16-17 0.06144939735364119 1106 18-19 0.2727567522630636 1106 20-21 -0.15786396475968445 1106 22-23 -0.32937944134666 1106 24-25 -0.526173197011012 1106 26-27 -0.3156701205697843 1106 28-29 -0.2265766190588252 1106 30-31 -0.4907634653813844 1106 32-33 0.3723868136861057 1106 34-35 0.11469093736871372 1106 36-37 -0.5638556789393263 1106 38-39 -0.904894317633385 1106 40-41 -0.8500346324143777 1106 42-43 -0.5715395437370674 1106 44-45 -0.6974532873611992 1106 46-47 -0.8084850848181091 1106 48-49 -0.6551526347580392 1106 50-51 -0.7090474325965275 1106 52-53 -0.178318225849317 1106 54-55 -0.5701945505183375 1106 56-57 -0.6842272780588772 1106 58-59 -0.4003737165403649 1106 60-61 -1.766175336764178 1106 62-63 -1.2569653597883672 1106 64-65 -0.8462265710152153 1106 66-67 -1.3785516024089617 1106 68-69 -1.1997769138375745 1106 70-71 -2.238058921136819 1106 72-73 -1.9680828217914126 1106 74-75 -2.147924175947921 1106 76-77 -2.364007161953861 1106 78-79 -2.072523250345551 1106 80-81 -1.7463634269713282 1106 82-83 -1.5819617975258105 1106 84-85 -1.6917110592185942 1106 86-87 -1.587665070249983 1106 88-89 -2.1304252532319055 1106 90-91 -1.4575006761774638 1106 92-93 -0.5549803764861352 1106 94-95 -1.2212126093195934 1106 96-97 -2.025420240632428 1106 98-99 -2.3174786182345493 1106 100-101 -2.4250430022465963 1107 1 0.45502818962686575 1107 2 0.21323160370939576 1107 3 0.16745380225646045 1107 4 0.31943864221336327 1107 5 0.2004164228294698 1107 6 0.2702105983960337 1107 7 0.25361297415575024 1107 8 0.12375383892949543 1107 9 0.4152064634070811 1107 10-11 0.3379101188165805 1107 12-13 0.10337008506613898 1107 14-15 0.3303715428599219 1107 16-17 0.18086904021077999 1107 18-19 0.05289068083449422 1107 20-21 0.18414992412920128 1107 22-23 0.14879516182794816 1107 24-25 0.3697841442588299 1107 26-27 0.001046149271481056 1107 28-29 0.3318311190364156 1107 30-31 0.050903201285287025 1107 32-33 0.9636566549559475 1107 34-35 0.4947752627655362 1107 36-37 0.44792507502892676 1107 38-39 0.9338953649062987 1107 40-41 0.6295040580618121 1107 42-43 0.9532620435645143 1107 44-45 0.4797540539086427 1107 46-47 -0.032939449897476436 1107 48-49 0.5954674049244986 1107 50-51 1.4820487975622072 1107 52-53 1.2371083614522647 1107 54-55 1.0792350129737258 1107 56-57 1.0238580394014392 1107 58-59 1.2851421564755086 1107 60-61 1.6418107743469363 1107 62-63 1.415033648148139 1107 64-65 0.974333944857797 1107 66-67 1.4505654610831016 1107 68-69 1.3704115782259194 1107 70-71 1.7483002058473076 1107 72-73 1.3109350353514415 1107 74-75 1.8204538002425537 1107 76-77 1.6674908539191549 1107 78-79 1.7186473845750854 1107 80-81 1.7383835968381973 1107 82-83 1.396832845331332 1107 84-85 2.08147842490839 1107 86-87 1.6028111202262068 1107 88-89 1.5323971674030155 1107 90-91 0.6667552762034887 1107 92-93 1.1192507743075168 1107 94-95 1.1996703271883433 1107 96-97 1.7614101165104294 1107 98-99 1.2869360643051344 1107 100-101 1.4714103707692772 1108 1 0.6306910684147411 1108 2 0.4665459976487867 1108 3 0.44870380225646045 1108 4 0.35826439978912106 1108 5 0.4736171804052276 1108 6 0.32939620445664275 1108 7 0.3719841862769613 1108 8 0.35102656620222206 1108 9 0.3806420694676902 1108 10-11 0.3824176945741584 1108 12-13 0.23783978203584155 1108 14-15 0.38766320952658617 1108 16-17 0.31036714627138906 1108 18-19 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-0.016226050400256042 2316 86-87 0.1336753177570742 2316 88-89 0.2716911488844964 2316 90-91 0.3282136095368209 2316 92-93 0.5896404348013426 2316 94-95 0.20912248768216912 2316 96-97 0.44283372762154016 2316 98-99 0.9020981013421725 2316 100-101 0.9479728707692772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 2.0 10 1.0 11 1.0 12 1.0 13 5.0 14 5.0 15 16.0 16 45.0 17 99.0 18 159.0 19 231.0 20 324.0 21 416.0 22 544.0 23 575.0 24 554.0 25 645.0 26 944.0 27 1057.0 28 1337.0 29 1408.0 30 937.0 31 788.0 32 922.0 33 1261.0 34 1867.0 35 2722.0 36 4966.0 37 12793.0 38 24437.0 39 7713.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.73253774306232 20.456964663198452 14.09873914933809 25.711758444401134 2 13.436947863324649 20.439912556523822 43.39681969274998 22.72631988740155 3 15.974904920192856 27.71838409247447 33.487557272482256 22.81915371485042 4 11.190968166981104 20.490821429640942 38.461653639984426 29.856556763393527 5 11.70155421794987 37.629143832539754 37.74593477674962 12.923367172760758 6 26.117749228880307 40.658221783008415 19.343874464708172 13.880154523403107 7 23.328242446021623 33.64178121163118 22.70236277064055 20.327613571706646 8 22.62899410056 37.07513550744168 22.86407330877729 17.431797083221035 9 24.388344862695774 15.545174138292456 19.840984637498877 40.22549636151289 10-11 22.043542059713115 26.67400353367472 31.08959961668613 20.19285478992603 12-13 24.14577905549067 23.82834725840745 29.58179858054083 22.444075105561044 14-15 21.23124606953553 25.24705776659779 26.391758751834217 27.12993741203246 16-17 21.28215494265265 29.458269697241935 26.841703351001705 22.417872009103704 18-19 21.505255592489444 27.217530620189862 28.84586590003893 22.431347887281767 20-21 21.73659150121283 26.18288264007427 28.60404875273261 23.476477105980294 22-23 20.817237145509537 28.258167879495705 28.75752403198275 22.167070943012007 24-25 21.942472973377654 28.651962986254603 28.597310813643578 20.808253226724162 26-27 22.230707034408407 28.520947503967896 28.61153535172042 20.636810109903273 28-29 20.835953642979067 28.085976102776034 29.23142574791124 21.84664450633366 30-31 23.512412781121792 27.529721797981615 28.546401940526454 20.411463480370138 32-33 20.01991435330758 29.22169316922708 29.102656245320873 21.655736232144463 34-35 21.461084658461356 28.637738448177764 29.661156529811638 20.240020363549245 36-37 20.156170454885753 29.229928428113677 29.197736052466084 21.416165064534486 38-39 20.490072769742163 26.484592579283085 30.749708022639478 22.275626628335278 40-41 20.35755996765789 26.52951217320995 31.052166621747073 22.060761237385083 42-43 21.69915850627377 25.89090527954961 31.045428682658045 21.36450753151858 44-45 20.17937891174797 27.217530620189862 30.963824753690893 21.639265714371277 46-47 21.58001916626737 25.678306181121226 29.589422616195492 23.152252036415906 48-49 21.273648825700576 25.676616580194505 29.722465542154243 23.327269051950676 50-51 20.131464678225974 26.656784356002756 30.452490042823342 22.759260922947924 52-53 19.910610008085527 27.015392447518945 31.70649537328182 21.367502171113706 54-55 19.933818464947745 27.00715718863235 30.12083370766328 22.938190638756627 56-57 21.089749348665887 28.21774024496152 27.989398975833257 22.703111430539337 58-59 19.50034438355344 29.13559728086725 30.26981702752074 21.094241308058574 60-61 20.663761866259396 30.96831671308358 27.201060102416676 21.16686131824035 62-63 19.28023837331177 32.23130596232743 27.055820082053124 21.432635582307668 64-65 19.544515317581528 31.92435540382715 26.90009882310664 21.63103045548468 66-67 19.611146048573055 31.79259126164166 26.665019614889346 21.93124307489594 68-69 19.69664073788472 31.352613965606306 26.774524410238747 22.176220886270222 70-71 19.86119845476597 30.84703380948103 26.850687269787084 22.44108046596592 72-73 20.05959332794298 30.045219057886385 26.948013056628632 22.947174557542 74-75 20.075315185817384 29.761476956248316 27.15539184859102 23.007816009343276 76-77 20.087293744197886 29.84682418470937 26.99368131045429 23.072200760638456 78-79 20.686970323121614 29.57356332165424 26.658281675800318 23.08118467942383 80-81 20.365046566645702 29.632707453657954 26.639565178330788 23.362680801365556 82-83 20.50205132812266 29.412601443416285 26.548228670679485 23.53711855778157 84-85 20.690713622615515 29.033030874734227 26.714431168208908 23.56182433444135 86-87 20.38076842452011 28.976881382325637 26.96148893480669 23.68086125834756 88-89 20.832959003383944 28.61153535172042 26.79378911747971 23.761716527415924 90-91 20.949906791144784 28.37442259173024 26.823187667981824 23.852482949143152 92-93 20.966969125265773 28.132393016500462 26.712933848411343 24.187704009822415 94-95 20.99466954152068 28.04030784895038 26.859671188572452 24.105351420956488 96-97 21.014134698888988 27.841912975773365 27.102236995777556 24.041715329560088 98-99 21.04514486785955 27.858051957774947 26.866062738638917 24.230740435726585 100-101 20.95814211168592 28.036025784039946 26.714631169939583 24.291200934334547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.0 2 4.5 3 6.5 4 6.0 5 4.5 6 8.5 7 20.0 8 41.5 9 51.5 10 35.5 11 31.0 12 40.5 13 53.0 14 52.0 15 51.5 16 61.0 17 71.0 18 82.5 19 77.0 20 88.5 21 111.5 22 133.5 23 164.5 24 189.0 25 236.0 26 311.5 27 409.5 28 503.5 29 610.0 30 761.5 31 923.0 32 1090.0 33 1247.5 34 1421.5 35 1640.0 36 1915.0 37 2165.5 38 2308.0 39 2424.0 40 2603.5 41 2749.5 42 2781.0 43 2831.0 44 2991.5 45 3127.5 46 3150.5 47 3089.5 48 3030.5 49 3014.5 50 2849.0 51 2563.5 52 2309.0 53 2122.0 54 1848.5 55 1500.5 56 1212.5 57 926.0 58 739.5 59 607.0 60 431.0 61 300.0 62 198.0 63 124.5 64 98.0 65 66.0 66 40.5 67 34.0 68 25.0 69 16.5 70 15.0 71 11.5 72 8.5 73 6.5 74 4.0 75 2.0 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1272721827928009 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0029946395951247267 48-49 0.0022459796963435453 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.486598987811817E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.486598987811817E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014973197975623634 100-101 7.486598987811817E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 66786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.61695564938759 #Duplication Level Percentage of deduplicated Percentage of total 1 94.92808740146592 82.22381936333963 2 3.927534227631033 6.803821160123379 3 0.6240492324713042 1.6215973407600397 4 0.20571151984511132 0.712724223639685 5 0.11409210344350712 0.4941155331955799 6 0.053588715253768494 0.2785014823465996 7 0.020744018807910387 0.12577486299523855 8 0.02420135527589545 0.1676998173269847 9 0.012100677637947725 0.0943311472464289 >10 0.06914672935970129 1.030156020722906 >50 0.005186004701977597 0.3054532387027221 >100 0.010372009403955194 1.7159284880064685 >500 0.0034573364679850644 1.763842721528464 >1k 0.0017286682339925322 2.662234600065882 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1778 2.662234600065882 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 676 1.0121881831521577 RNA PCR Primer, Index 17 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 502 0.7516545383763065 RNA PCR Primer, Index 17 (95% over 23bp) TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 280 0.4192495433174618 RNA PCR Primer, Index 17 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.41026562453208754 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 204 0.3054532387027221 RNA PCR Primer, Index 17 (96% over 26bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 158 0.23657652801485343 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 116 0.17368909651723413 TruSeq Adapter, Index 12 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.17069445692210944 No Hit ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC 74 0.1108016650196149 TruSeq Adapter, Index 25 (95% over 24bp) CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA 72 0.10780702542449017 RNA PCR Primer, Index 17 (96% over 28bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0014973197975623634 0.0 24-25 0.0 0.0 0.0 0.0029946395951247267 0.0 26-27 0.0 0.0 0.0 0.00898391878537418 0.0 28-29 0.0 0.0 0.0 0.03443835534393436 0.0 30-31 0.0 0.0 0.0 0.11753960410864553 0.0 32-33 0.0 0.0 0.0 0.23058724882460396 0.0 34-35 0.0 0.0 0.0 0.41550624382355583 0.0 36-37 0.0 0.0 0.0 0.6887671068786871 0.0 38-39 0.0 0.0 0.0 1.0863055131314947 0.0 40-41 0.0 0.0 0.0 1.536998772197766 0.0 42-43 0.0 0.0 0.0 1.989938010960381 0.0 44-45 0.0 0.0 0.0 2.45186116850837 0.0 46-47 0.0 0.0 0.0 2.9444793819063877 0.0 48-49 0.0 0.0 0.0 3.461054712065403 0.0 50-51 0.0 0.0 0.0 3.930464468601204 0.0 52-53 0.0 0.0 0.0 4.430569280987033 0.0 54-55 0.0 0.0 0.0 4.911208936004552 0.0 56-57 0.0 0.0 0.0 5.406073129098913 0.0 58-59 0.0 0.0 0.0 5.898691342496931 0.0 60-61 0.0 0.0 0.0 6.3598658401461385 0.0 62-63 0.0 0.0 0.0 6.763393525589195 0.0 64-65 0.0 0.0 0.0 7.256760398885994 0.0 66-67 0.0 0.0 0.0 7.839966460036535 0.0 68-69 0.0 0.0 0.0 8.44563231815051 0.0 70-71 0.0 0.0 0.0 9.026592399604707 0.0 72-73 0.0 0.0 0.0 9.563381547030815 0.0 74-75 0.0 0.0 0.0 10.076213577695924 0.0 76-77 0.0 0.0 0.0 10.610008085526907 0.0 78-79 0.0 0.0 0.0 11.164016410624981 0.0 80-81 0.0 0.0 0.0 11.756206390560894 0.0 82-83 0.0 0.0 0.0 12.352139669990716 0.0 84-85 0.0 0.0 0.0 12.933099751444914 0.0 86-87 0.0 0.0 0.0 13.50732189381008 0.0 88-89 0.0 0.0 0.0 14.10924445243015 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 105 0.0 58.853146 1 GTATCAA 210 0.0 47.535233 1 AACGCAG 240 0.0 41.562172 6 ATCAACG 245 0.0 40.713963 3 TATCAAC 250 0.0 39.899685 2 GTATAAA 60 8.1712205E-5 39.612694 1 CAACGCA 255 0.0 39.11734 5 TCAACGC 255 0.0 39.11734 4 GACAGCC 50 0.0016049952 37.999702 7 CGCAGAG 275 0.0 36.272438 8 ACGCAGA 280 0.0 35.62472 7 GCAGAGT 285 0.0 34.999725 9 CAGTACT 55 0.0025624272 34.545185 4 CAGAGTA 260 0.0 28.317083 10-11 GAGTACT 175 0.0 27.142645 12-13 GTACTTT 200 0.0 23.749813 14-15 AGAGTAC 265 0.0 23.3017 10-11 ACTTTTT 225 0.0 21.110945 16-17 GTACATG 140 1.3202325E-5 20.372242 1 ACATGGG 165 6.2097286E-5 20.151356 3 >>END_MODULE