FastQCFastQC Report
Fri 10 Feb 2017
SRR2031494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609812
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36570.22716938375412782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25780.1601429235215044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.051.1695251
GTATCAA42750.044.5555571
TCAACGC51100.036.438364
CAACGCA51750.036.0724645
ATCAACG52100.035.7389643
AACGCAG52750.035.2985766
TATCAAC55750.033.739912
ACGCAGA59250.031.3459917
CGCAGAG60000.030.9541668
GTACATG51250.027.8975621
TACATGG51750.026.9855062
GCAGAGT70250.025.9644139
ACATGGG53000.025.5424543
ACCGTAC1201.7112304E-423.758
AGAGTAC60400.022.41307810-11
GAGTACT38750.022.06451612-13
CAGAGTA65800.021.65653410-11
CATGGGG33850.021.3293954
CATGGGA21100.020.7109014
AGTACTT44750.018.68156612-13
Produced by FastQC (version 0.11.5)