##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031494_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1609812 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19716588023943 34.0 31.0 34.0 30.0 34.0 2 32.35862510653418 34.0 31.0 34.0 30.0 34.0 3 32.46201357674064 34.0 31.0 34.0 30.0 34.0 4 35.695584329101784 37.0 35.0 37.0 35.0 37.0 5 35.67770522272166 37.0 35.0 37.0 35.0 37.0 6 35.80083761333621 37.0 37.0 37.0 35.0 37.0 7 35.828082409622986 37.0 37.0 37.0 35.0 37.0 8 35.87515125989867 37.0 37.0 37.0 35.0 37.0 9 37.70183909673925 39.0 39.0 39.0 35.0 39.0 10-11 37.69849926575277 39.0 39.0 39.0 35.0 39.0 12-13 37.67262233105481 39.0 39.0 39.0 35.0 39.0 14-15 39.18894846106254 41.0 39.0 41.0 36.0 41.0 16-17 39.200037644147265 41.0 39.0 41.0 36.0 41.0 18-19 39.152571542515524 41.0 39.5 41.0 36.0 41.0 20-21 39.110909534777974 41.0 39.5 41.0 36.0 41.0 22-23 39.037707198107604 41.0 39.0 41.0 36.0 41.0 24-25 38.98505881432118 41.0 39.0 41.0 35.5 41.0 26-27 38.87602123726249 41.0 39.0 41.0 35.0 41.0 28-29 38.77831448641208 41.0 39.0 41.0 35.0 41.0 30-31 38.607701706783146 40.0 38.5 41.0 35.0 41.0 32-33 38.490247618976625 40.0 38.0 41.0 34.0 41.0 34-35 38.35650001366619 40.0 38.0 41.0 34.0 41.0 36-37 38.20757423848251 40.0 38.0 41.0 34.0 41.0 38-39 38.059142309785244 40.0 38.0 41.0 33.0 41.0 40-41 37.91933374828862 40.0 38.0 41.0 33.0 41.0 42-43 37.761992083547646 40.0 38.0 41.0 33.0 41.0 44-45 37.919130929574386 40.0 38.0 41.0 33.0 41.0 46-47 37.78059239215511 40.0 38.0 41.0 33.0 41.0 48-49 37.641090077599124 40.0 38.0 41.0 32.0 41.0 50-51 36.57236869895367 39.0 36.5 40.0 30.5 40.5 52-53 36.63234619943198 39.0 36.5 40.0 30.5 40.5 54-55 37.389161280944606 40.0 37.0 41.0 32.0 41.0 56-57 37.40999073183701 40.0 37.0 41.0 32.0 41.0 58-59 37.20513016426763 40.0 36.5 41.0 31.5 41.0 60-61 37.021809378983384 40.0 36.0 41.0 31.0 41.0 62-63 36.75166789662396 39.0 35.0 41.0 31.0 41.0 64-65 36.46677624467951 39.0 35.0 41.0 31.0 41.0 66-67 36.16049793392023 38.5 35.0 41.0 30.0 41.0 68-69 35.84738279997913 37.0 35.0 40.5 30.0 41.0 70-71 35.47928360578751 37.0 35.0 40.0 29.5 41.0 72-73 35.119709320094515 36.5 35.0 39.0 29.0 41.0 74-75 34.74581379689057 36.0 35.0 39.0 29.0 41.0 76-77 34.39098478580107 35.5 34.5 38.0 29.0 40.5 78-79 34.01377241566096 35.0 34.0 37.0 28.5 39.0 80-81 33.691225435019746 35.0 34.0 37.0 28.0 39.0 82-83 33.4049680956534 35.0 34.0 36.0 27.0 39.0 84-85 33.12138156505232 35.0 34.0 36.0 27.0 37.0 86-87 32.86782276439733 35.0 34.0 36.0 26.0 37.0 88-89 32.658312585569 35.0 34.0 35.0 26.0 36.5 90-91 32.48336203233669 35.0 34.0 35.0 25.5 36.0 92-93 32.33147504180612 35.0 34.0 35.0 25.0 36.0 94-95 32.20271870255657 35.0 33.0 35.0 25.0 36.0 96-97 32.07842468561546 35.0 33.0 35.0 25.0 35.5 98-99 31.943049871662033 35.0 33.0 35.0 24.0 35.0 100-101 30.35624594673167 33.5 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07239885428634096 1101 2 0.0771012649539955 1101 3 0.23160176702680957 1101 4 -0.05313209092331306 1101 5 -0.069476555671379 1101 6 -0.013529651854248925 1101 7 -0.05395668360900885 1101 8 0.14509161605388243 1101 9 0.3687703645355569 1101 10-11 0.5201328172430806 1101 12-13 0.578717142526969 1101 14-15 0.642638586523347 1101 16-17 0.49560875312604225 1101 18-19 0.626774574250625 1101 20-21 0.60160721391793 1101 22-23 0.5545775869826883 1101 24-25 0.575553829869051 1101 26-27 0.5337226962671906 1101 28-29 0.6827335768958065 1101 30-31 0.7453273909443183 1101 32-33 0.7106254242220231 1101 34-35 0.7264019493485705 1101 36-37 0.8078168563278396 1101 38-39 0.7012450933421235 1101 40-41 0.604305314224483 1101 42-43 0.641572822250545 1101 44-45 0.8299549673285114 1101 46-47 0.8757431480293292 1101 48-49 0.7515390723323705 1101 50-51 0.7714588930391102 1101 52-53 0.7312087882725038 1101 54-55 0.7334867423282887 1101 56-57 0.7837406672196892 1101 58-59 0.6606992914671466 1101 60-61 0.7006421954156181 1101 62-63 0.6417550243414922 1101 64-65 0.7772616318573284 1101 66-67 0.899248377899454 1101 68-69 0.7823152135987499 1101 70-71 0.7813099480269798 1101 72-73 0.731534827634313 1101 74-75 0.802293455093384 1101 76-77 0.7928641604381923 1101 78-79 0.8835419058759086 1101 80-81 0.783246992519544 1101 82-83 0.8019281284572912 1101 84-85 0.6516827349830194 1101 86-87 0.6252605630777524 1101 88-89 0.6419910775975168 1101 90-91 0.5795376679805955 1101 92-93 0.607000437918984 1101 94-95 0.6762942301885886 1101 96-97 0.7308134235446673 1101 98-99 0.7294253989743105 1101 100-101 0.37922531393023107 1102 1 0.09168570657372044 1102 2 0.0752493569045285 1102 3 0.23730694872774905 1102 4 -0.019381996089840925 1102 5 0.05090505422165137 1102 6 0.0254431119724714 1102 7 0.08750906254074664 1102 8 0.188557210470087 1102 9 0.2721661615160613 1102 10-11 0.3283366712754088 1102 12-13 0.35282950122509504 1102 14-15 0.38535272050408054 1102 16-17 0.37729489226571644 1102 18-19 0.43407333762214506 1102 20-21 0.5352552679350779 1102 22-23 0.47287399848408995 1102 24-25 0.41869205913845775 1102 26-27 0.47477800314830887 1102 28-29 0.5461619965908184 1102 30-31 0.6069046612576514 1102 32-33 0.6426222378173421 1102 34-35 0.5618036639377522 1102 36-37 0.6049525061267857 1102 38-39 0.5914074482469047 1102 40-41 0.5743011415516932 1102 42-43 0.5182327873518204 1102 44-45 0.6753889252983143 1102 46-47 0.647024537908699 1102 48-49 0.5499697680307278 1102 50-51 0.5090861595591036 1102 52-53 0.4604027037569125 1102 54-55 0.507500094881209 1102 56-57 0.45261025222841766 1102 58-59 0.6979999515081161 1102 60-61 0.5888521187901787 1102 62-63 0.5056739227558751 1102 64-65 0.6501495007595395 1102 66-67 0.6603082367872446 1102 68-69 0.5748279212840472 1102 70-71 0.4530799199562736 1102 72-73 0.39883283233805145 1102 74-75 0.4943111320526441 1102 76-77 0.510330589388964 1102 78-79 0.5517184478681756 1102 80-81 0.352414733965567 1102 82-83 0.4113504029432917 1102 84-85 0.4990884705114311 1102 86-87 0.5033764116476576 1102 88-89 0.4238141562713622 1102 90-91 0.3480074350695901 1102 92-93 0.3795598036727199 1102 94-95 0.30455915697714886 1102 96-97 0.3398138787453391 1102 98-99 0.4336363844837585 1102 100-101 0.34358651565998954 1103 1 0.3383881770355899 1103 2 0.345990495465216 1103 3 0.3202285384162522 1103 4 0.2846351897318513 1103 5 0.2086923796781477 1103 6 0.1920381710487291 1103 7 0.22675718284150292 1103 8 0.24943798383205973 1103 9 0.32451847086085195 1103 10-11 0.3967469827684198 1103 12-13 0.4718950221703153 1103 14-15 0.4514214637908651 1103 16-17 0.5115806065514263 1103 18-19 0.6069734450335389 1103 20-21 0.5914421637460379 1103 22-23 0.5119824839835516 1103 24-25 0.6036759474306095 1103 26-27 0.6456158119560413 1103 28-29 0.5623811158174519 1103 30-31 0.5854760898290792 1103 32-33 0.5441259972158363 1103 34-35 0.48379077457147446 1103 36-37 0.4692704438281865 1103 38-39 0.624619048676557 1103 40-41 0.695364514269194 1103 42-43 0.6762564178566564 1103 44-45 0.5423169596699537 1103 46-47 0.5635551501535971 1103 48-49 0.5964359334442619 1103 50-51 0.8136081788931548 1103 52-53 0.6862029185796885 1103 54-55 0.662097302185181 1103 56-57 0.7838454831628923 1103 58-59 0.8043264821203593 1103 60-61 0.8590239770071477 1103 62-63 0.815244277213445 1103 64-65 0.8091613160119593 1103 66-67 0.7435198372168941 1103 68-69 0.7968902198877075 1103 70-71 0.7292345923515384 1103 72-73 0.7182420697172134 1103 74-75 0.7083175767357801 1103 76-77 0.6971189889593106 1103 78-79 0.8083672126372363 1103 80-81 0.8003739176390425 1103 82-83 0.7708348282923794 1103 84-85 0.6758553878046669 1103 86-87 0.6880488069215573 1103 88-89 0.6801943925764107 1103 90-91 0.5997797229321051 1103 92-93 0.7464985791829193 1103 94-95 0.6827439045603896 1103 96-97 0.8356677992609107 1103 98-99 0.7520789193924564 1103 100-101 0.7970559034150959 1104 1 0.092976776193062 1104 2 0.16573133693058395 1104 3 0.1932195265248211 1104 4 0.07405554914101486 1104 5 0.035360112840841396 1104 6 0.045981540109686136 1104 7 0.08728616487104546 1104 8 0.052621474473561136 1104 9 0.3413744405723662 1104 10-11 0.28215198463479396 1104 12-13 0.35790259429210636 1104 14-15 0.45591141206580943 1104 16-17 0.3817427139475811 1104 18-19 0.5393895917833902 1104 20-21 0.49492960575531697 1104 22-23 0.4506696273739479 1104 24-25 0.45670479614815207 1104 26-27 0.39586643852246084 1104 28-29 0.4468929272609543 1104 30-31 0.5181002033042574 1104 32-33 0.47818454784773934 1104 34-35 0.5045927795840086 1104 36-37 0.5073363532826747 1104 38-39 0.6425841742564629 1104 40-41 0.537659080471947 1104 42-43 0.48987557212530675 1104 44-45 0.5666597314931323 1104 46-47 0.5748520093227967 1104 48-49 0.5797266069365037 1104 50-51 0.5017754054702337 1104 52-53 0.6442469648656157 1104 54-55 0.6002298676413815 1104 56-57 0.604588830893924 1104 58-59 0.7005777485843865 1104 60-61 0.5668547240876123 1104 62-63 0.5212753122478446 1104 64-65 0.5372981474522618 1104 66-67 0.5277463609730972 1104 68-69 0.4885795495949523 1104 70-71 0.43518297394381733 1104 72-73 0.46388276720561805 1104 74-75 0.4201050240775359 1104 76-77 0.40372326560838445 1104 78-79 0.4708398836765397 1104 80-81 0.47990785887510157 1104 82-83 0.32110145229921017 1104 84-85 0.37628198621558084 1104 86-87 0.5014759919052949 1104 88-89 0.6301623976955923 1104 90-91 0.6060187246918218 1104 92-93 0.5099737564878879 1104 94-95 0.5207351592929683 1104 96-97 0.404824734151326 1104 98-99 0.47311821977106305 1104 100-101 0.39806907464103247 1105 1 0.2716814519184183 1105 2 0.2367129583284182 1105 3 0.3037760102926086 1105 4 0.32249147046147186 1105 5 0.2904988764621521 1105 6 0.28243563796132065 1105 7 0.2533702756847944 1105 8 0.17018390701388597 1105 9 0.3292925673250835 1105 10-11 0.3607662212746945 1105 12-13 0.39133129527140653 1105 14-15 0.458475495673909 1105 16-17 0.4455426833905065 1105 18-19 0.5093625832717663 1105 20-21 0.43631694625477735 1105 22-23 0.5029382437791483 1105 24-25 0.383049677098775 1105 26-27 0.3307749397964912 1105 28-29 0.3443835506745714 1105 30-31 0.4258166040750879 1105 32-33 0.4336278965089022 1105 34-35 0.5073145771285965 1105 36-37 0.5164199367404549 1105 38-39 0.4958495920258912 1105 40-41 0.4657671539469206 1105 42-43 0.5325031848784505 1105 44-45 0.5244941712883033 1105 46-47 0.5973434952345045 1105 48-49 0.6382830808471667 1105 50-51 0.62400099554214 1105 52-53 0.6111837166485188 1105 54-55 0.7162459643200165 1105 56-57 0.7436964657270195 1105 58-59 0.7118565128957002 1105 60-61 0.7670765093108045 1105 62-63 0.6958550292498984 1105 64-65 0.6805637623641161 1105 66-67 0.6278993927770955 1105 68-69 0.5979780680696578 1105 70-71 0.7655332960252466 1105 72-73 0.6357153045764292 1105 74-75 0.6921006968014112 1105 76-77 0.6774917415496162 1105 78-79 0.6630746901566127 1105 80-81 0.7639647048920892 1105 82-83 0.7437237914925987 1105 84-85 0.6033533369861672 1105 86-87 0.7376459582432275 1105 88-89 0.7483413931563874 1105 90-91 0.7905100233182267 1105 92-93 0.7802720329702808 1105 94-95 0.804446266789796 1105 96-97 0.8659990129797137 1105 98-99 0.8880764009350948 1105 100-101 0.8862646793507629 1106 1 0.09368025338029895 1106 2 0.12904428378216437 1106 3 0.0991373489040086 1106 4 0.1281285384883617 1106 5 0.17383600797966636 1106 6 0.11046886316363214 1106 7 0.14730856128761616 1106 8 -0.029760011155410382 1106 9 0.061399134057303684 1106 10-11 0.05329219194521073 1106 12-13 0.1359397493729304 1106 14-15 0.15231793573997265 1106 16-17 0.14512600851788449 1106 18-19 0.10997132434720669 1106 20-21 0.18924436154823354 1106 22-23 0.07199790715687726 1106 24-25 0.158139028209483 1106 26-27 0.3064602306952011 1106 28-29 0.19764234581553097 1106 30-31 0.22683911277105295 1106 32-33 0.13538298584125386 1106 34-35 0.10681950023178644 1106 36-37 0.16510618703235735 1106 38-39 0.2502118769009556 1106 40-41 0.10741386842381218 1106 42-43 0.05971506447319541 1106 44-45 0.07431040482129703 1106 46-47 0.20027194213354704 1106 48-49 0.25621036953448595 1106 50-51 -0.06398966186257837 1106 52-53 -0.010625691406822568 1106 54-55 0.03318849169742322 1106 56-57 0.1145279585822081 1106 58-59 0.17514776609953486 1106 60-61 0.126654592006183 1106 62-63 -0.037281267417355934 1106 64-65 0.046022700241763914 1106 66-67 0.19267156268441 1106 68-69 0.10092916340033042 1106 70-71 0.219373400911401 1106 72-73 0.2170756922350492 1106 74-75 0.14909259119500717 1106 76-77 0.19458380484518045 1106 78-79 0.13098144051462413 1106 80-81 0.09765558334175495 1106 82-83 0.16794298329526924 1106 84-85 0.2783809324354749 1106 86-87 0.11421036466206402 1106 88-89 0.14504883637822985 1106 90-91 0.2772522504046293 1106 92-93 0.13435283019506983 1106 94-95 0.14963227934350698 1106 96-97 0.1511680884454094 1106 98-99 0.07233147385553806 1106 100-101 -0.011234052243281667 1107 1 0.22494917412290505 1107 2 0.15431536168481585 1107 3 0.11930731644240211 1107 4 0.10447533062462355 1107 5 0.17335403381348158 1107 6 0.145814503772435 1107 7 0.13420146923315457 1107 8 0.158040571382017 1107 9 0.20118987911120456 1107 10-11 0.16481580346882652 1107 12-13 0.16298761852606702 1107 14-15 0.25813080471073135 1107 16-17 0.10707610204692486 1107 18-19 0.18913492982660074 1107 20-21 0.14483239608363618 1107 22-23 0.20911043900624549 1107 24-25 0.2736617226795417 1107 26-27 0.2559050485610683 1107 28-29 0.11756298942037802 1107 30-31 0.13116463551762791 1107 32-33 0.20787320306830992 1107 34-35 0.3157253407165683 1107 36-37 0.3333529473843768 1107 38-39 0.3263090845771188 1107 40-41 0.3537808253170951 1107 42-43 0.31967239021999916 1107 44-45 0.2572495614446666 1107 46-47 0.42121280781125137 1107 48-49 0.3664691245300844 1107 50-51 0.21385541861407376 1107 52-53 0.1162273038672268 1107 54-55 0.07725004891687348 1107 56-57 0.3056567681320743 1107 58-59 0.31681039460426774 1107 60-61 0.2867690152785016 1107 62-63 0.2863578720112585 1107 64-65 0.33643558275990415 1107 66-67 0.360010387917967 1107 68-69 0.23986928918256467 1107 70-71 0.3005886531792825 1107 72-73 0.2009894787804214 1107 74-75 0.24810962442256113 1107 76-77 0.30759111522090876 1107 78-79 0.12988722889409843 1107 80-81 0.08576645303157449 1107 82-83 0.16121618709479435 1107 84-85 0.22397907540203477 1107 86-87 0.3123799049368685 1107 88-89 0.3561105342820312 1107 90-91 0.2418206360256434 1107 92-93 0.262650445334792 1107 94-95 0.30937805751032954 1107 96-97 0.2746199092656596 1107 98-99 0.2028917623105677 1107 100-101 0.406000562359754 1108 1 0.19804358514834774 1108 2 0.13741812409792686 1108 3 0.1643749546134856 1108 4 0.03986181428697222 1108 5 0.17201054971138063 1108 6 0.2636364457323097 1108 7 0.18680149424697134 1108 8 0.23097804087121432 1108 9 0.21709866743378825 1108 10-11 0.193075971384161 1108 12-13 0.2527079479015626 1108 14-15 0.2536759243937823 1108 16-17 0.21619323530199352 1108 18-19 0.21463248291035342 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-0.5579824071038004 2316 84-85 -0.41456537564241813 2316 86-87 -0.47653332147636007 2316 88-89 -0.4771918615203745 2316 90-91 -0.48160402489901344 2316 92-93 -0.31549775781341083 2316 94-95 -0.41274722711495215 2316 96-97 -0.3233716262258781 2316 98-99 -0.41659516967636634 2316 100-101 -0.39534336398417125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 24.0 7 584.0 8 1420.0 9 1098.0 10 1129.0 11 1519.0 12 1875.0 13 2060.0 14 2302.0 15 2501.0 16 3041.0 17 3691.0 18 4665.0 19 6122.0 20 7658.0 21 9688.0 22 10742.0 23 11158.0 24 10574.0 25 10002.0 26 10219.0 27 11291.0 28 12778.0 29 14817.0 30 17900.0 31 22157.0 32 28330.0 33 37990.0 34 55539.0 35 89856.0 36 171141.0 37 361516.0 38 504667.0 39 179606.0 40 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0855901185977 19.781440317254436 13.837764906709605 23.29520465743826 2 14.839124071630724 21.379266647285522 40.73127793804494 23.050331343038817 3 17.13361560231878 27.413635877978297 31.27265792527326 24.18009059442966 4 12.03121855222846 18.082359927743116 39.98162518356181 29.904796336466617 5 12.714217560808343 38.61519233301777 35.7025540870611 12.968036019112791 6 29.001150444896673 39.015176927492156 19.06992866247736 12.913743965133817 7 25.747230111342194 33.63448651146842 23.98292471419023 16.63535866299916 8 24.973972115998638 35.51420911261688 21.660790204073518 17.851028567310966 9 26.480856149662195 15.956024678658128 20.9977935311701 36.56532564050958 10-11 23.563931688917712 26.480234959113236 30.24399743572541 19.711835916243636 12-13 24.750157161208886 24.624490313154578 29.541772579655262 21.08357994598127 14-15 22.21327707831722 25.786178758761892 27.070645516370856 24.929898646550033 16-17 21.69613594630926 28.88706880058044 27.50762200803572 21.90917324507458 18-19 21.70200619699692 27.987429588051278 29.33997882982609 20.970585385125716 20-21 22.550397189237003 27.290174256372797 29.05727501099507 21.10215354339513 22-23 22.487191050880476 27.163637741549945 28.69704661165403 21.65212459591555 24-25 22.074254633460306 27.345957167669265 28.715216435211065 21.86457176365936 26-27 21.935014771911256 27.58561248145746 28.73450440175623 21.744868344875055 28-29 22.006420625514036 27.5648336575948 28.768887298641083 21.65985841825008 30-31 22.30521327956308 27.416120640174128 28.671236144344803 21.60742993591798 32-33 22.076490919436555 27.58443221941444 28.53218263996044 21.80689422118856 34-35 22.228061413382434 27.522375283573485 28.61396237573083 21.635600927313252 36-37 22.381743955194768 27.36207706241474 28.414125376130876 21.84205360625961 38-39 22.13525554536803 27.68745667195921 28.394091980926966 21.783195801745794 40-41 22.088635194668694 27.471313420449096 28.63284656841917 21.80720481646304 42-43 22.10410283933776 27.416213818756475 28.327251877859027 22.152431464046735 44-45 22.175601871522886 27.57036225348053 28.187328706706126 22.06670716829046 46-47 22.18712835276472 27.565901431194188 28.10301467347534 22.143955542565752 48-49 22.050017207042337 27.707003204112794 28.022600238786538 22.22037935005833 50-51 22.10158701761448 27.813371996233098 27.840704380387276 22.244336605765145 52-53 22.13749183134428 27.763179799877253 27.78895920765903 22.310369161119432 54-55 22.114973673944537 27.59368795859392 27.853345608058582 22.43799275940296 56-57 22.01176286423508 27.73519516564667 27.78299577838903 22.47004619172922 58-59 22.098574243452028 27.517250461544577 27.846232976273004 22.537942318730387 60-61 22.119011412512766 27.60545951949669 27.94916425023543 22.326364817755117 62-63 21.972876335870275 27.572443241819546 28.054083333954523 22.40059708835566 64-65 22.032168973768364 27.395497113948707 27.91794942515027 22.65438448713266 66-67 22.018409603108935 27.477742742630817 27.77249765811163 22.73134999614862 68-69 21.930324783266617 27.558590692577766 27.76485701435944 22.746227509796174 70-71 22.11609181693266 27.2892735300768 27.836356046544562 22.75827860644597 72-73 22.06698670403749 27.28445930332238 27.73215133195677 22.91640266068336 74-75 22.048195689931497 27.427208891473043 27.663851431098784 22.860743987496676 76-77 22.041020939091023 27.34949795379833 27.706030269373073 22.903450837737573 78-79 22.064253465622073 27.356206811727084 27.735536820448598 22.844002902202245 80-81 22.12056438888516 27.397174328430896 27.536880082891667 22.945381199792276 82-83 22.0863057301101 27.30623203206337 27.631518463025493 22.97594377480103 84-85 22.137150176542352 27.221284224493296 27.60086270943439 23.040702889529957 86-87 22.13478965245631 27.31045612779629 27.654160858535032 22.900593361212366 88-89 22.213463435481906 26.960570551095408 27.69646393491911 23.129502078503577 90-91 22.279992943275364 26.904973996963623 27.805203340514296 23.009829719246717 92-93 22.42889231786072 26.839966406015115 27.71696322303474 23.01417805308943 94-95 22.382706800545655 26.56136244480722 27.744109250024223 23.311821504622902 96-97 22.33903710495387 26.506635557443975 27.854277393882022 23.300049943720136 98-99 22.47141281093693 26.583010935438423 27.69407235130562 23.25150390231903 100-101 22.63897896151849 26.443864252471716 27.690562624704 23.22659416130579 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 90.0 1 63.5 2 68.5 3 99.0 4 85.0 5 108.5 6 163.0 7 307.0 8 581.0 9 763.5 10 710.0 11 658.0 12 753.5 13 850.5 14 952.5 15 1079.0 16 1206.5 17 1303.5 18 1425.0 19 1595.5 20 1797.0 21 2106.5 22 2535.5 23 3145.5 24 3990.5 25 5426.5 26 7696.0 27 10961.5 28 14787.0 29 19468.0 30 25015.0 31 29729.5 32 34617.0 33 39722.5 34 43286.5 35 46364.0 36 50496.5 37 51894.5 38 50291.0 39 49539.5 40 49371.5 41 49409.0 42 48823.0 43 49125.5 44 50627.0 45 52055.5 46 53325.0 47 55111.0 48 56596.0 49 57003.0 50 56930.5 51 55838.5 52 54932.0 53 53727.5 54 51414.5 55 48104.5 56 43827.0 57 39097.5 58 34707.5 59 30532.0 60 26017.5 61 21605.5 62 17248.0 63 12806.5 64 9222.0 65 6842.0 66 5112.0 67 3827.0 68 2817.0 69 2115.0 70 1587.0 71 1113.0 72 807.5 73 653.0 74 486.0 75 350.0 76 253.5 77 177.0 78 111.0 79 88.0 80 79.0 81 49.0 82 38.0 83 29.5 84 20.5 85 19.5 86 20.0 87 15.5 88 10.0 89 10.0 90 7.5 91 4.5 92 5.0 93 5.0 94 4.0 95 4.0 96 3.0 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5529763723962797E-4 48-49 3.7271432937510717E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1609812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.785373099311514 #Duplication Level Percentage of deduplicated Percentage of total 1 66.81131359576024 33.26226174620028 2 16.15897370554684 16.089610696652276 3 6.723444995063184 10.041876527757578 4 3.38651321417656 6.743952954941148 5 2.009698782023681 5.002680184014045 6 1.2582215124701772 3.758461646394469 7 0.8140811699003079 2.8370534343627587 8 0.557092020939254 2.2188027290488184 9 0.4396136485747525 1.96976965624593 >10 1.7679495378668777 14.26070577786007 >50 0.0537063007785621 1.8244722649962404 >100 0.018887891571059898 1.4673259533957308 >500 1.259063321490179E-4 0.03334732259893698 >1k 3.777189964470537E-4 0.4896791055317589 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3657 0.22716938375412782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2578 0.1601429235215044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.211905489585119E-5 0.0 3 0.0 0.0 0.0 6.211905489585119E-5 0.0 4 0.0 0.0 0.0 6.211905489585119E-5 0.0 5 0.0 0.0 0.0 6.211905489585119E-5 0.0 6 0.0 0.0 0.0 6.211905489585119E-5 0.0 7 0.0 0.0 0.0 6.211905489585119E-5 0.0 8 0.0 0.0 0.0 6.211905489585119E-5 0.0 9 0.0 0.0 0.0 6.211905489585119E-5 0.0 10-11 0.0 0.0 0.0 9.317858234377679E-5 0.0 12-13 0.0 0.0 0.0 2.1741669213547917E-4 0.0 14-15 3.1059527447925595E-5 0.0 0.0 2.4847621958340476E-4 0.0 16-17 6.211905489585119E-5 0.0 0.0 2.4847621958340476E-4 0.0 18-19 6.211905489585119E-5 0.0 0.0 2.4847621958340476E-4 0.0 20-21 6.211905489585119E-5 0.0 0.0 5.280119666147352E-4 0.0 22-23 6.211905489585119E-5 0.0 0.0 0.0013666192077087264 6.211905489585119E-5 24-25 6.211905489585119E-5 0.0 0.0 0.002391583613490271 6.211905489585119E-5 26-27 6.211905489585119E-5 0.0 0.0 0.003789262348646923 6.211905489585119E-5 28-29 6.211905489585119E-5 0.0 0.0 0.009411036816721455 6.211905489585119E-5 30-31 6.211905489585119E-5 0.0 0.0 0.026649074550320163 6.211905489585119E-5 32-33 6.211905489585119E-5 0.0 0.0 0.0652871266955396 6.211905489585119E-5 34-35 6.211905489585119E-5 0.0 0.0 0.12392751451722314 6.211905489585119E-5 36-37 6.211905489585119E-5 0.0 0.0 0.2239081333720956 1.2423810979170238E-4 38-39 6.211905489585119E-5 0.0 0.0 0.37535438920818087 1.2423810979170238E-4 40-41 6.211905489585119E-5 0.0 0.0 0.5362116818609874 1.2423810979170238E-4 42-43 6.211905489585119E-5 0.0 0.0 0.6889313783224376 1.2423810979170238E-4 44-45 9.317858234377679E-5 0.0 0.0 0.8697599471242605 1.2423810979170238E-4 46-47 1.2423810979170238E-4 0.0 0.0 1.0943203305727627 1.2423810979170238E-4 48-49 1.2423810979170238E-4 0.0 0.0 1.3300621439025178 1.2423810979170238E-4 50-51 1.2423810979170238E-4 0.0 0.0 1.5626358854325848 1.2423810979170238E-4 52-53 1.2423810979170238E-4 0.0 0.0 1.7921036742178589 1.2423810979170238E-4 54-55 1.2423810979170238E-4 0.0 0.0 2.0294916425023546 1.2423810979170238E-4 56-57 1.2423810979170238E-4 0.0 0.0 2.3099281158296745 1.2423810979170238E-4 58-59 1.2423810979170238E-4 0.0 0.0 2.6105532819981465 1.2423810979170238E-4 60-61 1.2423810979170238E-4 0.0 0.0 2.8741554914486906 1.2423810979170238E-4 62-63 1.2423810979170238E-4 0.0 0.0 3.1298685809274622 1.2423810979170238E-4 64-65 1.2423810979170238E-4 0.0 0.0 3.443942522480886 1.2423810979170238E-4 66-67 1.2423810979170238E-4 6.211905489585119E-5 0.0 3.817184863822608 1.2423810979170238E-4 68-69 1.2423810979170238E-4 6.211905489585119E-5 0.0 4.2379793416871046 1.2423810979170238E-4 70-71 1.2423810979170238E-4 6.211905489585119E-5 0.0 4.65666177168514 1.2423810979170238E-4 72-73 1.2423810979170238E-4 6.211905489585119E-5 0.0 5.076369166088959 1.2423810979170238E-4 74-75 1.2423810979170238E-4 6.211905489585119E-5 0.0 5.503934620937104 1.2423810979170238E-4 76-77 1.2423810979170238E-4 6.211905489585119E-5 0.0 5.9281145872934236 1.2423810979170238E-4 78-79 1.8635716468755358E-4 6.211905489585119E-5 0.0 6.406058595662103 1.2423810979170238E-4 80-81 1.8635716468755358E-4 6.211905489585119E-5 0.0 6.908508571187195 1.2423810979170238E-4 82-83 1.8635716468755358E-4 6.211905489585119E-5 0.0 7.404218629256087 1.2423810979170238E-4 84-85 1.8635716468755358E-4 6.211905489585119E-5 0.0 7.917756856080089 1.2423810979170238E-4 86-87 1.8635716468755358E-4 6.211905489585119E-5 0.0 8.459403955244463 1.2423810979170238E-4 88-89 1.8635716468755358E-4 6.211905489585119E-5 0.0 9.064474609457502 1.2423810979170238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2330 0.0 51.169525 1 GTATCAA 4275 0.0 44.555557 1 TCAACGC 5110 0.0 36.43836 4 CAACGCA 5175 0.0 36.072464 5 ATCAACG 5210 0.0 35.738964 3 AACGCAG 5275 0.0 35.298576 6 TATCAAC 5575 0.0 33.73991 2 ACGCAGA 5925 0.0 31.345991 7 CGCAGAG 6000 0.0 30.954166 8 GTACATG 5125 0.0 27.897562 1 TACATGG 5175 0.0 26.985506 2 GCAGAGT 7025 0.0 25.964413 9 ACATGGG 5300 0.0 25.542454 3 ACCGTAC 120 1.7112304E-4 23.75 8 AGAGTAC 6040 0.0 22.413078 10-11 GAGTACT 3875 0.0 22.064516 12-13 CAGAGTA 6580 0.0 21.656534 10-11 CATGGGG 3385 0.0 21.329395 4 CATGGGA 2110 0.0 20.710901 4 AGTACTT 4475 0.0 18.681566 12-13 >>END_MODULE