FastQCFastQC Report
Fri 10 Feb 2017
SRR2031494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609812
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47650.295997296578731No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT43430.2697830554126817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.21629854914735386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.1277788959207659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.055.5665131
GTATCAA44100.046.366011
TCAACGC54600.037.23364
ATCAACG54600.037.1466063
CAACGCA55550.036.4258355
AACGCAG56450.035.9292266
TATCAAC58300.034.9520532
ACGCAGA63750.031.6659647
CGCAGAG65200.030.8160328
GCAGAGT74700.026.5154729
GTACATG48250.024.7369561
TACATGG47900.024.394032
ACATGGG48300.023.1102523
AGAGTAC66050.023.04831510-11
GAGTACT45400.022.49399412-13
CAGAGTA71250.021.53285410-11
AGTACTT51400.020.56131412-13
TCAACCG1200.00476492519.7912275
GTACTTT52250.019.36320714-15
TAGGGCG1250.00603294518.9995785
Produced by FastQC (version 0.11.5)