FastQCFastQC Report
Fri 10 Feb 2017
SRR2031492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481982
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.19464282980295586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.14182214053123673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.055.130521
GTATCAA48650.046.3771821
TCAACGC57300.037.9668434
CAACGCA59150.037.0202875
ATCAACG60150.036.404823
AACGCAG61750.035.769236
TATCAAC63450.034.661152
ACGCAGA69700.031.6212317
CGCAGAG71350.030.8234068
GCAGAGT85100.025.5640439
GTACATG59050.025.1778161
TACATGG59150.024.0109882
ACATGGG59300.023.149243
AGAGTAC74450.021.43720610-11
GAGTACT55300.020.74367112-13
CAGAGTA82100.020.56790510-11
CATGGGG39700.020.1007564
GTACTTT60900.018.60221714-15
CGCCGTA2700.018.47222394-95
AGTACTT63200.017.21123312-13
Produced by FastQC (version 0.11.5)