##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031492_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2481982 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17996746148844 34.0 31.0 34.0 30.0 34.0 2 32.340590705331465 34.0 31.0 34.0 30.0 34.0 3 32.44393553216744 34.0 31.0 34.0 30.0 34.0 4 35.67246378096215 37.0 35.0 37.0 35.0 37.0 5 35.6580043690889 37.0 35.0 37.0 35.0 37.0 6 35.781599141331405 37.0 37.0 37.0 35.0 37.0 7 35.80776371464418 37.0 37.0 37.0 35.0 37.0 8 35.855336984716246 37.0 37.0 37.0 35.0 37.0 9 37.67661570470696 39.0 39.0 39.0 35.0 39.0 10-11 37.66872422926516 39.0 38.5 39.0 35.0 39.0 12-13 37.64235598807727 39.0 39.0 39.0 35.0 39.0 14-15 39.155797463478784 41.0 39.0 41.0 36.0 41.0 16-17 39.16345001696225 41.0 39.0 41.0 36.0 41.0 18-19 39.11895170875534 41.0 39.5 41.0 36.0 41.0 20-21 39.080670004859016 41.0 39.0 41.0 36.0 41.0 22-23 39.0095556696221 41.0 39.0 41.0 36.0 41.0 24-25 38.960677595566764 41.0 39.0 41.0 35.5 41.0 26-27 38.857471971996574 41.0 39.0 41.0 35.0 41.0 28-29 38.75908487652207 41.0 39.0 41.0 35.0 41.0 30-31 38.60488613535473 40.0 38.5 41.0 34.5 41.0 32-33 38.5088588474856 40.0 38.0 41.0 34.0 41.0 34-35 38.3811808465976 40.0 38.0 41.0 34.0 41.0 36-37 38.253531048976186 40.0 38.0 41.0 34.0 41.0 38-39 38.11445369063918 40.0 38.0 41.0 33.0 41.0 40-41 37.99604529766936 40.0 38.0 41.0 33.0 41.0 42-43 37.85332911358745 40.0 38.0 41.0 33.0 41.0 44-45 38.02974538090929 40.0 38.0 41.0 33.0 41.0 46-47 37.91857777373083 40.0 38.0 41.0 33.0 41.0 48-49 37.802230636644424 40.0 38.0 41.0 33.0 41.0 50-51 36.764331087010305 39.0 36.5 40.0 31.0 40.5 52-53 36.83422281064085 39.5 37.0 40.0 31.5 40.5 54-55 37.617818743246325 40.0 38.0 41.0 32.0 41.0 56-57 37.66339361042908 40.0 37.0 41.0 32.0 41.0 58-59 37.48193419613841 40.0 37.0 41.0 32.0 41.0 60-61 37.31858188334968 40.0 36.5 41.0 32.0 41.0 62-63 37.072094600202576 39.5 36.0 41.0 31.0 41.0 64-65 36.802287043177586 39.0 35.5 41.0 31.0 41.0 66-67 36.51693162964115 39.0 35.0 41.0 31.0 41.0 68-69 36.21729952110853 38.5 35.0 41.0 30.5 41.0 70-71 35.862388204265784 37.0 35.0 40.0 30.0 41.0 72-73 35.500972811245205 37.0 35.0 39.5 30.0 41.0 74-75 35.11664266702982 36.5 35.0 39.0 29.0 41.0 76-77 34.73635989302098 36.0 35.0 39.0 29.0 40.5 78-79 34.32936479797194 35.5 34.5 37.5 29.0 39.5 80-81 33.97393816715834 35.0 34.0 37.0 28.5 39.0 82-83 33.65643586456308 35.0 34.0 37.0 28.0 39.0 84-85 33.346434825071256 35.0 34.0 36.0 27.5 37.5 86-87 33.074907070236605 35.0 34.0 36.0 27.0 37.0 88-89 32.84112576158893 35.0 34.0 35.5 26.5 37.0 90-91 32.649665468967946 35.0 34.0 35.0 26.0 36.0 92-93 32.4856108948413 35.0 34.0 35.0 26.0 36.0 94-95 32.34409919169438 35.0 33.5 35.0 25.0 36.0 96-97 32.20995075709655 35.0 33.0 35.0 25.0 36.0 98-99 32.07723988328682 35.0 33.0 35.0 25.0 35.0 100-101 30.546395380788418 34.0 30.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21097974390713858 1101 2 0.1897252907926017 1101 3 0.26044421401202555 1101 4 0.11294218155350677 1101 5 0.059296438850957145 1101 6 0.08012183069192247 1101 7 0.09413415732573327 1101 8 0.19917438182948644 1101 9 0.341780083026201 1101 10-11 0.5036116987186361 1101 12-13 0.6261270179525127 1101 14-15 0.5646197189697446 1101 16-17 0.5206472263490198 1101 18-19 0.6268403158304992 1101 20-21 0.6154136637313243 1101 22-23 0.5779157677792597 1101 24-25 0.5799383085602159 1101 26-27 0.6522742345907844 1101 28-29 0.6893730590603582 1101 30-31 0.6527361186858585 1101 32-33 0.6178436391315714 1101 34-35 0.5677726508971332 1101 36-37 0.6512188423208727 1101 38-39 0.7221578294994586 1101 40-41 0.693760131383911 1101 42-43 0.6980893502666063 1101 44-45 0.685852295850701 1101 46-47 0.6879976007122224 1101 48-49 0.5932771252055247 1101 50-51 0.7039303520608229 1101 52-53 0.782510247576937 1101 54-55 0.7226925814380181 1101 56-57 0.60906083003281 1101 58-59 0.6359732014014128 1101 60-61 0.6500331812311586 1101 62-63 0.5618772265839809 1101 64-65 0.5191124977708199 1101 66-67 0.5592818607504242 1101 68-69 0.6242442263285568 1101 70-71 0.5605165608411582 1101 72-73 0.46673601841252577 1101 74-75 0.47570666974070974 1101 76-77 0.4654444583033879 1101 78-79 0.586659514643074 1101 80-81 0.5947847760463958 1101 82-83 0.5149519005122798 1101 84-85 0.44315692354020797 1101 86-87 0.457898966711042 1101 88-89 0.5043500326352159 1101 90-91 0.41332894316879276 1101 92-93 0.48102911786951097 1101 94-95 0.492200125980645 1101 96-97 0.5454458334440133 1101 98-99 0.6877917104575886 1101 100-101 0.703558822549418 1102 1 0.19744460449661716 1102 2 0.26412894879897664 1102 3 0.31379075435645376 1102 4 0.21769105592805715 1102 5 0.2046237115335714 1102 6 0.1942693398234283 1102 7 0.18151904631069016 1102 8 0.29877227624054115 1102 9 0.44935253940744246 1102 10-11 0.5012855289401799 1102 12-13 0.47152822697127306 1102 14-15 0.5943907619633535 1102 16-17 0.5631738225510006 1102 18-19 0.6755827226178965 1102 20-21 0.6538964827445071 1102 22-23 0.5540639303016377 1102 24-25 0.5016057080399605 1102 26-27 0.58192632851204 1102 28-29 0.5927510407703238 1102 30-31 0.6776610769892031 1102 32-33 0.6243342546208694 1102 34-35 0.6897510251328995 1102 36-37 0.7105868340282058 1102 38-39 0.6346145055084591 1102 40-41 0.581589989381925 1102 42-43 0.5924270182506959 1102 44-45 0.6088983255185809 1102 46-47 0.573530151122803 1102 48-49 0.5093925444933305 1102 50-51 0.5433091885829242 1102 52-53 0.6204303152741275 1102 54-55 0.5818975938794537 1102 56-57 0.4784750749364832 1102 58-59 0.5492816190901237 1102 60-61 0.6019036354360736 1102 62-63 0.6130575673789025 1102 64-65 0.6032485479984047 1102 66-67 0.6272940786685623 1102 68-69 0.5120071486069762 1102 70-71 0.5810946889499817 1102 72-73 0.5381349381068645 1102 74-75 0.4093629320992562 1102 76-77 0.4535077930040998 1102 78-79 0.46790940717746565 1102 80-81 0.35069292795731855 1102 82-83 0.2935162582855426 1102 84-85 0.4491866651069216 1102 86-87 0.4979259129438489 1102 88-89 0.5701644254115621 1102 90-91 0.5125227669055477 1102 92-93 0.3854796063160322 1102 94-95 0.4278601613030517 1102 96-97 0.5329205636746437 1102 98-99 0.5793794871354692 1102 100-101 0.43426536898560997 1103 1 0.28416382317003297 1103 2 0.383670669187282 1103 3 0.39870359052782334 1103 4 0.14341720685706605 1103 5 0.3101574981811268 1103 6 0.23229560791383363 1103 7 0.3274846436879386 1103 8 0.33748004880400373 1103 9 0.3796956605891282 1103 10-11 0.3702869418997423 1103 12-13 0.4024731047332111 1103 14-15 0.4520734143114993 1103 16-17 0.32587018164550585 1103 18-19 0.44553663451787173 1103 20-21 0.4292009218707804 1103 22-23 0.4248766713690131 1103 24-25 0.5314551588551666 1103 26-27 0.5004578594688098 1103 28-29 0.4651542886485345 1103 30-31 0.5609587525050443 1103 32-33 0.515594973603136 1103 34-35 0.5056173444202159 1103 36-37 0.522714399004812 1103 38-39 0.5166759032256678 1103 40-41 0.5775719319861992 1103 42-43 0.5240580988111532 1103 44-45 0.5814201690227989 1103 46-47 0.5515850147056938 1103 48-49 0.5840877591746576 1103 50-51 0.5861952561685797 1103 52-53 0.5814652475712592 1103 54-55 0.5515580335339152 1103 56-57 0.5453601890093509 1103 58-59 0.5818656189674698 1103 60-61 0.7415461522152782 1103 62-63 0.7062291942408621 1103 64-65 0.6390114596149914 1103 66-67 0.6545893713915447 1103 68-69 0.7034148262642645 1103 70-71 0.6422723267011818 1103 72-73 0.6477217723108666 1103 74-75 0.6565231652746775 1103 76-77 0.6320196800148139 1103 78-79 0.662289888454147 1103 80-81 0.7020244333947687 1103 82-83 0.7456675226049256 1103 84-85 0.6462362898096288 1103 86-87 0.6138640176987025 1103 88-89 0.6745097829961679 1103 90-91 0.6710032196460816 1103 92-93 0.5263564226515669 1103 94-95 0.640313122410312 1103 96-97 0.5516244737200111 1103 98-99 0.5271960516506482 1103 100-101 0.5616246083910106 1104 1 0.21922862524509412 1104 2 0.19367645382237697 1104 3 0.32751118223499276 1104 4 0.21424074934144954 1104 5 0.1978079014170362 1104 6 0.08820568570682497 1104 7 0.1432643874427697 1104 8 0.22163635814144556 1104 9 0.38573281515871827 1104 10-11 0.32692396639674115 1104 12-13 0.40202776408792573 1104 14-15 0.46442736861614975 1104 16-17 0.41625712035342843 1104 18-19 0.4424127136900253 1104 20-21 0.4694447698405426 1104 22-23 0.4495209598861294 1104 24-25 0.484918586561939 1104 26-27 0.5443771839628724 1104 28-29 0.5553584285299422 1104 30-31 0.5592959150271426 1104 32-33 0.4654901202833983 1104 34-35 0.5480852810920851 1104 36-37 0.626885981521923 1104 38-39 0.6557290532372733 1104 40-41 0.6261212522643405 1104 42-43 0.5184258686981522 1104 44-45 0.5602448096074042 1104 46-47 0.6843361806201358 1104 48-49 0.7131387195221279 1104 50-51 0.5727089949492736 1104 52-53 0.6413341509634947 1104 54-55 0.7029011909424483 1104 56-57 0.620184280543846 1104 58-59 0.7008123142631817 1104 60-61 0.7143408281722259 1104 62-63 0.7067650627912911 1104 64-65 0.7710231508963119 1104 66-67 0.7148002327792753 1104 68-69 0.6247936828238281 1104 70-71 0.5363832195721372 1104 72-73 0.5535336420334787 1104 74-75 0.618503406007008 1104 76-77 0.48627781418130667 1104 78-79 0.5527877265693419 1104 80-81 0.6108137016438775 1104 82-83 0.5631011708795057 1104 84-85 0.6714840976943819 1104 86-87 0.6602399056837669 1104 88-89 0.5883603346063353 1104 90-91 0.5491661676965762 1104 92-93 0.5530061602731564 1104 94-95 0.5361836990981246 1104 96-97 0.41418132600701085 1104 98-99 0.5342445913843292 1104 100-101 0.45378985940168803 1105 1 0.34959933905564355 1105 2 0.28596685638652986 1105 3 0.299451634187335 1105 4 0.2572971728300715 1105 5 0.24611459200505692 1105 6 0.2690430675279458 1105 7 0.2975772868319666 1105 8 0.16587608237391294 1105 9 0.36295175603400054 1105 10-11 0.33962529886998283 1105 12-13 0.385398558532259 1105 14-15 0.42917629517894085 1105 16-17 0.40110200111622163 1105 18-19 0.382309804826356 1105 20-21 0.4078158666615579 1105 22-23 0.48030945747719045 1105 24-25 0.4569573109355076 1105 26-27 0.40486814290012063 1105 28-29 0.4804183999049556 1105 30-31 0.45103347222673307 1105 32-33 0.4232143325921456 1105 34-35 0.46539735398501847 1105 36-37 0.47700290050513416 1105 38-39 0.5307456839547129 1105 40-41 0.4290296119716359 1105 42-43 0.5044112135083623 1105 44-45 0.4950408061401603 1105 46-47 0.4390880301574924 1105 48-49 0.33505397341775023 1105 50-51 0.4116747659090194 1105 52-53 0.3783324576540892 1105 54-55 0.39940521255230266 1105 56-57 0.4733804020589574 1105 58-59 0.45383154542007276 1105 60-61 0.37772956363293986 1105 62-63 0.4975340066903371 1105 64-65 0.43076774339334634 1105 66-67 0.44455712192797847 1105 68-69 0.36633217537528395 1105 70-71 0.376478871395463 1105 72-73 0.3373700965661044 1105 74-75 0.36770160384198647 1105 76-77 0.29038560138875624 1105 78-79 0.3304511611652643 1105 80-81 0.2997734250700006 1105 82-83 0.29530630321969653 1105 84-85 0.3266260131498697 1105 86-87 0.314352732502563 1105 88-89 0.37416274152471374 1105 90-91 0.2908251069591756 1105 92-93 0.2885218133059908 1105 94-95 0.29657235392506465 1105 96-97 0.29902822261433926 1105 98-99 0.33007935260754806 1105 100-101 0.34707457728424274 1106 1 0.27413397439284637 1106 2 0.1958119891298935 1106 3 0.11078822253033138 1106 4 0.15136568061157618 1106 5 0.23044477204778246 1106 6 0.19935661650566772 1106 7 0.1546264171340681 1106 8 0.10849127920027968 1106 9 0.21570198490186243 1106 10-11 0.18019403551935653 1106 12-13 0.1816680123009462 1106 14-15 0.10066126618900029 1106 16-17 0.21891745732617096 1106 18-19 0.19475640232406732 1106 20-21 0.2513236481690413 1106 22-23 0.22440657374207262 1106 24-25 0.23454351783206562 1106 26-27 0.3002869710966394 1106 28-29 0.23642236694797703 1106 30-31 0.20941766508605753 1106 32-33 0.16271883366020035 1106 34-35 0.1453542505367409 1106 36-37 0.2286965227205684 1106 38-39 0.25040543372584523 1106 40-41 0.3099515680290068 1106 42-43 0.24742692910189135 1106 44-45 0.2665105192924315 1106 46-47 0.2777705141367477 1106 48-49 0.25038049157460307 1106 50-51 0.11821847967160437 1106 52-53 0.0727633395582643 1106 54-55 0.1430197990643549 1106 56-57 0.1510390837800415 1106 58-59 0.26021505167580017 1106 60-61 0.25697887702933997 1106 62-63 0.20477386021490673 1106 64-65 0.12754070646932547 1106 66-67 0.19576058546170572 1106 68-69 0.15524543445371108 1106 70-71 0.19712275299448834 1106 72-73 0.20652633702994194 1106 74-75 0.21020427396710062 1106 76-77 0.2819041396859774 1106 78-79 0.29854316604777154 1106 80-81 0.22167989440170288 1106 82-83 0.2615292205221138 1106 84-85 0.19930300430031167 1106 86-87 0.2552456986301195 1106 88-89 0.2267832786013031 1106 90-91 0.31551994980323883 1106 92-93 0.12643568269872674 1106 94-95 0.29697477992446153 1106 96-97 0.24685207064608505 1106 98-99 0.17470095773418848 1106 100-101 0.2851753554349905 1107 1 0.20067555233988799 1107 2 0.21267377943242138 1107 3 0.2418127020021572 1107 4 0.1807365810502901 1107 5 0.24024942214710165 1107 6 0.22059091491799876 1107 7 0.20619836220173937 1107 8 0.2665853672577825 1107 9 0.25653665200677267 1107 10-11 0.27441794123124197 1107 12-13 0.2562668090484479 1107 14-15 0.33859614121937653 1107 16-17 0.25531718509386536 1107 18-19 0.27984775376811655 1107 20-21 0.30171020375158975 1107 22-23 0.34422513912784325 1107 24-25 0.27288147173120336 1107 26-27 0.24457920282992518 1107 28-29 0.3720344251956931 1107 30-31 0.3327267523575941 1107 32-33 0.34143175004701476 1107 34-35 0.22660746587892788 1107 36-37 0.2699865180047425 1107 38-39 0.310669877757455 1107 40-41 0.3527487549618513 1107 42-43 0.37117705414297575 1107 44-45 0.32275105189599884 1107 46-47 0.43903439369717034 1107 48-49 0.41434186459716926 1107 50-51 0.3769721594521016 1107 52-53 0.25716714366533466 1107 54-55 0.31071847148529486 1107 56-57 0.3709679532657333 1107 58-59 0.30549537592519016 1107 60-61 0.3011294550764134 1107 62-63 0.42331352324704596 1107 64-65 0.5467888165626391 1107 66-67 0.45771281619465753 1107 68-69 0.4189993285903526 1107 70-71 0.4871934190704863 1107 72-73 0.47131262395278384 1107 74-75 0.4847533222258136 1107 76-77 0.5214770529289083 1107 78-79 0.40104059377403445 1107 80-81 0.34572190106104017 1107 82-83 0.3857019844302485 1107 84-85 0.39008729771099837 1107 86-87 0.4691073067299243 1107 88-89 0.4452166567112599 1107 90-91 0.5494532421707348 1107 92-93 0.5326156596911744 1107 94-95 0.4834997274071995 1107 96-97 0.5153568114894966 1107 98-99 0.5744873161090851 1107 100-101 0.6660568414946972 1108 1 0.19730063873367953 1108 2 0.14727394785106185 1108 3 0.16378784574583705 1108 4 -0.06791923126271371 1108 5 0.19930702242805864 1108 6 0.21059229520617606 1108 7 0.22761027122079014 1108 8 0.24559408554700468 1108 9 0.4102018034871051 1108 10-11 0.34503611746301033 1108 12-13 0.3717962585784562 1108 14-15 0.41969176131173924 1108 16-17 0.36176247679336626 1108 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-0.4532715254942623 2316 96-97 -0.482827732309584 2316 98-99 -0.3705884511328037 2316 100-101 -0.4859453342201796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 32.0 7 540.0 8 1200.0 9 1132.0 10 1286.0 11 1908.0 12 2767.0 13 3371.0 14 3885.0 15 4412.0 16 4865.0 17 5785.0 18 7266.0 19 8686.0 20 10447.0 21 13058.0 22 15275.0 23 15933.0 24 15717.0 25 15098.0 26 15623.0 27 17154.0 28 19545.0 29 22925.0 30 27611.0 31 34062.0 32 42747.0 33 57073.0 34 82187.0 35 129485.0 36 241068.0 37 526465.0 38 822745.0 39 310297.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62935831122063 19.82371346770444 13.800583565875982 23.74634465519895 2 14.437493906079899 21.295319627620184 41.35046104282787 22.916725423472048 3 17.019301509841732 27.332833195405932 31.272184890946026 24.375680403806314 4 11.581590841512952 18.33103543861317 41.007348159656274 29.0800255602176 5 12.374747278586225 38.738999718773144 36.37653294826473 12.509720054375897 6 28.457176562924307 39.71531622711204 19.474033252457108 12.35347395750654 7 25.436848454178957 33.662129701182366 24.26351198356797 16.63750986107071 8 24.485914885764686 36.289989210236016 21.738030332210307 17.486065571789 9 25.865497815858458 16.343551242515055 21.186696760895124 36.60425418073137 10-11 23.15609057599934 26.77315951525837 30.758140067091546 19.312609841650747 12-13 24.54024646431763 24.66601288808702 29.869616298587175 20.92412434900817 14-15 21.88291454168483 25.992331934719914 27.324573667335216 24.800179856260037 16-17 21.412524345462618 29.085263309725857 27.779452066936827 21.7227602778747 18-19 21.334361006647107 28.45429580069477 29.354685086354372 20.85665810630375 20-21 22.303566262769028 27.57528056206693 29.165501603154254 20.95565157200979 22-23 21.983620348576256 27.57270197769363 28.754459137898664 21.689218535831444 24-25 21.677554470580368 27.73466930864124 28.958167303388986 21.629608917389408 26-27 21.685552111175667 28.0303604135727 28.760784727689405 21.52330274756223 28-29 21.729106012855855 27.888155514423552 28.892977467201614 21.489761005518975 30-31 22.021614177701533 27.715289635460692 28.8523446181318 21.410751568705976 32-33 21.761539769426207 27.89488400802262 28.766445526196406 21.577130696354768 34-35 21.943914178265594 27.925524842645917 28.71596167901298 21.414599300075505 36-37 22.034164631330928 27.74488292018234 28.614470209695313 21.606482238791415 38-39 21.825782781664007 28.057234097588136 28.672145889857383 21.444837230890474 40-41 21.898386047924603 27.848711231588304 28.8197295548477 21.433173165639396 42-43 21.823909278955288 27.769601068823224 28.65016748711312 21.75632216510837 44-45 21.876085322133683 28.00030378947148 28.462797071050478 21.660813817344366 46-47 21.9425462619655 27.862297009302456 28.387583378508968 21.807573350223077 48-49 21.801757591177836 28.0003859827037 28.37857248044049 21.81928394567797 50-51 21.803099297255176 28.10098945117249 28.155965675818763 21.939945575753573 52-53 21.91605337991976 28.07320923358832 28.09123917901097 21.919498207480956 54-55 21.883619623349404 28.001653517229375 28.17437837985932 21.940348479561898 56-57 21.81585120278874 28.09510705557091 28.152682009780893 21.936359731859458 58-59 21.808679515000513 27.99007003273996 28.258766582513488 21.94248386974603 60-61 21.933942309009495 28.051774750985302 28.207779105569664 21.806503834435546 62-63 21.81788586702079 27.954533916845488 28.28519707233977 21.94238314379395 64-65 21.81250710117962 27.885435913717345 28.230281283264745 22.071775701838288 66-67 21.82550074899818 27.97077094032108 28.08491358922023 22.11881472146051 68-69 21.77473486914893 27.963075477582027 28.07189979621125 22.19028985705779 70-71 21.905477154951164 27.773146622336505 28.099297255177515 22.222078967534816 72-73 21.8624470282218 27.824194534851582 28.021355513456587 22.292002923470033 74-75 21.894377155031744 27.848711231588304 28.013438453622953 22.243473159757002 76-77 21.855053743338992 27.90098800071878 27.96776930694904 22.276188948993184 78-79 21.86832942382338 27.866056240536796 27.88122556892032 22.3843887667195 80-81 21.919518352671375 28.032777836422667 27.725724844096373 22.321978966809592 82-83 21.94339040331477 27.94871195681516 27.865431739633888 22.242465900236184 84-85 22.06659838790128 27.84065315542176 27.79675678550449 22.295991671172473 86-87 21.93984484980149 27.852498527386583 27.91656023291063 22.291096389901295 88-89 22.087488950363056 27.747944989125628 27.838396894095123 22.326169166416197 90-91 22.14582942180886 27.807151703759338 27.85854208451149 22.188476789920315 92-93 22.316116716398426 27.69401631438101 27.745326114371498 22.244540854849067 94-95 22.201611454071788 27.550038638475215 27.84524625883669 22.40310364861631 96-97 22.201893486737614 27.591235552876693 27.86337693021142 22.34349403017427 98-99 22.240108912957467 27.505497622464624 27.751772575304734 22.50262088927317 100-101 22.562613266333116 27.333759874165082 27.727417040091346 22.376209819410455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 46.0 2 66.0 3 108.0 4 99.0 5 103.5 6 177.5 7 364.0 8 711.0 9 971.5 10 960.0 11 892.0 12 946.0 13 1093.5 14 1270.5 15 1436.5 16 1597.5 17 1748.5 18 1918.0 19 2236.0 20 2684.0 21 3165.5 22 3986.0 23 5254.5 24 6807.0 25 9013.5 26 12119.5 27 16503.0 28 21883.5 29 28223.5 30 35856.5 31 43845.0 32 52624.5 33 60983.0 34 67659.5 35 72452.5 36 77033.5 37 81142.5 38 82987.0 39 84651.0 40 87060.5 41 89072.5 42 89230.5 43 89905.0 44 91576.5 45 93164.5 46 93741.0 47 93927.0 48 94352.5 49 92406.5 50 88462.5 51 84640.5 52 81030.5 53 76535.0 54 71267.5 55 64283.5 56 57149.5 57 50846.5 58 44774.5 59 38701.0 60 31884.5 61 25136.5 62 19100.5 63 13927.0 64 9734.0 65 6777.5 66 4823.0 67 3453.0 68 2495.5 69 1873.0 70 1322.5 71 954.5 72 722.0 73 530.5 74 389.5 75 271.0 76 180.5 77 127.0 78 94.5 79 73.0 80 68.5 81 62.0 82 39.5 83 33.0 84 31.5 85 22.0 86 18.5 87 15.0 88 12.0 89 10.0 90 7.0 91 6.0 92 4.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.0072595208184427E-4 48-49 2.215970945800574E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2481982.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.843129715010946 #Duplication Level Percentage of deduplicated Percentage of total 1 67.43574120220457 34.96079878573799 2 16.2474603850743 16.846383925658174 3 6.720368873649277 10.452148657479643 4 3.345825392300376 6.93832239267223 5 1.898068963772593 4.920091774844948 6 1.1685137143967619 3.6347644841544344 7 0.7673157683648008 2.7846035638196756 8 0.5195251271214882 2.1547046844453495 9 0.37143733745664 1.7330826660086593 >10 1.4639230089273634 12.100660642093825 >50 0.045429118256788845 1.6120920689037403 >100 0.01599993569489796 1.3942926972405172 >500 1.5646911207828503E-4 0.046886484178950985 >1k 2.3470366811665288E-4 0.4211671727617906 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4831 0.19464282980295586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3520 0.14182214053123673 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.058076166547542E-5 2 0.0 0.0 0.0 0.0 8.058076166547542E-5 3 0.0 0.0 0.0 0.0 8.058076166547542E-5 4 0.0 0.0 0.0 0.0 8.058076166547542E-5 5 0.0 0.0 0.0 0.0 8.058076166547542E-5 6 0.0 0.0 0.0 0.0 1.2087114249821313E-4 7 0.0 0.0 0.0 0.0 1.2087114249821313E-4 8 0.0 0.0 0.0 0.0 1.2087114249821313E-4 9 0.0 0.0 0.0 0.0 1.2087114249821313E-4 10-11 0.0 0.0 0.0 6.0435571249106564E-5 1.2087114249821313E-4 12-13 0.0 0.0 0.0 1.2087114249821313E-4 1.2087114249821313E-4 14-15 0.0 0.0 0.0 2.0145190416368853E-4 1.2087114249821313E-4 16-17 0.0 0.0 0.0 3.223230466619017E-4 1.6116152333095084E-4 18-19 0.0 0.0 0.0 4.8348456999285246E-4 1.6116152333095084E-4 20-21 0.0 0.0 0.0 6.245009029074344E-4 1.6116152333095084E-4 22-23 0.0 0.0 0.0 0.0012490018058148688 1.6116152333095084E-4 24-25 0.0 0.0 0.0 0.0019943738512205166 1.6116152333095084E-4 26-27 0.0 0.0 0.0 0.0025181488020461066 1.6116152333095084E-4 28-29 0.0 0.0 0.0 0.005358620650754115 1.6116152333095084E-4 30-31 0.0 0.0 0.0 0.014947731288945689 1.6116152333095084E-4 32-33 0.0 0.0 0.0 0.037409618603196956 1.6116152333095084E-4 34-35 0.0 0.0 0.0 0.073348638305999 1.6116152333095084E-4 36-37 0.0 0.0 0.0 0.1362217775954862 1.6116152333095084E-4 38-39 0.0 0.0 0.0 0.2378341180556507 1.6116152333095084E-4 40-41 0.0 0.0 0.0 0.3458324838778041 1.6116152333095084E-4 42-43 0.0 0.0 0.0 0.4493586174275237 1.6116152333095084E-4 44-45 0.0 0.0 0.0 0.5701491791640713 2.2159709458005738E-4 46-47 0.0 0.0 0.0 0.7137038060711158 2.820326658291639E-4 48-49 0.0 0.0 0.0 0.8775043493466109 2.820326658291639E-4 50-51 0.0 0.0 0.0 1.0448705913258034 2.820326658291639E-4 52-53 0.0 0.0 0.0 1.2038161437109536 2.820326658291639E-4 54-55 0.0 0.0 0.0 1.3703765780734913 3.223230466619017E-4 56-57 0.0 0.0 0.0 1.5677792989634898 3.223230466619017E-4 58-59 0.0 0.0 0.0 1.782587464373231 3.223230466619017E-4 60-61 0.0 0.0 0.0 2.0080322903228147 3.223230466619017E-4 62-63 0.0 0.0 0.0 2.2306366444236905 4.0290380832737707E-4 64-65 0.0 0.0 0.0 2.45616205113494 4.4319418916011476E-4 66-67 0.0 0.0 0.0 2.7120059694228242 4.6333937957648364E-4 68-69 0.0 0.0 0.0 2.9967380907677814 4.834845699928525E-4 70-71 0.0 0.0 0.0 3.290535547800105 4.834845699928525E-4 72-73 0.0 0.0 0.0 3.5792765620379194 4.834845699928525E-4 74-75 0.0 0.0 0.0 3.8793794636705665 4.834845699928525E-4 76-77 0.0 0.0 0.0 4.188305958705582 4.834845699928525E-4 78-79 0.0 0.0 0.0 4.528900693075131 4.834845699928525E-4 80-81 0.0 0.0 0.0 4.890003231288542 4.834845699928525E-4 82-83 0.0 0.0 0.0 5.265005950889249 5.237749508255902E-4 84-85 0.0 0.0 0.0 5.6329377086538095 5.237749508255902E-4 86-87 0.0 0.0 0.0 6.0156560361839855 5.842105220746968E-4 88-89 0.0 0.0 0.0 6.452846958600022 6.446460933238034E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2490 0.0 55.13052 1 GTATCAA 4865 0.0 46.377182 1 TCAACGC 5730 0.0 37.966843 4 CAACGCA 5915 0.0 37.020287 5 ATCAACG 6015 0.0 36.40482 3 AACGCAG 6175 0.0 35.76923 6 TATCAAC 6345 0.0 34.66115 2 ACGCAGA 6970 0.0 31.621231 7 CGCAGAG 7135 0.0 30.823406 8 GCAGAGT 8510 0.0 25.564043 9 GTACATG 5905 0.0 25.177816 1 TACATGG 5915 0.0 24.010988 2 ACATGGG 5930 0.0 23.14924 3 AGAGTAC 7445 0.0 21.437206 10-11 GAGTACT 5530 0.0 20.743671 12-13 CAGAGTA 8210 0.0 20.567905 10-11 CATGGGG 3970 0.0 20.100756 4 GTACTTT 6090 0.0 18.602217 14-15 CGCCGTA 270 0.0 18.472223 94-95 AGTACTT 6320 0.0 17.211233 12-13 >>END_MODULE