FastQCFastQC Report
Fri 10 Feb 2017
SRR2031492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481982
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.234248274161537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.18255571555313457No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT33830.13630235835715168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.10604428235176563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.057.323391
GTATCAA52150.046.527341
TCAACGC60650.038.9232144
ATCAACG61000.038.6998833
CAACGCA62350.037.709595
AACGCAG63500.037.1014676
TATCAAC67600.035.4133452
ACGCAGA74000.031.8370677
CGCAGAG76100.031.0209318
GCAGAGT90050.026.0571169
GACCGTA1201.711876E-423.7494267
GTACATG58450.023.4423051
TACATGG57250.022.4841732
ACATGGG56800.022.1605553
AGAGTAC78450.022.16007810-11
CAGAGTA87000.021.07420310-11
GAGTACT61600.021.05062912-13
CATGGGG35600.018.8127464
GTACTTT69050.018.57304814-15
AGTACTT69900.018.2112912-13
Produced by FastQC (version 0.11.5)