FastQCFastQC Report
Fri 10 Feb 2017
SRR2031489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4291298
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.039.6215441
TCAACGC55950.035.317224
GTATCAA60150.035.0623131
CAACGCA57000.034.4999735
ATCAACG57550.034.335333
AACGCAG59250.033.350186
CGCAGAG65800.029.4528628
ACGCAGA67250.029.1709797
TATCAAC72000.028.1701132
TCGCCGG3850.024.0584294-95
GTACATG85600.024.0274311
ACATGGG82200.022.767623
GCAGAGT86450.021.7582239
TACATGG96050.020.5726012
AGAGTAC74950.019.77316510-11
GTCGCCG18750.019.5066594-95
CGCCGTA8900.019.4803294-95
TCGACCG757.726665E-418.99998334-35
TCGTACG650.00756704918.26921594-95
TATACAG42550.017.8613225
Produced by FastQC (version 0.11.5)