FastQCFastQC Report
Fri 10 Feb 2017
SRR2031489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4291298
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT86640.2018969551869854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.044.1290661
TCAACGC54600.035.7543534
ACCGTCG550.002580812434.5443538
ATCAACG56700.034.513893
GTATCAA60750.034.373271
CAACGCA56700.034.3463445
AACGCAG59100.032.87126
CGCAGAG66050.028.8370828
ACGCAGA67600.028.1758777
TATCAAC73400.027.3731042
ACATGGG71600.021.8254223
GTACATG76800.021.6775131
GCAGAGT85900.021.6756769
AGAGTAC76600.020.1527510-11
TACATGG83750.019.3963972
TATACAG44100.018.8486045
TACGCGG800.001192122317.81193212-13
CAGAGTA86050.017.63598610-11
GTATTCG2453.7165246E-617.4734651
GGACCGT2453.767691E-617.4484236
Produced by FastQC (version 0.11.5)