FastQCFastQC Report
Fri 10 Feb 2017
SRR2031487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239419
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.2715788607403953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.2154235169865881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.13893606601157477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.059.6455731
GTATCAA38650.043.7516171
TCAACGC44850.037.0680054
ATCAACG45650.036.730563
AACGCAG46600.036.083696
CAACGCA46350.035.868395
TATCAAC47500.035.42
CGCAGAG52000.031.9711558
ACGCAGA52450.031.7874187
GCAGAGT58300.027.4571179
GTACCGT700.0083918227.1428596
GTACATG39900.026.4285721
TACATGG39400.026.040612
ACATGGG39600.025.3093413
CAGAGTA54750.024.33561710-11
GAGTACT35900.023.81615612-13
GGACCGT1000.001653719123.7499986
CATGGGG24800.023.1754044
AGAGTAC51250.023.03170810-11
TGGACCG1100.002877492221.590915
Produced by FastQC (version 0.11.5)