##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031487_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1239419 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.960524245634446 34.0 31.0 34.0 30.0 34.0 2 32.12174333296488 34.0 31.0 34.0 30.0 34.0 3 32.22528297532957 34.0 31.0 34.0 30.0 34.0 4 35.46550278799986 37.0 35.0 37.0 33.0 37.0 5 35.44439773797239 37.0 35.0 37.0 33.0 37.0 6 35.578996287776775 37.0 36.0 37.0 33.0 37.0 7 35.5967489606017 37.0 36.0 37.0 33.0 37.0 8 35.648829007785096 37.0 36.0 37.0 35.0 37.0 9 37.44999390843613 39.0 38.0 39.0 35.0 39.0 10-11 37.43748240102822 39.0 38.0 39.0 35.0 39.0 12-13 37.40657961512612 39.0 38.0 39.0 35.0 39.0 14-15 38.87410794896641 41.0 39.0 41.0 36.0 41.0 16-17 38.874576716994014 41.0 39.0 41.0 36.0 41.0 18-19 38.846873010660644 41.0 39.0 41.0 35.0 41.0 20-21 38.81107680292137 41.0 39.0 41.0 35.0 41.0 22-23 38.73285224770639 41.0 39.0 41.0 35.0 41.0 24-25 38.675517319001884 41.0 39.0 41.0 34.5 41.0 26-27 38.562458700407205 41.0 39.0 41.0 34.0 41.0 28-29 38.4487949595738 40.0 38.5 41.0 34.0 41.0 30-31 38.27570498757886 40.0 38.0 41.0 34.0 41.0 32-33 38.14068083513324 40.0 38.0 41.0 33.5 41.0 34-35 37.99596625515665 40.0 38.0 41.0 33.0 41.0 36-37 37.848663365657615 40.0 38.0 41.0 33.0 41.0 38-39 37.693143319571504 40.0 38.0 41.0 32.5 41.0 40-41 37.55704285637061 40.0 38.0 41.0 32.0 41.0 42-43 37.38922027175636 40.0 37.0 41.0 31.0 41.0 44-45 37.53573851941918 40.0 38.0 41.0 32.0 41.0 46-47 37.38144082025529 40.0 38.0 41.0 31.0 41.0 48-49 37.22724074747926 40.0 37.5 41.0 30.5 41.0 50-51 36.17923922418488 39.0 36.0 40.0 29.0 40.5 52-53 36.25248362337514 39.0 36.5 40.0 29.5 40.5 54-55 37.0351935059895 40.0 37.0 41.0 30.0 41.0 56-57 37.06794756252728 40.0 37.0 41.0 30.0 41.0 58-59 36.880029675194585 40.0 36.5 41.0 30.0 41.0 60-61 36.715588110235515 40.0 36.0 41.0 29.5 41.0 62-63 36.465528203133886 39.0 35.0 41.0 29.0 41.0 64-65 36.20017040242243 39.0 35.0 41.0 29.0 41.0 66-67 35.90416840471221 39.0 35.0 41.0 28.0 41.0 68-69 35.595175239366185 37.5 35.0 40.5 28.0 41.0 70-71 35.22665458573735 37.0 35.0 40.0 28.0 41.0 72-73 34.863908815340096 37.0 35.0 39.0 27.0 41.0 74-75 34.4831759881041 36.0 34.5 39.0 26.0 41.0 76-77 34.10676978487501 36.0 34.0 39.0 26.0 40.5 78-79 33.70455592499388 35.0 34.0 37.0 26.0 39.5 80-81 33.35860512062507 35.0 34.0 37.0 26.0 39.0 82-83 33.04510944240809 35.0 34.0 36.5 25.0 39.0 84-85 32.747795136269495 35.0 34.0 36.0 25.0 37.0 86-87 32.476869807546926 35.0 33.5 36.0 24.5 37.0 88-89 32.24445808883033 35.0 33.0 35.0 24.0 37.0 90-91 32.050043609142676 35.0 33.0 35.0 23.5 36.0 92-93 31.87452507989631 35.0 33.0 35.0 23.0 36.0 94-95 31.730850906755506 35.0 33.0 35.0 23.0 36.0 96-97 31.593871402649143 35.0 33.0 35.0 20.5 36.0 98-99 31.457371155355858 35.0 33.0 35.0 20.0 35.0 100-101 29.864326349684813 33.5 28.5 34.5 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1974228039358188 1101 2 0.1912869070183163 1101 3 0.33005586278923005 1101 4 0.20435386459286775 1101 5 0.0942962178673028 1101 6 0.020074411662164948 1101 7 0.08779371507667832 1101 8 0.2146408939173341 1101 9 0.47336706231855885 1101 10-11 0.5003939263181536 1101 12-13 0.6735431995497407 1101 14-15 0.7964501217989124 1101 16-17 0.646783249946921 1101 18-19 0.7006936876785161 1101 20-21 0.718876783766909 1101 22-23 0.7789777338368182 1101 24-25 0.6518322771605298 1101 26-27 0.7218321511695365 1101 28-29 0.7268689563300939 1101 30-31 0.7890170820295239 1101 32-33 0.7175429505914934 1101 34-35 0.7990376313508349 1101 36-37 0.8775288763963474 1101 38-39 0.8473967699504286 1101 40-41 0.7679804738151574 1101 42-43 0.752410368808718 1101 44-45 0.8882814577555536 1101 46-47 1.152803654341902 1101 48-49 1.1063155671662628 1101 50-51 1.1014533219014169 1101 52-53 1.1175820114769763 1101 54-55 1.0929513206314994 1101 56-57 1.145675672715221 1101 58-59 1.0316694488073779 1101 60-61 0.944924425341803 1101 62-63 0.9192983756383413 1101 64-65 0.9119567522393481 1101 66-67 0.9518790229916121 1101 68-69 0.9999250767733798 1101 70-71 0.9541915758724926 1101 72-73 0.9440050547668903 1101 74-75 0.938737065633795 1101 76-77 0.9532944267077639 1101 78-79 0.9742568565889016 1101 80-81 0.7844601738295509 1101 82-83 0.8234334315501002 1101 84-85 0.8673976903301011 1101 86-87 0.8488979271267496 1101 88-89 0.762544041694234 1101 90-91 0.6976442879897533 1101 92-93 0.7367290859615316 1101 94-95 0.8031272534000315 1101 96-97 0.8048373671872007 1101 98-99 0.8679027466916942 1101 100-101 0.884010156174579 1102 1 0.12688519237610407 1102 2 0.2348536303502371 1102 3 0.2689902019307482 1102 4 0.11937192664132112 1102 5 0.09205439891277223 1102 6 0.15523697009820836 1102 7 0.1811426313537723 1102 8 0.24696664827347092 1102 9 0.4276700797431232 1102 10-11 0.39269365007505996 1102 12-13 0.3985883546708635 1102 14-15 0.5006070491257262 1102 16-17 0.4825806362622629 1102 18-19 0.5171195270325271 1102 20-21 0.6127834985990432 1102 22-23 0.663784363671077 1102 24-25 0.5828804072072771 1102 26-27 0.48396825401696475 1102 28-29 0.5309754161685944 1102 30-31 0.5627049273333924 1102 32-33 0.5387977317219566 1102 34-35 0.5023844226810539 1102 36-37 0.5608506996007705 1102 38-39 0.5262641732653464 1102 40-41 0.4932939034794188 1102 42-43 0.5853098588214678 1102 44-45 0.39432958780229654 1102 46-47 0.485960794163411 1102 48-49 0.5737082573490113 1102 50-51 0.5149095479957566 1102 52-53 0.5913070433511791 1102 54-55 0.6421757150216365 1102 56-57 0.5706942660003946 1102 58-59 0.6491391683143846 1102 60-61 0.6197172430213627 1102 62-63 0.6359978019026826 1102 64-65 0.7752057960132532 1102 66-67 0.7105249005946703 1102 68-69 0.4916457525064857 1102 70-71 0.4932215376234481 1102 72-73 0.40548242408266333 1102 74-75 0.36447282848972407 1102 76-77 0.31744391672051364 1102 78-79 0.38868790206276316 1102 80-81 0.3863248147135323 1102 82-83 0.3719990924085863 1102 84-85 0.34309095424850966 1102 86-87 0.46909554718624946 1102 88-89 0.39508229073801004 1102 90-91 0.3947517040543502 1102 92-93 0.5093650825616187 1102 94-95 0.38881042382076814 1102 96-97 0.4467386634047905 1102 98-99 0.4424910382344045 1102 100-101 0.27909887270666545 1103 1 0.3178277267229923 1103 2 0.28604705388046625 1103 3 0.4382950697736945 1103 4 0.30321232694817013 1103 5 0.21443566678361492 1103 6 0.20789667440115522 1103 7 0.35372440135168404 1103 8 0.31930171348025027 1103 9 0.21821160983587617 1103 10-11 0.3314799148076091 1103 12-13 0.3982958830898582 1103 14-15 0.3470158816802993 1103 16-17 0.3309805421134371 1103 18-19 0.41249926062622677 1103 20-21 0.37243288140118835 1103 22-23 0.32847158035284707 1103 24-25 0.3462342847440709 1103 26-27 0.3899297053036648 1103 28-29 0.285282724907006 1103 30-31 0.42361834528443154 1103 32-33 0.17945346085230085 1103 34-35 0.315361269044125 1103 36-37 0.4278101284312328 1103 38-39 0.3310465848700588 1103 40-41 0.4127173291018451 1103 42-43 0.32395707968763787 1103 44-45 0.3734359585394458 1103 46-47 0.384966565137681 1103 48-49 0.35913785494995665 1103 50-51 0.37793434051964425 1103 52-53 0.5232531344314495 1103 54-55 0.4661609999090075 1103 56-57 0.4695152263183999 1103 58-59 0.63576175357138 1103 60-61 0.5431873246170085 1103 62-63 0.5232925051595387 1103 64-65 0.780082356442847 1103 66-67 0.5738081665127979 1103 68-69 0.5424194291924493 1103 70-71 0.4579392654984744 1103 72-73 0.49747149235798815 1103 74-75 0.5427161683321131 1103 76-77 0.5092473025912199 1103 78-79 0.6358272597585568 1103 80-81 0.653675222691767 1103 82-83 0.53749703508241 1103 84-85 0.5165884959180786 1103 86-87 0.5927251587351705 1103 88-89 0.5055162470808554 1103 90-91 0.408694011746654 1103 92-93 0.41828917071281424 1103 94-95 0.5964443243717206 1103 96-97 0.5723623993696414 1103 98-99 0.5644019978550183 1103 100-101 0.2990074263017597 1104 1 0.32034138895729924 1104 2 0.3193888867604926 1104 3 0.42339725321279786 1104 4 0.34857865741054894 1104 5 0.28802127925465726 1104 6 0.2679239359101686 1104 7 0.3017756441742847 1104 8 0.3420254089572339 1104 9 0.44662040025593797 1104 10-11 0.5869644834083871 1104 12-13 0.5724732371494952 1104 14-15 0.6254067286129015 1104 16-17 0.5535742260058569 1104 18-19 0.7095540889983383 1104 20-21 0.7060794387699829 1104 22-23 0.7649996414488456 1104 24-25 0.7325398623354928 1104 26-27 0.6500473138460237 1104 28-29 0.7544529802711608 1104 30-31 0.7394410384445038 1104 32-33 0.7701145052262319 1104 34-35 0.7242126812280674 1104 36-37 0.8198784773785519 1104 38-39 0.7929308399320192 1104 40-41 0.754625367154631 1104 42-43 0.7369925511291555 1104 44-45 0.8251522373749509 1104 46-47 0.8683058796334961 1104 48-49 0.7855872637592611 1104 50-51 0.7529170266282392 1104 52-53 0.971128090359727 1104 54-55 0.9933709607481376 1104 56-57 0.7889034006157729 1104 58-59 0.9664535379725052 1104 60-61 0.9340401596880241 1104 62-63 0.8923613168599545 1104 64-65 0.9651229968679544 1104 66-67 0.8615398578596327 1104 68-69 0.9890950046432394 1104 70-71 0.6289119756576369 1104 72-73 0.5263758535698315 1104 74-75 0.6032014609683571 1104 76-77 0.6025467479170956 1104 78-79 0.6872522717208795 1104 80-81 0.6613248147135309 1104 82-83 0.5143802355709681 1104 84-85 0.5819397790348333 1104 86-87 0.7131460279554815 1104 88-89 0.6049046732166445 1104 90-91 0.8123398450799897 1104 92-93 0.6175047727325627 1104 94-95 0.6486795477523977 1104 96-97 0.6092586954560808 1104 98-99 0.7042565296873953 1104 100-101 0.7717537872365803 1105 1 0.3798226395443187 1105 2 0.3357095052445729 1105 3 0.41397118248865183 1105 4 0.3723199401662569 1105 5 0.3139690227504772 1105 6 0.21291871143379382 1105 7 0.2412018029514087 1105 8 0.3203922611221657 1105 9 0.5496269690077042 1105 10-11 0.5262365494833432 1105 12-13 0.5493332828196316 1105 14-15 0.549872687333675 1105 16-17 0.5756755221456089 1105 18-19 0.6269673714957591 1105 20-21 0.6201059838061411 1105 22-23 0.5717725293515485 1105 24-25 0.5296735179427046 1105 26-27 0.5376506293339602 1105 28-29 0.565601367140701 1105 30-31 0.5817983339267911 1105 32-33 0.5019528458386162 1105 34-35 0.6390496805001575 1105 36-37 0.6143217477833574 1105 38-39 0.6324983237302675 1105 40-41 0.4482072593543194 1105 42-43 0.5439848376887468 1105 44-45 0.5296053697126979 1105 46-47 0.528411132286827 1105 48-49 0.5793506918375968 1105 50-51 0.46060904081063825 1105 52-53 0.6090373899276855 1105 54-55 0.5180210235592568 1105 56-57 0.6794590588997949 1105 58-59 0.6894656687370428 1105 60-61 0.7123524923873745 1105 62-63 0.6368378272619424 1105 64-65 0.6266216878137598 1105 66-67 0.7138163207130148 1105 68-69 0.7432254228361543 1105 70-71 0.7512414023740774 1105 72-73 0.6427235483430138 1105 74-75 0.6736497093012233 1105 76-77 0.6842602734407208 1105 78-79 0.7074167693493223 1105 80-81 0.7353421435385528 1105 82-83 0.7268384837864375 1105 84-85 0.7529863473169769 1105 86-87 0.750012706949569 1105 88-89 0.6727344462747809 1105 90-91 0.7197675535894277 1105 92-93 0.815208277827896 1105 94-95 0.7402157492645607 1105 96-97 0.7479168121791027 1105 98-99 0.7484980120298381 1105 100-101 0.7840386444733625 1106 1 0.21627684647364376 1106 2 0.19673774296963842 1106 3 0.06790297216704744 1106 4 0.10414442538440483 1106 5 0.18416098513700518 1106 6 0.16916985094285053 1106 7 0.1272964775076204 1106 8 0.1881242651663868 1106 9 0.08004564535397662 1106 10-11 0.10131293112080897 1106 12-13 0.12065032048282376 1106 14-15 0.26322331358022666 1106 16-17 0.12669514103853174 1106 18-19 0.1751637703708937 1106 20-21 0.30973548452161737 1106 22-23 0.21419286040309515 1106 24-25 0.06942201102830126 1106 26-27 0.2411420850878514 1106 28-29 0.23739824170906587 1106 30-31 0.23035198615691854 1106 32-33 0.19775952156610543 1106 34-35 0.20447718286205685 1106 36-37 0.16097324862038676 1106 38-39 0.2610680948339734 1106 40-41 0.3350736923180335 1106 42-43 0.23423519818798155 1106 44-45 0.22343655110043414 1106 46-47 0.34655342865310246 1106 48-49 0.4578962915370468 1106 50-51 0.2358622880661443 1106 52-53 0.19452474068652492 1106 54-55 0.3785067859115401 1106 56-57 0.4167884087857132 1106 58-59 0.5299492487568216 1106 60-61 0.4736388116488115 1106 62-63 0.4858815374481864 1106 64-65 0.3476291243189351 1106 66-67 0.4967522761602865 1106 68-69 0.49333315823800916 1106 70-71 0.5463701047426 1106 72-73 0.5034745055306189 1106 74-75 0.4340838139095311 1106 76-77 0.5224558573942275 1106 78-79 0.6324881785836283 1106 80-81 0.540409782033791 1106 82-83 0.47079874047292947 1106 84-85 0.5296011352439862 1106 86-87 0.546351706060058 1106 88-89 0.3888354330205672 1106 90-91 0.5598378025963271 1106 92-93 0.5622811207717753 1106 94-95 0.36584662291579306 1106 96-97 0.47499092584823543 1106 98-99 0.4384783434128856 1106 100-101 0.17749316632155043 1107 1 0.3222285879008062 1107 2 0.2934563390838818 1107 3 0.2801869725251862 1107 4 0.22980473517041844 1107 5 0.3364463284980985 1107 6 0.34550613888268344 1107 7 0.23466956372840997 1107 8 0.41881859769391383 1107 9 0.4243244527673582 1107 10-11 0.3527007008405718 1107 12-13 0.2775410448090838 1107 14-15 0.3797735866574925 1107 16-17 0.3717484134890654 1107 18-19 0.38673150248656185 1107 20-21 0.35891177604278823 1107 22-23 0.4410204635904975 1107 24-25 0.45808147024578005 1107 26-27 0.4708627404733008 1107 28-29 0.5000504487105957 1107 30-31 0.3536900200005917 1107 32-33 0.4225166618651457 1107 34-35 0.5028756518386857 1107 36-37 0.5147538019375943 1107 38-39 0.4595639315248192 1107 40-41 0.5120311191243729 1107 42-43 0.6972690418624339 1107 44-45 0.4482776290841457 1107 46-47 0.5728885974238054 1107 48-49 0.41074569613906675 1107 50-51 0.48177172490024134 1107 52-53 0.4292928488430263 1107 54-55 0.5045308256644176 1107 56-57 0.5831752831701635 1107 58-59 0.45480263760094175 1107 60-61 0.4953115218287749 1107 62-63 0.5980278800034782 1107 64-65 0.645563138107093 1107 66-67 0.6225580779330429 1107 68-69 0.41040272579105874 1107 70-71 0.5215245500823826 1107 72-73 0.44913324847985336 1107 74-75 0.5125431656867718 1107 76-77 0.47841095632877284 1107 78-79 0.3212248604709913 1107 80-81 0.3590987873162703 1107 82-83 0.33099772874403754 1107 84-85 0.5118734962431759 1107 86-87 0.46116614781849563 1107 88-89 0.4621242234225704 1107 90-91 0.4562363163397194 1107 92-93 0.5419961803115747 1107 94-95 0.5286689436249041 1107 96-97 0.3961734399497665 1107 98-99 0.4345612514619468 1107 100-101 0.45642368830202784 1108 1 0.19857271537941656 1108 2 0.10539865550052241 1108 3 -0.10578436560697924 1108 4 -0.19708771021305438 1108 5 0.05861371678788174 1108 6 0.0677062990149011 1108 7 -0.008668434773078104 1108 8 0.09485801129641658 1108 9 0.07509677585402841 1108 10-11 0.0648726771882977 1108 12-13 0.12599986859552814 1108 14-15 0.015670384912745305 1108 16-17 0.040117288206808155 1108 18-19 0.1958583320871199 1108 20-21 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2316 64-65 -1.2209623723113125 2316 66-67 -0.7901518219718611 2316 68-69 -0.6542799154274732 2316 70-71 -0.9701312825421766 2316 72-73 -0.7614180628711438 2316 74-75 -0.6387538914727813 2316 76-77 -0.616395141141048 2316 78-79 -0.6284651759019226 2316 80-81 -0.5298143186649398 2316 82-83 -0.5510556964045037 2316 84-85 -0.5853974708335201 2316 86-87 -0.49737625115758277 2316 88-89 -0.535586033828416 2316 90-91 -0.39665466800961013 2316 92-93 -0.3470909613944251 2316 94-95 -0.4664321416706052 2316 96-97 -0.4562712709736232 2316 98-99 -0.4413799190074066 2316 100-101 -0.3852073483234868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 41.0 7 788.0 8 1713.0 9 1364.0 10 1377.0 11 1725.0 12 2119.0 13 2440.0 14 2518.0 15 2787.0 16 3125.0 17 3878.0 18 4707.0 19 5963.0 20 7498.0 21 9229.0 22 10214.0 23 10262.0 24 9428.0 25 8947.0 26 8884.0 27 9787.0 28 11094.0 29 12666.0 30 14920.0 31 18220.0 32 22861.0 33 30162.0 34 42670.0 35 66874.0 36 123322.0 37 260075.0 38 388792.0 39 138803.0 40 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10706790843129 20.6257125314361 14.169300293121212 23.0979192670114 2 14.566825262481856 21.868391560884575 41.01179665633656 22.55298652029701 3 16.73114580299318 27.79221554615509 32.04106117463102 23.43557747622071 4 11.67426027840464 18.554822864584132 40.76297039177227 29.007946465238955 5 12.171670758637717 39.13640181407579 36.21285457137578 12.479072855910713 6 28.035797417983748 40.02302691825766 19.225621036953605 12.715554626804979 7 24.714644522957936 34.464212667386896 24.399738909924732 16.42140389973044 8 24.461784110135476 36.005176619044896 21.967470242105374 17.56556902871426 9 26.14975242432139 15.843552503229336 21.533395889525657 36.47329918292361 10-11 23.0309120644431 27.134528355624692 30.621484744061533 19.213074835870678 12-13 24.266087578131366 25.40642026626992 29.774555658740105 20.55293649685861 14-15 21.792347866217963 26.61174308284769 27.67510422222025 23.920804828714097 16-17 20.87784679757209 29.51794348803754 28.1774363633283 21.42677335106207 18-19 20.929241846381245 28.579156846877446 30.176397166736997 20.315204140004308 20-21 21.983969908481313 27.959995772212626 29.742524521570186 20.313509797735875 22-23 21.728124225947802 27.85595508863427 29.389939963805624 21.025980721612303 24-25 21.34617106886372 28.026317169577037 29.583942153541297 21.04356960801795 26-27 21.22365398626292 28.217132382188755 29.412813584429482 21.146400047118853 28-29 21.214173737856203 28.177718753706372 29.666521168386158 20.941586340051266 30-31 21.675357566730863 28.09158968839432 29.359441802973812 20.873610941901003 32-33 21.413420320327507 28.26901152878889 29.272990005801102 21.044578145082493 34-35 21.535695354032818 28.33440507205392 29.284003230545924 20.845896343367336 36-37 21.62844042248828 28.077510510973287 29.194606505144748 21.099442561393687 38-39 21.35520756096203 28.349170054678847 29.281502058625858 21.01412032573327 40-41 21.48341279260686 28.227540484694845 29.27545083623859 21.013595886459704 42-43 21.373441911088985 28.205998132996186 29.188474599792325 21.232085356122507 44-45 21.534041353246966 28.296887493252886 29.002500365090416 21.16657078840973 46-47 21.566407082045092 28.230594086247073 28.751202179090797 21.451796652617038 48-49 21.373116096350333 28.42830223464386 28.778185183407167 21.420396485598644 50-51 21.308532465614938 28.581456311384606 28.53470053307235 21.575310689928102 52-53 21.378202206033635 28.547327417120442 28.511584863553 21.562885513292922 54-55 21.38227669577439 28.35614913116549 28.564714596113177 21.696859576946938 56-57 21.399784899214875 28.477294603358512 28.42360009004219 21.69932040738443 58-59 21.41140324619842 28.406495301427526 28.459625033987702 21.722476418386357 60-61 21.40228607113494 28.277725289026552 28.627970040801376 21.69201859903713 62-63 21.372715764402514 28.247025420781835 28.644550390142477 21.735708424673174 64-65 21.278558744056692 28.14387224982028 28.571976062977892 22.005592943145135 66-67 21.336771503422167 28.302535300814334 28.35618947264807 22.004503723115427 68-69 21.449162873894945 28.173603922483036 28.316049697479222 22.061183506142797 70-71 21.259436881312936 27.847967475083085 28.557856544074276 22.3347390995297 72-73 21.29679309418365 27.868824021577854 28.5120286198614 22.3223542643771 74-75 21.20328153755913 27.994407056854865 28.390842806185802 22.4114685994002 76-77 21.124252573181465 28.030674049695865 28.379627874028074 22.465445503094593 78-79 21.223775010710664 28.002031597062814 28.242144101389442 22.53204929083708 80-81 21.17024186332467 27.91969463111345 28.22350633643667 22.68655716912521 82-83 21.181618161412725 27.827352977483805 28.401412274622224 22.589616586481245 84-85 21.29344475112936 27.604304920289263 28.327183946671784 22.77506638190959 86-87 21.14063121510966 27.66772173090779 28.4198079906795 22.771839063303048 88-89 21.305748903316797 27.50756604505821 28.407624862939812 22.779060188685182 90-91 21.406279877910535 27.345635333975032 28.495367587555137 22.752717200559296 92-93 21.480992303651952 27.26140231834432 28.43049848356367 22.827106894440057 94-95 21.332818038129155 27.08974930995894 28.579116505394865 22.998316146517038 96-97 21.35097170529095 26.887436774811423 28.608444763231805 23.153146756665823 98-99 21.35908034328988 26.87912642939958 28.477536652254 23.28425657505654 100-101 21.55554336346304 26.740755144144153 28.542889854036446 23.16081163835636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 73.0 1 59.0 2 62.0 3 88.0 4 93.5 5 104.5 6 154.5 7 287.5 8 528.0 9 708.0 10 667.5 11 616.5 12 693.0 13 770.5 14 861.5 15 961.0 16 1030.5 17 1143.5 18 1315.0 19 1472.0 20 1673.5 21 2014.0 22 2505.5 23 3166.5 24 4013.0 25 5443.0 26 7793.5 27 10961.5 28 14473.0 29 18515.5 30 22349.0 31 25253.0 32 28160.0 33 31078.0 34 34270.0 35 36636.5 36 38668.0 37 40435.0 38 40414.0 39 40517.5 40 41631.5 41 42865.5 42 43449.5 43 44255.5 44 45156.5 45 45562.5 46 46124.0 47 46760.0 48 46736.0 49 45950.5 50 44568.5 51 42241.5 52 39208.5 53 36693.5 54 34322.0 55 30893.5 56 26851.5 57 23183.0 58 20340.0 59 17400.5 60 14055.0 61 10918.0 62 8275.5 63 6062.5 64 4355.5 65 3063.5 66 2158.5 67 1612.0 68 1198.5 69 868.5 70 654.0 71 506.5 72 374.5 73 256.5 74 185.0 75 146.5 76 116.0 77 82.5 78 61.5 79 49.0 80 39.0 81 35.0 82 33.0 83 27.5 84 17.5 85 18.5 86 18.0 87 14.5 88 8.5 89 4.0 90 4.5 91 3.5 92 4.0 93 3.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 2.420488954905484E-4 48-49 3.630733432358226E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1239419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.99573161489145 #Duplication Level Percentage of deduplicated Percentage of total 1 73.44198713250985 44.06205749266372 2 14.81217487550833 17.773345369276715 3 5.4091299571783 9.735741275428156 4 2.482729079402086 5.958125900811763 5 1.2968596317176226 3.890302120335872 6 0.7532038921715546 2.7113411139609735 7 0.4722884567147787 1.9834704045709795 8 0.30380300059965726 1.458150663022059 9 0.1999736181898062 1.0797807174276948 >10 0.7774482332884782 7.715242530860947 >50 0.03376265964427838 1.4099020533388702 >100 0.01609834669975063 1.5482491034302117 >500 1.3527909388486655E-4 0.044638925154021854 >1k 4.0583728165459964E-4 0.6296523297180028 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3366 0.2715788607403953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2670 0.2154235169865881 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.13893606601157477 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.068296516351614E-5 0.0 5 0.0 0.0 0.0 8.068296516351614E-5 0.0 6 0.0 0.0 0.0 8.068296516351614E-5 0.0 7 0.0 0.0 0.0 8.068296516351614E-5 0.0 8 0.0 0.0 0.0 8.068296516351614E-5 0.0 9 0.0 0.0 0.0 8.068296516351614E-5 0.0 10-11 0.0 0.0 0.0 8.068296516351614E-5 0.0 12-13 0.0 0.0 0.0 1.6136593032703227E-4 0.0 14-15 0.0 0.0 0.0 1.6136593032703227E-4 0.0 16-17 0.0 0.0 0.0 4.0341482581758066E-4 0.0 18-19 0.0 0.0 0.0 5.647807561446129E-4 0.0 20-21 0.0 0.0 0.0 7.664881690534033E-4 0.0 22-23 0.0 0.0 0.0 0.001210244477452742 0.0 24-25 0.0 0.0 0.0 0.0024608304374872424 0.0 26-27 0.0 0.0 0.0 0.005365417183373822 0.0 28-29 0.0 0.0 0.0 0.013514396664888953 0.0 30-31 0.0 0.0 0.0 0.03667040766681808 0.0 32-33 0.0 0.0 0.0 0.08649213865528929 0.0 34-35 0.0 0.0 0.0 0.1647949563464817 0.0 36-37 0.0 0.0 0.0 0.29683262883657585 0.0 38-39 4.034148258175807E-5 0.0 0.0 0.4860341821450212 0.0 40-41 8.068296516351614E-5 0.0 0.0 0.685321106098906 0.0 42-43 8.068296516351614E-5 0.0 0.0 0.8691169007413958 0.0 44-45 8.068296516351614E-5 0.0 0.0 1.0828460754595501 0.0 46-47 8.068296516351614E-5 0.0 0.0 1.3570471325677596 0.0 48-49 8.068296516351614E-5 0.0 0.0 1.6575104948366937 0.0 50-51 8.068296516351614E-5 0.0 0.0 1.9508737561712384 0.0 52-53 8.068296516351614E-5 0.0 0.0 2.2155542233901526 0.0 54-55 8.068296516351614E-5 0.0 0.0 2.4991145044573306 0.0 56-57 8.068296516351614E-5 0.0 0.0 2.8378215922137713 0.0 58-59 8.068296516351614E-5 0.0 0.0 3.199402300594069 0.0 60-61 8.068296516351614E-5 0.0 0.0 3.5724399900275854 0.0 62-63 8.068296516351614E-5 0.0 0.0 3.9247421574140784 0.0 64-65 8.068296516351614E-5 0.0 0.0 4.2847898894562695 0.0 66-67 8.068296516351614E-5 0.0 0.0 4.710876628484797 0.0 68-69 8.068296516351614E-5 0.0 0.0 5.1854538295766 0.0 70-71 8.068296516351614E-5 0.0 0.0 5.659345225464512 0.0 72-73 8.068296516351614E-5 0.0 0.0 6.13565711030733 0.0 74-75 8.068296516351614E-5 0.0 0.0 6.605272309041575 0.0 76-77 8.068296516351614E-5 0.0 0.0 7.084246731734789 0.0 78-79 8.068296516351614E-5 0.0 0.0 7.619820254490209 0.0 80-81 8.068296516351614E-5 0.0 0.0 8.182583936505734 0.0 82-83 8.068296516351614E-5 0.0 0.0 8.766930311702499 0.0 84-85 8.068296516351614E-5 0.0 0.0 9.330823555230314 0.0 86-87 8.068296516351614E-5 0.0 0.0 9.911619879959884 0.0 88-89 8.068296516351614E-5 0.0 0.0 10.582619759742265 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1975 0.0 59.645573 1 GTATCAA 3865 0.0 43.751617 1 TCAACGC 4485 0.0 37.068005 4 ATCAACG 4565 0.0 36.73056 3 AACGCAG 4660 0.0 36.08369 6 CAACGCA 4635 0.0 35.86839 5 TATCAAC 4750 0.0 35.4 2 CGCAGAG 5200 0.0 31.971155 8 ACGCAGA 5245 0.0 31.787418 7 GCAGAGT 5830 0.0 27.457117 9 GTACCGT 70 0.00839182 27.142859 6 GTACATG 3990 0.0 26.428572 1 TACATGG 3940 0.0 26.04061 2 ACATGGG 3960 0.0 25.309341 3 CAGAGTA 5475 0.0 24.335617 10-11 GAGTACT 3590 0.0 23.816156 12-13 GGACCGT 100 0.0016537191 23.749998 6 CATGGGG 2480 0.0 23.175404 4 AGAGTAC 5125 0.0 23.031708 10-11 TGGACCG 110 0.0028774922 21.59091 5 >>END_MODULE