FastQCFastQC Report
Fri 10 Feb 2017
SRR2031487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239419
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT61220.49394111273104574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.3078861950639776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.2586695863142327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.16185002811801336No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT19480.15717041613852942RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT12750.10287078058348308RNA PCR Primer, Index 33 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.058.865331
GTATCAA36000.041.238431
ATCAACG44650.033.0839543
TCAACGC44700.033.0469474
AACGCAG45300.032.3995326
CAACGCA45600.032.3947075
TATCAAC46750.032.004232
ACGCAGA50650.028.9772767
CGCAGAG50500.028.7811768
GCAGAGT57000.024.8324689
GTACATG31300.023.563371
AGAGTAC52150.022.4968210-11
TACATGG31550.022.4318662
GAGTACT39450.022.33445512-13
ACATGGG30450.022.1502973
GTATAGA4800.021.8087861
CAGAGTA55550.021.7611710-11
GTACTTT42750.020.38817814-15
GTATAGT4250.020.1527181
GTAACGC1200.004764664519.7909773
Produced by FastQC (version 0.11.5)