FastQCFastQC Report
Fri 10 Feb 2017
SRR2031486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966452
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.320760886210593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.24533034232429546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.14216950246882412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.058.412161
GTATCAA28950.045.613131
TAATGCG250.00161608938.078-79
TCAACGC35250.036.7872354
TATCAAC35800.036.487432
ATCAACG35700.036.323533
AACGCAG36250.035.903456
CAACGCA36350.035.8046765
ACGCAGA40350.032.255277
CGCAGAG40550.032.2133188
GCAGAGT46550.027.8571419
TATACGT950.001226413425.04
GTACATG31050.024.782611
TACATGG30050.024.6589012
ACATGGG29400.024.073133
CAGAGTA44450.023.66985310-11
AGAGTAC41900.023.2965410-11
GAGTACT30500.022.81557512-13
GTATAGT4300.022.0930231
CATGGGA14150.021.4840984
Produced by FastQC (version 0.11.5)