##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031486_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 966452 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19004151266695 34.0 31.0 34.0 30.0 34.0 2 32.34092225997773 34.0 31.0 34.0 30.0 34.0 3 32.44107519049058 34.0 31.0 34.0 30.0 34.0 4 35.682149760153635 37.0 35.0 37.0 35.0 37.0 5 35.67273180664947 37.0 35.0 37.0 35.0 37.0 6 35.80130311696804 37.0 37.0 37.0 35.0 37.0 7 35.83055030151523 37.0 37.0 37.0 35.0 37.0 8 35.87515572423669 37.0 37.0 37.0 35.0 37.0 9 37.69010669955673 39.0 39.0 39.0 35.0 39.0 10-11 37.68871397648306 39.0 38.5 39.0 35.0 39.0 12-13 37.65708281425255 39.0 39.0 39.0 35.0 39.0 14-15 39.15256888081353 41.0 39.0 41.0 36.0 41.0 16-17 39.170821209951455 41.0 39.0 41.0 36.0 41.0 18-19 39.142935707101856 41.0 39.5 41.0 36.0 41.0 20-21 39.111895365729495 41.0 39.0 41.0 36.0 41.0 22-23 39.039753138283125 41.0 39.0 41.0 36.0 41.0 24-25 38.98838742120664 41.0 39.0 41.0 35.5 41.0 26-27 38.88418410847099 41.0 39.0 41.0 35.0 41.0 28-29 38.78027413673933 41.0 39.0 41.0 35.0 41.0 30-31 38.60795259361044 40.0 38.5 41.0 34.5 41.0 32-33 38.48646440795818 40.0 38.0 41.0 34.0 41.0 34-35 38.348221639564095 40.0 38.0 41.0 34.0 41.0 36-37 38.20630408959782 40.0 38.0 41.0 34.0 41.0 38-39 38.06029839040118 40.0 38.0 41.0 33.0 41.0 40-41 37.93374994309081 40.0 38.0 41.0 33.0 41.0 42-43 37.78553358056065 40.0 38.0 41.0 33.0 41.0 44-45 37.91814906482681 40.0 38.0 41.0 33.0 41.0 46-47 37.76618911234081 40.0 38.0 41.0 32.5 41.0 48-49 37.61981246869995 40.0 38.0 41.0 32.0 41.0 50-51 36.576607012039915 39.0 36.5 40.0 30.5 40.5 52-53 36.6518740713455 39.5 37.0 40.0 30.5 40.5 54-55 37.441303344604805 40.0 38.0 41.0 32.0 41.0 56-57 37.496018943517115 40.0 37.0 41.0 32.0 41.0 58-59 37.32643783654025 40.0 37.0 41.0 31.0 41.0 60-61 37.180479216764 40.0 36.5 41.0 31.0 41.0 62-63 36.95102602095086 40.0 36.0 41.0 31.0 41.0 64-65 36.6995505208743 39.0 35.5 41.0 30.5 41.0 66-67 36.43739937420586 39.0 35.0 41.0 30.0 41.0 68-69 36.15677860876691 39.0 35.0 41.0 30.0 41.0 70-71 35.811995318960484 37.5 35.0 40.5 29.0 41.0 72-73 35.459618791207426 37.0 35.0 40.0 29.0 41.0 74-75 35.08882955387334 36.5 35.0 39.0 29.0 41.0 76-77 34.7068198937971 36.0 35.0 39.0 29.0 41.0 78-79 34.2978740796232 36.0 34.5 38.5 28.0 40.0 80-81 33.93644795602886 35.0 34.0 37.0 27.5 39.0 82-83 33.611670315752875 35.0 34.0 37.0 27.0 39.0 84-85 33.286936650759685 35.0 34.0 36.5 26.5 38.0 86-87 32.999260180536645 35.0 34.0 36.0 26.0 37.0 88-89 32.75668372562735 35.0 34.0 36.0 26.0 37.0 90-91 32.5429110809435 35.0 34.0 35.0 25.5 36.5 92-93 32.36842802332656 35.0 34.0 35.0 25.0 36.0 94-95 32.22286931994553 35.0 33.5 35.0 25.0 36.0 96-97 32.08135996407478 35.0 33.0 35.0 25.0 36.0 98-99 31.94718051181021 35.0 33.0 35.0 24.0 36.0 100-101 30.4405966359426 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2519648870613622 1101 2 0.21807843275424688 1101 3 0.2156491928881863 1101 4 0.004646898828482904 1101 5 0.2274931518442287 1101 6 0.18414676890328252 1101 7 0.30226401427623983 1101 8 0.31358880272892975 1101 9 0.45179737897150574 1101 10-11 0.595447205560987 1101 12-13 0.6537075474534859 1101 14-15 0.7742775203034 1101 16-17 0.6361350936324186 1101 18-19 0.7248436067837503 1101 20-21 0.8030553474466942 1101 22-23 0.6677902244582228 1101 24-25 0.6627470479801545 1101 26-27 0.5184149604820831 1101 28-29 0.6388549237445247 1101 30-31 0.7804808198682238 1101 32-33 0.7404493688523459 1101 34-35 0.5736218133812017 1101 36-37 0.5538421490813832 1101 38-39 0.7084300014938094 1101 40-41 0.6319163811857607 1101 42-43 0.6548233552539671 1101 44-45 0.5608273132720996 1101 46-47 0.8068318563743944 1101 48-49 0.8224604824036561 1101 50-51 0.8712898781130107 1101 52-53 0.8578077594905977 1101 54-55 0.660576772943017 1101 56-57 0.6940404867940799 1101 58-59 0.8029674463708574 1101 60-61 0.7852628350891564 1101 62-63 0.9061310631810855 1101 64-65 0.7790977087220554 1101 66-67 0.774338749872264 1101 68-69 0.6917775217328952 1101 70-71 0.7344874642802921 1101 72-73 0.6788682603226093 1101 74-75 0.814962290422578 1101 76-77 0.6196692699091031 1101 78-79 0.5269564688946176 1101 80-81 0.6002504168069365 1101 82-83 0.703382240217465 1101 84-85 0.868025036287122 1101 86-87 0.5606723630168631 1101 88-89 0.4376842643993868 1101 90-91 0.4272654969542842 1101 92-93 0.5621407216664167 1101 94-95 0.615900942344112 1101 96-97 0.678340876506951 1101 98-99 0.7477086058531413 1101 100-101 0.6918258972736027 1102 1 0.061404119260352275 1102 2 0.10724387607032071 1102 3 0.2948135689189755 1102 4 -0.09074222734678727 1102 5 0.030544782273537407 1102 6 0.09563257098831457 1102 7 0.019381055197563057 1102 8 0.23037478383596266 1102 9 0.22867031362343937 1102 10-11 0.24714267886173502 1102 12-13 0.29517721943442865 1102 14-15 0.35214705776611765 1102 16-17 0.42019105965650283 1102 18-19 0.3372086102977363 1102 20-21 0.3969603429459738 1102 22-23 0.4216641679968163 1102 24-25 0.4480746944952685 1102 26-27 0.5591137705596765 1102 28-29 0.44972693610087333 1102 30-31 0.5634413081613232 1102 32-33 0.542521311935829 1102 34-35 0.6137255615663122 1102 36-37 0.6685622283424166 1102 38-39 0.7960400275315465 1102 40-41 0.6142658989943257 1102 42-43 0.5980558603336448 1102 44-45 0.6282585992300511 1102 46-47 0.6573625790876392 1102 48-49 0.6016331147923353 1102 50-51 0.48705911895097387 1102 52-53 0.5240595945164799 1102 54-55 0.5489206079608522 1102 56-57 0.411768699574516 1102 58-59 0.6027719116508479 1102 60-61 0.6114242126524019 1102 62-63 0.5213646476285234 1102 64-65 0.5227277272283786 1102 66-67 0.53286041878426 1102 68-69 0.6218522115657024 1102 70-71 0.5205028049960134 1102 72-73 0.4710950000479741 1102 74-75 0.6036710161846273 1102 76-77 0.5620086787136032 1102 78-79 0.3552302658325104 1102 80-81 0.20455640951315246 1102 82-83 0.242903008985067 1102 84-85 0.4704421354057118 1102 86-87 0.15681664387802385 1102 88-89 0.22003156708753124 1102 90-91 0.21179267950235214 1102 92-93 0.34536098317145303 1102 94-95 0.24954419116053828 1102 96-97 0.2564097748067482 1102 98-99 0.41764358200031637 1102 100-101 0.47467059518152155 1103 1 0.11329403410479699 1103 2 0.24950638855194995 1103 3 0.37531305843440776 1103 4 0.19853984062059027 1103 5 0.1696546318208334 1103 6 0.2374911835922333 1103 7 0.29933976765450865 1103 8 0.3145430305739083 1103 9 0.43997726631106104 1103 10-11 0.5329298909428246 1103 12-13 0.3874010248444435 1103 14-15 0.4563195678916614 1103 16-17 0.4185249726609612 1103 18-19 0.464554875906785 1103 20-21 0.41363804206299903 1103 22-23 0.38729341034796505 1103 24-25 0.5593210622012279 1103 26-27 0.6509448955330299 1103 28-29 0.5310117864896284 1103 30-31 0.4681097175811644 1103 32-33 0.3216049079910732 1103 34-35 0.323983496516 1103 36-37 0.34800596845528986 1103 38-39 0.3481935992406022 1103 40-41 0.4840418467566039 1103 42-43 0.3996905328942546 1103 44-45 0.4817495283119655 1103 46-47 0.47416032622387405 1103 48-49 0.3125642162048621 1103 50-51 0.3790776085898173 1103 52-53 0.32687230838707393 1103 54-55 0.3647815317696299 1103 56-57 0.3064854647852755 1103 58-59 0.4248315766383399 1103 60-61 0.39527607115280716 1103 62-63 0.23681718378316674 1103 64-65 0.29416527420978156 1103 66-67 0.4265842634623169 1103 68-69 0.42353176189050146 1103 70-71 0.39074996928229666 1103 72-73 0.286495926773803 1103 74-75 0.37007664385625816 1103 76-77 0.361427511667344 1103 78-79 0.4831543326555092 1103 80-81 0.472291540948909 1103 82-83 0.37792898199109004 1103 84-85 0.47888476479391784 1103 86-87 0.509359530191432 1103 88-89 0.540817825516136 1103 90-91 0.4065341274834182 1103 92-93 0.44470913364659026 1103 94-95 0.3349577958547556 1103 96-97 0.33027857466314003 1103 98-99 0.44351000333521284 1103 100-101 0.4470202827200893 1104 1 0.15192327890616752 1104 2 0.16612544996961986 1104 3 0.2431001732803466 1104 4 0.06499952794379027 1104 5 0.01555161130228555 1104 6 -0.04817756666648876 1104 7 -0.03843396252721476 1104 8 -0.006549544387105755 1104 9 0.22281729887581037 1104 10-11 0.28140445318151563 1104 12-13 0.41794013704104316 1104 14-15 0.4412666501728566 1104 16-17 0.3752207544219388 1104 18-19 0.33729224671717617 1104 20-21 0.43595179515044435 1104 22-23 0.3641131037425609 1104 24-25 0.41559460610155696 1104 26-27 0.32724369091325656 1104 28-29 0.417049649910858 1104 30-31 0.43194490682927267 1104 32-33 0.4404779744590357 1104 34-35 0.45235484505540313 1104 36-37 0.4329392521135773 1104 38-39 0.29841023762009655 1104 40-41 0.40834536126585874 1104 42-43 0.4769510922904274 1104 44-45 0.5349418397192878 1104 46-47 0.5080124877781529 1104 48-49 0.5773156340133667 1104 50-51 0.32449370606329353 1104 52-53 0.44090964746063577 1104 54-55 0.46675818449968176 1104 56-57 0.4799431237109104 1104 58-59 0.6127453628424817 1104 60-61 0.5435298554251418 1104 62-63 0.6284200318189406 1104 64-65 0.6001566362718549 1104 66-67 0.4749732742390407 1104 68-69 0.32368837626038527 1104 70-71 0.34675926955412706 1104 72-73 0.3596582951694387 1104 74-75 0.2906007155539001 1104 76-77 0.45854428941292724 1104 78-79 0.4648614982803778 1104 80-81 0.5841038520802542 1104 82-83 0.46721435131124167 1104 84-85 0.6582289162475945 1104 86-87 0.6157979676353733 1104 88-89 0.6066627841892611 1104 90-91 0.49572911582357904 1104 92-93 0.23655690723878564 1104 94-95 0.3855607956753744 1104 96-97 0.30380246697868074 1104 98-99 0.2765742635220434 1104 100-101 0.3597902182805228 1105 1 0.21486799702329762 1105 2 0.21869406362197452 1105 3 0.20364439096741904 1105 4 0.24282422103942025 1105 5 0.18952924833410378 1105 6 0.1418529282901133 1105 7 0.241783411778826 1105 8 0.1444083100190241 1105 9 0.2878497486373206 1105 10-11 0.3269474979856426 1105 12-13 0.3605236072107161 1105 14-15 0.17827501673786372 1105 16-17 0.2338844497851369 1105 18-19 0.20691848801830304 1105 20-21 0.16750625601525115 1105 22-23 0.1627369210855889 1105 24-25 0.1615286118877748 1105 26-27 0.22317378708965663 1105 28-29 0.1645318611861697 1105 30-31 0.2257794521416514 1105 32-33 0.16837695014127263 1105 34-35 0.27446368859282444 1105 36-37 0.12094847879275505 1105 38-39 0.2477534231701739 1105 40-41 0.08704971657119387 1105 42-43 0.21738427705858498 1105 44-45 0.22611394074360902 1105 46-47 0.09967719963990618 1105 48-49 0.01281098157767957 1105 50-51 0.0894284155792846 1105 52-53 0.05586390502573835 1105 54-55 0.07406524525502078 1105 56-57 0.15455802714355116 1105 58-59 0.07289080032781925 1105 60-61 0.04363541489030354 1105 62-63 0.12477236585599627 1105 64-65 0.1371024490797339 1105 66-67 0.17595067669427777 1105 68-69 0.09876852326171814 1105 70-71 0.09829965582357403 1105 72-73 -9.52385884588125E-4 1105 74-75 6.197132057650379E-5 1105 76-77 -0.07277950447244308 1105 78-79 0.09206456341447478 1105 80-81 0.08323591870000513 1105 82-83 0.11138903267803357 1105 84-85 0.15932360699746084 1105 86-87 0.15088280616848238 1105 88-89 0.12054971827323868 1105 90-91 0.08033698195853844 1105 92-93 0.15992855474836887 1105 94-95 0.03136969394732603 1105 96-97 0.05866993120575614 1105 98-99 0.21411824663253043 1105 100-101 -0.02997051359843539 1106 1 0.12666236062489133 1106 2 0.08313140482351855 1106 3 0.14065402505106306 1106 4 -0.032282389695417635 1106 5 -0.0596331402430863 1106 6 0.06063007770409712 1106 7 0.10473180398711435 1106 8 0.17428539411297095 1106 9 0.25708351735473656 1106 10-11 0.049546614302485636 1106 12-13 0.2324181116353472 1106 14-15 0.139494042500786 1106 16-17 0.19159345525662275 1106 18-19 0.22638907289335464 1106 20-21 0.15769645403677401 1106 22-23 0.04744706655305464 1106 24-25 0.16092073821621256 1106 26-27 0.20042229876157336 1106 28-29 0.2187574214358321 1106 30-31 0.24980443270708008 1106 32-33 0.10753680539406929 1106 34-35 0.1946695584544571 1106 36-37 0.2548576466950365 1106 38-39 0.17512524813236752 1106 40-41 0.1279548336381069 1106 42-43 0.11264559443694111 1106 44-45 0.20100546727024238 1106 46-47 0.11219976982168589 1106 48-49 0.23541133972020134 1106 50-51 0.2649294301508647 1106 52-53 0.2803816420219576 1106 54-55 0.4027044775087063 1106 56-57 0.35644411199005077 1106 58-59 0.3503755072445358 1106 60-61 0.4655363768084797 1106 62-63 0.426718555065527 1106 64-65 0.5603799747668958 1106 66-67 0.5767685430887326 1106 68-69 0.4678732662907308 1106 70-71 0.4897330455357931 1106 72-73 0.6577579036346464 1106 74-75 0.6505886295924981 1106 76-77 0.5676182026206149 1106 78-79 0.46963545303356113 1106 80-81 0.5142866522464331 1106 82-83 0.44881976985060135 1106 84-85 0.6613794494147527 1106 86-87 0.6121130083625417 1106 88-89 0.6356583904387136 1106 90-91 0.591415308871639 1106 92-93 0.506892166772225 1106 94-95 0.5517207332312353 1106 96-97 0.6897191745613682 1106 98-99 0.5029153646034885 1106 100-101 0.673813080890838 1107 1 0.057883354123163144 1107 2 0.17121505034819506 1107 3 0.2508285950932745 1107 4 0.22377463659748287 1107 5 0.050470229999433514 1107 6 0.035802707551766844 1107 7 0.010865548064181496 1107 8 0.18311060928470368 1107 9 0.16602846685628236 1107 10-11 0.0766784846381654 1107 12-13 0.11647813781313943 1107 14-15 0.2114177068222105 1107 16-17 0.14215765433601746 1107 18-19 0.142641495169876 1107 20-21 0.2689912342607954 1107 22-23 0.2213240973790036 1107 24-25 0.12288343189450757 1107 26-27 0.08181648577259182 1107 28-29 0.2450835473716637 1107 30-31 0.2283596463717501 1107 32-33 0.3516702385653403 1107 34-35 0.31862425639524616 1107 36-37 0.4195932165723022 1107 38-39 0.3651759067898439 1107 40-41 0.3297261659880917 1107 42-43 0.2517154165205113 1107 44-45 0.08076443221501961 1107 46-47 0.28662919242690776 1107 48-49 0.22359276630312763 1107 50-51 0.32261016787572316 1107 52-53 0.19787531413975756 1107 54-55 0.23776539487066373 1107 56-57 0.2815003894983832 1107 58-59 0.26261522747680033 1107 60-61 0.2623303684947089 1107 62-63 0.4111020656665829 1107 64-65 0.3910621044478404 1107 66-67 0.41013846520802133 1107 68-69 0.34137495550597663 1107 70-71 0.2317364180329733 1107 72-73 0.21270931570467866 1107 74-75 0.2112828988392934 1107 76-77 0.20490921637523485 1107 78-79 0.030068656348142042 1107 80-81 0.2770821462484463 1107 82-83 0.13632681566410554 1107 84-85 0.25340519051566446 1107 86-87 0.37356935279869674 1107 88-89 0.2832751551435493 1107 90-91 0.32739933163657753 1107 92-93 0.35247700786019465 1107 94-95 0.20963832895640166 1107 96-97 0.2642606181847711 1107 98-99 0.22691112109541933 1107 100-101 0.24889682965522297 1108 1 0.20338591366944314 1108 2 0.1985672952310651 1108 3 0.24916704677224288 1108 4 0.05359085792512985 1108 5 0.20363182145573688 1108 6 0.13493764925192409 1108 7 0.10157958809147516 1108 8 0.18192410916874024 1108 9 0.2558047348372483 1108 10-11 0.19435599188992825 1108 12-13 0.27030020313002723 1108 14-15 0.3540565323775624 1108 16-17 0.15767626916715471 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-0.5981088107729668 2316 92-93 -0.6137737189539081 2316 94-95 -0.5748017836527239 2316 96-97 -0.5857040345967803 2316 98-99 -0.6106932996075898 2316 100-101 -0.42530053749988994 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 266.0 8 566.0 9 483.0 10 544.0 11 726.0 12 1068.0 13 1203.0 14 1442.0 15 1638.0 16 1897.0 17 2458.0 18 3041.0 19 3877.0 20 4843.0 21 6303.0 22 7457.0 23 7475.0 24 7026.0 25 6441.0 26 6313.0 27 6724.0 28 7719.0 29 8937.0 30 10584.0 31 13067.0 32 16172.0 33 21514.0 34 31107.0 35 47979.0 36 88775.0 37 191157.0 38 318354.0 39 139062.0 40 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69875793107159 20.51493504074698 14.285758630537265 22.500548397644167 2 14.131793405156179 21.435725726678616 42.4351131768572 21.997367691308 3 16.71909210183227 28.36229838626233 31.757500631174647 23.161108880730755 4 11.096464180321423 18.657832980841263 42.15377483827443 28.09192800056288 5 11.693907198702057 39.45131263632338 36.728156183649055 12.126623981325508 6 27.088670725499043 40.92370857528361 19.742625603754764 12.244995095462578 7 24.418181140915433 34.46637805084991 24.796265101629466 16.31917570660519 8 23.930210708860862 37.02046247511516 21.85478430382471 17.19454251219926 9 25.494178707271548 16.616552089498494 21.781319713757124 36.10794948947284 10-11 22.742153774838275 27.498572096700098 30.82243091224396 18.936843216217667 12-13 24.170367488504343 25.340523895651312 30.281431462711026 20.207677153133314 14-15 21.33711762198226 26.614203292041406 28.178533439839743 23.87014564613659 16-17 20.551719071407582 29.868167275767448 28.425364115341477 21.154749537483497 18-19 20.486118296614837 28.885862929560908 30.63432017316949 19.993698600654767 20-21 21.427551497642924 28.054471406753777 30.557492767359374 19.960484328243925 22-23 21.135452148684053 27.998441722920536 30.163681176095658 20.702424952299754 24-25 20.855976292666373 28.14040428288213 30.261409775136272 20.742209649315228 26-27 20.78654708148982 28.48211809795003 30.059226945569982 20.67210787499017 28-29 20.75276371718409 28.428106103562307 30.231920467855623 20.587209711397982 30-31 21.175547259460377 28.293179588846627 30.1411244428073 20.390148708885697 32-33 20.876618807762828 28.318271367848585 30.14640147674173 20.658708347646858 34-35 21.08480297003886 28.340155537988437 30.024771018115747 20.55027047385695 36-37 21.05593449027991 28.278590142086724 29.970293403086757 20.69518196454661 38-39 20.963534660800537 28.507572026339645 29.94090756705972 20.587985745800104 40-41 21.048225881885493 28.395564394299978 29.92316224706452 20.63304747675001 42-43 20.89395024274356 28.37968155686987 29.86118296614835 20.865185234238222 44-45 20.974761291817906 28.50570954377455 29.672451399552173 20.847077764855367 46-47 21.09792482905229 28.48987092458434 29.406110297423403 21.00609394893996 48-49 20.908149787598614 28.70266755031437 29.41398325932525 20.97519940276176 50-51 20.977296337531506 28.811725776344815 29.150180246923803 21.060797639199876 52-53 21.05764176596458 28.815502477101813 29.115413905708714 21.011441851224895 54-55 20.896433552830352 28.71627354488376 29.23988982380915 21.14740307847674 56-57 21.045949514305935 28.752022863008197 29.083027403326806 21.11900021935906 58-59 20.98593618720847 28.652224838895258 29.130468973109892 21.23137000078638 60-61 21.11087772595018 28.64777557498976 29.17128838266153 21.070058316398537 62-63 20.90797059760857 28.576121731860454 29.318424505303938 21.197483165227037 64-65 20.911488620231527 28.38863182030768 29.341705537367606 21.35817402209318 66-67 21.022668482242263 28.543010930703232 29.091770724257387 21.342549862797117 68-69 21.040931158505543 28.506795991937523 29.069317462222642 21.38295538733429 70-71 21.065971201880693 28.322410217993237 29.131503685646052 21.480114894480014 72-73 20.977606751292356 28.458061031484235 28.96703612802291 21.597296089200498 74-75 20.915472263495754 28.41962146076577 28.982298137931316 21.682608137807154 76-77 20.914334079705977 28.4261401497436 28.967191334903337 21.69233443564709 78-79 20.985729244701236 28.385889832086846 28.88922574530344 21.739155177908472 80-81 21.022927160376305 28.52179932371189 28.744831610881867 21.710441905029946 82-83 21.119414104373526 28.404876807125447 28.80174080037084 21.67396828813019 84-85 21.1953620045279 28.294524715143638 28.7888068936688 21.72130638665966 86-87 21.071817327710015 28.366230293899747 28.820210419141358 21.74174195924888 88-89 21.217970473443067 28.0914106442948 28.885086895158786 21.805531987103343 90-91 21.23395678212679 28.14061122538936 28.88669069958984 21.73874129289401 92-93 21.399200374152052 28.07154416360047 28.743848633972508 21.78540682827497 94-95 21.257444756697694 27.937135005152868 28.86987662087719 21.93554361727225 96-97 21.340894322739256 27.821143729848973 28.905988088389282 21.931973859022484 98-99 21.298729786890604 27.79946650221635 28.812243132612895 22.089560578280143 100-101 21.579654240458915 27.734434819318498 28.81927917785881 21.86663176236378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 40.0 1 33.0 2 62.5 3 97.5 4 87.0 5 93.0 6 142.0 7 306.5 8 596.5 9 746.5 10 674.0 11 608.5 12 659.5 13 741.5 14 793.0 15 881.0 16 966.0 17 1092.0 18 1246.5 19 1400.0 20 1632.5 21 1954.0 22 2482.5 23 3200.0 24 4049.0 25 5200.0 26 7218.5 27 9934.0 28 12952.5 29 16414.5 30 19675.5 31 22618.0 32 25705.0 33 28205.0 34 30068.5 35 31506.5 36 33068.5 37 34156.5 38 34073.5 39 33609.0 40 33581.0 41 33805.0 42 33548.5 43 33660.5 44 33996.0 45 34313.0 46 34336.0 47 33939.0 48 33369.0 49 32437.0 50 31216.5 51 29806.0 52 28047.0 53 26099.0 54 23896.0 55 21434.5 56 18775.5 57 16089.0 58 13943.5 59 11874.5 60 9782.0 61 7787.0 62 5962.0 63 4455.5 64 3146.0 65 2241.5 66 1647.0 67 1174.5 68 830.5 69 567.0 70 437.5 71 339.0 72 226.0 73 165.0 74 127.0 75 99.5 76 75.0 77 58.0 78 40.0 79 31.0 80 27.5 81 18.0 82 13.0 83 10.5 84 12.0 85 8.5 86 6.5 87 8.0 88 4.0 89 3.0 90 3.0 91 1.5 92 2.5 93 1.5 94 0.5 95 0.5 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.5520688042448046E-4 48-49 3.6214938765712106E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 966452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.72045003443976 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43728908969177 53.90245805725071 2 13.469067125270856 18.512007087853824 3 4.196297520908816 8.651143623457735 4 1.6559369089601534 4.551869184495234 5 0.7912398243470944 2.71871784071517 6 0.4324388876478149 1.7830436982930224 7 0.27155822564736926 1.306312243392881 8 0.1687797770760556 0.9278897789903169 9 0.11643134128954834 0.7201092754378089 >10 0.4397365014712722 4.908331753557803 >50 0.014556679995461074 0.6817352265987231 >100 0.0062134736917932665 0.6235133461552995 >500 0.0 0.0 >1k 4.546440018845783E-4 0.7128688838014948 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3100 0.320760886210593 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2371 0.24533034232429546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.14216950246882412 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.173562680816016E-5 0.0 12-13 0.0 0.0 0.0 1.0347125361632031E-4 0.0 14-15 0.0 0.0 0.0 2.0694250723264063E-4 5.173562680816016E-5 16-17 0.0 0.0 0.0 5.173562680816016E-4 1.0347125361632031E-4 18-19 0.0 0.0 0.0 8.277700289305625E-4 1.0347125361632031E-4 20-21 0.0 0.0 0.0 0.0013968619238203242 1.0347125361632031E-4 22-23 0.0 0.0 0.0 0.0028454594744488086 1.0347125361632031E-4 24-25 0.0 0.0 0.0 0.003983643264228332 1.0347125361632031E-4 26-27 0.0 0.0 0.0 0.005690918948897617 1.0347125361632031E-4 28-29 0.0 0.0 0.0 0.010088447227591231 1.0347125361632031E-4 30-31 0.0 0.0 0.0 0.022608468915165988 1.0347125361632031E-4 32-33 0.0 0.0 0.0 0.05287381059793968 1.0347125361632031E-4 34-35 0.0 0.0 0.0 0.09819421968188798 1.0347125361632031E-4 36-37 0.0 0.0 0.0 0.17512509674562216 1.0347125361632031E-4 38-39 0.0 0.0 0.0 0.29742811852011275 1.0347125361632031E-4 40-41 0.0 0.0 0.0 0.4285779324787987 1.0347125361632031E-4 42-43 0.0 0.0 0.0 0.5534159999668892 1.0347125361632031E-4 44-45 0.0 0.0 0.0 0.7035010533373618 1.0347125361632031E-4 46-47 0.0 0.0 0.0 0.8907840223829016 2.0694250723264063E-4 48-49 0.0 0.0 0.0 1.093122058829616 2.0694250723264063E-4 50-51 0.0 0.0 0.0 1.3059106918915786 2.0694250723264063E-4 52-53 0.0 0.0 0.0 1.5071105445485136 2.0694250723264063E-4 54-55 0.0 0.0 0.0 1.7080517190714075 2.0694250723264063E-4 56-57 0.0 0.0 0.0 1.9418450166174832 2.586781340408008E-4 58-59 0.0 0.0 0.0 2.199178024361272 3.104137608489609E-4 60-61 0.0 0.0 0.0 2.478860822886186 3.104137608489609E-4 62-63 1.0347125361632031E-4 0.0 0.0 2.751817989926039 3.104137608489609E-4 64-65 1.0347125361632031E-4 0.0 0.0 3.0189290311365697 3.104137608489609E-4 66-67 1.0347125361632031E-4 0.0 0.0 3.3173918621928458 3.104137608489609E-4 68-69 1.0347125361632031E-4 0.0 0.0 3.6650035387168733 3.104137608489609E-4 70-71 1.0347125361632031E-4 0.0 0.0 4.033412937217782 3.104137608489609E-4 72-73 1.0347125361632031E-4 0.0 0.0 4.399235554378283 3.104137608489609E-4 74-75 1.0347125361632031E-4 0.0 0.0 4.751658644195469 3.104137608489609E-4 76-77 1.0347125361632031E-4 0.0 0.0 5.104961239668395 3.104137608489609E-4 78-79 1.0347125361632031E-4 0.0 0.0 5.505446726790363 3.104137608489609E-4 80-81 1.0347125361632031E-4 0.0 0.0 5.944371784630794 3.104137608489609E-4 82-83 1.0347125361632031E-4 0.0 0.0 6.405387955118309 3.104137608489609E-4 84-85 1.0347125361632031E-4 0.0 0.0 6.835517956401352 3.104137608489609E-4 86-87 1.0347125361632031E-4 0.0 0.0 7.266993083981408 3.104137608489609E-4 88-89 1.0347125361632031E-4 0.0 0.0 7.764638078249101 4.1388501446528125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 58.41216 1 GTATCAA 2895 0.0 45.61313 1 TAATGCG 25 0.001616089 38.0 78-79 TCAACGC 3525 0.0 36.787235 4 TATCAAC 3580 0.0 36.48743 2 ATCAACG 3570 0.0 36.32353 3 AACGCAG 3625 0.0 35.90345 6 CAACGCA 3635 0.0 35.804676 5 ACGCAGA 4035 0.0 32.25527 7 CGCAGAG 4055 0.0 32.213318 8 GCAGAGT 4655 0.0 27.857141 9 TATACGT 95 0.0012264134 25.0 4 GTACATG 3105 0.0 24.78261 1 TACATGG 3005 0.0 24.658901 2 ACATGGG 2940 0.0 24.07313 3 CAGAGTA 4445 0.0 23.669853 10-11 AGAGTAC 4190 0.0 23.29654 10-11 GAGTACT 3050 0.0 22.815575 12-13 GTATAGT 430 0.0 22.093023 1 CATGGGA 1415 0.0 21.484098 4 >>END_MODULE