FastQCFastQC Report
Fri 10 Feb 2017
SRR2031486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966452
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38830.4017788777921718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.3084478070302509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.1768323724302914No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16510.17083103972054484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.062.2093771
GTATCAA31850.041.3523141
CAACGCA38200.033.4478955
TCAACGC38350.033.317074
ATCAACG38650.033.181363
AACGCAG39500.032.5875786
TATCAAC41200.031.2429472
ACGCAGA43600.029.4142077
CGCAGAG43900.029.1050038
GCAGAGT48650.025.6775049
TGGTATC6850.024.9627152
GGACCGT1351.3427694E-524.628856
GAGTACT34500.023.88692712-13
CAGAGTA48600.023.70038210-11
GTACATG26750.023.6405371
ACATGGG24800.022.9831453
AGAGTAC45200.022.17297210-11
TACATGG26800.022.1541522
GTGGTAT8250.021.3244231
GTACTTT39400.020.73538614-15
Produced by FastQC (version 0.11.5)