FastQCFastQC Report
Fri 10 Feb 2017
SRR2031484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151268
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25620.22253723720280597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.1732003321555016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.052.355661
GTATCAA32000.049.2365151
ATCAACG38450.040.3669743
TCAACGC39100.039.6959154
CAACGCA39400.039.63465
TATCAAC39800.039.2362632
AACGCAG40300.038.8486446
ACGCAGA47250.033.13447
CGCAGAG48000.032.3201568
GTACATG46600.028.3111881
TACATGG47450.027.808872
GCAGAGT55200.027.7619089
ACATGGG46150.027.3580253
TCGCGAA350.00844397327.10937114-15
AGAGTAC48550.023.40464810-11
CATGGGG27050.022.8113444
GAGTACT28800.022.48671512-13
CAGAGTA52450.021.75481610-11
GTGTACG1404.8815386E-420.3386651
CATGGGA20850.019.5779484
Produced by FastQC (version 0.11.5)